ID   ACBD4_HUMAN             Reviewed;         268 AA.
AC   Q8NC06; D3DX64; Q8IUT1; Q9H8Q4;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 2.
DT   24-JAN-2024, entry version 153.
DE   RecName: Full=Acyl-CoA-binding domain-containing protein 4;
GN   Name=ACBD4; ORFNames=HMFT0700;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC   TISSUE=Hepatoblastoma;
RX   PubMed=15221005; DOI=10.1038/sj.onc.1207782;
RA   Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S.,
RA   Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S.,
RA   Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A.;
RT   "Expression profiling and differential screening between hepatoblastomas
RT   and the corresponding normal livers: identification of high expression of
RT   the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas.";
RL   Oncogene 23:5901-5911(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Ovary, and Placenta;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC   TISSUE=Brain, and Colon;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166 AND SER-171, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 7-110 IN COMPLEX WITH THE
RP   ACYL-COA STEAROYL-COENZYME A.
RG   Structural genomics consortium (SGC);
RT   "Crystal structure of human acyl-CoA binding domain 4.";
RL   Submitted (APR-2009) to the PDB data bank.
CC   -!- FUNCTION: Binds medium- and long-chain acyl-CoA esters and may function
CC       as an intracellular carrier of acyl-CoA esters.
CC   -!- INTERACTION:
CC       Q8NC06-3; Q86V38: ATN1; NbExp=3; IntAct=EBI-12811089, EBI-11954292;
CC       Q8NC06-3; P02489: CRYAA; NbExp=3; IntAct=EBI-12811089, EBI-6875961;
CC       Q8NC06-3; G5E9A7: DMWD; NbExp=3; IntAct=EBI-12811089, EBI-10976677;
CC       Q8NC06-3; P22607: FGFR3; NbExp=3; IntAct=EBI-12811089, EBI-348399;
CC       Q8NC06-3; O14908-2: GIPC1; NbExp=3; IntAct=EBI-12811089, EBI-25913156;
CC       Q8NC06-3; P06396: GSN; NbExp=3; IntAct=EBI-12811089, EBI-351506;
CC       Q8NC06-3; Q92876: KLK6; NbExp=3; IntAct=EBI-12811089, EBI-2432309;
CC       Q8NC06-3; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-12811089, EBI-5235340;
CC       Q8NC06-3; Q8WW24: TEKT4; NbExp=3; IntAct=EBI-12811089, EBI-750487;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q8NC06-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8NC06-2; Sequence=VSP_014089;
CC       Name=3;
CC         IsoId=Q8NC06-3; Sequence=VSP_014088, VSP_014090;
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DR   EMBL; AB073387; BAD38638.1; -; mRNA.
DR   EMBL; AK023384; BAB14553.1; -; mRNA.
DR   EMBL; AK075104; BAC11403.1; -; mRNA.
DR   EMBL; CH471178; EAW51550.1; -; Genomic_DNA.
DR   EMBL; CH471178; EAW51551.1; -; Genomic_DNA.
DR   EMBL; CH471178; EAW51552.1; -; Genomic_DNA.
DR   EMBL; BC029164; AAH29164.1; -; mRNA.
DR   EMBL; BC041143; AAH41143.1; -; mRNA.
DR   CCDS; CCDS42348.1; -. [Q8NC06-2]
DR   CCDS; CCDS45710.1; -. [Q8NC06-3]
DR   CCDS; CCDS45711.1; -. [Q8NC06-1]
DR   RefSeq; NP_001129177.1; NM_001135705.2. [Q8NC06-2]
DR   RefSeq; NP_001129178.1; NM_001135706.2. [Q8NC06-3]
DR   RefSeq; NP_001129179.1; NM_001135707.2. [Q8NC06-1]
DR   RefSeq; NP_001308281.1; NM_001321352.1. [Q8NC06-3]
DR   RefSeq; NP_001308282.1; NM_001321353.1. [Q8NC06-3]
DR   RefSeq; NP_078998.1; NM_024722.3. [Q8NC06-2]
DR   RefSeq; XP_016880577.1; XM_017025088.1. [Q8NC06-3]
DR   RefSeq; XP_016880580.1; XM_017025091.1.
DR   RefSeq; XP_016880581.1; XM_017025092.1. [Q8NC06-2]
DR   PDB; 2WH5; X-ray; 2.60 A; A/B/C/D/E/F=7-110.
DR   PDBsum; 2WH5; -.
