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Q8NCG5

- CHST4_HUMAN

UniProt

Q8NCG5 - CHST4_HUMAN

Protein

Carbohydrate sulfotransferase 4

Gene

CHST4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi51 – 577PAPSBy similarity
    Nucleotide bindingi205 – 2139PAPSBy similarity

    GO - Molecular functioni

    1. N-acetylglucosamine 6-O-sulfotransferase activity Source: UniProtKB
    2. sulfotransferase activity Source: ProtInc

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW
    2. cell adhesion Source: ProtInc
    3. cell-cell signaling Source: ProtInc
    4. cellular component movement Source: ProtInc
    5. immune response Source: ProtInc
    6. inflammatory response Source: UniProtKB-KW
    7. leukocyte tethering or rolling Source: Ensembl
    8. N-acetylglucosamine metabolic process Source: UniProtKB
    9. protein sulfation Source: ProtInc
    10. sulfur compound metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Inflammatory response

    Enzyme and pathway databases

    SABIO-RKQ8NCG5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbohydrate sulfotransferase 4 (EC:2.8.2.-)
    Alternative name(s):
    Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3
    Short name:
    GST-3
    High endothelial cells N-acetylglucosamine 6-O-sulfotransferase
    Short name:
    HEC-GlcNAc6ST
    L-selectin ligand sulfotransferase
    Short name:
    LSST
    N-acetylglucosamine 6-O-sulfotransferase 2
    Short name:
    GlcNAc6ST-2
    Short name:
    Gn6st-2
    Gene namesi
    Name:CHST4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:1972. CHST4.

    Subcellular locationi

    Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. intrinsic component of Golgi membrane Source: UniProtKB
    3. trans-Golgi network Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26504.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 386386Carbohydrate sulfotransferase 4PRO_0000085193Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi30 – 301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi308 – 3081N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8NCG5.
    PRIDEiQ8NCG5.

    PTM databases

    PhosphoSiteiQ8NCG5.

    Expressioni

    Tissue specificityi

    Specifically expressed in HEV. Weakly expressed in spleen. Not expressed in other tissues. Expressed in colonic mucinous adenocarcinoma.3 Publications

    Inductioni

    Upon cytokine activation, it is expressed at low level.1 Publication

    Gene expression databases

    BgeeiQ8NCG5.
    CleanExiHS_CHST4.
    GenevestigatoriQ8NCG5.

    Organism-specific databases

    HPAiHPA021955.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi115466. 1 interaction.
    IntActiQ8NCG5. 1 interaction.
    STRINGi9606.ENSP00000341206.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NCG5.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 77CytoplasmicSequence Analysis
    Topological domaini29 – 386358LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG80862.
    HOGENOMiHOG000261614.
    HOVERGENiHBG050949.
    InParanoidiQ8NCG5.
    KOiK04746.
    OMAiFHTNARN.
    OrthoDBiEOG7RZ5S0.
    PhylomeDBiQ8NCG5.
    TreeFamiTF342871.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR016469. Carbohydrate_sulfotransferase.
    IPR027417. P-loop_NTPase.
    IPR000863. Sulfotransferase_dom.
    [Graphical view]
    PfamiPF00685. Sulfotransfer_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
    SUPFAMiSSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q8NCG5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLPKKMKLL LFLVSQMAIL ALFFHMYSHN ISSLSMKAQP ERMHVLVLSS    50
    WRSGSSFVGQ LFGQHPDVFY LMEPAWHVWM TFKQSTAWML HMAVRDLIRA 100
    VFLCDMSVFD AYMEPGPRRQ SSLFQWENSR ALCSAPACDI IPQDEIIPRA 150
    HCRLLCSQQP FEVVEKACRS YSHVVLKEVR FFNLQSLYPL LKDPSLNLHI 200
    VHLVRDPRAV FRSRERTKGD LMIDSRIVMG QHEQKLKKED QPYYVMQVIC 250
    QSQLEIYKTI QSLPKALQER YLLVRYEDLA RAPVAQTSRM YEFVGLEFLP 300
    HLQTWVHNIT RGKGMGDHAF HTNARDALNV SQAWRWSLPY EKVSRLQKAC 350
    GDAMNLLGYR HVRSEQEQRN LLLDLLSTWT VPEQIH 386
    Length:386
    Mass (Da):45,134
    Last modified:March 15, 2005 - v2
    Checksum:i0C3BB4022417143A
    GO

    Sequence cautioni

    The sequence AAH35282.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti225 – 2251S → N in BAC11177. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti361 – 3611H → Q.
    Corresponds to variant rs3813744 [ dbSNP | Ensembl ].
    VAR_052528

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF131235 mRNA. Translation: AAD33015.1.
    AF149783 mRNA. Translation: AAK48417.1.
    AF280088 mRNA. Translation: AAG48246.1.
    AK074746 mRNA. Translation: BAC11177.1.
    BC035282 mRNA. Translation: AAH35282.1. Different initiation.
    CCDSiCCDS10902.1.
    RefSeqiNP_001159867.1. NM_001166395.1.
    NP_005760.1. NM_005769.2.
    UniGeneiHs.251383.

    Genome annotation databases

    EnsembliENST00000338482; ENSP00000341206; ENSG00000140835.
    ENST00000539698; ENSP00000441204; ENSG00000140835.
    GeneIDi10164.
    KEGGihsa:10164.
    UCSCiuc002fan.3. human.

