Reviewed, UniProtKB/Swiss-Prot Q8NBQ5 (DHB11_HUMAN)

Names and origin

Protein names

Recommended name:
    Estradiol 17-beta-dehydrogenase 11
    EC=1.1.1.62
Alternative name(s):
    17-beta-hydroxysteroid dehydrogenase 11
      Short name=17-beta-HSD 11
      Short name=17betaHSD11
      Short name=17bHSD11
    17-beta-HSD XI
      Short name=17betaHSDXI
    Dehydrogenase/reductase SDR family member 8
    Retinal short-chain dehydrogenase/reductase 2
      Short name=retSDR2
    Cutaneous T-cell lymphoma-associated antigen HD-CL-03
    CTCL tumor antigen HD-CL-03

Gene names

Name:	HSD17B11
Synonyms:	DHRS8, PAN1B
ORF Names:	PSEC0029, UNQ207/PRO233

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can convert androstan-3-alpha,17-beta-diol (3-alpha-diol) to androsterone in vitro, suggesting that it may participate in androgen metabolism during steroidogenesis. May act by metabolizing compounds that stimulate steroid synthesis and/or by generating metabolites that inhibit it. Has no activity toward DHEA (dehydroepiandrosterone), or A-dione (4-androste-3,17-dione), and only a slight activity toward testosterone to A-dione. Tumor-associated antigen in cutaneous T-cell lymphoma.
Catalytic activity	Estradiol-17-beta + NAD(P)+ = estrone + NAD(P)H. Ref.8
Subcellular location	Secreted Potential.
Tissue specificity	Present at high level in steroidogenic cells such as syncytiotrophoblasts, sebaceous gland, Leydig cells, and granulosa cells of the dominant follicle and corpus luteum. In lung, it is detected in the ciliated epithelium and in acini of adult trachea, in bronchioles, but not in alveoli. In the eye, it is detected in the nonpigmented epithelium of the ciliary body and, at lower level, in the inner nuclear layer of the retina (at protein level). Widely expressed. Highly expressed in retina, pancreas, kidney, liver, lung, adrenal, small intestine, ovary and heart. Ref.8 Ref.1 Ref.6 Ref.7
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Cellular component	Secreted
Domain	Signal
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	androgen catabolic process Ref.8 Inferred from direct assay. Source: HGNC oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Ref.8 Inferred from direct assay. Source: HGNC extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Chain

20 – 300

281

Estradiol 17-beta-dehydrogenase 11

PRO_0000031970

Regions

Nucleotide binding

40 – 64

25

NADP By similarity

Sites

Active site

185

1

Proton acceptor By similarity

Binding site

172

1

Substrate By similarity

Experimental info

Sequence conflict

23

1

V → E in AAH08650. Ref.5

Sequence conflict

38

1

I → T in BAC11560. Ref.4

Sequence conflict

106

1

K → N in AAH08650. Ref.5

Sequence conflict

107

1

V → C in AAH08650. Ref.5

Sequence conflict

228

1

N → K in AAH08650. Ref.5

Sequence conflict

229

1

P → M in AAH08650. Ref.5

Sequence conflict

263

1

I → N in AAH08650. Ref.5

Sequence conflict

264

1

A → C in AAH08650. Ref.5

Sequence conflict

265

1

F → I in AAH08650. Ref.5

Secondary structure

1

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300

Helix Strand Turn

Details...

