Q8NBJ4 (GOLM1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Golgi membrane protein 1

Alternative name(s):
Golgi membrane protein GP73
Golgi phosphoprotein 2

Gene names

Name:	GOLM1
Synonyms:	C9orf155, GOLPH2
ORF Names:	PSEC0242, UNQ686/PRO1326

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Unknown. Cellular response protein to viral infection.
Subunit structure	Interacts with DYM. Ref.11
Subcellular location	Golgi apparatus › cis-Golgi network membrane; Single-pass type II membrane protein. Note: Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption. Ref.1 Ref.6
Tissue specificity	Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocytes-derived syncytial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes. Ref.1 Ref.7
Induction	Up-regulated in response to viral infection. Induced by the E1A adenoviral protein. Ref.8
Post-translational modification	Glycosylated. Ref.1 Phosphorylation sites are present in the extracellular medium.
Sequence similarities	Belongs to the GOLM1/CASC4 family.
Caution	It is uncertain whether Met-1 or Met-2 is the initiator.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative initiation Polymorphism
Domain	Coiled coil Signal-anchor Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi apparatus Inferred from direct assay. Source: HPA integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform 1 (identifier: Q8NBJ4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NBJ4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Golgi membrane protein 1		PRO_0000021356
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 35	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	36 – 401	366	Lumenal Potential
	Coiled coil	40 – 205	166	Potential
Amino acid modifications
	Glycosylation	109	1	N-linked (GlcNAc...) Ref.9 Ref.10
	Glycosylation	144	1	N-linked (GlcNAc...) Potential
	Glycosylation	398	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 10	10	Missing in isoform 2.		VSP_018782
	Natural variant	217	1	H → R. Corresponds to variant rs2297002 [ dbSNP | Ensembl ].		VAR_053922
Experimental info
	Sequence conflict	249	1	Q → R in CAG33482. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 24C1DC31E47B5B77	FASTA	401	45,333
	10 20 30 40 50 60 MMGLGNGRRS MKSPPLVLAA LVACIIVLGF NYWIASSRSV DLQTRIMELE GRVRRAAAER 70 80 90 100 110 120 GAVELKKNEF QGELEKQREQ LDKIQSSHNF QLESVNKLYQ DEKAVLVNNI TTGERLIRVL 130 140 150 160 170 180 QDQLKTLQRN YGRLQQDVLQ FQKNQTNLER KFSYDLSQCI NQMKEVKEQC EERIEEVTKK 190 200 210 220 230 240 GNEAVASRDL SENNDQRQQL QALSEPQPRL QAAGLPHTEV PQGKGNVLGN SKSQTPAPSS 250 260 270 280 290 300 EVVLDSKRQV EKEETNEIQV VNEEPQRDRL PQEPGREQVV EDRPVGGRGF GGAGELGQTP 310 320 330 340 350 360 QVQAALSVSQ ENPEMEGPER DQLVIPDGQE EEQEAAGEGR NQQKLRGEDD YNMDENEAES 370 380 390 400 ETDKQAALAG NDRNIDVFNV EDQKRDTINL LDQREKRNHT L
	Isoform 2 [UniParc]. Checksum: 5BCF2B6097DFA458	FASTA	391	44,273
	10 20 30 40 50 60 MKSPPLVLAA LVACIIVLGF NYWIASSRSV DLQTRIMELE GRVRRAAAER GAVELKKNEF 70 80 90 100 110 120 QGELEKQREQ LDKIQSSHNF QLESVNKLYQ DEKAVLVNNI TTGERLIRVL QDQLKTLQRN 130 140 150 160 170 180 YGRLQQDVLQ FQKNQTNLER KFSYDLSQCI NQMKEVKEQC EERIEEVTKK GNEAVASRDL 190 200 210 220 230 240 SENNDQRQQL QALSEPQPRL QAAGLPHTEV PQGKGNVLGN SKSQTPAPSS EVVLDSKRQV 250 260 270 280 290 300 EKEETNEIQV VNEEPQRDRL PQEPGREQVV EDRPVGGRGF GGAGELGQTP QVQAALSVSQ 310 320 330 340 350 360 ENPEMEGPER DQLVIPDGQE EEQEAAGEGR NQQKLRGEDD YNMDENEAES ETDKQAALAG 370 380 390 NDRNIDVFNV EDQKRDTINL LDQREKRNHT L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GP73, a novel Golgi-localized protein upregulated by viral infection." Kladney R.D., Bulla G.A., Guo L., Mason A.L., Tollefson A.E., Simon D.J., Koutoubi Z., Fimmel C.J. Gene 249:53-65(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE INITIATION, GLYCOSYLATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Tissue: Liver.