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Q8NBJ5 (GT251_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Procollagen galactosyltransferase 1

EC=2.4.1.50
Alternative name(s):
Collagen beta(1-O)galactosyltransferase 1
Glycosyltransferase 25 family member 1
Hydroxylysine galactosyltransferase 1
Gene names
Name:COLGALT1
Synonyms:GLT25D1
ORF Names:PSEC0241
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length622 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has a beta-galactosyltransferase activity; transfers beta-galactose to hydroxylysine residues of collagen. Ref.6

Catalytic activity

UDP-alpha-D-galactose + 5-hydroxy-L-lysine-[procollagen] = UDP + 5-(D-galactosyloxy)-L-lysine-[procollagen]. Ref.6

Subcellular location

Endoplasmic reticulum lumen. Note: Colocalized with PLOD3 and mannose binding lectin. Ref.7

Tissue specificity

Ubiquitous with higher levels in placenta, heart, lung and spleen. Ref.6

Post-translational modification

N-glycosylated. Ref.7

Sequence similarities

Belongs to the glycosyltransferase 25 family.

Caution

Has no glucosyltransferase activity.

Biophysicochemical properties

Kinetic parameters:

KM=18.7 µM for UDP-galactose Ref.6

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
   DomainSignal
   Molecular functionGlycosyltransferase
Transferase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processextracellular matrix organization

Traceable author statement. Source: Reactome

lipopolysaccharide biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentendoplasmic reticulum lumen

Inferred from direct assay Ref.7. Source: UniProtKB

   Molecular_functionprocollagen galactosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 622593Procollagen galactosyltransferase 1
PRO_0000309536

Regions

Motif619 – 6224Prevents secretion from ER

Amino acid modifications

Glycosylation961N-linked (GlcNAc...) Ref.5
Glycosylation1841N-linked (GlcNAc...) Potential
Glycosylation3811N-linked (GlcNAc...) Ref.5

Experimental info

Sequence conflict1001V → E in BAC11307. Ref.1
Sequence conflict2201I → T in BAC11307. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8NBJ5 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: C430974CB1CF5280

FASTA62271,636
        10         20         30         40         50         60 
MAAAPRAGRR RGQPLLALLL LLLAPLPPGA PPGADAYFPE ERWSPESPLQ APRVLIALLA 

        70         80         90        100        110        120 
RNAAHALPTT LGALERLRHP RERTALWVAT DHNMDNTSTV LREWLVAVKS LYHSVEWRPA 

       130        140        150        160        170        180 
EEPRSYPDEE GPKHWSDSRY EHVMKLRQAA LKSARDMWAD YILFVDADNL ILNPDTLSLL 

       190        200        210        220        230        240 
IAENKTVVAP MLDSRAAYSN FWCGMTSQGY YKRTPAYIPI RKRDRRGCFA VPMVHSTFLI 

       250        260        270        280        290        300 
DLRKAASRNL AFYPPHPDYT WSFDDIIVFA FSCKQAEVQM YVCNKEEYGF LPVPLRAHST 

       310        320        330        340        350        360 
LQDEAESFMH VQLEVMVKHP PAEPSRFISA PTKTPDKMGF DEVFMINLRR RQDRRERMLR 

       370        380        390        400        410        420 
ALQAQEIECR LVEAVDGKAM NTSQVEALGI QMLPGYRDPY HGRPLTKGEL GCFLSHYNIW 

       430        440        450        460        470        480 
KEVVDRGLQK SLVFEDDLRF EIFFKRRLMN LMRDVEREGL DWDLIYVGRK RMQVEHPEKA 

       490        500        510        520        530        540 
VPRVRNLVEA DYSYWTLAYV ISLQGARKLL AAEPLSKMLP VDEFLPVMFD KHPVSEYKAH 

       550        560        570        580        590        600 
FSLRNLHAFS VEPLLIYPTH YTGDDGYVSD TETSVVWNNE HVKTDWDRAK SQKMREQQAL 

       610        620 
SREAKNSDVL QSPLDSAARD EL 

« Hide

References

« Hide 'large scale' references
[1]"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. expand/collapse author list , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Teratocarcinoma.
[2]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Cervix.
[5]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-96 AND ASN-381.
Tissue: Liver.
[6]"Core glycosylation of collagen is initiated by two beta(1-O)galactosyltransferases."
Schegg B., Huelsmeier A.J., Rutschmann C., Maag C., Hennet T.
Mol. Cell. Biol. 29:943-952(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
[7]"The human collagen beta(1-O)galactosyltransferase, GLT25D1, is a soluble endoplasmic reticulum localized protein."
Liefhebber J.M., Punt S., Spaan W.J., van Leeuwen H.C.
BMC Cell Biol. 11:33-33(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, IDENTIFICATION BY MASS SPECTROMETRY.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK074941 mRNA. Translation: BAC11307.1.
AK075541 mRNA. Translation: BAC11684.1.
AC010618 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84622.1.
BC108308 mRNA. Translation: AAI08309.1.
RefSeqNP_078932.2. NM_024656.2.
UniGeneHs.418795.

3D structure databases

ProteinModelPortalQ8NBJ5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122826. 10 interactions.
IntActQ8NBJ5. 4 interactions.
STRING9606.ENSP00000252599.

Protein family/group databases

CAZyGT25. Glycosyltransferase Family 25.

PTM databases

PhosphoSiteQ8NBJ5.

Polymorphism databases

DMDM74715064.

Proteomic databases

PaxDbQ8NBJ5.
PeptideAtlasQ8NBJ5.
PRIDEQ8NBJ5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000252599; ENSP00000252599; ENSG00000130309.
GeneID79709.
KEGGhsa:79709.
UCSCuc002nhc.1. human.

Organism-specific databases

CTD79709.
GeneCardsGC19P017747.
HGNCHGNC:26182. COLGALT1.
HPAHPA047821.
neXtProtNX_Q8NBJ5.
PharmGKBPA134991138.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG293154.
HOGENOMHOG000007198.
HOVERGENHBG058097.
InParanoidQ8NBJ5.
KOK11703.
OMAMWADYIL.
OrthoDBEOG7060RC.
PhylomeDBQ8NBJ5.
TreeFamTF313826.

Enzyme and pathway databases

ReactomeREACT_118779. Extracellular matrix organization.
SABIO-RKQ8NBJ5.

Gene expression databases

ArrayExpressQ8NBJ5.
BgeeQ8NBJ5.
CleanExHS_GLT25D1.
GenevestigatorQ8NBJ5.

Family and domain databases

InterProIPR002654. Glyco_trans_25.
[Graphical view]
PfamPF01755. Glyco_transf_25. 1 hit.
[Graphical view]
PROSITEPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGLT25D1. human.
GenomeRNAi79709.
NextBio69027.
PROQ8NBJ5.

Entry information

Entry nameGT251_HUMAN
AccessionPrimary (citable) accession number: Q8NBJ5
Secondary accession number(s): Q8NC64
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM