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Protein

NFATC2-interacting protein

Gene

NFATC2IP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

In T-helper 2 (Th2) cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2. Recruits PRMT1 to the IL4 promoter; this leads to enhancement of histone H4 'Arg-3'-methylation and facilitates subsequent histone acetylation at the IL4 locus, thus promotes robust cytokine expression (By similarity). Down-regulates formation of poly-SUMO chains by UBE2I/UBC9 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000176953-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NFATC2-interacting protein
Alternative name(s):
45 kDa NF-AT-interacting protein
Short name:
45 kDa NFAT-interacting protein
Nuclear factor of activated T-cells, cytoplasmic 2-interacting protein
Gene namesi
Name:NFATC2IP
Synonyms:NIP45
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:25906. NFATC2IP.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: TRAF1 is associated with a fraction of NFATC2IP in the cytoplasm and prevents its translocation to the nucleus.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000176953.
PharmGKBiPA134887712.

Polymorphism and mutation databases

BioMutaiNFATC2IP.
DMDMi74751188.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002810081 – 419NFATC2-interacting proteinAdd BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54PhosphoserineBy similarity1
Modified residuei84PhosphoserineCombined sources1
Modified residuei88PhosphoserineBy similarity1
Modified residuei90PhosphoserineBy similarity1
Modified residuei92PhosphoserineBy similarity1
Modified residuei127PhosphoserineCombined sources1
Modified residuei198PhosphoserineCombined sources1
Modified residuei201PhosphoserineCombined sources1
Modified residuei204PhosphoserineCombined sources1
Modified residuei220PhosphoserineCombined sources1
Modified residuei314PhosphoserineCombined sources1
Modified residuei316PhosphothreonineCombined sources1
Modified residuei318PhosphothreonineCombined sources1
Modified residuei369PhosphoserineCombined sources1
Modified residuei390PhosphoserineCombined sources1

Post-translational modificationi

Methylation at the N-terminus by PRMT1 modulates interaction with the NFAT complex and results in augmented cytokine production.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ8NCF5.
MaxQBiQ8NCF5.
PaxDbiQ8NCF5.
PeptideAtlasiQ8NCF5.
PRIDEiQ8NCF5.
TopDownProteomicsiQ8NCF5-2. [Q8NCF5-2]

PTM databases

iPTMnetiQ8NCF5.
PhosphoSitePlusiQ8NCF5.

Expressioni

Gene expression databases

BgeeiENSG00000176953.
CleanExiHS_NFATC2IP.
ExpressionAtlasiQ8NCF5. baseline and differential.
GenevisibleiQ8NCF5. HS.

Organism-specific databases

HPAiHPA053587.

Interactioni

Subunit structurei

Interacts with NFATC2, TRAF1, TRAF2 and PRMT1. Interacts with UBE2I/UBC9 (By similarity).By similarity

Protein-protein interaction databases

BioGridi124341. 32 interactors.
IntActiQ8NCF5. 4 interactors.
MINTiMINT-6169168.
STRINGi9606.ENSP00000324792.

Structurei

Secondary structure

1419
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi252 – 254Combined sources3
Beta strandi265 – 270Combined sources6
Beta strandi272 – 280Combined sources9
Beta strandi282 – 284Combined sources3
Helixi287 – 297Combined sources11
Helixi301 – 303Combined sources3
Beta strandi304 – 308Combined sources5
Helixi319 – 322Combined sources4
Beta strandi329 – 334Combined sources6
Beta strandi347 – 353Combined sources7
Beta strandi359 – 366Combined sources8
Helixi371 – 382Combined sources12
Beta strandi385 – 387Combined sources3
Beta strandi390 – 393Combined sources4
Helixi404 – 407Combined sources4
Beta strandi414 – 418Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JXXNMR-A342-419[»]
2L76NMR-A244-338[»]
3RD2X-ray1.60A345-419[»]
ProteinModelPortaliQ8NCF5.
SMRiQ8NCF5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8NCF5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini348 – 419Ubiquitin-likePROSITE-ProRule annotationAdd BLAST72

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili209 – 231Sequence analysisAdd BLAST23

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi108 – 112Poly-Arg5

Sequence similaritiesi

Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1769. Eukaryota.
COG5227. LUCA.
GeneTreeiENSGT00390000007119.
HOGENOMiHOG000285948.
HOVERGENiHBG082020.
InParanoidiQ8NCF5.
OMAiSHYEEAM.
OrthoDBiEOG091G0KS7.
PhylomeDBiQ8NCF5.
TreeFamiTF328600.

Family and domain databases

InterProiIPR022617. Rad60/SUMO-like_dom.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF11976. Rad60-SLD. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 2 hits.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 2 hits.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NCF5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEPVGKRGR WSGGSGAGRG GRGGWGGRGR RPRAQRSPSR GTLDVVSVDL
60 70 80 90 100
VTDSDEEILE VATARGAADE VEVEPPEPPG PVASRDNSNS DSEGEDRRPA
110 120 130 140 150
GPPREPVRRR RRLVLDPGEA PLVPVYSGKV KSSLRLIPDD LSLLKLYPPG
160 170 180 190 200
DEEEAELADS SGLYHEGSPS PGSPWKTKLR TKDKEEKKKT EFLDLDNSPL
210 220 230 240 250
SPPSPRTKSR THTRALKKLS EVNKRLQDLR SCLSPKPPQG QEQQGQEDEV
260 270 280 290 300
VLVEGPTLPE TPRLFPLKIR CRADLVRLPL RMSEPLQSVV DHMATHLGVS
310 320 330 340 350
PSRILLLFGE TELSPTATPR TLKLGVADII DCVVLTSSPE ATETSQQLQL
360 370 380 390 400
RVQGKEKHQT LEVSLSRDSP LKTLMSHYEE AMGLSGRKLS FFFDGTKLSG
410
RELPADLGME SGDLIEVWG
Length:419
Mass (Da):45,817
Last modified:October 1, 2002 - v1
Checksum:iCA04220B4BF0B245
GO
Isoform 2 (identifier: Q8NCF5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-281: Missing.