DR   AlphaFoldDB; Q8NC06; -.
DR   SMR; Q8NC06; -.
DR   BioGRID; 122878; 14.
DR   IntAct; Q8NC06; 11.
DR   STRING; 9606.ENSP00000405969; -.
DR   iPTMnet; Q8NC06; -.
DR   PhosphoSitePlus; Q8NC06; -.
DR   BioMuta; ACBD4; -.
DR   DMDM; 67460567; -.
DR   EPD; Q8NC06; -.
DR   jPOST; Q8NC06; -.
DR   MassIVE; Q8NC06; -.
DR   MaxQB; Q8NC06; -.
DR   PeptideAtlas; Q8NC06; -.
DR   ProteomicsDB; 72841; -. [Q8NC06-1]
DR   ProteomicsDB; 72842; -. [Q8NC06-2]
DR   ProteomicsDB; 72843; -. [Q8NC06-3]
DR   Antibodypedia; 29971; 160 antibodies from 28 providers.
DR   DNASU; 79777; -.
DR   Ensembl; ENST00000321854.13; ENSP00000314440.8; ENSG00000181513.15. [Q8NC06-2]
DR   Ensembl; ENST00000376955.8; ENSP00000366154.3; ENSG00000181513.15. [Q8NC06-1]
DR   Ensembl; ENST00000398322.7; ENSP00000381367.2; ENSG00000181513.15. [Q8NC06-2]
DR   Ensembl; ENST00000431281.5; ENSP00000405969.1; ENSG00000181513.15. [Q8NC06-3]
DR   Ensembl; ENST00000586346.5; ENSP00000465484.1; ENSG00000181513.15. [Q8NC06-3]
DR   Ensembl; ENST00000591859.5; ENSP00000465610.1; ENSG00000181513.15. [Q8NC06-3]
DR   Ensembl; ENST00000619916.4; ENSP00000482750.1; ENSG00000181513.15. [Q8NC06-1]
DR   GeneID; 79777; -.
DR   KEGG; hsa:79777; -.
DR   MANE-Select; ENST00000321854.13; ENSP00000314440.8; NM_001135705.3; NP_001129177.1. [Q8NC06-2]
DR   UCSC; uc002iic.4; human. [Q8NC06-1]
DR   AGR; HGNC:23337; -.
DR   CTD; 79777; -.
DR   DisGeNET; 79777; -.
DR   GeneCards; ACBD4; -.
DR   HGNC; HGNC:23337; ACBD4.
DR   HPA; ENSG00000181513; Tissue enhanced (liver).
DR   MIM; 619968; gene.
DR   neXtProt; NX_Q8NC06; -.
DR   OpenTargets; ENSG00000181513; -.
DR   PharmGKB; PA134897893; -.
DR   VEuPathDB; HostDB:ENSG00000181513; -.
DR   GeneTree; ENSGT00940000160739; -.
DR   HOGENOM; CLU_034436_1_0_1; -.
DR   InParanoid; Q8NC06; -.
DR   OMA; PLYRVIP; -.
DR   OrthoDB; 5402066at2759; -.
DR   PhylomeDB; Q8NC06; -.
DR   TreeFam; TF319446; -.
DR   PathwayCommons; Q8NC06; -.
DR   Reactome; R-HSA-390918; Peroxisomal lipid metabolism.
DR   SignaLink; Q8NC06; -.
DR   BioGRID-ORCS; 79777; 26 hits in 1160 CRISPR screens.
DR   ChiTaRS; ACBD4; human.
DR   EvolutionaryTrace; Q8NC06; -.
DR   GenomeRNAi; 79777; -.
DR   Pharos; Q8NC06; Tdark.
DR   PRO; PR:Q8NC06; -.
DR   Proteomes; UP000005640; Chromosome 17.
DR   RNAct; Q8NC06; Protein.
DR   Bgee; ENSG00000181513; Expressed in right lobe of liver and 128 other cell types or tissues.
DR   ExpressionAtlas; Q8NC06; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0000062; F:fatty-acyl-CoA binding; IBA:GO_Central.
DR   GO; GO:0006631; P:fatty acid metabolic process; IBA:GO_Central.
DR   CDD; cd00435; ACBP; 1.
DR   Gene3D; 1.20.80.10; -; 1.
DR   InterPro; IPR022408; Acyl-CoA-binding_prot_CS.
DR   InterPro; IPR000582; Acyl-CoA-binding_protein.
