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Q8NBJ4 (GOLM1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Golgi membrane protein 1
Alternative name(s):
Golgi membrane protein GP73
Golgi phosphoprotein 2
Gene names
Name:GOLM1
Synonyms:C9orf155, GOLPH2
ORF Names:PSEC0242, UNQ686/PRO1326
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Unknown. Cellular response protein to viral infection.

Subunit structure

Interacts with DYM. Ref.12

Subcellular location

Golgi apparatuscis-Golgi network membrane; Single-pass type II membrane protein. Note: Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption. Ref.1 Ref.6

Tissue specificity

Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocytes-derived syncytial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes. Ref.1 Ref.7

Induction

Up-regulated in response to viral infection. Induced by the E1A adenoviral protein. Ref.8

Post-translational modification

Glycosylated. Ref.1 Ref.11

Phosphorylation sites are present in the extracellular medium.

Sequence similarities

Belongs to the GOLM1/CASC4 family.

Caution

It is uncertain whether Met-1 or Met-2 is the initiator.

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform 1 (identifier: Q8NBJ4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8NBJ4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 401401Golgi membrane protein 1
PRO_0000021356

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3523Helical; Signal-anchor for type II membrane protein; Potential
Topological domain36 – 401366Lumenal Potential
Coiled coil40 – 205166 Potential

Amino acid modifications

Glycosylation1091N-linked (GlcNAc...) (complex) Ref.9 Ref.10 Ref.11
Glycosylation1441N-linked (GlcNAc...) Potential
Glycosylation3981N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 1010Missing in isoform 2.
VSP_018782
Natural variant2171H → R.
Corresponds to variant rs2297002 [ dbSNP | Ensembl ].
VAR_053922

Experimental info

Sequence conflict2491Q → R in CAG33482. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 24C1DC31E47B5B77

FASTA40145,333
        10         20         30         40         50         60 
MMGLGNGRRS MKSPPLVLAA LVACIIVLGF NYWIASSRSV DLQTRIMELE GRVRRAAAER 

        70         80         90        100        110        120 
GAVELKKNEF QGELEKQREQ LDKIQSSHNF QLESVNKLYQ DEKAVLVNNI TTGERLIRVL 

       130        140        150        160        170        180 
QDQLKTLQRN YGRLQQDVLQ FQKNQTNLER KFSYDLSQCI NQMKEVKEQC EERIEEVTKK 

       190        200        210        220        230        240 
GNEAVASRDL SENNDQRQQL QALSEPQPRL QAAGLPHTEV PQGKGNVLGN SKSQTPAPSS 

       250        260        270        280        290        300 
EVVLDSKRQV EKEETNEIQV VNEEPQRDRL PQEPGREQVV EDRPVGGRGF GGAGELGQTP 

       310        320        330        340        350        360 
QVQAALSVSQ ENPEMEGPER DQLVIPDGQE EEQEAAGEGR NQQKLRGEDD YNMDENEAES 

       370        380        390        400 
ETDKQAALAG NDRNIDVFNV EDQKRDTINL LDQREKRNHT L 

« Hide

Isoform 2 [UniParc].

Checksum: 5BCF2B6097DFA458
Show »