Note: No experimental confirmation available.
Show »
Length:138
Mass (Da):15,054
Checksum:i88093D025BC0865D
GO
Isoform 3 (identifier: Q8NCF5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-292: Missing.

Show »
Length:127
Mass (Da):13,831
Checksum:iBE391D16B4ED93BF
GO

Sequence cautioni

The sequence AAM49721 differs from that shown. Reason: Frameshift at positions 107 and 114.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03120833R → W.Corresponds to variant rs7201257dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0239311 – 292Missing in isoform 3. 2 PublicationsAdd BLAST292
Alternative sequenceiVSP_0239321 – 281Missing in isoform 2. 1 PublicationAdd BLAST281

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027545 mRNA. Translation: BAB55189.1.
AK074761 mRNA. Translation: BAC11189.1.
AK297333 mRNA. Translation: BAH12551.1.
AK316535 mRNA. Translation: BAH14906.1.
AF458593 mRNA. Translation: AAM49721.1. Frameshift.
BC018311 mRNA. Translation: AAH18311.2.
BC021551 mRNA. Translation: AAH21551.2.
BC068007 mRNA. Translation: AAH68007.1.
BC080628 mRNA. Translation: AAH80628.1.
BC101741 mRNA. Translation: AAI01742.1.
BC112182 mRNA. Translation: AAI12183.1.
CCDSiCCDS10645.1. [Q8NCF5-1]
RefSeqiNP_116204.3. NM_032815.3. [Q8NCF5-1]
UniGeneiHs.513470.

Genome annotation databases

EnsembliENST00000320805; ENSP00000324792; ENSG00000176953. [Q8NCF5-1]
ENST00000568148; ENSP00000454958; ENSG00000176953. [Q8NCF5-3]
GeneIDi84901.
KEGGihsa:84901.
UCSCiuc002dru.4. human. [Q8NCF5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027545 mRNA. Translation: BAB55189.1.
AK074761 mRNA. Translation: BAC11189.1.
AK297333 mRNA. Translation: BAH12551.1.
AK316535 mRNA. Translation: BAH14906.1.
AF458593 mRNA. Translation: AAM49721.1. Frameshift.
BC018311 mRNA. Translation: AAH18311.2.
BC021551 mRNA. Translation: AAH21551.2.
BC068007 mRNA. Translation: AAH68007.1.
BC080628 mRNA. Translation: AAH80628.1.
BC101741 mRNA. Translation: AAI01742.1.
BC112182 mRNA. Translation: AAI12183.1.
CCDSiCCDS10645.1. [Q8NCF5-1]
RefSeqiNP_116204.3. NM_032815.3. [Q8NCF5-1]
UniGeneiHs.513470.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JXXNMR-A342-419[»]
2L76NMR-A244-338[»]
3RD2X-ray1.60A345-419[»]
ProteinModelPortaliQ8NCF5.
SMRiQ8NCF5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124341. 32 interactors.
IntActiQ8NCF5. 4 interactors.
MINTiMINT-6169168.
STRINGi9606.ENSP00000324792.

PTM databases

iPTMnetiQ8NCF5.
PhosphoSitePlusiQ8NCF5.

Polymorphism and mutation databases

BioMutaiNFATC2IP.
DMDMi74751188.

Proteomic databases

EPDiQ8NCF5.
MaxQBiQ8NCF5.
PaxDbiQ8NCF5.
PeptideAtlasiQ8NCF5.
PRIDEiQ8NCF5.
TopDownProteomicsiQ8NCF5-2. [Q8NCF5-2]

Protocols and materials databases

DNASUi84901.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000320805; ENSP00000324792; ENSG00000176953. [Q8NCF5-1]
ENST00000568148; ENSP00000454958; ENSG00000176953. [Q8NCF5-3]
GeneIDi84901.
KEGGihsa:84901.
UCSCiuc002dru.4. human. [Q8NCF5-1]

Organism-specific databases

CTDi84901.
GeneCardsiNFATC2IP.
H-InvDBHIX0018982.
HGNCiHGNC:25906. NFATC2IP.
HPAiHPA053587.
MIMi614525. gene.
neXtProtiNX_Q8NCF5.
OpenTargetsiENSG00000176953.
PharmGKBiPA134887712.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1769. Eukaryota.
COG5227. LUCA.
GeneTreeiENSGT00390000007119.
HOGENOMiHOG000285948.
HOVERGENiHBG082020.
InParanoidiQ8NCF5.
OMAiSHYEEAM.
OrthoDBiEOG091G0KS7.
PhylomeDBiQ8NCF5.
TreeFamiTF328600.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000176953-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ8NCF5.
GenomeRNAii84901.
PROiQ8NCF5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000176953.
CleanExiHS_NFATC2IP.
ExpressionAtlasiQ8NCF5. baseline and differential.
GenevisibleiQ8NCF5. HS.

Family and domain databases

InterProiIPR022617. Rad60/SUMO-like_dom.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF11976. Rad60-SLD. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 2 hits.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 2 hits.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNF2IP_HUMAN
AccessioniPrimary (citable) accession number: Q8NCF5
Secondary accession number(s): B7Z4G5
, Q66K34, Q6NVK1, Q8NFR2, Q96ST9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.