Q8NBS9 (TXND5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thioredoxin domain-containing protein 5

Alternative name(s):
Endoplasmic reticulum resident protein 46
Short name=ER protein 46
Short name=ERp46
Thioredoxin-like protein p46

Gene names

Name:	TXNDC5
Synonyms:	TLP46
ORF Names:	UNQ364/PRO700

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 3 thioredoxin domains.
Sequence caution	The sequence AAH01199.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Coding sequence diversity	Alternative splicing
Domain	Redox-active center Repeat Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro cellular membrane organization Traceable author statement. Source: Reactome glycerol ether metabolic process Inferred from electronic annotation. Source: InterPro negative regulation of apoptotic process Traceable author statement PubMed 16130169. Source: UniProtKB post-Golgi vesicle-mediated transport Traceable author statement. Source: Reactome
Cellular_component	endoplasmic reticulum Traceable author statement PubMed 16130169. Source: UniProtKB endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell lysosomal lumen Traceable author statement. Source: Reactome
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro isomerase activity Inferred from electronic annotation. Source: InterPro protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NBS9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NBS9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 32

32

Potential

Chain

33 – 432

400

Thioredoxin domain-containing protein 5

PRO_0000034183

Regions

Domain

36 – 169

134

Thioredoxin 1

Domain

170 – 295

126

Thioredoxin 2

Domain

304 – 429

126

Thioredoxin 3

Motif

429 – 432

4

Prevents secretion from ER Potential

Amino acid modifications

Disulfide bond

89 ↔ 92

Redox-active Potential

Disulfide bond

217 ↔ 220

Redox-active Potential

Disulfide bond

350 ↔ 353

Redox-active Potential

Natural variations

Alternative sequence

1 – 108

108

Missing in isoform 2.

VSP_045181

Experimental info

Sequence conflict

354

1

K → R in BAC11526. Ref.2

Secondary structure

1

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432

Helix Strand Turn

Details...

Beta strand

324 – 326

3

Turn

329 – 331

3

Helix

332 – 336

5

Beta strand

337 – 346

10

Helix

351 – 364

14

Beta strand

374 – 380

7

Turn

381 – 383

3

Helix

385 – 390

6

Beta strand

395 – 403

9

Beta strand

406 – 411

6

Helix

417 – 427

11

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 11, 2003. Version 2. Checksum: 85E398008D6DB98C	FASTA	432	47,629
	10 20 30 40 50 60 MPARPGRLLP LLARPAALTA LLLLLLGHGG GGRWGARAQE AAAAAADGPP AADGEDGQDP 70 80 90 100 110 120 HSKHLYTADM FTHGIQSAAH FVMFFAPWCG HCQRLQPTWN DLGDKYNSME DAKVYVAKVD 130 140 150 160 170 180 CTAHSDVCSA QGVRGYPTLK LFKPGQEAVK YQGPRDFQTL ENWMLQTLNE EPVTPEPEVE 190 200 210 220 230 240 PPSAPELKQG LYELSASNFE LHVAQGDHFI KFFAPWCGHC KALAPTWEQL ALGLEHSETV 250 260 270 280 290 300 KIGKVDCTQH YELCSGNQVR GYPTLLWFRD GKKVDQYKGK RDLESLREYV ESQLQRTETG 310 320 330 340 350 360 ATETVTPSEA PVLAAEPEAD KGTVLALTEN NFDDTIAEGI TFIKFYAPWC GHCKTLAPTW 370 380 390 400 410 420 EELSKKEFPG LAGVKIAEVD CTAERNICSK YSVRGYPTLL LFRGGKKVSE HSGGRDLDSL 430 HRFVLSQAKD EL
	Isoform 2 [UniParc]. Checksum: 93FE17E484F5EFC9	FASTA	324	36,178
	10 20 30 40 50 60 MEDAKVYVAK VDCTAHSDVC SAQGVRGYPT LKLFKPGQEA VKYQGPRDFQ TLENWMLQTL 70 80 90 100 110 120 NEEPVTPEPE VEPPSAPELK QGLYELSASN FELHVAQGDH FIKFFAPWCG HCKALAPTWE 130 140 150 160 170 180 QLALGLEHSE TVKIGKVDCT QHYELCSGNQ VRGYPTLLWF RDGKKVDQYK GKRDLESLRE 190 200 210 220 230 240 YVESQLQRTE TGATETVTPS EAPVLAAEPE ADKGTVLALT ENNFDDTIAE GITFIKFYAP 250 260 270 280 290 300 WCGHCKTLAP TWEELSKKEF PGLAGVKIAE VDCTAERNIC SKYSVRGYPT LLLFRGGKKV 310 320 SEHSGGRDLD SLHRFVLSQA KDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Lung.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Lo S.L., Nissom P.M., Ong S.E., Choong M.L., Ng S.S., Lim J.W.E., Liang R.C.M.Y., Ou K., Seow T.K., Chung M.C.M. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 70-432 (ISOFORM 1). Tissue: Liver.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 82-432 (ISOFORM 1). Tissue: Eye.
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 189-432 (ISOFORMS 1/2). Tissue: Lymph node.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"The solution structure of the third thioredoxin domain of human thioredoxin domain-containing protein 5." RIKEN structural genomics initiative (RSGI) Submitted (SEP-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 322-432.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY358646 mRNA. Translation: AAQ89009.1.
AK075291 mRNA. Translation: BAC11526.1.
AK315598 mRNA. Translation: BAG37969.1.
AL023694 Genomic DNA. No translation available.
AL096800 Genomic DNA. No translation available.
AL133541 Genomic DNA. Translation: CAI19473.1.
CH471087 Genomic DNA. Translation: EAW55221.1.
AJ440721 mRNA. Translation: CAD29430.1.
BC001199 mRNA. Translation: AAH01199.1. Different initiation.
AL834423 mRNA. Translation: CAD39084.1.

