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Protein

Sn1-specific diacylglycerol lipase beta

Gene

DAGLB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.1 Publication

Cofactori

Ca2+1 Publication

Enzyme regulationi

Inhibited by p-hydroxy-mercuri-benzoate and HgCl2, but not to PMSF. Also inhibited by RHC80267, a drug that blocks 2-AG formation.1 Publication

Kineticsi

  1. KM=74.1 µM for diacylglycerol1 Publication
  1. Vmax=3.45 nmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei443Charge relay system1
Active sitei495Charge relay system1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-426048 Arachidonate production from DAG

Protein family/group databases

ESTHERihuman-DAGLB Lipase_3

Chemistry databases

SwissLipidsiSLP:000000322

Names & Taxonomyi

Protein namesi
Recommended name:
Sn1-specific diacylglycerol lipase beta (EC:3.1.1.-)
Short name:
DGL-beta
Alternative name(s):
KCCR13L
Gene namesi
Name:DAGLB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000164535.14
HGNCiHGNC:28923 DAGLB
MIMi614016 gene
neXtProtiNX_Q8NCG7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 17CytoplasmicSequence analysisAdd BLAST17
Transmembranei18 – 38HelicalSequence analysisAdd BLAST21
Topological domaini39 – 58ExtracellularSequence analysisAdd BLAST20
Transmembranei59 – 79HelicalSequence analysisAdd BLAST21
Topological domaini80 – 102CytoplasmicSequence analysisAdd BLAST23
Transmembranei103 – 123HelicalSequence analysisAdd BLAST21
Topological domaini124 – 132ExtracellularSequence analysis9
Transmembranei133 – 153HelicalSequence analysisAdd BLAST21
Topological domaini154 – 672CytoplasmicSequence analysisAdd BLAST519

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi443S → A: Loss of activity. 1 Publication1
Mutagenesisi495D → A: Loss of activity. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000164535
PharmGKBiPA162383203

Chemistry databases

ChEMBLiCHEMBL5521
GuidetoPHARMACOLOGYi1397

Polymorphism and mutation databases

BioMutaiDAGLB
DMDMi114149272

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002483501 – 672Sn1-specific diacylglycerol lipase betaAdd BLAST672

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei570PhosphoserineCombined sources1
Modified residuei574PhosphoserineCombined sources1
Modified residuei579PhosphoserineCombined sources1
Modified residuei583PhosphoserineBy similarity1
Modified residuei584PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8NCG7
MaxQBiQ8NCG7
PaxDbiQ8NCG7
PeptideAtlasiQ8NCG7
PRIDEiQ8NCG7

PTM databases

iPTMnetiQ8NCG7
PhosphoSitePlusiQ8NCG7
SwissPalmiQ8NCG7

Expressioni

Gene expression databases

BgeeiENSG00000164535
CleanExiHS_DAGLB
ExpressionAtlasiQ8NCG7 baseline and differential
GenevisibleiQ8NCG7 HS

Organism-specific databases

HPAiHPA069377

Interactioni

Protein-protein interaction databases

BioGridi128772, 34 interactors
IntActiQ8NCG7, 4 interactors
STRINGi9606.ENSP00000297056

Chemistry databases

BindingDBiQ8NCG7

Structurei

3D structure databases

ProteinModelPortaliQ8NCG7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2088 Eukaryota
ENOG410XRFI LUCA
GeneTreeiENSGT00530000063682
HOGENOMiHOG000246993
HOVERGENiHBG055573
InParanoidiQ8NCG7
KOiK13806
OMAiATSVWET
OrthoDBiEOG091G04AM
PhylomeDBiQ8NCG7
TreeFamiTF312928

