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Q8NCG5

- CHST4_HUMAN

UniProt

Q8NCG5 - CHST4_HUMAN

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Protein

Carbohydrate sulfotransferase 4

Gene

CHST4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi51 – 577PAPSBy similarity
Nucleotide bindingi205 – 2139PAPSBy similarity

GO - Molecular functioni

  1. N-acetylglucosamine 6-O-sulfotransferase activity Source: UniProtKB
  2. sulfotransferase activity Source: ProtInc

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. cell adhesion Source: ProtInc
  3. cell-cell signaling Source: ProtInc
  4. cellular component movement Source: ProtInc
  5. immune response Source: ProtInc
  6. inflammatory response Source: UniProtKB-KW
  7. leukocyte tethering or rolling Source: Ensembl
  8. N-acetylglucosamine metabolic process Source: UniProtKB
  9. protein sulfation Source: ProtInc
  10. sulfur compound metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism, Inflammatory response

Enzyme and pathway databases

SABIO-RKQ8NCG5.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 4 (EC:2.8.2.-)
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3
Short name:
GST-3
High endothelial cells N-acetylglucosamine 6-O-sulfotransferase
Short name:
HEC-GlcNAc6ST
L-selectin ligand sulfotransferase
Short name:
LSST
N-acetylglucosamine 6-O-sulfotransferase 2
Short name:
GlcNAc6ST-2
Short name:
Gn6st-2
Gene namesi
Name:CHST4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:1972. CHST4.

Subcellular locationi

Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein 1 Publication

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. intrinsic component of Golgi membrane Source: UniProtKB
  3. trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26504.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Carbohydrate sulfotransferase 4PRO_0000085193Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi30 – 301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi308 – 3081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8NCG5.
PRIDEiQ8NCG5.

PTM databases

PhosphoSiteiQ8NCG5.

Expressioni

Tissue specificityi

Specifically expressed in HEV. Weakly expressed in spleen. Not expressed in other tissues. Expressed in colonic mucinous adenocarcinoma.3 Publications

Inductioni

Upon cytokine activation, it is expressed at low level.1 Publication

Gene expression databases

BgeeiQ8NCG5.
CleanExiHS_CHST4.
GenevestigatoriQ8NCG5.

Organism-specific databases

HPAiHPA021955.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi115466. 1 interaction.
IntActiQ8NCG5. 1 interaction.
STRINGi9606.ENSP00000341206.

Structurei

3D structure databases

ProteinModelPortaliQ8NCG5.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Topological domaini29 – 386358LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG80862.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG050949.
InParanoidiQ8NCG5.
KOiK04746.
OMAiFHTNARN.
OrthoDBiEOG7RZ5S0.
PhylomeDBiQ8NCG5.
TreeFamiTF342871.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8NCG5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLPKKMKLL LFLVSQMAIL ALFFHMYSHN ISSLSMKAQP ERMHVLVLSS
60 70 80 90 100
WRSGSSFVGQ LFGQHPDVFY LMEPAWHVWM TFKQSTAWML HMAVRDLIRA
110 120 130 140 150
VFLCDMSVFD AYMEPGPRRQ SSLFQWENSR ALCSAPACDI IPQDEIIPRA
160 170 180 190 200
HCRLLCSQQP FEVVEKACRS YSHVVLKEVR FFNLQSLYPL LKDPSLNLHI
210 220 230 240 250
VHLVRDPRAV FRSRERTKGD LMIDSRIVMG QHEQKLKKED QPYYVMQVIC
260 270 280 290 300
QSQLEIYKTI QSLPKALQER YLLVRYEDLA RAPVAQTSRM YEFVGLEFLP
310 320 330 340 350
HLQTWVHNIT RGKGMGDHAF HTNARDALNV SQAWRWSLPY EKVSRLQKAC
360 370 380
GDAMNLLGYR HVRSEQEQRN LLLDLLSTWT VPEQIH
Length:386
Mass (Da):45,134
Last modified:March 15, 2005 - v2
Checksum:i0C3BB4022417143A
GO

Sequence cautioni

The sequence AAH35282.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti225 – 2251S → N in BAC11177. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti361 – 3611H → Q.
Corresponds to variant rs3813744 [ dbSNP | Ensembl ].
VAR_052528

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF131235 mRNA. Translation: AAD33015.1.
AF149783 mRNA. Translation: AAK48417.1.
AF280088 mRNA. Translation: AAG48246.1.
AK074746 mRNA. Translation: BAC11177.1.
BC035282 mRNA. Translation: AAH35282.1. Different initiation.
CCDSiCCDS10902.1.
RefSeqiNP_001159867.1. NM_001166395.1.
NP_005760.1. NM_005769.2.
UniGeneiHs.251383.

Genome annotation databases

EnsembliENST00000338482; ENSP00000341206; ENSG00000140835.
ENST00000539698; ENSP00000441204; ENSG00000140835.
GeneIDi10164.
KEGGihsa:10164.
UCSCiuc002fan.3. human.

