Q8NC96 (NECP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adaptin ear-binding coat-associated protein 1

Alternative name(s):
NECAP endocytosis-associated protein 1
Short name=NECAP-1

Gene names

Name:

NECAP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in endocytosis By similarity.
Subunit structure	Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3 By similarity.
Subcellular location	Cytoplasmic vesicle › clathrin-coated vesicle membrane By similarity. Cell membrane By similarity. Note: Colocalizes with AP-2 at the plasma membrane By similarity.
Domain	The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself. The WXXF motif 1, which is preceded by an acidic residue and has a glycine in second position mediates specific interaction with AP-1. The WXXF motif 2, which is followed by the C-terminal carboxyl group negative charge, allows specific interaction with AP-2 By similarity.
Sequence similarities	Belongs to the NECAP family.
Sequence caution	The sequence AAH02888.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Endocytosis Protein transport Transport
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	clathrin coated vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NC96-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NC96-2)

The sequence of this isoform differs from the canonical sequence as follows:
     102-102: R → G
     103-275: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Adaptin ear-binding coat-associated protein 1		PRO_0000213067
Regions
	Motif	252 – 255	4	WXXF motif 1
	Motif	272 – 275	4	WXXF motif 2
Amino acid modifications
	Modified residue	207	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	102	1	R → G in isoform 2.		VSP_013232
	Alternative sequence	103 – 275	173	Missing in isoform 2.		VSP_013233
	Natural variant	224	1	D → N. Corresponds to variant rs2231752 [ dbSNP | Ensembl ].		VAR_034153
Experimental info
	Sequence conflict	77	1	Y → F in CAB43373. Ref.2
	Sequence conflict	232	1	D → G in BAC11250. Ref.1
	Sequence conflict	239	1	T → A in BAC11264. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 22FC4CCEC7E3B713	FASTA	275	29,737
	10 20 30 40 50 60 MATELEYESV LCVKPDVSVY RIPPRASNRG YRASDWKLDQ PDWTGRLRIT SKGKTAYIKL 70 80 90 100 110 120 EDKVSGELFA QAPVEQYPGI AVETVTDSSR YFVIRIQDGT GRSAFIGIGF TDRGDAFDFN 130 140 150 160 170 180 VSLQDHFKWV KQESEISKES QEMDARPKLD LGFKEGQTIK LCIGNITNKK GGASKPRTAR 190 200 210 220 230 240 GGGLSLLPPP PGGKVTIPPP SSSVAISNHV TPPPIPKSNH GGSDADILLD LDSPAPVTTP 250 260 270 APTPVSVSND LWGDFSTASS SVPNQAPQPS NWVQF
	Isoform 2 [UniParc]. Checksum: A88F613B5A5DEEF3	FASTA	102	11,444
	10 20 30 40 50 60 MATELEYESV LCVKPDVSVY RIPPRASNRG YRASDWKLDQ PDWTGRLRIT SKGKTAYIKL 70 80 90 100 EDKVSGELFA QAPVEQYPGI AVETVTDSSR YFVIRIQDGT GG

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Teratocarcinoma.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Kidney.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain, Lung, Lymph and Muscle.
[4]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK074858 mRNA. Translation: BAC11250.1. AK074880 mRNA. Translation: BAC11264.1. AK074923 mRNA. Translation: BAC11296.1. AK075013 mRNA. Translation: BAC11352.1. AL050272 mRNA. Translation: CAB43373.2. BC002888 mRNA. Translation: AAH02888.1. Different initiation. BC067367 mRNA. Translation: AAH67367.1. BC084551 mRNA. Translation: AAH84551.1. BC110876 mRNA. Translation: AAI10877.1.
IPI	IPI00170916. IPI01025934.
PIR	T08719.
RefSeq	NP_056324.2. NM_015509.3.
UniGene	Hs.555927.
3D structure databases
ProteinModelPortal	Q8NC96.
SMR	Q8NC96. Positions 1-133.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8NC96. 1 interaction.
STRING	Q8NC96.
PTM databases
PhosphoSite	Q8NC96.
Polymorphism databases
DMDM	62287155.
Proteomic databases
PRIDE	Q8NC96.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000339754; ENSP00000341737; ENSG00000089818.
GeneID	25977.
KEGG	hsa:25977.
UCSC	uc001qtx.1. human.
Organism-specific databases
CTD	25977.
GeneCards	GC12P007926.
H-InvDB	HIX0010404.
HGNC	HGNC:24539. NECAP1.
MIM	611623. gene.
neXtProt	NX_Q8NC96.
PharmGKB	PA142671267.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15818.
GeneTree	ENSGT00390000009359.
HOVERGEN	HBG060621.
InParanoid	Q8NC96.
OMA	ESQEMDS.
PhylomeDB	Q8NC96.
Enzyme and pathway databases
Reactome	REACT_11123. Membrane Trafficking.
Gene expression databases
ArrayExpress	Q8NC96.
Bgee	Q8NC96.
CleanEx	HS_NECAP1.
Genevestigator	Q8NC96.
GermOnline	ENSG00000089818. Homo sapiens.
Family and domain databases
InterPro	IPR012466. NECAP-1. [Graphical view]
Pfam	PF07933. DUF1681. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	47626.
SOURCE	Search...

Entry information

Entry name

NECP1_HUMAN

Accession

Primary (citable) accession number: Q8NC96
Secondary accession number(s): Q2NL73 , Q5XG95, Q6NWY6, Q8N153, Q8NCB0, Q9BU52, Q9Y407

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	January 25, 2012
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.