Q8NBI2 (CYAC3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b ascorbate-dependent protein 3
EC=1.-.-.-

Alternative name(s):
Lysosomal cytochrome b
Short name=LCytb

Gene names

Name:	CYBASC3
Synonyms:	LCYTB
ORF Names:	PSEC0259

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ferric-chelate reductase that reduces Fe3+ to Fe2+ before its transport from the endosome to the cytoplasm. Probably uses ascorbate as electron donor By similarity.
Cofactor	Binds 2 heme groups non-covalently By similarity.
Subcellular location	Late endosome membrane; Multi-pass membrane protein By similarity. Lysosome membrane; Multi-pass membrane protein Ref.5.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Contains 1 cytochrome b561 domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Endosome Lysosome Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW late endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Cytochrome b ascorbate-dependent protein 3		PRO_0000314838
Regions
	Transmembrane	8 – 28	21	Helical; Name=1; Potential
	Transmembrane	46 – 66	21	Helical; Name=2; Potential
	Transmembrane	84 – 104	21	Helical; Name=3; Potential
	Transmembrane	120 – 140	21	Helical; Name=4; Potential
	Transmembrane	155 – 175	21	Helical; Name=5; Potential
	Transmembrane	203 – 223	21	Helical; Name=6; Potential
	Domain	12 – 219	208	Cytochrome b561
Sites
	Metal binding	47	1	Iron (heme axial ligand) Potential
	Metal binding	83	1	Iron (heme axial ligand) Potential
	Metal binding	87	1	Iron (heme axial ligand) Potential
	Metal binding	105	1	Iron (heme axial ligand) Potential
	Metal binding	117	1	Iron (heme axial ligand) Potential
	Metal binding	156	1	Iron (heme axial ligand) Potential
Amino acid modifications
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	122	1	I → T in AAH04391. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8NBI2 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 1D0C4904CEAED747	FASTA	242	27,214
	10 20 30 40 50 60 MVSGRFYLSC LLLGSLGSMC ILFTIYWMQY WRGGFAWNGS IYMFNWHPVL MVAGMVVFYG 70 80 90 100 110 120 GASLVYRLPQ SWVGPKLPWK LLHAALHLMA FVLTVVGLVA VFTFHNHGRT ANLYSLHSWL 130 140 150 160 170 180 GITTVFLFAC QWFLGFAVFL LPWASMWLRS LLKPIHVFFG AAILSLSIAS VISGINEKLF 190 200 210 220 230 240 FSLKNTTRPY HSLPSEAVFA NSTGMLVVAF GLLVLYILLA SSWKRPEPGI LTDRQPLLHD GE

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[2]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Lymph and Pancreas.
[5]	"Integral and associated lysosomal membrane proteins." Schroeder B., Wrocklage C., Pan C., Jaeger R., Koesters B., Schaefer H., Elsaesser H.-P., Mann M., Hasilik A. Traffic 8:1676-1686(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Placenta.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK056751 mRNA. Translation: BAG51804.1. AK075559 mRNA. Translation: BAC11698.1. CH471076 Genomic DNA. Translation: EAW73941.1. BC004391 mRNA. Translation: AAH04391.1. BC014045 mRNA. Translation: AAH14045.2. BC047710 mRNA. Translation: AAH47710.1.
IPI	IPI00419497.
RefSeq	NP_001154924.1. NM_001161452.1. NP_705839.3. NM_153611.4.
UniGene	Hs.22546.
3D structure databases
ProteinModelPortal	Q8NBI2.
ModBase	Search...
Polymorphism databases
DMDM	74730147.
Proteomic databases
PRIDE	Q8NBI2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000294072; ENSP00000294072; ENSG00000162144. ENST00000536915; ENSP00000437390; ENSG00000162144.
GeneID	220002.
KEGG	hsa:220002.
UCSC	uc001nrf.4. human.
Organism-specific databases
CTD	220002.
GeneCards	GC11M061116.
HGNC	HGNC:23014. CYBASC3.
HPA	HPA052548.
neXtProt	NX_Q8NBI2.
PharmGKB	PA134880796.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG327154.
HOGENOM	HOG000231043.
HOVERGEN	HBG054164.
InParanoid	Q8NBI2.
KO	K16295.
OrthoDB	EOG4QZ7MV.
PhylomeDB	Q8NBI2.
Gene expression databases
ArrayExpress	Q8NBI2.
Bgee	Q8NBI2.
CleanEx	HS_CYBASC3.
Genevestigator	Q8NBI2.
Family and domain databases
InterPro	IPR006593. Cyt_b561/ferric_Rdtase_TM. IPR004877. Cyt_b561_euk. [Graphical view]
Pfam	PF03188. Cytochrom_B561. 1 hit. [Graphical view]
SMART	SM00665. B561. 1 hit. [Graphical view]
PROSITE	PS50939. CYTOCHROME_B561. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CYBASC3. human.
GenomeRNAi	220002.
NextBio	90945.

Entry information

Entry name

CYAC3_HUMAN

Accession

Primary (citable) accession number: Q8NBI2
Secondary accession number(s): B3KPU2, Q6PK96

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.