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Q8NBJ5

- GT251_HUMAN

UniProt

Q8NBJ5 - GT251_HUMAN

Protein

Procollagen galactosyltransferase 1

Gene

COLGALT1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Has a beta-galactosyltransferase activity; transfers beta-galactose to hydroxylysine residues of collagen.1 Publication

    Catalytic activityi

    UDP-alpha-D-galactose + 5-hydroxy-L-lysine-[procollagen] = UDP + 5-(D-galactosyloxy)-L-lysine-[procollagen].1 Publication

    Kineticsi

    1. KM=18.7 µM for UDP-galactose1 Publication

    GO - Molecular functioni

    1. procollagen galactosyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. extracellular matrix organization Source: Reactome
    2. lipopolysaccharide biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_121139. Collagen biosynthesis and modifying enzymes.
    SABIO-RKQ8NBJ5.

    Protein family/group databases

    CAZyiGT25. Glycosyltransferase Family 25.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Procollagen galactosyltransferase 1 (EC:2.4.1.50)
    Alternative name(s):
    Collagen beta(1-O)galactosyltransferase 1
    Glycosyltransferase 25 family member 1
    Hydroxylysine galactosyltransferase 1
    Gene namesi
    Name:COLGALT1
    Synonyms:GLT25D1
    ORF Names:PSEC0241
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:26182. COLGALT1.

    Subcellular locationi

    Endoplasmic reticulum lumen 1 PublicationPROSITE-ProRule annotation
    Note: Colocalized with PLOD3 and mannose binding lectin.

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB
    2. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134991138.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 622593Procollagen galactosyltransferase 1PRO_0000309536Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi96 – 961N-linked (GlcNAc...)2 Publications
    Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi381 – 3811N-linked (GlcNAc...)2 Publications

    Post-translational modificationi

    N-glycosylated.2 Publications

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ8NBJ5.
    PaxDbiQ8NBJ5.
    PeptideAtlasiQ8NBJ5.
    PRIDEiQ8NBJ5.

    PTM databases

    PhosphoSiteiQ8NBJ5.

    Expressioni

    Tissue specificityi

    Ubiquitous with higher levels in placenta, heart, lung and spleen.1 Publication

    Gene expression databases

    ArrayExpressiQ8NBJ5.
    BgeeiQ8NBJ5.
    CleanExiHS_GLT25D1.
    GenevestigatoriQ8NBJ5.

    Organism-specific databases

    HPAiHPA047821.

    Interactioni

    Protein-protein interaction databases

    BioGridi122826. 11 interactions.
    IntActiQ8NBJ5. 4 interactions.
    STRINGi9606.ENSP00000252599.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NBJ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi619 – 6224Prevents secretion from ER

    Sequence similaritiesi

    Belongs to the glycosyltransferase 25 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG293154.
    HOGENOMiHOG000007198.
    HOVERGENiHBG058097.
    InParanoidiQ8NBJ5.
    KOiK11703.
    OMAiMWADYIL.
    OrthoDBiEOG7060RC.
    PhylomeDBiQ8NBJ5.
    TreeFamiTF313826.

    Family and domain databases

    Gene3Di3.90.550.10. 2 hits.
    InterProiIPR002654. Glyco_trans_25.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF01755. Glyco_transf_25. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8NBJ5-1 [UniParc]FASTAAdd to Basket

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    MAAAPRAGRR RGQPLLALLL LLLAPLPPGA PPGADAYFPE ERWSPESPLQ    50
    APRVLIALLA RNAAHALPTT LGALERLRHP RERTALWVAT DHNMDNTSTV 100
    LREWLVAVKS LYHSVEWRPA EEPRSYPDEE GPKHWSDSRY EHVMKLRQAA 150
    LKSARDMWAD YILFVDADNL ILNPDTLSLL IAENKTVVAP MLDSRAAYSN 200
    FWCGMTSQGY YKRTPAYIPI RKRDRRGCFA VPMVHSTFLI DLRKAASRNL 250
    AFYPPHPDYT WSFDDIIVFA FSCKQAEVQM YVCNKEEYGF LPVPLRAHST 300
    LQDEAESFMH VQLEVMVKHP PAEPSRFISA PTKTPDKMGF DEVFMINLRR 350
    RQDRRERMLR ALQAQEIECR LVEAVDGKAM NTSQVEALGI QMLPGYRDPY 400
    HGRPLTKGEL GCFLSHYNIW KEVVDRGLQK SLVFEDDLRF EIFFKRRLMN 450
    LMRDVEREGL DWDLIYVGRK RMQVEHPEKA VPRVRNLVEA DYSYWTLAYV 500
    ISLQGARKLL AAEPLSKMLP VDEFLPVMFD KHPVSEYKAH FSLRNLHAFS 550
    VEPLLIYPTH YTGDDGYVSD TETSVVWNNE HVKTDWDRAK SQKMREQQAL 600
    SREAKNSDVL QSPLDSAARD EL 622
    Length:622
    Mass (Da):71,636
    Last modified:October 1, 2002 - v1
    Checksum:iC430974CB1CF5280
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti100 – 1001V → E in BAC11307. (PubMed:16303743)Curated
    Sequence conflicti220 – 2201I → T in BAC11307. (PubMed:16303743)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK074941 mRNA. Translation: BAC11307.1.
    AK075541 mRNA. Translation: BAC11684.1.
    AC010618 Genomic DNA. No translation available.
    CH471106 Genomic DNA. Translation: EAW84622.1.
    BC108308 mRNA. Translation: AAI08309.1.
    CCDSiCCDS12363.1.
    RefSeqiNP_078932.2. NM_024656.2.
    UniGeneiHs.418795.

