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Q8NBI2

- CYAC3_HUMAN

UniProt

Q8NBI2 - CYAC3_HUMAN

Protein

Cytochrome b ascorbate-dependent protein 3

Gene

CYB561A3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Ferric-chelate reductase that reduces Fe3+ to Fe2+ before its transport from the endosome to the cytoplasm. Probably uses ascorbate as electron donor By similarity.By similarity

    Cofactori

    Binds 2 heme groups non-covalently.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471Iron (heme axial ligand)PROSITE-ProRule annotation
    Metal bindingi83 – 831Iron (heme axial ligand)PROSITE-ProRule annotation
    Metal bindingi87 – 871Iron (heme axial ligand)PROSITE-ProRule annotation
    Metal bindingi105 – 1051Iron (heme axial ligand)PROSITE-ProRule annotation
    Metal bindingi117 – 1171Iron (heme axial ligand)PROSITE-ProRule annotation
    Metal bindingi156 – 1561Iron (heme axial ligand)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. oxidoreductase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome b ascorbate-dependent protein 3 (EC:1.-.-.-)
    Alternative name(s):
    Cytochrome b561 family member A3
    Lysosomal cytochrome b
    Short name:
    LCytb
    Gene namesi
    Name:CYB561A3
    Synonyms:CYBASC3, LCYTB
    ORF Names:PSEC0259
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:23014. CYB561A3.

    Subcellular locationi

    Late endosome membrane By similarity; Multi-pass membrane protein By similarity. Lysosome membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. late endosome membrane Source: UniProtKB
    3. lysosomal membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endosome, Lysosome, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134880796.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 242242Cytochrome b ascorbate-dependent protein 3PRO_0000314838Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ8NBI2.
    PRIDEiQ8NBI2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8NBI2.
    BgeeiQ8NBI2.
    CleanExiHS_CYBASC3.
    GenevestigatoriQ8NBI2.

    Organism-specific databases

    HPAiHPA052548.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NBI2.
    SMRiQ8NBI2. Positions 16-215.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2821Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei46 – 6621Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei84 – 10421Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei120 – 14021Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei155 – 17521Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei203 – 22321Helical; Name=6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 219208Cytochrome b561PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 cytochrome b561 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG327154.
    HOGENOMiHOG000231043.
    HOVERGENiHBG054164.
    InParanoidiQ8NBI2.
    KOiK16295.
    OrthoDBiEOG7SJD5W.
    PhylomeDBiQ8NBI2.
    TreeFamiTF314222.

    Family and domain databases

    InterProiIPR028836. Cyb561a3.
    IPR006593. Cyt_b561/ferric_Rdtase_TM.
    IPR004877. Cyt_b561_euk.
    [Graphical view]
    PANTHERiPTHR10106:SF2. PTHR10106:SF2. 1 hit.
    PfamiPF03188. Cytochrom_B561. 1 hit.
    [Graphical view]
    SMARTiSM00665. B561. 1 hit.
    [Graphical view]
    PROSITEiPS50939. CYTOCHROME_B561. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8NBI2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVSGRFYLSC LLLGSLGSMC ILFTIYWMQY WRGGFAWNGS IYMFNWHPVL    50
    MVAGMVVFYG GASLVYRLPQ SWVGPKLPWK LLHAALHLMA FVLTVVGLVA 100
    VFTFHNHGRT ANLYSLHSWL GITTVFLFAC QWFLGFAVFL LPWASMWLRS 150
    LLKPIHVFFG AAILSLSIAS VISGINEKLF FSLKNTTRPY HSLPSEAVFA 200
    NSTGMLVVAF GLLVLYILLA SSWKRPEPGI LTDRQPLLHD GE 242
    Length:242
    Mass (Da):27,214
    Last modified:October 1, 2002 - v1
    Checksum:i1D0C4904CEAED747
    GO
    Isoform 2 (identifier: Q8NBI2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MGKNFSDSFLSRECVIRM

    Show »
    Length:259
    Mass (Da):29,186
    Checksum:i426A35886A289F17
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti122 – 1221I → T in AAH04391. (PubMed:15489334)Curated
    Sequence conflicti168 – 1681I → N in BAG59601. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MGKNFSDSFLSRECVIRM in isoform 2. 1 PublicationVSP_047358

