Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Carbohydrate sulfotransferase 4

Gene

CHST4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.1 Publication

Miscellaneous

May serve as an antiinflammatory target.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi51 – 57PAPSBy similarity7
Nucleotide bindingi205 – 213PAPSBy similarity9

GO - Molecular functioni

  • N-acetylglucosamine 6-O-sulfotransferase activity Source: UniProtKB
  • sulfotransferase activity Source: ProtInc

GO - Biological processi

  • carbohydrate metabolic process Source: UniProtKB-KW
  • cell adhesion Source: ProtInc
  • cell-cell signaling Source: ProtInc
  • immune response Source: ProtInc
  • inflammatory response Source: UniProtKB-KW
  • keratan sulfate biosynthetic process Source: GO_Central
  • movement of cell or subcellular component Source: ProtInc
  • N-acetylglucosamine metabolic process Source: UniProtKB
  • O-glycan processing Source: Reactome
  • protein sulfation Source: ProtInc
  • sulfur compound metabolic process Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processCarbohydrate metabolism, Inflammatory response

Enzyme and pathway databases

ReactomeiR-HSA-913709. O-linked glycosylation of mucins.
SABIO-RKiQ8NCG5.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 4 (EC:2.8.2.-)
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3
Short name:
GST-3
High endothelial cells N-acetylglucosamine 6-O-sulfotransferase
Short name:
HEC-GlcNAc6ST
L-selectin ligand sulfotransferase
Short name:
LSST
N-acetylglucosamine 6-O-sulfotransferase 2
Short name:
GlcNAc6ST-2
Short name:
Gn6st-2
Gene namesi
Name:CHST4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000140835.9.
HGNCiHGNC:1972. CHST4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini29 – 386LumenalSequence analysisAdd BLAST358

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10164.
OpenTargetsiENSG00000140835.
PharmGKBiPA26504.

Chemistry databases

ChEMBLiCHEMBL2239.

Polymorphism and mutation databases

BioMutaiCHST4.
DMDMi61211834.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000851931 – 386Carbohydrate sulfotransferase 4Add BLAST386

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi30N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi308N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi329N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ8NCG5.
PaxDbiQ8NCG5.
PeptideAtlasiQ8NCG5.
PRIDEiQ8NCG5.

PTM databases

iPTMnetiQ8NCG5.
PhosphoSitePlusiQ8NCG5.

Expressioni

Tissue specificityi

Specifically expressed in HEV. Weakly expressed in spleen. Not expressed in other tissues. Expressed in colonic mucinous adenocarcinoma.3 Publications

Inductioni

Upon cytokine activation, it is expressed at low level.1 Publication

Gene expression databases

BgeeiENSG00000140835.
CleanExiHS_CHST4.
GenevisibleiQ8NCG5. HS.

Organism-specific databases

HPAiHPA021955.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi115466. 2 interactors.
IntActiQ8NCG5. 1 interactor.
STRINGi9606.ENSP00000341206.

Structurei

3D structure databases

ProteinModelPortaliQ8NCG5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJXD. Eukaryota.
ENOG4110V0B. LUCA.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG050949.
InParanoidiQ8NCG5.
KOiK04746.
OMAiMQVICQS.
OrthoDBiEOG091G0V3Y.
PhylomeDBiQ8NCG5.
TreeFamiTF342871.

Family and domain databases

InterProiView protein in InterPro
IPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
PfamiView protein in Pfam
PF00685. Sulfotransfer_1. 1 hit.
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8NCG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLPKKMKLL LFLVSQMAIL ALFFHMYSHN ISSLSMKAQP ERMHVLVLSS
60 70 80 90 100
WRSGSSFVGQ LFGQHPDVFY LMEPAWHVWM TFKQSTAWML HMAVRDLIRA
110 120 130 140 150
VFLCDMSVFD AYMEPGPRRQ SSLFQWENSR ALCSAPACDI IPQDEIIPRA
160 170 180 190 200
HCRLLCSQQP FEVVEKACRS YSHVVLKEVR FFNLQSLYPL LKDPSLNLHI
210 220 230 240 250
VHLVRDPRAV FRSRERTKGD LMIDSRIVMG QHEQKLKKED QPYYVMQVIC
260 270 280 290 300
QSQLEIYKTI QSLPKALQER YLLVRYEDLA RAPVAQTSRM YEFVGLEFLP
310 320 330 340 350
HLQTWVHNIT RGKGMGDHAF HTNARDALNV SQAWRWSLPY EKVSRLQKAC
360 370 380
GDAMNLLGYR HVRSEQEQRN LLLDLLSTWT VPEQIH
Length:386
Mass (Da):45,134
Last modified:March 15, 2005 - v2
Checksum:i0C3BB4022417143A
GO

Sequence cautioni

The sequence AAH35282 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti225S → N in BAC11177 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052528361H → Q. Corresponds to variant dbSNP:rs3813744Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF131235 mRNA. Translation: AAD33015.1.
AF149783 mRNA. Translation: AAK48417.1.
AF280088 mRNA. Translation: AAG48246.1.
AK074746 mRNA. Translation: BAC11177.1.
BC035282 mRNA. Translation: AAH35282.1. Different initiation.
CCDSiCCDS10902.1.
RefSeqiNP_001159867.1. NM_001166395.1.
NP_005760.1. NM_005769.2.
UniGeneiHs.251383.

Genome annotation databases

EnsembliENST00000338482; ENSP00000341206; ENSG00000140835.
ENST00000539698; ENSP00000441204; ENSG00000140835.
GeneIDi10164.
KEGGihsa:10164.
UCSCiuc002fan.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCHST4_HUMAN
AccessioniPrimary (citable) accession number: Q8NCG5
Secondary accession number(s): Q8IV46, Q9Y5R3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: September 27, 2017
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families