FASTA39144,273

References

« Hide 'large scale' references
[1]"GP73, a novel Golgi-localized protein upregulated by viral infection."
Kladney R.D., Bulla G.A., Guo L., Mason A.L., Tollefson A.E., Simon D.J., Koutoubi Z., Fimmel C.J.
Gene 249:53-65(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE INITIATION, GLYCOSYLATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
Tissue: Liver.
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. expand/collapse author list , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Teratocarcinoma.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[6]"Cycling of early Golgi proteins via the cell surface and endosomes upon lumenal pH disruption."
Puri S., Bachert C., Fimmel C.J., Linstedt A.D.
Traffic 3:641-653(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"Expression of GP73, a resident Golgi membrane protein, in viral and nonviral liver disease."
Kladney R.D., Cui X., Bulla G.A., Brunt E.M., Fimmel C.J.
Hepatology 35:1431-1440(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[8]"Upregulation of the Golgi protein GP73 by adenovirus infection requires the E1A CtBP interaction domain."
Kladney R.D., Tollefson A.E., Wold W.S., Fimmel C.J.
Virology 301:236-246(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY VIRAL INFECTION.
[9]"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-109.
Tissue: Plasma.
[10]"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A.
J. Proteome Res. 5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-109.
Tissue: Saliva.
[11]"A strategy for precise and large scale identification of core fucosylated glycoproteins."
Jia W., Lu Z., Fu Y., Wang H.P., Wang L.H., Chi H., Yuan Z.F., Zheng Z.B., Song L.N., Han H.H., Liang Y.M., Wang J.L., Cai Y., Zhang Y.K., Deng Y.L., Ying W.T., He S.M., Qian X.H.
Mol. Cell. Proteomics 8:913-923(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT ASN-109.
[12]"Dymeclin, the gene underlying Dyggve-Melchior-Clausen syndrome, encodes a protein integral to extracellular matrix and Golgi organization and is associated with protein secretion pathways critical in bone development."
Denais C., Dent C.L., Southgate L., Hoyle J., Dafou D., Trembath R.C., Machado R.D.
Hum. Mutat. 32:231-239(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DYM.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF236056 mRNA. Translation: AAF44663.1.
AY358593 mRNA. Translation: AAQ88956.1.
CR457201 mRNA. Translation: CAG33482.1.
AK075542 mRNA. Translation: BAC11685.1.
BC001740 mRNA. Translation: AAH01740.1.
CCDSCCDS35054.1. [Q8NBJ4-1]
RefSeqNP_057632.2. NM_016548.3. [Q8NBJ4-1]
NP_808800.1. NM_177937.2. [Q8NBJ4-1]
UniGeneHs.494337.

3D structure databases

ProteinModelPortalQ8NBJ4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119432. 13 interactions.
IntActQ8NBJ4. 27 interactions.
MINTMINT-1378675.

PTM databases

PhosphoSiteQ8NBJ4.

Polymorphism databases

DMDM51316027.

Proteomic databases

MaxQBQ8NBJ4.
PaxDbQ8NBJ4.
PRIDEQ8NBJ4.

Protocols and materials databases

DNASU51280.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000388711; ENSP00000373363; ENSG00000135052. [Q8NBJ4-1]
ENST00000388712; ENSP00000373364; ENSG00000135052. [Q8NBJ4-1]
GeneID51280.
KEGGhsa:51280.
UCSCuc004aol.3. human. [Q8NBJ4-1]

Organism-specific databases

CTD51280.
GeneCardsGC09M088641.
H-InvDBHIX0153288.
HGNCHGNC:15451. GOLM1.
HPACAB015341.
HPA010638.
HPA011929.
MIM606804. gene.
neXtProtNX_Q8NBJ4.
PharmGKBPA28811.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG39658.
HOGENOMHOG000065682.
HOVERGENHBG062332.
OMAKFSYDLN.
OrthoDBEOG7992QP.
PhylomeDBQ8NBJ4.
TreeFamTF331127.

Gene expression databases

ArrayExpressQ8NBJ4.
BgeeQ8NBJ4.
CleanExHS_GOLM1.
GenevestigatorQ8NBJ4.

Family and domain databases

InterProIPR026143. GOLM1.
IPR026139. GOLM1/CASC4.
[Graphical view]
PANTHERPTHR15896. PTHR15896. 1 hit.
PTHR15896:SF8. PTHR15896:SF8. 1 hit.
PRINTSPR02084. GOLM1CASC4.
ProtoNetSearch...

Other

ChiTaRSGOLM1. human.
GeneWikiGOLM1.
GenomeRNAi51280.
NextBio54505.
PROQ8NBJ4.
SOURCESearch...

Entry information

Entry nameGOLM1_HUMAN
AccessionPrimary (citable) accession number: Q8NBJ4
Secondary accession number(s): Q6IAF4, Q9NRB9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM