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Protein

Kinetochore protein Spc24

Gene

SPC24

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14738735). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:14738735). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein Spc24
Short name:
hSpc24
Gene namesi
Name:SPC24
Synonyms:SPBC24
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:26913. SPC24.

Subcellular locationi

GO - Cellular componenti

  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • Ndc80 complex Source: UniProtKB
  • nucleolus Source: HPA
  • nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162404412.

Polymorphism and mutation databases

BioMutaiSPC24.
DMDMi391358156.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 197197Kinetochore protein Spc24PRO_0000249559Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8NBT2.
MaxQBiQ8NBT2.
PaxDbiQ8NBT2.
PeptideAtlasiQ8NBT2.
PRIDEiQ8NBT2.
TopDownProteomicsiQ8NBT2-1. [Q8NBT2-1]

PTM databases

iPTMnetiQ8NBT2.
PhosphoSiteiQ8NBT2.

Expressioni

Gene expression databases

BgeeiENSG00000161888.
CleanExiHS_SPC24.
ExpressionAtlasiQ8NBT2. baseline and differential.
GenevisibleiQ8NBT2. HS.

Organism-specific databases

HPAiHPA051234.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SPC25Q9HBM16EBI-999900,EBI-999909

Protein-protein interaction databases

BioGridi127093. 46 interactions.
DIPiDIP-35754N.
IntActiQ8NBT2. 29 interactions.
MINTiMINT-4990252.
STRINGi9606.ENSP00000465075.

Structurei

Secondary structure

1
197
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi127 – 1315Combined sources
Turni134 – 1363Combined sources
Helixi137 – 14711Combined sources
Turni156 – 1583Combined sources
Beta strandi160 – 1634Combined sources
Beta strandi172 – 1754Combined sources
Helixi182 – 1909Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VE7X-ray2.88C/D122-197[»]
3IZ0electron microscopy8.60D/F122-197[»]
ProteinModelPortaliQ8NBT2.
SMRiQ8NBT2. Positions 112-197.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8NBT2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 131131Interaction with the N-terminus of SPBC25Add
BLAST
Regioni1 – 6969Interaction with the NDC80-CDCA1 subcomplexAdd
BLAST
Regioni132 – 19766Interaction with the C-terminus of SPBC25Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili33 – 13199Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the SPC24 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IYQI. Eukaryota.
ENOG4111TPD. LUCA.
HOGENOMiHOG000004855.
HOVERGENiHBG083027.
InParanoidiQ8NBT2.
KOiK11549.

Family and domain databases

InterProiIPR013252. Ndc80_Spc24.
[Graphical view]
PfamiPF08286. Spc24. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NBT2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAFRDIEEV SQGLLSLLGA NRAEAQQRRL LGRHEQVVER LLETQDGAEK
60 70 80 90 100
QLREILTMEK EVAQSLLNAK EQVHQGGVEL QQLEAGLQEA GEEDTRLKAS
110 120 130 140 150
LLYLTRELEE LKEIEADLER QEKEVDEDTT VTIPSAVYVA QLYHQVSKIE
160 170 180 190
WDYECEPGMV KGIHHGPSVA QPIHLDSTQL SRKFISDYLW SLVDTEW
Length:197
Mass (Da):22,478
Last modified:June 13, 2012 - v2
Checksum:iC5D99CE1F1A8F355
GO
Isoform 2 (identifier: Q8NBT2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     72-117: Missing.