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK056751 mRNA. Translation: BAG51804.1.
    AK075559 mRNA. Translation: BAC11698.1.
    AK297084 mRNA. Translation: BAG59601.1.
    AP003108 Genomic DNA. No translation available.
    CH471076 Genomic DNA. Translation: EAW73941.1.
    CH471076 Genomic DNA. Translation: EAW73942.1.
    BC004391 mRNA. Translation: AAH04391.1.
    BC014045 mRNA. Translation: AAH14045.2.
    BC047710 mRNA. Translation: AAH47710.1.
    CCDSiCCDS53639.1. [Q8NBI2-2]
    CCDS8004.1. [Q8NBI2-1]
    RefSeqiNP_001154924.1. NM_001161452.1. [Q8NBI2-1]
    NP_001154926.1. NM_001161454.1. [Q8NBI2-2]
    NP_705839.3. NM_153611.4. [Q8NBI2-1]
    UniGeneiHs.22546.

    Genome annotation databases

    GeneIDi220002.
    KEGGihsa:220002.
    UCSCiuc001nrf.4. human. [Q8NBI2-1]
    uc010rlh.2. human.

    Polymorphism databases

    DMDMi74730147.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK056751 mRNA. Translation: BAG51804.1 .
    AK075559 mRNA. Translation: BAC11698.1 .
    AK297084 mRNA. Translation: BAG59601.1 .
    AP003108 Genomic DNA. No translation available.
    CH471076 Genomic DNA. Translation: EAW73941.1 .
    CH471076 Genomic DNA. Translation: EAW73942.1 .
    BC004391 mRNA. Translation: AAH04391.1 .
    BC014045 mRNA. Translation: AAH14045.2 .
    BC047710 mRNA. Translation: AAH47710.1 .
    CCDSi CCDS53639.1. [Q8NBI2-2 ]
    CCDS8004.1. [Q8NBI2-1 ]
    RefSeqi NP_001154924.1. NM_001161452.1. [Q8NBI2-1 ]
    NP_001154926.1. NM_001161454.1. [Q8NBI2-2 ]
    NP_705839.3. NM_153611.4. [Q8NBI2-1 ]
    UniGenei Hs.22546.

    3D structure databases

    ProteinModelPortali Q8NBI2.
    SMRi Q8NBI2. Positions 16-215.
    ModBasei Search...
    MobiDBi Search...

    Polymorphism databases

    DMDMi 74730147.

    Proteomic databases

    MaxQBi Q8NBI2.
    PRIDEi Q8NBI2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 220002.
    KEGGi hsa:220002.
    UCSCi uc001nrf.4. human. [Q8NBI2-1 ]
    uc010rlh.2. human.

    Organism-specific databases

    CTDi 220002.
    GeneCardsi GC11M061117.
    HGNCi HGNC:23014. CYB561A3.
    HPAi HPA052548.
    neXtProti NX_Q8NBI2.
    PharmGKBi PA134880796.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG327154.
    HOGENOMi HOG000231043.
    HOVERGENi HBG054164.
    InParanoidi Q8NBI2.
    KOi K16295.
    OrthoDBi EOG7SJD5W.
    PhylomeDBi Q8NBI2.
    TreeFami TF314222.

    Miscellaneous databases

    ChiTaRSi CYBASC3. human.
    GenomeRNAii 220002.
    NextBioi 35535341.
    PROi Q8NBI2.

    Gene expression databases

    ArrayExpressi Q8NBI2.
    Bgeei Q8NBI2.
    CleanExi HS_CYBASC3.
    Genevestigatori Q8NBI2.

    Family and domain databases

    InterProi IPR028836. Cyb561a3.
    IPR006593. Cyt_b561/ferric_Rdtase_TM.
    IPR004877. Cyt_b561_euk.
    [Graphical view ]
    PANTHERi PTHR10106:SF2. PTHR10106:SF2. 1 hit.
    Pfami PF03188. Cytochrom_B561. 1 hit.
    [Graphical view ]
    SMARTi SM00665. B561. 1 hit.
    [Graphical view ]
    PROSITEi PS50939. CYTOCHROME_B561. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Blood and Placenta.
    2. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
      Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
      , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
      DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain, Lymph and Pancreas.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Tissue: Placenta.

    Entry informationi

    Entry nameiCYAC3_HUMAN
    AccessioniPrimary (citable) accession number: Q8NBI2
    Secondary accession number(s): B3KPU2
    , B4DLN9, J3KQH4, Q6PK96
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3