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Q8NBI2

- CYAC3_HUMAN

UniProt

Q8NBI2 - CYAC3_HUMAN

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Protein

Cytochrome b ascorbate-dependent protein 3

Gene

CYB561A3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Ferric-chelate reductase that reduces Fe3+ to Fe2+ before its transport from the endosome to the cytoplasm. Probably uses ascorbate as electron donor (By similarity).By similarity

Cofactori

hemeBy similarityNote: Binds 2 heme groups non-covalently.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi83 – 831Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi87 – 871Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi105 – 1051Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi117 – 1171Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi156 – 1561Iron (heme axial ligand)PROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. oxidoreductase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b ascorbate-dependent protein 3 (EC:1.-.-.-)
Alternative name(s):
Cytochrome b561 family member A3
Lysosomal cytochrome b
Short name:
LCytb
Gene namesi
Name:CYB561A3
Synonyms:CYBASC3, LCYTB
ORF Names:PSEC0259
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Unplaced

Organism-specific databases

HGNCiHGNC:23014. CYB561A3.

Subcellular locationi

Late endosome membrane By similarity; Multi-pass membrane protein By similarity. Lysosome membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2821Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei46 – 6621Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei84 – 10421Helical; Name=3Sequence AnalysisAdd
BLAST
Transmembranei120 – 14021Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei155 – 17521Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei203 – 22321Helical; Name=6Sequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. late endosome membrane Source: UniProtKB
  3. lysosomal membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134880796.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 242242Cytochrome b ascorbate-dependent protein 3PRO_0000314838Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8NBI2.
PRIDEiQ8NBI2.

Expressioni

Gene expression databases

BgeeiQ8NBI2.
CleanExiHS_CYBASC3.
ExpressionAtlasiQ8NBI2. baseline and differential.
GenevestigatoriQ8NBI2.

Organism-specific databases

HPAiHPA052548.

Structurei

3D structure databases

ProteinModelPortaliQ8NBI2.
SMRiQ8NBI2. Positions 16-215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 219208Cytochrome b561PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 cytochrome b561 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327154.
GeneTreeiENSGT00390000010986.
HOGENOMiHOG000231043.
HOVERGENiHBG054164.
InParanoidiQ8NBI2.
KOiK16295.
OrthoDBiEOG7SJD5W.
PhylomeDBiQ8NBI2.
TreeFamiTF314222.

Family and domain databases

InterProiIPR028836. Cyb561a3.
IPR006593. Cyt_b561/ferric_Rdtase_TM.
IPR004877. Cyt_b561_euk.
[Graphical view]
PANTHERiPTHR10106:SF2. PTHR10106:SF2. 1 hit.
PfamiPF03188. Cytochrom_B561. 1 hit.
[Graphical view]
SMARTiSM00665. B561. 1 hit.
[Graphical view]
PROSITEiPS50939. CYTOCHROME_B561. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8NBI2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSGRFYLSC LLLGSLGSMC ILFTIYWMQY WRGGFAWNGS IYMFNWHPVL
60 70 80 90 100
MVAGMVVFYG GASLVYRLPQ SWVGPKLPWK LLHAALHLMA FVLTVVGLVA
110 120 130 140 150
VFTFHNHGRT ANLYSLHSWL GITTVFLFAC QWFLGFAVFL LPWASMWLRS
160 170 180 190 200
LLKPIHVFFG AAILSLSIAS VISGINEKLF FSLKNTTRPY HSLPSEAVFA
210 220 230 240
NSTGMLVVAF GLLVLYILLA SSWKRPEPGI LTDRQPLLHD GE
Length:242
Mass (Da):27,214
Last modified:October 1, 2002 - v1
Checksum:i1D0C4904CEAED747
GO
Isoform 2 (identifier: Q8NBI2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGKNFSDSFLSRECVIRM

Show »
Length:259
Mass (Da):29,186
Checksum:i426A35886A289F17
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti122 – 1221I → T in AAH04391. (PubMed:15489334)Curated
Sequence conflicti168 – 1681I → N in BAG59601. (PubMed:14702039)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MGKNFSDSFLSRECVIRM in isoform 2. 1 PublicationVSP_047358

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056751 mRNA. Translation: BAG51804.1.
AK075559 mRNA. Translation: BAC11698.1.
AK297084 mRNA. Translation: BAG59601.1.
AP003108 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73941.1.
CH471076 Genomic DNA. Translation: EAW73942.1.
BC004391 mRNA. Translation: AAH04391.1.
BC014045 mRNA. Translation: AAH14045.2.
BC047710 mRNA. Translation: AAH47710.1.
CCDSiCCDS53639.1. [Q8NBI2-2]
CCDS8004.1. [Q8NBI2-1]
RefSeqiNP_001154924.1. NM_001161452.1. [Q8NBI2-1]
NP_001154926.1. NM_001161454.1. [Q8NBI2-2]
NP_001287692.1. NM_001300763.1.
NP_705839.3. NM_153611.4. [Q8NBI2-1]
UniGeneiHs.22546.