DR   InterPro; IPR035984; Acyl-CoA-binding_sf.
DR   InterPro; IPR014352; FERM/acyl-CoA-bd_prot_sf.
DR   PANTHER; PTHR23310:SF53; ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 4; 1.
DR   PANTHER; PTHR23310; ACYL-COA-BINDING PROTEIN, ACBP; 1.
DR   Pfam; PF00887; ACBP; 1.
DR   PRINTS; PR00689; ACOABINDINGP.
DR   SUPFAM; SSF47027; Acyl-CoA binding protein; 1.
DR   PROSITE; PS00880; ACB_1; 1.
DR   PROSITE; PS51228; ACB_2; 1.
DR   Genevisible; Q8NC06; HS.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; Lipid-binding; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..268
FT                   /note="Acyl-CoA-binding domain-containing protein 4"
FT                   /id="PRO_0000214032"
FT   DOMAIN          12..101
FT                   /note="ACB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00573"
FT   REGION          151..175
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         23..32
FT                   /ligand="an acyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:58342"
FT   BINDING         43..47
FT                   /ligand="an acyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:58342"
FT   BINDING         69
FT                   /ligand="an acyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:58342"
FT   BINDING         88
FT                   /ligand="an acyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:58342"
FT   MOD_RES         166
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:24275569"
FT   MOD_RES         171
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:24275569"
FT   VAR_SEQ         168..191
FT                   /note="ESHSPRDLDSEVFCDSLEQLEPEL -> ASLWAVTLPTPPQSPIHPGTWTPR
FT                   FSVIPWSSWSLSWFGQ (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15221005,
FT                   ECO:0000303|PubMed:15489334"
FT                   /id="VSP_014088"
FT   VAR_SEQ         192..268
FT                   /note="SSGQHLEESVIPGTAPCPPQRKRGCGAARRGPRSWTCGCWGQFEHYRRACRR
FT                   CRRGCRAWRACPGPLSSLTLSVRLE -> VWTEQRAASGGKRDPRNSPVPPTKKEGLRG
FT                   SPPGPQELDVWLLGTVRALQESMQEVQARVQSLESMPRPPEQRPQPRPSARPWPLGLPG
FT                   PALLFFLLWPFVVQWLFRMFRTQKR (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039,
FT                   ECO:0000303|PubMed:15489334"
FT                   /id="VSP_014089"
FT   VAR_SEQ         260..268
FT                   /note="SLTLSVRLE -> RGRSPGPVLGHGPLGSRGPRCSSSSCGPSSSSGSSECFG
FT                   PKRGDCQWRGLCSQLRLSCCALSLPRV (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15221005,
FT                   ECO:0000303|PubMed:15489334"
FT                   /id="VSP_014090"
FT   VARIANT         118
FT                   /note="P -> L (in dbSNP:rs901754)"
FT                   /id="VAR_055478"
FT   VARIANT         242
FT                   /note="R -> G (in dbSNP:rs16939879)"
FT                   /id="VAR_059109"
FT   CONFLICT        166
FT                   /note="S -> G (in Ref. 2; BAC11403)"
FT                   /evidence="ECO:0000305"
FT   HELIX           14..25
FT                   /evidence="ECO:0007829|PDB:2WH5"
FT   HELIX           36..51
FT                   /evidence="ECO:0007829|PDB:2WH5"
FT   HELIX           64..74
FT                   /evidence="ECO:0007829|PDB:2WH5"
FT   TURN            75..78
FT                   /evidence="ECO:0007829|PDB:2WH5"
FT   HELIX           81..100
FT                   /evidence="ECO:0007829|PDB:2WH5"
SQ   SEQUENCE   268 AA;  30308 MW;  0F337D89924CDE2E CRC64;
     MGTEKESPEP DCQKQFQAAV SVIQNLPKNG SYRPSYEEML RFYSYYKQAT MGPCLVPRPG
     FWDPIGRYKW DAWNSLGKMS REEAMSAYIT EMKLVAQKVI DTVPLGEVAE DMFGYFEPLY
     QVIPDMPRPP ETFLRRVTGW KEQVVNGDVG AVSEPPCLPK EPAPPSPESH SPRDLDSEVF
     CDSLEQLEPE LSSGQHLEES VIPGTAPCPP QRKRGCGAAR RGPRSWTCGC WGQFEHYRRA
     CRRCRRGCRA WRACPGPLSS LTLSVRLE
//