Beta strand

38 – 42

5

Turn

43 – 45

3

Helix

47 – 58

12

Beta strand

62 – 68

7

Helix

70 – 82

13

Beta strand

87 – 91

5

Helix

97 – 110

14

Beta strand

115 – 119

5

Helix

130 – 132

3

Helix

133 – 144

12

Helix

146 – 161

16

Beta strand

165 – 170

6

Helix

179 – 205

27

Beta strand

211 – 218

8

Helix

220 – 223

4

Helix

230 – 233

4

Helix

239 – 251

13

Beta strand

255 – 259

5

Helix

265 – 270

6

Sequences

	Sequence		Length	Mass (Da)
	Q8NBQ5-1 [UniParc]. Last modified August 16, 2005. Version 2. Checksum: 51DC0EAA205EBE86	FASTA	300	32,964
	10 20 30 40 50 60 MKFLLDILLL LPLLIVCSLE SFVKLFIPKR RKSVTGEIVL ITGAGHGIGR LTAYEFAKLK 70 80 90 100 110 120 SKLVLWDINK HGLEETAAKC KGLGAKVHTF VVDCSNREDI YSSAKKVKAE IGDVSILVNN 130 140 150 160 170 180 AGVVYTSDLF ATQDPQIEKT FEVNVLAHFW TTKAFLPAMT KNNHGHIVTV ASAAGHVSVP 190 200 210 220 230 240 FLLAYCSSKF AAVGFHKTLT DELAALQITG VKTTCLCPNF VNTGFIKNPS TSLGPTLEPE 250 260 270 280 290 300 EVVNRLMHGI LTEQKMIFIP SSIAFLTTLE RILPERFLAV LKRKISVKFD AVIGYKMKAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Short-chain dehydrogenases/reductases in retina." Haeseleer F., Palczewski K. Methods Enzymol. 316:372-383(2000) [PubMed: 10800688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Retina.
[2]	"SEREX identification of new tumour-associated antigens in cutaneous T-cell lymphoma." Hartmann T.B., Thiel D., Dummer R., Schadendorf D., Eichmueller S. Br. J. Dermatol. 150:252-258(2004) [PubMed: 14996095] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lymphoma.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"HRI human cDNA sequencing project." Ota T., Nishikawa T., Suzuki Y., Kawai-Hio Y., Hayashi K., Ishii S., Saito K., Yamamoto J., Wakamatsu A., Nagai T., Nakamura Y., Nagahari K., Sugano S., Isogai T. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow, Colon, Kidney, Liver and Urinary bladder.
[6]	"Cloning and expression of a novel tissue specific 17beta-hydroxysteroid dehydrogenase." Li K.X.Z., Smith R.E., Krozowski Z.S. Endocr. Res. 24:663-667(1998) [PubMed: 9888557] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Pan1b (17betaHSD11)-enzymatic activity and distribution in the lung." Brereton P., Suzuki T., Sasano H., Li K., Duarte C., Obeyesekere V., Haeseleer F., Palczewski K., Smith I., Komesaroff P., Krozowski Z. Mol. Cell. Endocrinol. 171:111-117(2001) [PubMed: 11165019] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"17 beta-hydroxysteroid dehydrogenase type XI localizes to human steroidogenic cells." Chai Z., Brereton P., Suzuki T., Sasano H., Obeyesekere V., Escher G., Saffery R., Fuller P., Enriquez C., Krozowski Z. Endocrinology 144:2084-2091(2003) [PubMed: 12697717] [Abstract] Cited for: ENZYME ACTIVITY IN VITRO, TISSUE SPECIFICITY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[10]	"Crystal structure of human 17-beta-hydroxysteroid dehydrogenase type XI." Lukacik P., Bunkoczi G., Kavanagh K., Ng S., Von delft F., Bray J., Edwards A., Arrowsmith C., Sundstrom M., Oppermann U. Submitted (DEC-2004) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 28-275.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF126780 mRNA. Translation: AAF06939.1.
AF273056 mRNA. Translation: AAM44459.1.
AY358553 mRNA. Translation: AAQ88917.1.
AK075348 mRNA. Translation: BAC11560.1.
BC008650 mRNA. Translation: AAH08650.1.
BC014327 mRNA. Translation: AAH14327.1.
BC016367 mRNA. Translation: AAH16367.1.
BC021673 mRNA. Translation: AAH21673.1.
BC036001 mRNA. Translation: AAH36001.1.

IPI

IPI00329598.

RefSeq

NP_057329.2.

UniGene

Hs.709454

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YB1	X-ray	1.95	A/B	28-275	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8NBQ5. 3 interactions.

Proteomic databases

PRIDE

Q8NBQ5.

Genome annotation databases

Ensembl

ENSG00000198189. Homo sapiens. [Contig view]

GeneID

51170.

KEGG

hsa:51170.

Organism-specific databases

GeneCards

GC04M088477.

H-InvDB

HIX0004358.

HGNC

HGNC:22960. HSD17B11.

PharmGKB

PA134981822.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q8NBQ5.

HOVERGEN

Q8NBQ5.

Enzyme and pathway databases

BRENDA

1.1.1.62. 247.

Gene expression databases

ArrayExpress

Q8NBQ5.

Bgee

Q8NBQ5.

CleanEx

HS_HSD17B11.

GermOnline

ENSG00000198189. Homo sapiens.

Family and domain databases

InterPro

IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR19410. ADH_short_C2. 1 hit.

Pfam

PF00106. adh_short. 1 hit.
[Graphical view]

PRINTS

PR00081. GDHRDH.
PR00080. SDRFAMILY.

PROSITE

PS00061. ADH_SHORT. False negative.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

54111.

Entry information

Entry name

DHB11_HUMAN

Accession

Primary (citable) accession number: Q8NBQ5
Secondary accession number(s): Q96HF6, Q9UKU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	August 16, 2005
Last modified:	June 16, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)