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Q8NBJ5

- GT251_HUMAN

UniProt

Q8NBJ5 - GT251_HUMAN

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Protein

Procollagen galactosyltransferase 1

Gene
COLGALT1, GLT25D1, PSEC0241
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Has a beta-galactosyltransferase activity; transfers beta-galactose to hydroxylysine residues of collagen.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + 5-hydroxy-L-lysine-[procollagen] = UDP + 5-(D-galactosyloxy)-L-lysine-[procollagen].1 Publication

Kineticsi

  1. KM=18.7 µM for UDP-galactose1 Publication

GO - Molecular functioni

  1. procollagen galactosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. extracellular matrix organization Source: Reactome
  2. lipopolysaccharide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_121139. Collagen biosynthesis and modifying enzymes.
SABIO-RKQ8NBJ5.

Protein family/group databases

CAZyiGT25. Glycosyltransferase Family 25.

Names & Taxonomyi

Protein namesi
Recommended name:
Procollagen galactosyltransferase 1 (EC:2.4.1.50)
Alternative name(s):
Collagen beta(1-O)galactosyltransferase 1
Glycosyltransferase 25 family member 1
Hydroxylysine galactosyltransferase 1
Gene namesi
Synonyms:GLT25D1
ORF Names:PSEC0241
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:26182. COLGALT1.

Subcellular locationi

Endoplasmic reticulum lumen
Note: Colocalized with PLOD3 and mannose binding lectin.1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134991138.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed predictionAdd
BLAST
Chaini30 – 622593Procollagen galactosyltransferase 1PRO_0000309536Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi96 – 961N-linked (GlcNAc...)1 Publication
Glycosylationi184 – 1841N-linked (GlcNAc...) Reviewed prediction
Glycosylationi381 – 3811N-linked (GlcNAc...)1 Publication

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8NBJ5.
PaxDbiQ8NBJ5.
PeptideAtlasiQ8NBJ5.
PRIDEiQ8NBJ5.

PTM databases

PhosphoSiteiQ8NBJ5.

Expressioni

Tissue specificityi

Ubiquitous with higher levels in placenta, heart, lung and spleen.1 Publication

Gene expression databases

ArrayExpressiQ8NBJ5.
BgeeiQ8NBJ5.
CleanExiHS_GLT25D1.
GenevestigatoriQ8NBJ5.

Organism-specific databases

HPAiHPA047821.

Interactioni

Protein-protein interaction databases

BioGridi122826. 11 interactions.
IntActiQ8NBJ5. 4 interactions.
STRINGi9606.ENSP00000252599.

Structurei

3D structure databases

ProteinModelPortaliQ8NBJ5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi619 – 6224Prevents secretion from ER

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG293154.
HOGENOMiHOG000007198.
HOVERGENiHBG058097.
InParanoidiQ8NBJ5.
KOiK11703.
OMAiMWADYIL.
OrthoDBiEOG7060RC.
PhylomeDBiQ8NBJ5.
TreeFamiTF313826.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR002654. Glyco_trans_25.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01755. Glyco_transf_25. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8NBJ5-1 [UniParc]FASTAAdd to Basket

« Hide

MAAAPRAGRR RGQPLLALLL LLLAPLPPGA PPGADAYFPE ERWSPESPLQ    50
APRVLIALLA RNAAHALPTT LGALERLRHP RERTALWVAT DHNMDNTSTV 100
LREWLVAVKS LYHSVEWRPA EEPRSYPDEE GPKHWSDSRY EHVMKLRQAA 150
LKSARDMWAD YILFVDADNL ILNPDTLSLL IAENKTVVAP MLDSRAAYSN 200
FWCGMTSQGY YKRTPAYIPI RKRDRRGCFA VPMVHSTFLI DLRKAASRNL 250
AFYPPHPDYT WSFDDIIVFA FSCKQAEVQM YVCNKEEYGF LPVPLRAHST 300
LQDEAESFMH VQLEVMVKHP PAEPSRFISA PTKTPDKMGF DEVFMINLRR 350
RQDRRERMLR ALQAQEIECR LVEAVDGKAM NTSQVEALGI QMLPGYRDPY 400
HGRPLTKGEL GCFLSHYNIW KEVVDRGLQK SLVFEDDLRF EIFFKRRLMN 450
LMRDVEREGL DWDLIYVGRK RMQVEHPEKA VPRVRNLVEA DYSYWTLAYV 500
ISLQGARKLL AAEPLSKMLP VDEFLPVMFD KHPVSEYKAH FSLRNLHAFS 550
VEPLLIYPTH YTGDDGYVSD TETSVVWNNE HVKTDWDRAK SQKMREQQAL 600
SREAKNSDVL QSPLDSAARD EL 622
Length:622
Mass (Da):71,636
Last modified:October 1, 2002 - v1
Checksum:iC430974CB1CF5280
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti100 – 1001V → E in BAC11307. 1 Publication
Sequence conflicti220 – 2201I → T in BAC11307. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK074941 mRNA. Translation: BAC11307.1.
AK075541 mRNA. Translation: BAC11684.1.
AC010618 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84622.1.
BC108308 mRNA. Translation: AAI08309.1.
CCDSiCCDS12363.1.
RefSeqiNP_078932.2. NM_024656.2.
UniGeneiHs.418795.

