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Protein

Cytochrome b ascorbate-dependent protein 3

Gene

CYB561A3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ferric-chelate reductase that reduces Fe3+ to Fe2+ before its transport from the endosome to the cytoplasm. Probably uses ascorbate as electron donor (By similarity).By similarity

Cofactori

hemeBy similarityNote: Binds 2 heme groups non-covalently.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi83Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi87Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi105Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi117Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi156Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
LigandHeme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b ascorbate-dependent protein 3 (EC:1.-.-.-)
Alternative name(s):
Cytochrome b561 family member A3
Lysosomal cytochrome b
Short name:
LCytb
Gene namesi
Name:CYB561A3
Synonyms:CYBASC3, LCYTB
ORF Names:PSEC0259
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000162144.9
HGNCiHGNC:23014 CYB561A3
neXtProtiNX_Q8NBI2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei8 – 28Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei46 – 66Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei84 – 104Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei120 – 140Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei155 – 175Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei203 – 223Helical; Name=6Sequence analysisAdd BLAST21

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000162144
PharmGKBiPA134880796

Polymorphism and mutation databases

BioMutaiCYB561A3
DMDMi74730147

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003148381 – 242Cytochrome b ascorbate-dependent protein 3Add BLAST242

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8NBI2
PaxDbiQ8NBI2
PeptideAtlasiQ8NBI2
PRIDEiQ8NBI2
ProteomicsDBi72770

Expressioni

Gene expression databases

BgeeiENSG00000162144
CleanExiHS_CYBASC3
ExpressionAtlasiQ8NBI2 baseline and differential
GenevisibleiQ8NBI2 HS

Organism-specific databases

HPAiHPA052548

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi128617, 2 interactors
IntActiQ8NBI2, 5 interactors
STRINGi9606.ENSP00000398979

Structurei

3D structure databases

ProteinModelPortaliQ8NBI2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 219Cytochrome b561PROSITE-ProRule annotationAdd BLAST208

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1619 Eukaryota
ENOG4111FKI LUCA
GeneTreeiENSGT00390000010986
HOGENOMiHOG000231043
HOVERGENiHBG054164
InParanoidiQ8NBI2
KOiK16295
PhylomeDBiQ8NBI2
TreeFamiTF314222

Family and domain databases

InterProiView protein in InterPro
IPR028836 Cyb561a3
IPR006593 Cyt_b561/ferric_Rdtase_TM
PANTHERiPTHR10106:SF0 PTHR10106:SF0, 1 hit
PfamiView protein in Pfam
PF03188 Cytochrom_B561, 1 hit
SMARTiView protein in SMART
SM00665 B561, 1 hit
PROSITEiView protein in PROSITE
PS50939 CYTOCHROME_B561, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NBI2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSGRFYLSC LLLGSLGSMC ILFTIYWMQY WRGGFAWNGS IYMFNWHPVL
60 70 80 90 100
MVAGMVVFYG GASLVYRLPQ SWVGPKLPWK LLHAALHLMA FVLTVVGLVA
110 120 130 140 150
VFTFHNHGRT ANLYSLHSWL GITTVFLFAC QWFLGFAVFL LPWASMWLRS
160 170 180 190 200
LLKPIHVFFG AAILSLSIAS VISGINEKLF FSLKNTTRPY HSLPSEAVFA
210 220 230 240
NSTGMLVVAF GLLVLYILLA SSWKRPEPGI LTDRQPLLHD GE
Length:242
Mass (Da):27,214
Last modified:October 1, 2002 - v1
Checksum:i1D0C4904CEAED747
GO
Isoform 2 (identifier: Q8NBI2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGKNFSDSFLSRECVIRM

Show »
Length:259
Mass (Da):29,186
Checksum:i426A35886A289F17
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti122I → T in AAH04391 (PubMed:15489334).Curated1
Sequence conflicti168I → N in BAG59601 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0473581M → MGKNFSDSFLSRECVIRM in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056751 mRNA Translation: BAG51804.1
AK075559 mRNA Translation: BAC11698.1
AK297084 mRNA Translation: BAG59601.1
AP003108 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW73941.1
CH471076 Genomic DNA Translation: EAW73942.1
BC004391 mRNA Translation: AAH04391.1
BC014045 mRNA Translation: AAH14045.2
BC047710 mRNA Translation: AAH47710.1
CCDSiCCDS53639.1 [Q8NBI2-2]
CCDS8004.1 [Q8NBI2-1]
RefSeqiNP_001154924.1, NM_001161452.1 [Q8NBI2-1]
NP_001154926.1, NM_001161454.1 [Q8NBI2-2]
NP_001287692.1, NM_001300763.1
NP_705839.3, NM_153611.4 [Q8NBI2-1]
UniGeneiHs.22546

Genome annotation databases

EnsembliENST00000294072; ENSP00000294072; ENSG00000162144 [Q8NBI2-1]
ENST00000426130; ENSP00000398979; ENSG00000162144 [Q8NBI2-2]
ENST00000536915; ENSP00000437390; ENSG00000162144 [Q8NBI2-1]
GeneIDi220002
KEGGihsa:220002
UCSCiuc001nrg.4 human [Q8NBI2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCYAC3_HUMAN
AccessioniPrimary (citable) accession number: Q8NBI2
Secondary accession number(s): B3KPU2
, B4DLN9, J3KQH4, Q6PK96
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2002
Last modified: June 20, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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