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Q8NBI2 (CYAC3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome b ascorbate-dependent protein 3

EC=1.-.-.-
Alternative name(s):
Cytochrome b561 family member A3
Lysosomal cytochrome b
Short name=LCytb
Gene names
Name:CYB561A3
Synonyms:CYBASC3, LCYTB
ORF Names:PSEC0259
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length242 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Ferric-chelate reductase that reduces Fe3+ to Fe2+ before its transport from the endosome to the cytoplasm. Probably uses ascorbate as electron donor By similarity.

Cofactor

Binds 2 heme groups non-covalently By similarity.

Subcellular location

Late endosome membrane; Multi-pass membrane protein By similarity. Lysosome membrane; Multi-pass membrane protein Ref.6.

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Contains 1 cytochrome b561 domain.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8NBI2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8NBI2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGKNFSDSFLSRECVIRM

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 242242Cytochrome b ascorbate-dependent protein 3
PRO_0000314838

Regions

Transmembrane8 – 2821Helical; Name=1; Potential
Transmembrane46 – 6621Helical; Name=2; Potential
Transmembrane84 – 10421Helical; Name=3; Potential
Transmembrane120 – 14021Helical; Name=4; Potential
Transmembrane155 – 17521Helical; Name=5; Potential
Transmembrane203 – 22321Helical; Name=6; Potential
Domain12 – 219208Cytochrome b561

Sites

Metal binding471Iron (heme axial ligand) Potential
Metal binding831Iron (heme axial ligand) Potential
Metal binding871Iron (heme axial ligand) Potential
Metal binding1051Iron (heme axial ligand) Potential
Metal binding1171Iron (heme axial ligand) Potential
Metal binding1561Iron (heme axial ligand) Potential

Amino acid modifications

Glycosylation381N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence11M → MGKNFSDSFLSRECVIRM in isoform 2.
VSP_047358

Experimental info

Sequence conflict1221I → T in AAH04391. Ref.5
Sequence conflict1681I → N in BAG59601. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 1D0C4904CEAED747

FASTA24227,214
        10         20         30         40         50         60 
MVSGRFYLSC LLLGSLGSMC ILFTIYWMQY WRGGFAWNGS IYMFNWHPVL MVAGMVVFYG 

        70         80         90        100        110        120 
GASLVYRLPQ SWVGPKLPWK LLHAALHLMA FVLTVVGLVA VFTFHNHGRT ANLYSLHSWL 

       130        140        150        160        170        180 
GITTVFLFAC QWFLGFAVFL LPWASMWLRS LLKPIHVFFG AAILSLSIAS VISGINEKLF 

       190        200        210        220        230        240 
FSLKNTTRPY HSLPSEAVFA NSTGMLVVAF GLLVLYILLA SSWKRPEPGI LTDRQPLLHD 


GE 

« Hide

Isoform 2 [UniParc].

Checksum: 426A35886A289F17
Show »

FASTA25929,186

References

[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Blood and Placenta.
[2]"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. expand/collapse author list , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain, Lymph and Pancreas.
[6]"Integral and associated lysosomal membrane proteins."
Schroeder B., Wrocklage C., Pan C., Jaeger R., Koesters B., Schaefer H., Elsaesser H.-P., Mann M., Hasilik A.
Traffic 8:1676-1686(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Placenta.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK056751 mRNA. Translation: BAG51804.1.
AK075559 mRNA. Translation: BAC11698.1.
AK297084 mRNA. Translation: BAG59601.1.
AP003108 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73941.1.
CH471076 Genomic DNA. Translation: EAW73942.1.
BC004391 mRNA. Translation: AAH04391.1.
BC014045 mRNA. Translation: AAH14045.2.
BC047710 mRNA. Translation: AAH47710.1.
RefSeqNP_001154924.1. NM_001161452.1.
NP_001154926.1. NM_001161454.1.
NP_705839.3. NM_153611.4.
UniGeneHs.22546.

3D structure databases

ProteinModelPortalQ8NBI2.
ModBaseSearch...
MobiDBSearch...

Polymorphism databases

DMDM74730147.

Proteomic databases

PRIDEQ8NBI2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000294072; ENSP00000294072; ENSG00000162144. [Q8NBI2-1]
ENST00000426130; ENSP00000398979; ENSG00000162144. [Q8NBI2-2]
ENST00000536915; ENSP00000437390; ENSG00000162144. [Q8NBI2-1]
GeneID220002.
KEGGhsa:220002.
UCSCuc001nrf.4. human. [Q8NBI2-1]

Organism-specific databases

CTD220002.
GeneCardsGC11M061117.
HGNCHGNC:23014. CYB561A3.
HPAHPA052548.
neXtProtNX_Q8NBI2.
PharmGKBPA134880796.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG327154.
HOGENOMHOG000231043.
HOVERGENHBG054164.
InParanoidQ8NBI2.
KOK16295.
OrthoDBEOG7SJD5W.
PhylomeDBQ8NBI2.
TreeFamTF314222.

Gene expression databases

ArrayExpressQ8NBI2.
BgeeQ8NBI2.
CleanExHS_CYBASC3.
GenevestigatorQ8NBI2.

Family and domain databases

InterProIPR028836. Cyb561a3.
IPR006593. Cyt_b561/ferric_Rdtase_TM.
IPR004877. Cyt_b561_euk.
[Graphical view]
PANTHERPTHR10106:SF2. PTHR10106:SF2. 1 hit.
PfamPF03188. Cytochrom_B561. 1 hit.
[Graphical view]
SMARTSM00665. B561. 1 hit.
[Graphical view]
PROSITEPS50939. CYTOCHROME_B561. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCYBASC3. human.
GenomeRNAi220002.
NextBio35535341.
PROQ8NBI2.

Entry information

Entry nameCYAC3_HUMAN
AccessionPrimary (citable) accession number: Q8NBI2
Secondary accession number(s): B3KPU2 expand/collapse secondary AC list , B4DLN9, J3KQH4, Q6PK96
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2002
Last modified: March 19, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM