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Protein

E3 ubiquitin-protein ligase RNF149

Gene

RNF149

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri269 – 310RING-type; atypicalPROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF149 (EC:2.3.2.27)
Alternative name(s):
DNA polymerase-transactivated protein 2
RING finger protein 149
RING-type E3 ubiquitin transferase RNF149Curated
Gene namesi
Name:RNF149
Synonyms:DNAPTP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000163162.8
HGNCiHGNC:23137 RNF149
neXtProtiNX_Q8NC42

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei201 – 221HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000163162
PharmGKBiPA134895641

Polymorphism and mutation databases

BioMutaiRNF149
DMDMi160332298

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000026161133 – 400E3 ubiquitin-protein ligase RNF149Add BLAST368

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi52N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi145N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei345PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8NC42
PaxDbiQ8NC42
PeptideAtlasiQ8NC42
PRIDEiQ8NC42

PTM databases

iPTMnetiQ8NC42
PhosphoSitePlusiQ8NC42

Expressioni

Gene expression databases

BgeeiENSG00000163162
CleanExiHS_RNF149
ExpressionAtlasiQ8NC42 baseline and differential
GenevisibleiQ8NC42 HS

Organism-specific databases

HPAiHPA011424

Interactioni

Protein-protein interaction databases

BioGridi129991, 30 interactors
IntActiQ8NC42, 6 interactors
MINTiQ8NC42
STRINGi9606.ENSP00000295317

Structurei

3D structure databases

ProteinModelPortaliQ8NC42
SMRiQ8NC42
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini67 – 175PAAdd BLAST109

Domaini

The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri269 – 310RING-type; atypicalPROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG0800 Eukaryota
ENOG41121N2 LUCA
GeneTreeiENSGT00760000119057
HOGENOMiHOG000231432
HOVERGENiHBG057659
InParanoidiQ8NC42
KOiK15704
OMAiCAPDTRF
OrthoDBiEOG091G08OR
PhylomeDBiQ8NC42
TreeFamiTF317486

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR003137 PA_domain
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF02225 PA, 1 hit
PF13639 zf-RING_2, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8NC42-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWRRREASV GARGVLALAL LALALCVPGA RGRALEWFSA VVNIEYVDPQ
60 70 80 90 100
TNLTVWSVSE SGRFGDSSPK EGAHGLVGVP WAPGGDLEGC APDTRFFVPE
110 120 130 140 150
PGGRGAAPWV ALVARGGCTF KDKVLVAARR NASAVVLYNE ERYGNITLPM
160 170 180 190 200
SHAGTGNIVV IMISYPKGRE ILELVQKGIP VTMTIGVGTR HVQEFISGQS
210 220 230 240 250
VVFVAIAFIT MMIISLAWLI FYYIQRFLYT GSQIGSQSHR KETKKVIGQL
260 270 280 290 300
LLHTVKHGEK GIDVDAENCA VCIENFKVKD IIRILPCKHI FHRICIDPWL
310 320 330 340 350
LDHRTCPMCK LDVIKALGYW GEPGDVQEMP APESPPGRDP AANLSLALPD
360 370 380 390 400
DDGSDDSSPP SASPAESEPQ CDPSFKGDAG ENTALLEAGR SDSRHGGPIS
Length:400
Mass (Da):43,165
Last modified:November 13, 2007 - v2
Checksum:iDC03309E0E17DCDE
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0359587E → K in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs763258401Ensembl.1
Natural variantiVAR_0294559S → G2 PublicationsCorresponds to variant dbSNP:rs11123868Ensembl.1
Natural variantiVAR_029456344L → F1 PublicationCorresponds to variant dbSNP:rs17856945Ensembl.1
Natural variantiVAR_029457356D → E4 PublicationsCorresponds to variant dbSNP:rs13151Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY450390 mRNA Translation: AAR21083.1
AK074985 mRNA Translation: BAC11334.1
AK075141 mRNA Translation: BAC11430.1
AM392566 mRNA Translation: CAL37444.1
AC013722 Genomic DNA No translation available.
AC073643 Genomic DNA Translation: AAY14775.1
BC019355 mRNA Translation: AAH19355.2
BC032328 mRNA Translation: AAH32328.2
BC045743 mRNA Translation: AAH45743.1
CCDSiCCDS2051.1
RefSeqiNP_775918.2, NM_173647.3
UniGeneiHs.142074

Genome annotation databases

EnsembliENST00000295317; ENSP00000295317; ENSG00000163162
GeneIDi284996
KEGGihsa:284996
UCSCiuc002taz.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRN149_HUMAN
AccessioniPrimary (citable) accession number: Q8NC42
Secondary accession number(s): Q53S14
, Q8N5I8, Q8NBY5, Q8WUU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 13, 2007
Last modified: May 23, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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