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Protein

LEM domain-containing protein 2

Gene

LEMD2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in nuclear structure organization (PubMed:16339967). Required for maintaining the integrity of the nuclear envelope (PubMed:17097643).2 Publications
Required for embryonic development and is involved in regulation of several signaling pathways such as MAPK and AKT. Required for myoblast differentiation involving regulation of ERK signaling (By similarity).By similarity

GO - Biological processi

  • heart formation Source: Ensembl
  • negative regulation of MAPK cascade Source: Ensembl
  • negative regulation of protein kinase B signaling Source: Ensembl
  • neurogenesis Source: Ensembl
  • nuclear envelope organization Source: UniProtKB
  • skeletal muscle cell differentiation Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-2993913. Clearance of Nuclear Envelope Membranes from Chromatin.
R-HSA-2995383. Initiation of Nuclear Envelope Reformation.
R-HSA-4419969. Depolymerisation of the Nuclear Lamina.

Names & Taxonomyi

Protein namesi
Recommended name:
LEM domain-containing protein 2
Short name:
hLEM2
Gene namesi
Name:LEMD2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:21244. LEMD2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei213 – 23321HelicalSequence analysisAdd
BLAST
Transmembranei377 – 39721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of nuclear inner membrane Source: MGI
  • membrane Source: UniProtKB
  • nuclear membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134952135.

Polymorphism and mutation databases

BioMutaiLEMD2.
DMDMi74751184.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 503502LEM domain-containing protein 2PRO_0000285249Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Modified residuei166 – 1661PhosphoserineCombined sources
Modified residuei175 – 1751PhosphoserineCombined sources
Modified residuei497 – 4971PhosphoserineCombined sources
Modified residuei499 – 4991PhosphoserineCombined sources
Modified residuei501 – 5011PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8NC56.
MaxQBiQ8NC56.
PaxDbiQ8NC56.
PeptideAtlasiQ8NC56.
PRIDEiQ8NC56.

PTM databases

iPTMnetiQ8NC56.
PhosphoSiteiQ8NC56.
SwissPalmiQ8NC56.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000161904.
CleanExiHS_LEMD2.
ExpressionAtlasiQ8NC56. baseline and differential.
GenevisibleiQ8NC56. HS.

Organism-specific databases

HPAiHPA017340.

Interactioni

Subunit structurei

Binds to the lamin tail, in vitro.1 Publication

Protein-protein interaction databases

BioGridi128735. 36 interactions.
IntActiQ8NC56. 9 interactions.
MINTiMINT-4991274.
STRINGi9606.ENSP00000293760.

Structurei

3D structure databases

ProteinModelPortaliQ8NC56.
SMRiQ8NC56. Positions 398-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 4242LEMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni74 – 10330Interaction with lamin A/C complexesAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi314 – 3174Poly-Ser
Compositional biasi405 – 4084Poly-Glu

Sequence similaritiesi

Contains 1 LEM domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0147. Eukaryota.
ENOG410XP20. LUCA.
GeneTreeiENSGT00530000063791.
HOGENOMiHOG000015214.
HOVERGENiHBG099961.
InParanoidiQ8NC56.
OMAiRAEPWLS.
OrthoDBiEOG091G09QP.
PhylomeDBiQ8NC56.
TreeFamiTF315385.

Family and domain databases

Gene3Di1.10.720.40. 1 hit.
InterProiIPR018996. Inner-Nucl-membr_MAN1.
IPR011015. LEM/LEM-like_dom.
IPR003887. LEM_dom.
[Graphical view]
PfamiPF03020. LEM. 1 hit.
PF09402. MSC. 1 hit.
[Graphical view]
SMARTiSM00540. LEM. 1 hit.
[Graphical view]
SUPFAMiSSF63451. SSF63451. 1 hit.
PROSITEiPS50954. LEM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NC56-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGLSDLELR RELQALGFQP GPITDTTRDV YRNKLRRLRG EARLRDEERL
60 70 80 90 100
REEARPRGEE RLREEARLRE DAPLRARPAA ASPRAEPWLS QPASGSAYAT
110 120 130 140 150
PGAYGDIRPS AASWVGSRGL AYPARPAQLR RRASVRGSSE EDEDARTPDR
160 170 180 190 200
ATQGPGLAAR RWWAASPAPA RLPSSLLGPD PRPGLRATRA GPAGAARARP
210 220 230 240 250
EVGRRLERWL SRLLLWASLG LLLVFLGILW VKMGKPSAPQ EAEDNMKLLP
260 270 280 290 300
VDCERKTDEF CQAKQKAALL ELLHELYNFL AIQAGNFECG NPENLKSKCI
310 320 330 340 350
PVMEAQEYIA NVTSSSSAKF EAALTWILSS NKDVGIWLKG EDQSELVTTV
360 370 380 390 400
DKVVCLESAH PRMGVGCRLS RALLTAVTNV LIFFWCLAFL WGLLILLKYR
410 420 430 440 450
WRKLEEEEQA MYEMVKKIID VVQDHYVDWE QDMERYPYVG ILHVRDSLIP
460 470 480 490 500
PQSRRRMKRV WDRAVEFLAS NESRIQTESH RVAGEDMLVW RWTKPSSFSD

SER
Length:503
Mass (Da):56,975
Last modified:October 1, 2002 - v1
Checksum:i5C24BF18B0423952
GO
Isoform 2 (identifier: Q8NC56-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-302: Missing.