Genome annotation databases

EnsembliENST00000252599; ENSP00000252599; ENSG00000130309.
GeneIDi79709.
KEGGihsa:79709.
UCSCiuc002nhc.1. human.

Polymorphism databases

DMDMi74715064.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK074941 mRNA. Translation: BAC11307.1 .
AK075541 mRNA. Translation: BAC11684.1 .
AC010618 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84622.1 .
BC108308 mRNA. Translation: AAI08309.1 .
CCDSi CCDS12363.1.
RefSeqi NP_078932.2. NM_024656.2.
UniGenei Hs.418795.

3D structure databases

ProteinModelPortali Q8NBJ5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 122826. 11 interactions.
IntActi Q8NBJ5. 4 interactions.
STRINGi 9606.ENSP00000252599.

Protein family/group databases

CAZyi GT25. Glycosyltransferase Family 25.

PTM databases

PhosphoSitei Q8NBJ5.

Polymorphism databases

DMDMi 74715064.

Proteomic databases

MaxQBi Q8NBJ5.
PaxDbi Q8NBJ5.
PeptideAtlasi Q8NBJ5.
PRIDEi Q8NBJ5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000252599 ; ENSP00000252599 ; ENSG00000130309 .
GeneIDi 79709.
KEGGi hsa:79709.
UCSCi uc002nhc.1. human.

Organism-specific databases

CTDi 79709.
GeneCardsi GC19P017747.
HGNCi HGNC:26182. COLGALT1.
HPAi HPA047821.
neXtProti NX_Q8NBJ5.
PharmGKBi PA134991138.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG293154.
HOGENOMi HOG000007198.
HOVERGENi HBG058097.
InParanoidi Q8NBJ5.
KOi K11703.
OMAi MWADYIL.
OrthoDBi EOG7060RC.
PhylomeDBi Q8NBJ5.
TreeFami TF313826.

Enzyme and pathway databases

Reactomei REACT_121139. Collagen biosynthesis and modifying enzymes.
SABIO-RK Q8NBJ5.

Miscellaneous databases

ChiTaRSi GLT25D1. human.
GenomeRNAii 79709.
NextBioi 69027.
PROi Q8NBJ5.

Gene expression databases

ArrayExpressi Q8NBJ5.
Bgeei Q8NBJ5.
CleanExi HS_GLT25D1.
Genevestigatori Q8NBJ5.

Family and domain databases

Gene3Di 3.90.550.10. 2 hits.
InterProi IPR002654. Glyco_trans_25.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
Pfami PF01755. Glyco_transf_25. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
    Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
    , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
    DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Teratocarcinoma.
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Cervix.
  5. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-96 AND ASN-381.
    Tissue: Liver.
  6. "Core glycosylation of collagen is initiated by two beta(1-O)galactosyltransferases."
    Schegg B., Huelsmeier A.J., Rutschmann C., Maag C., Hennet T.
    Mol. Cell. Biol. 29:943-952(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
  7. "The human collagen beta(1-O)galactosyltransferase, GLT25D1, is a soluble endoplasmic reticulum localized protein."
    Liefhebber J.M., Punt S., Spaan W.J., van Leeuwen H.C.
    BMC Cell Biol. 11:33-33(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, IDENTIFICATION BY MASS SPECTROMETRY.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGT251_HUMAN
AccessioniPrimary (citable) accession number: Q8NBJ5
Secondary accession number(s): Q8NC64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: October 1, 2002
Last modified: September 3, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Has no glucosyltransferase activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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