Genome annotation databases

EnsembliENST00000294072; ENSP00000294072; ENSG00000162144. [Q8NBI2-1]
ENST00000426130; ENSP00000398979; ENSG00000162144. [Q8NBI2-2]
ENST00000536915; ENSP00000437390; ENSG00000162144. [Q8NBI2-1]
GeneIDi220002.
KEGGihsa:220002.
UCSCiuc001nrf.4. human. [Q8NBI2-1]
uc010rlh.2. human.

Polymorphism databases

DMDMi74730147.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056751 mRNA. Translation: BAG51804.1 .
AK075559 mRNA. Translation: BAC11698.1 .
AK297084 mRNA. Translation: BAG59601.1 .
AP003108 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73941.1 .
CH471076 Genomic DNA. Translation: EAW73942.1 .
BC004391 mRNA. Translation: AAH04391.1 .
BC014045 mRNA. Translation: AAH14045.2 .
BC047710 mRNA. Translation: AAH47710.1 .
CCDSi CCDS53639.1. [Q8NBI2-2 ]
CCDS8004.1. [Q8NBI2-1 ]
RefSeqi NP_001154924.1. NM_001161452.1. [Q8NBI2-1 ]
NP_001154926.1. NM_001161454.1. [Q8NBI2-2 ]
NP_001287692.1. NM_001300763.1.
NP_705839.3. NM_153611.4. [Q8NBI2-1 ]
UniGenei Hs.22546.

3D structure databases

ProteinModelPortali Q8NBI2.
SMRi Q8NBI2. Positions 16-215.
ModBasei Search...
MobiDBi Search...

Polymorphism databases

DMDMi 74730147.

Proteomic databases

MaxQBi Q8NBI2.
PRIDEi Q8NBI2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000294072 ; ENSP00000294072 ; ENSG00000162144 . [Q8NBI2-1 ]
ENST00000426130 ; ENSP00000398979 ; ENSG00000162144 . [Q8NBI2-2 ]
ENST00000536915 ; ENSP00000437390 ; ENSG00000162144 . [Q8NBI2-1 ]
GeneIDi 220002.
KEGGi hsa:220002.
UCSCi uc001nrf.4. human. [Q8NBI2-1 ]
uc010rlh.2. human.

Organism-specific databases

CTDi 220002.
GeneCardsi GC11M061117.
HGNCi HGNC:23014. CYB561A3.
HPAi HPA052548.
neXtProti NX_Q8NBI2.
PharmGKBi PA134880796.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG327154.
GeneTreei ENSGT00390000010986.
HOGENOMi HOG000231043.
HOVERGENi HBG054164.
InParanoidi Q8NBI2.
KOi K16295.
OrthoDBi EOG7SJD5W.
PhylomeDBi Q8NBI2.
TreeFami TF314222.

Miscellaneous databases

ChiTaRSi CYB561A3. human.
GenomeRNAii 220002.
NextBioi 35535341.
PROi Q8NBI2.

Gene expression databases

Bgeei Q8NBI2.
CleanExi HS_CYBASC3.
ExpressionAtlasi Q8NBI2. baseline and differential.
Genevestigatori Q8NBI2.

Family and domain databases

InterProi IPR028836. Cyb561a3.
IPR006593. Cyt_b561/ferric_Rdtase_TM.
IPR004877. Cyt_b561_euk.
[Graphical view ]
PANTHERi PTHR10106:SF2. PTHR10106:SF2. 1 hit.
Pfami PF03188. Cytochrom_B561. 1 hit.
[Graphical view ]
SMARTi SM00665. B561. 1 hit.
[Graphical view ]
PROSITEi PS50939. CYTOCHROME_B561. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Blood and Placenta.
  2. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
    Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
    , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
    DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain, Lymph and Pancreas.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Tissue: Placenta.

Entry informationi

Entry nameiCYAC3_HUMAN
AccessioniPrimary (citable) accession number: Q8NBI2
Secondary accession number(s): B3KPU2
, B4DLN9, J3KQH4, Q6PK96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2002
Last modified: November 26, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3