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Teratocarcinoma.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[6]	"Cycling of early Golgi proteins via the cell surface and endosomes upon lumenal pH disruption." Puri S., Bachert C., Fimmel C.J., Linstedt A.D. Traffic 3:641-653(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Expression of GP73, a resident Golgi membrane protein, in viral and nonviral liver disease." Kladney R.D., Cui X., Bulla G.A., Brunt E.M., Fimmel C.J. Hepatology 35:1431-1440(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Upregulation of the Golgi protein GP73 by adenovirus infection requires the E1A CtBP interaction domain." Kladney R.D., Tollefson A.E., Wold W.S., Fimmel C.J. Virology 301:236-246(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY VIRAL INFECTION.
[9]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-109, MASS SPECTROMETRY. Tissue: Plasma.
[10]	"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry." Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A. J. Proteome Res. 5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-109, MASS SPECTROMETRY. Tissue: Saliva.
[11]	"Dymeclin, the gene underlying Dyggve-Melchior-Clausen syndrome, encodes a protein integral to extracellular matrix and Golgi organization and is associated with protein secretion pathways critical in bone development." Denais C., Dent C.L., Southgate L., Hoyle J., Dafou D., Trembath R.C., Machado R.D. Hum. Mutat. 32:231-239(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DYM.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF236056 mRNA. Translation: AAF44663.1. AY358593 mRNA. Translation: AAQ88956.1. CR457201 mRNA. Translation: CAG33482.1. AK075542 mRNA. Translation: BAC11685.1. BC001740 mRNA. Translation: AAH01740.1.
IPI	IPI00171411. IPI00759659.
RefSeq	NP_057632.2. NM_016548.3. NP_808800.1. NM_177937.2.
UniGene	Hs.494337.
3D structure databases
ProteinModelPortal	Q8NBJ4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8NBJ4. 26 interactions.
MINT	MINT-1378675.
PTM databases
PhosphoSite	Q8NBJ4.
Polymorphism databases
DMDM	51316027.
Proteomic databases
PaxDb	Q8NBJ4.
PRIDE	Q8NBJ4.
Protocols and materials databases
DNASU	51280.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000388711; ENSP00000373363; ENSG00000135052. ENST00000388712; ENSP00000373364; ENSG00000135052.
GeneID	51280.
KEGG	hsa:51280.
UCSC	uc004aol.3. human.
Organism-specific databases
CTD	51280.
GeneCards	GC09M088641.
H-InvDB	HIX0153288.
HGNC	HGNC:15451. GOLM1.
HPA	CAB015341. HPA010638. HPA011929.
MIM	606804. gene.
neXtProt	NX_Q8NBJ4.
PharmGKB	PA28811.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG39658.
HOGENOM	HOG000065682.
HOVERGEN	HBG062332.
OMA	NAENQKG.
OrthoDB	EOG4ZKJNH.
Gene expression databases
ArrayExpress	Q8NBJ4.
Bgee	Q8NBJ4.
CleanEx	HS_GOLM1.
Genevestigator	Q8NBJ4.
GermOnline	ENSG00000135052. Homo sapiens.
Family and domain databases
InterPro	IPR026143. GOLM1. IPR026139. GOLM1/CASC4. [Graphical view]
PANTHER	PTHR15896. PTHR15896. 1 hit. PTHR15896:SF5. PTHR15896:SF5. 1 hit.
PRINTS	PR02084. GOLM1CASC4.
ProtoNet	Search...
Other
ChiTaRS	GOLM1. human.
GenomeRNAi	51280.
NextBio	54505.
SOURCE	Search...

Entry information

Entry name

GOLM1_HUMAN

Accession

Primary (citable) accession number: Q8NBJ4
Secondary accession number(s): Q6IAF4, Q9NRB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	October 1, 2002
Last modified:	April 3, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.