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002921 Fungal_lipase-like
PfamiView protein in Pfam
PF01764 Lipase_3, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00120 LIPASE_SER, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NCG7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPGMVLFGRR WAIASDDLVF PGFFELVVRV LWWIGILTLY LMHRGKLDCA
60 70 80 90 100
GGALLSSYLI VLMILLAVVI CTVSAIMCVS MRGTICNPGP RKSMSKLLYI
110 120 130 140 150
RLALFFPEMV WASLGAAWVA DGVQCDRTVV NGIIATVVVS WIIIAATVVS
160 170 180 190 200
IIIVFDPLGG KMAPYSSAGP SHLDSHDSSQ LLNGLKTAAT SVWETRIKLL
210 220 230 240 250
CCCIGKDDHT RVAFSSTAEL FSTYFSDTDL VPSDIAAGLA LLHQQQDNIR
260 270 280 290 300
NNQEPAQVVC HAPGSSQEAD LDAELENCHH YMQFAAAAYG WPLYIYRNPL
310 320 330 340 350
TGLCRIGGDC CRSRTTDYDL VGGDQLNCHF GSILHTTGLQ YRDFIHVSFH
360 370 380 390 400
DKVYELPFLV ALDHRKESVV VAVRGTMSLQ DVLTDLSAES EVLDVECEVQ
410 420 430 440 450
DRLAHKGISQ AARYVYQRLI NDGILSQAFS IAPEYRLVIV GHSLGGGAAA
460 470 480 490 500
LLATMLRAAY PQVRCYAFSP PRGLWSKALQ EYSQSFIVSL VLGKDVIPRL
510 520 530 540 550
SVTNLEDLKR RILRVVAHCN KPKYKILLHG LWYELFGGNP NNLPTELDGG
560 570 580 590 600
DQEVLTQPLL GEQSLLTRWS PAYSFSSDSP LDSSPKYPPL YPPGRIIHLQ
610 620 630 640 650
EEGASGRFGC CSAAHYSAKW SHEAEFSKIL IGPKMLTDHM PDILMRALDS
660 670
VVSDRAACVS CPAQGVSSVD VA
Length:672
Mass (Da):73,732
Last modified:September 5, 2006 - v2
Checksum:iAA953D737E0EFF44
GO
Isoform 2 (identifier: Q8NCG7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-281: Missing.

Note: No experimental confirmation available.
Show »
Length:391
Mass (Da):43,191
Checksum:i4DAEA2A26417EA69
GO
Isoform 3 (identifier: Q8NCG7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     249-267: IRNNQEPAQVVCHAPGSSQ → TRATGNCPRNDGLTLLSLN
     268-672: Missing.

Note: No experimental confirmation available.
Show »
Length:267
Mass (Da):28,899
Checksum:i661A57D0B8F4CB6A
GO
Isoform 4 (identifier: Q8NCG7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     140-310: SWIIIAATVV...TGLCRIGGDC → RTQIWCPATL...WSAMPQGAPS

Note: No experimental confirmation available.
Show »
Length:543
Mass (Da):59,837
Checksum:iBD549DBC7456917F
GO

Sequence cautioni

The sequence BAB85017 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti270D → Y in BAC11175 (PubMed:16303743).Curated1
Sequence conflicti456L → V in BAC04258 (PubMed:14702039).Curated1
Sequence conflicti545T → K in AAH27603 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027275664Q → R2 PublicationsCorresponds to variant dbSNP:rs2303361Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0202451 – 281Missing in isoform 2. 1 PublicationAdd BLAST281
Alternative sequenceiVSP_043309140 – 310SWIII…IGGDC → RTQIWCPATLRRASPCFISN RTISGTTKSLPRWSAMPQGA PS in isoform 4. 1 PublicationAdd BLAST171
Alternative sequenceiVSP_020246249 – 267IRNNQ…PGSSQ → TRATGNCPRNDGLTLLSLN in isoform 3. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_020247268 – 672Missing in isoform 3. 1 PublicationAdd BLAST405

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF450090 mRNA Translation: AAL47020.1
AK093958 mRNA Translation: BAC04258.1
AK074210 mRNA Translation: BAB85017.1 Different initiation.
AK122748 mRNA Translation: BAG53702.1
AK298955 mRNA Translation: BAG61052.1
AK074584 mRNA Translation: BAC11073.1
AK074744 mRNA Translation: BAC11175.1
AK075128 mRNA Translation: BAC11420.1
AC009412 Genomic DNA No translation available.
AC072052 Genomic DNA No translation available.
CH878731 Genomic DNA Translation: EAW55033.1
CH878731 Genomic DNA Translation: EAW55035.1
CH236963 Genomic DNA Translation: EAL23721.1
BC027603 mRNA Translation: AAH27603.1
CCDSiCCDS47536.1 [Q8NCG7-4]
CCDS5350.1 [Q8NCG7-1]
RefSeqiNP_001136408.1, NM_001142936.1 [Q8NCG7-4]
NP_631918.3, NM_139179.3 [Q8NCG7-1]
UniGeneiHs.487498

Genome annotation databases

EnsembliENST00000297056; ENSP00000297056; ENSG00000164535 [Q8NCG7-1]
ENST00000425398; ENSP00000391171; ENSG00000164535 [Q8NCG7-4]
GeneIDi221955
KEGGihsa:221955
UCSCiuc003sqa.4 human [Q8NCG7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDGLB_HUMAN
AccessioniPrimary (citable) accession number: Q8NCG7
Secondary accession number(s): A4D2P3
, B3KV90, B4DQU0, Q6PIX3, Q8N2N2, Q8N9S1, Q8TED3, Q8WXE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: April 25, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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