Polymorphism databases

DMDMi61211834.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF131235 mRNA. Translation: AAD33015.1 .
AF149783 mRNA. Translation: AAK48417.1 .
AF280088 mRNA. Translation: AAG48246.1 .
AK074746 mRNA. Translation: BAC11177.1 .
BC035282 mRNA. Translation: AAH35282.1 . Different initiation.
CCDSi CCDS10902.1.
RefSeqi NP_001159867.1. NM_001166395.1.
NP_005760.1. NM_005769.2.
UniGenei Hs.251383.

3D structure databases

ProteinModelPortali Q8NCG5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115466. 1 interaction.
IntActi Q8NCG5. 1 interaction.
STRINGi 9606.ENSP00000341206.

Chemistry

ChEMBLi CHEMBL2239.

PTM databases

PhosphoSitei Q8NCG5.

Polymorphism databases

DMDMi 61211834.

Proteomic databases

PaxDbi Q8NCG5.
PRIDEi Q8NCG5.

Protocols and materials databases

DNASUi 10164.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000338482 ; ENSP00000341206 ; ENSG00000140835 .
ENST00000539698 ; ENSP00000441204 ; ENSG00000140835 .
GeneIDi 10164.
KEGGi hsa:10164.
UCSCi uc002fan.3. human.

Organism-specific databases

CTDi 10164.
GeneCardsi GC16P071560.
HGNCi HGNC:1972. CHST4.
HPAi HPA021955.
neXtProti NX_Q8NCG5.
PharmGKBi PA26504.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG80862.
GeneTreei ENSGT00530000062902.
HOGENOMi HOG000261614.
HOVERGENi HBG050949.
InParanoidi Q8NCG5.
KOi K04746.
OMAi FHTNARN.
OrthoDBi EOG7RZ5S0.
PhylomeDBi Q8NCG5.
TreeFami TF342871.

Enzyme and pathway databases

SABIO-RK Q8NCG5.

Miscellaneous databases

GeneWikii CHST4.
GenomeRNAii 10164.
NextBioi 38482.
PROi Q8NCG5.

Gene expression databases

Bgeei Q8NCG5.
CleanExi HS_CHST4.
Genevestigatori Q8NCG5.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view ]
Pfami PF00685. Sulfotransfer_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMi SSF52540. SSF52540. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for L-selectin."
    Bistrup A., Bhakta S., Lee J.K., Belov Y.Y., Gunn M.D., Zuo F.-R., Huang C.-C., Kannagi R., Rosen S.D., Hemmerich S.
    J. Cell Biol. 145:899-910(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Tonsil.
  2. "Novel sulfated lymphocyte homing receptors and their control by a Core1 extension beta 1,3-N-acetylglucosaminyltransferase."
    Yeh J.-C., Hiraoka N., Petryniak B., Nakayama J., Ellies L.G., Rabuka D., Hindsgaul O., Marth J.D., Lowe J.B., Fakuda M.
    Cell 105:957-969(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Chromosomal localization and genomic organization for the galactose/ N-acetylgalactosamine/N-acetylglucosamine 6-O-sulfotransferase gene family."
    Hemmerich S., Lee J.K., Bhakta S., Bistrup A., Ruddle N.R., Rosen S.D.
    Glycobiology 11:75-87(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Tonsil.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Teratocarcinoma.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "CHST1 and CHST2 sulfotransferase expression by vascular endothelial cells regulates shear-resistant leukocyte rolling via L-selectin."
    Li X., Tu L., Murphy P.G., Kadono T., Steeber D.A., Tedder T.F.
    J. Leukoc. Biol. 69:565-574(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  7. "Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic mucinous adenocarcinoma."
    Seko A., Nagata K., Yonezawa S., Yamashita K.
    Glycobiology 12:379-388(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Specificities of N-acetylglucosamine-6-O-sulfotransferases in relation to L-selectin ligand synthesis and tumor-associated enzyme expression."
    Uchimura K., El-Fasakhany F.M., Hori M., Hemmerich S., Blink S.E., Kansas G.S., Kanamori A., Kumamoto K., Kannagi R., Muramatsu T.
    J. Biol. Chem. 277:3979-3984(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBSTRATE SPECIFICITY.
  9. "Golgi localization of carbohydrate sulfotransferases is a determinant of L-selectin ligand biosynthesis."
    de Graffenried C.L., Bertozzi C.R.
    J. Biol. Chem. 278:40282-40295(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. "The stem region of the sulfotransferase GlcNAc6ST-1 is a determinant of substrate specificity."
    de Graffenried C.L., Bertozzi C.R.
    J. Biol. Chem. 279:40035-40043(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiCHST4_HUMAN
AccessioniPrimary (citable) accession number: Q8NCG5
Secondary accession number(s): Q8IV46, Q9Y5R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: October 29, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

May serve as a antiinflammatory target.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3