    Polymorphism databases

    DMDMi61211834.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF131235 mRNA. Translation: AAD33015.1 .
    AF149783 mRNA. Translation: AAK48417.1 .
    AF280088 mRNA. Translation: AAG48246.1 .
    AK074746 mRNA. Translation: BAC11177.1 .
    BC035282 mRNA. Translation: AAH35282.1 . Different initiation.
    CCDSi CCDS10902.1.
    RefSeqi NP_001159867.1. NM_001166395.1.
    NP_005760.1. NM_005769.2.
    UniGenei Hs.251383.

    3D structure databases

    ProteinModelPortali Q8NCG5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115466. 1 interaction.
    IntActi Q8NCG5. 1 interaction.
    STRINGi 9606.ENSP00000341206.

    Chemistry

    ChEMBLi CHEMBL2239.

    PTM databases

    PhosphoSitei Q8NCG5.

    Polymorphism databases

    DMDMi 61211834.

    Proteomic databases

    PaxDbi Q8NCG5.
    PRIDEi Q8NCG5.

    Protocols and materials databases

    DNASUi 10164.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000338482 ; ENSP00000341206 ; ENSG00000140835 .
    ENST00000539698 ; ENSP00000441204 ; ENSG00000140835 .
    GeneIDi 10164.
    KEGGi hsa:10164.
    UCSCi uc002fan.3. human.

    Organism-specific databases

    CTDi 10164.
    GeneCardsi GC16P071560.
    HGNCi HGNC:1972. CHST4.
    HPAi HPA021955.
    neXtProti NX_Q8NCG5.
    PharmGKBi PA26504.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG80862.
    HOGENOMi HOG000261614.
    HOVERGENi HBG050949.
    InParanoidi Q8NCG5.
    KOi K04746.
    OMAi FHTNARN.
    OrthoDBi EOG7RZ5S0.
    PhylomeDBi Q8NCG5.
    TreeFami TF342871.

    Enzyme and pathway databases

    SABIO-RK Q8NCG5.

    Miscellaneous databases

    GeneWikii CHST4.
    GenomeRNAii 10164.
    NextBioi 38482.
    PROi Q8NCG5.

    Gene expression databases

    Bgeei Q8NCG5.
    CleanExi HS_CHST4.
    Genevestigatori Q8NCG5.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR016469. Carbohydrate_sulfotransferase.
    IPR027417. P-loop_NTPase.
    IPR000863. Sulfotransferase_dom.
    [Graphical view ]
    Pfami PF00685. Sulfotransfer_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
    SUPFAMi SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for L-selectin."
      Bistrup A., Bhakta S., Lee J.K., Belov Y.Y., Gunn M.D., Zuo F.-R., Huang C.-C., Kannagi R., Rosen S.D., Hemmerich S.
      J. Cell Biol. 145:899-910(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
      Tissue: Tonsil.
    2. "Novel sulfated lymphocyte homing receptors and their control by a Core1 extension beta 1,3-N-acetylglucosaminyltransferase."
      Yeh J.-C., Hiraoka N., Petryniak B., Nakayama J., Ellies L.G., Rabuka D., Hindsgaul O., Marth J.D., Lowe J.B., Fakuda M.
      Cell 105:957-969(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Chromosomal localization and genomic organization for the galactose/ N-acetylgalactosamine/N-acetylglucosamine 6-O-sulfotransferase gene family."
      Hemmerich S., Lee J.K., Bhakta S., Bistrup A., Ruddle N.R., Rosen S.D.
      Glycobiology 11:75-87(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Tonsil.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Teratocarcinoma.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. "CHST1 and CHST2 sulfotransferase expression by vascular endothelial cells regulates shear-resistant leukocyte rolling via L-selectin."
      Li X., Tu L., Murphy P.G., Kadono T., Steeber D.A., Tedder T.F.
      J. Leukoc. Biol. 69:565-574(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.
    7. "Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic mucinous adenocarcinoma."
      Seko A., Nagata K., Yonezawa S., Yamashita K.
      Glycobiology 12:379-388(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    8. "Specificities of N-acetylglucosamine-6-O-sulfotransferases in relation to L-selectin ligand synthesis and tumor-associated enzyme expression."
      Uchimura K., El-Fasakhany F.M., Hori M., Hemmerich S., Blink S.E., Kansas G.S., Kanamori A., Kumamoto K., Kannagi R., Muramatsu T.
      J. Biol. Chem. 277:3979-3984(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBSTRATE SPECIFICITY.
    9. "Golgi localization of carbohydrate sulfotransferases is a determinant of L-selectin ligand biosynthesis."
      de Graffenried C.L., Bertozzi C.R.
      J. Biol. Chem. 278:40282-40295(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    10. "The stem region of the sulfotransferase GlcNAc6ST-1 is a determinant of substrate specificity."
      de Graffenried C.L., Bertozzi C.R.
      J. Biol. Chem. 279:40035-40043(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.

    Entry informationi

    Entry nameiCHST4_HUMAN
    AccessioniPrimary (citable) accession number: Q8NCG5
    Secondary accession number(s): Q8IV46, Q9Y5R3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    May serve as a antiinflammatory target.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3