IPI

IPI00171438.
IPI00939560.

RefSeq

NP_001139021.1. NM_001145549.2.
NP_110437.2. NM_030810.3.

UniGene

Hs.150837.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DIZ	NMR	-	A	322-432	[»]
3UJ1	X-ray	2.65	A	323-432	[»]
3UVT	X-ray	2.00	A/B/C/D/E	323-428	[»]

ProteinModelPortal

Q8NBS9.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8NBS9. 4 interactions.

MINT

MINT-5006305.

STRING

9606.ENSP00000369081.

PTM databases

PhosphoSite

Q8NBS9.

Polymorphism databases

DMDM

29839560.

Proteomic databases

PaxDb

Q8NBS9.

PRIDE

Q8NBS9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000379757; ENSP00000369081; ENSG00000239264.
ENST00000473453; ENSP00000420784; ENSG00000239264.

GeneID

81567.

KEGG

hsa:81567.

UCSC

uc003mxv.3. human.
uc010joa.2. human.

Organism-specific databases

CTD

81567.

GeneCards

GC06M007826.

HGNC

HGNC:21073. TXNDC5.

HPA

HPA034677.
HPA034678.

neXtProt

NX_Q8NBS9.

PharmGKB

PA134992492.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0526.

HOGENOM

HOG000007899.

HOVERGEN

HBG058611.

InParanoid

Q8NBS9.

KO

K13984.

OMA

SNFELHV.

OrthoDB

EOG40K804.

PhylomeDB

Q8NBS9.

Enzyme and pathway databases

Reactome

REACT_11123. Membrane Trafficking.

Gene expression databases

ArrayExpress

Q8NBS9.

Bgee

Q8NBS9.

CleanEx

HS_TXNDC5.

Genevestigator

Q8NBS9.

GermOnline

ENSG00000188428. Homo sapiens.

Family and domain databases

Gene3D

3.40.30.10. 3 hits.

InterPro

IPR005788. Disulphide_isomerase.
IPR005746. Thioredoxin.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]

Pfam

PF00085. Thioredoxin. 3 hits.
[Graphical view]

PRINTS

PR00421. THIOREDOXIN.

SUPFAM

SSF52833. Thiordxn-like_fd. 3 hits.

TIGRFAMs

TIGR01126. pdi_dom. 1 hit.

PROSITE

PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 3 hits.
PS51352. THIOREDOXIN_2. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

TXNDC5. human.

EvolutionaryTrace

Q8NBS9.

GenomeRNAi

81567.

NextBio

71856.

Entry information

Entry name

TXND5_HUMAN

Accession

Primary (citable) accession number: Q8NBS9
Secondary accession number(s): B2RDM2 , Q5TCQ0, Q8ND33, Q8TCT2, Q9BVH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	April 11, 2003
Last modified:	May 29, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.