    Genome annotation databases

    EnsembliENST00000252599; ENSP00000252599; ENSG00000130309.
    GeneIDi79709.
    KEGGihsa:79709.
    UCSCiuc002nhc.1. human.

    Polymorphism databases

    DMDMi74715064.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK074941 mRNA. Translation: BAC11307.1 .
    AK075541 mRNA. Translation: BAC11684.1 .
    AC010618 Genomic DNA. No translation available.
    CH471106 Genomic DNA. Translation: EAW84622.1 .
    BC108308 mRNA. Translation: AAI08309.1 .
    CCDSi CCDS12363.1.
    RefSeqi NP_078932.2. NM_024656.2.
    UniGenei Hs.418795.

    3D structure databases

    ProteinModelPortali Q8NBJ5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122826. 11 interactions.
    IntActi Q8NBJ5. 4 interactions.
    STRINGi 9606.ENSP00000252599.

    Protein family/group databases

    CAZyi GT25. Glycosyltransferase Family 25.

    PTM databases

    PhosphoSitei Q8NBJ5.

    Polymorphism databases

    DMDMi 74715064.

    Proteomic databases

    MaxQBi Q8NBJ5.
    PaxDbi Q8NBJ5.
    PeptideAtlasi Q8NBJ5.
    PRIDEi Q8NBJ5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000252599 ; ENSP00000252599 ; ENSG00000130309 .
    GeneIDi 79709.
    KEGGi hsa:79709.
    UCSCi uc002nhc.1. human.

    Organism-specific databases

    CTDi 79709.
    GeneCardsi GC19P017747.
    HGNCi HGNC:26182. COLGALT1.
    HPAi HPA047821.
    neXtProti NX_Q8NBJ5.
    PharmGKBi PA134991138.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG293154.
    HOGENOMi HOG000007198.
    HOVERGENi HBG058097.
    InParanoidi Q8NBJ5.
    KOi K11703.
    OMAi MWADYIL.
    OrthoDBi EOG7060RC.
    PhylomeDBi Q8NBJ5.
    TreeFami TF313826.

    Enzyme and pathway databases

    Reactomei REACT_121139. Collagen biosynthesis and modifying enzymes.
    SABIO-RK Q8NBJ5.

    Miscellaneous databases

    ChiTaRSi GLT25D1. human.
    GenomeRNAii 79709.
    NextBioi 69027.
    PROi Q8NBJ5.

    Gene expression databases

    ArrayExpressi Q8NBJ5.
    Bgeei Q8NBJ5.
    CleanExi HS_GLT25D1.
    Genevestigatori Q8NBJ5.

    Family and domain databases

    Gene3Di 3.90.550.10. 2 hits.
    InterProi IPR002654. Glyco_trans_25.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF01755. Glyco_transf_25. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
      Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
      , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
      DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Teratocarcinoma.
    2. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Cervix.
    5. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
      J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-96 AND ASN-381.
      Tissue: Liver.
    6. "Core glycosylation of collagen is initiated by two beta(1-O)galactosyltransferases."
      Schegg B., Huelsmeier A.J., Rutschmann C., Maag C., Hennet T.
      Mol. Cell. Biol. 29:943-952(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
    7. "The human collagen beta(1-O)galactosyltransferase, GLT25D1, is a soluble endoplasmic reticulum localized protein."
      Liefhebber J.M., Punt S., Spaan W.J., van Leeuwen H.C.
      BMC Cell Biol. 11:33-33(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, IDENTIFICATION BY MASS SPECTROMETRY.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGT251_HUMAN
    AccessioniPrimary (citable) accession number: Q8NBJ5
    Secondary accession number(s): Q8NC64
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 4, 2007
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    Has no glucosyltransferase activity.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3