Note: No experimental confirmation available.
Show »
Length:201
Mass (Da):23,520
Checksum:iD5639C76C63E69FD
GO

Sequence cautioni

The sequence CAI21529 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti316 – 3161S → F in BAG62687 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 302302Missing in isoform 2. 1 PublicationVSP_044847Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK301083 mRNA. Translation: BAG62687.1.
AK074959 mRNA. Translation: BAC11316.1.
AL158049 Genomic DNA. Translation: CAI21527.2.
AL158049 Genomic DNA. Translation: CAI21529.1. Sequence problems.
BC051803 mRNA. Translation: AAH51803.1.
CCDSiCCDS47411.1. [Q8NC56-2]
CCDS4785.1. [Q8NC56-1]
RefSeqiNP_001137416.1. NM_001143944.1. [Q8NC56-2]
NP_851853.1. NM_181336.3. [Q8NC56-1]
UniGeneiHs.444845.

Genome annotation databases

EnsembliENST00000293760; ENSP00000293760; ENSG00000161904. [Q8NC56-1]
ENST00000508327; ENSP00000421704; ENSG00000161904. [Q8NC56-2]
ENST00000614475; ENSP00000478539; ENSG00000161904. [Q8NC56-1]
GeneIDi221496.
KEGGihsa:221496.
UCSCiuc011drl.3. human. [Q8NC56-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK301083 mRNA. Translation: BAG62687.1.
AK074959 mRNA. Translation: BAC11316.1.
AL158049 Genomic DNA. Translation: CAI21527.2.
AL158049 Genomic DNA. Translation: CAI21529.1. Sequence problems.
BC051803 mRNA. Translation: AAH51803.1.
CCDSiCCDS47411.1. [Q8NC56-2]
CCDS4785.1. [Q8NC56-1]
RefSeqiNP_001137416.1. NM_001143944.1. [Q8NC56-2]
NP_851853.1. NM_181336.3. [Q8NC56-1]
UniGeneiHs.444845.

3D structure databases

ProteinModelPortaliQ8NC56.
SMRiQ8NC56. Positions 398-500.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128735. 36 interactions.
IntActiQ8NC56. 9 interactions.
MINTiMINT-4991274.
STRINGi9606.ENSP00000293760.

PTM databases

iPTMnetiQ8NC56.
PhosphoSiteiQ8NC56.
SwissPalmiQ8NC56.

Polymorphism and mutation databases

BioMutaiLEMD2.
DMDMi74751184.

Proteomic databases

EPDiQ8NC56.
MaxQBiQ8NC56.
PaxDbiQ8NC56.
PeptideAtlasiQ8NC56.
PRIDEiQ8NC56.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000293760; ENSP00000293760; ENSG00000161904. [Q8NC56-1]
ENST00000508327; ENSP00000421704; ENSG00000161904. [Q8NC56-2]
ENST00000614475; ENSP00000478539; ENSG00000161904. [Q8NC56-1]
GeneIDi221496.
KEGGihsa:221496.
UCSCiuc011drl.3. human. [Q8NC56-1]

Organism-specific databases

CTDi221496.
GeneCardsiLEMD2.
HGNCiHGNC:21244. LEMD2.
HPAiHPA017340.
MIMi616312. gene.
neXtProtiNX_Q8NC56.
PharmGKBiPA134952135.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0147. Eukaryota.
ENOG410XP20. LUCA.
GeneTreeiENSGT00530000063791.
HOGENOMiHOG000015214.
HOVERGENiHBG099961.
InParanoidiQ8NC56.
OMAiRAEPWLS.
OrthoDBiEOG091G09QP.
PhylomeDBiQ8NC56.
TreeFamiTF315385.

Enzyme and pathway databases

ReactomeiR-HSA-2993913. Clearance of Nuclear Envelope Membranes from Chromatin.
R-HSA-2995383. Initiation of Nuclear Envelope Reformation.
R-HSA-4419969. Depolymerisation of the Nuclear Lamina.

Miscellaneous databases

ChiTaRSiLEMD2. human.
GenomeRNAii221496.
PROiQ8NC56.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000161904.
CleanExiHS_LEMD2.
ExpressionAtlasiQ8NC56. baseline and differential.
GenevisibleiQ8NC56. HS.

Family and domain databases

Gene3Di1.10.720.40. 1 hit.
InterProiIPR018996. Inner-Nucl-membr_MAN1.
IPR011015. LEM/LEM-like_dom.
IPR003887. LEM_dom.
[Graphical view]
PfamiPF03020. LEM. 1 hit.
PF09402. MSC. 1 hit.
[Graphical view]
SMARTiSM00540. LEM. 1 hit.
[Graphical view]
SUPFAMiSSF63451. SSF63451. 1 hit.
PROSITEiPS50954. LEM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEMD2_HUMAN
AccessioniPrimary (citable) accession number: Q8NC56
Secondary accession number(s): B4DVH5
, E7EVT2, Q5T972, Q5T974
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.