Note: No experimental confirmation available.
Show »
Length:151
Mass (Da):17,316
Checksum:iCA84C4FEA046ECD2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti103 – 1031Y → Q in AAR88651 (PubMed:14738735).Curated
Sequence conflicti103 – 1031Y → Q in BAC11523 (PubMed:14702039).Curated
Sequence conflicti103 – 1031Y → Q in AAI05038 (PubMed:15489334).Curated
Sequence conflicti103 – 1031Y → Q in AAI05040 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei72 – 11746Missing in isoform 2. 1 PublicationVSP_054243Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY456387 mRNA. Translation: AAR88651.1.
AK075287 mRNA. Translation: BAC11523.1.
AK303157 mRNA. Translation: BAG64259.1.
AC011485 Genomic DNA. No translation available.
BC105037 mRNA. Translation: AAI05038.1.
BC105039 mRNA. Translation: AAI05040.1.
CCDSiCCDS45974.1. [Q8NBT2-1]
RefSeqiNP_001303960.1. NM_001317031.1.
NP_001303962.1. NM_001317033.1.
NP_872319.1. NM_182513.3.
XP_005259810.1. XM_005259753.3. [Q8NBT2-2]
UniGeneiHs.381225.
Hs.736069.

Genome annotation databases

EnsembliENST00000592540; ENSP00000465075; ENSG00000161888.
GeneIDi147841.
KEGGihsa:147841.
UCSCiuc002mql.3. human. [Q8NBT2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY456387 mRNA. Translation: AAR88651.1.
AK075287 mRNA. Translation: BAC11523.1.
AK303157 mRNA. Translation: BAG64259.1.
AC011485 Genomic DNA. No translation available.
BC105037 mRNA. Translation: AAI05038.1.
BC105039 mRNA. Translation: AAI05040.1.
CCDSiCCDS45974.1. [Q8NBT2-1]
RefSeqiNP_001303960.1. NM_001317031.1.
NP_001303962.1. NM_001317033.1.
NP_872319.1. NM_182513.3.
XP_005259810.1. XM_005259753.3. [Q8NBT2-2]
UniGeneiHs.381225.
Hs.736069.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VE7X-ray2.88C/D122-197[»]
3IZ0electron microscopy8.60D/F122-197[»]
ProteinModelPortaliQ8NBT2.
SMRiQ8NBT2. Positions 112-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127093. 46 interactions.
DIPiDIP-35754N.
IntActiQ8NBT2. 29 interactions.
MINTiMINT-4990252.
STRINGi9606.ENSP00000465075.

PTM databases

iPTMnetiQ8NBT2.
PhosphoSiteiQ8NBT2.

Polymorphism and mutation databases

BioMutaiSPC24.
DMDMi391358156.

Proteomic databases

EPDiQ8NBT2.
MaxQBiQ8NBT2.
PaxDbiQ8NBT2.
PeptideAtlasiQ8NBT2.
PRIDEiQ8NBT2.
TopDownProteomicsiQ8NBT2-1. [Q8NBT2-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000592540; ENSP00000465075; ENSG00000161888.
GeneIDi147841.
KEGGihsa:147841.
UCSCiuc002mql.3. human. [Q8NBT2-1]

Organism-specific databases

CTDi147841.
GeneCardsiSPC24.
HGNCiHGNC:26913. SPC24.
HPAiHPA051234.
MIMi609394. gene.
neXtProtiNX_Q8NBT2.
PharmGKBiPA162404412.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IYQI. Eukaryota.
ENOG4111TPD. LUCA.
HOGENOMiHOG000004855.
HOVERGENiHBG083027.
InParanoidiQ8NBT2.
KOiK11549.

Enzyme and pathway databases

ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-68877. Mitotic Prometaphase.

Miscellaneous databases

EvolutionaryTraceiQ8NBT2.
GeneWikiiSPC24.
GenomeRNAii147841.
PROiQ8NBT2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000161888.
CleanExiHS_SPC24.
ExpressionAtlasiQ8NBT2. baseline and differential.
GenevisibleiQ8NBT2. HS.

Family and domain databases

InterProiIPR013252. Ndc80_Spc24.
[Graphical view]
PfamiPF08286. Spc24. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPC24_HUMAN
AccessioniPrimary (citable) accession number: Q8NBT2
Secondary accession number(s): B4DZZ7, C9JGC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: June 13, 2012
Last modified: September 7, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.