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Protein

Cytochrome b ascorbate-dependent protein 3

Gene

CYB561A3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Ferric-chelate reductase that reduces Fe3+ to Fe2+ before its transport from the endosome to the cytoplasm. Probably uses ascorbate as electron donor (By similarity).By similarity

Cofactori

hemeBy similarityNote: Binds 2 heme groups non-covalently.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi83Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi87Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi105Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi117Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi156Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000162144-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b ascorbate-dependent protein 3 (EC:1.-.-.-)
Alternative name(s):
Cytochrome b561 family member A3
Lysosomal cytochrome b
Short name:
LCytb
Gene namesi
Name:CYB561A3
Synonyms:CYBASC3, LCYTB
ORF Names:PSEC0259
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:23014. CYB561A3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei8 – 28Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei46 – 66Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei84 – 104Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei120 – 140Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei155 – 175Helical; Name=5Sequence analysisAdd BLAST21
Transmembranei203 – 223Helical; Name=6Sequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • late endosome membrane Source: UniProtKB
  • lysosomal membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000162144.
PharmGKBiPA134880796.

Polymorphism and mutation databases

BioMutaiCYB561A3.
DMDMi74730147.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003148381 – 242Cytochrome b ascorbate-dependent protein 3Add BLAST242

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi38N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8NBI2.
PaxDbiQ8NBI2.
PeptideAtlasiQ8NBI2.
PRIDEiQ8NBI2.

Expressioni

Gene expression databases

BgeeiENSG00000162144.
CleanExiHS_CYBASC3.
ExpressionAtlasiQ8NBI2. baseline and differential.
GenevisibleiQ8NBI2. HS.

Organism-specific databases

HPAiHPA052548.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RNF5Q999425EBI-10269179,EBI-348482

Protein-protein interaction databases

BioGridi128617. 2 interactors.
IntActiQ8NBI2. 3 interactors.
STRINGi9606.ENSP00000398979.

Structurei

3D structure databases

ProteinModelPortaliQ8NBI2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 219Cytochrome b561PROSITE-ProRule annotationAdd BLAST208

Sequence similaritiesi

Contains 1 cytochrome b561 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1619. Eukaryota.
ENOG4111FKI. LUCA.
GeneTreeiENSGT00390000010986.
HOGENOMiHOG000231043.
HOVERGENiHBG054164.
InParanoidiQ8NBI2.
KOiK16295.
PhylomeDBiQ8NBI2.
TreeFamiTF314222.

Family and domain databases

InterProiIPR028836. Cyb561a3.
IPR006593. Cyt_b561/ferric_Rdtase_TM.
[Graphical view]
PANTHERiPTHR10106:SF0. PTHR10106:SF0. 1 hit.
PfamiPF03188. Cytochrom_B561. 1 hit.
[Graphical view]
SMARTiSM00665. B561. 1 hit.
[Graphical view]
PROSITEiPS50939. CYTOCHROME_B561. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8NBI2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSGRFYLSC LLLGSLGSMC ILFTIYWMQY WRGGFAWNGS IYMFNWHPVL
60 70 80 90 100
MVAGMVVFYG GASLVYRLPQ SWVGPKLPWK LLHAALHLMA FVLTVVGLVA
110 120 130 140 150
VFTFHNHGRT ANLYSLHSWL GITTVFLFAC QWFLGFAVFL LPWASMWLRS
160 170 180 190 200
LLKPIHVFFG AAILSLSIAS VISGINEKLF FSLKNTTRPY HSLPSEAVFA
210 220 230 240
NSTGMLVVAF GLLVLYILLA SSWKRPEPGI LTDRQPLLHD GE
Length:242
Mass (Da):27,214
Last modified:October 1, 2002 - v1
Checksum:i1D0C4904CEAED747
GO
Isoform 2 (identifier: Q8NBI2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGKNFSDSFLSRECVIRM

Show »
Length:259
Mass (Da):29,186
Checksum:i426A35886A289F17
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti122I → T in AAH04391 (PubMed:15489334).Curated1
Sequence conflicti168I → N in BAG59601 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0473581M → MGKNFSDSFLSRECVIRM in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056751 mRNA. Translation: BAG51804.1.
AK075559 mRNA. Translation: BAC11698.1.
AK297084 mRNA. Translation: BAG59601.1.
AP003108 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73941.1.
CH471076 Genomic DNA. Translation: EAW73942.1.
BC004391 mRNA. Translation: AAH04391.1.
BC014045 mRNA. Translation: AAH14045.2.
BC047710 mRNA. Translation: AAH47710.1.
CCDSiCCDS53639.1. [Q8NBI2-2]
CCDS8004.1. [Q8NBI2-1]
RefSeqiNP_001154924.1. NM_001161452.1. [Q8NBI2-1]
NP_001154926.1. NM_001161454.1. [Q8NBI2-2]
NP_001287692.1. NM_001300763.1.
NP_705839.3. NM_153611.4. [Q8NBI2-1]
UniGeneiHs.22546.

Genome annotation databases

EnsembliENST00000294072; ENSP00000294072; ENSG00000162144. [Q8NBI2-1]
ENST00000426130; ENSP00000398979; ENSG00000162144. [Q8NBI2-2]
ENST00000536915; ENSP00000437390; ENSG00000162144. [Q8NBI2-1]
GeneIDi220002.
KEGGihsa:220002.
UCSCiuc001nrg.4. human. [Q8NBI2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056751 mRNA. Translation: BAG51804.1.
AK075559 mRNA. Translation: BAC11698.1.
AK297084 mRNA. Translation: BAG59601.1.
AP003108 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW73941.1.
CH471076 Genomic DNA. Translation: EAW73942.1.
BC004391 mRNA. Translation: AAH04391.1.
BC014045 mRNA. Translation: AAH14045.2.
BC047710 mRNA. Translation: AAH47710.1.
CCDSiCCDS53639.1. [Q8NBI2-2]
CCDS8004.1. [Q8NBI2-1]
RefSeqiNP_001154924.1. NM_001161452.1. [Q8NBI2-1]
NP_001154926.1. NM_001161454.1. [Q8NBI2-2]
NP_001287692.1. NM_001300763.1.
NP_705839.3. NM_153611.4. [Q8NBI2-1]
UniGeneiHs.22546.

3D structure databases

ProteinModelPortaliQ8NBI2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128617. 2 interactors.
IntActiQ8NBI2. 3 interactors.
STRINGi9606.ENSP00000398979.

Polymorphism and mutation databases

BioMutaiCYB561A3.
DMDMi74730147.

Proteomic databases

MaxQBiQ8NBI2.
PaxDbiQ8NBI2.
PeptideAtlasiQ8NBI2.
PRIDEiQ8NBI2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000294072; ENSP00000294072; ENSG00000162144. [Q8NBI2-1]
ENST00000426130; ENSP00000398979; ENSG00000162144. [Q8NBI2-2]
ENST00000536915; ENSP00000437390; ENSG00000162144. [Q8NBI2-1]
GeneIDi220002.
KEGGihsa:220002.
UCSCiuc001nrg.4. human. [Q8NBI2-1]

Organism-specific databases

CTDi220002.
GeneCardsiCYB561A3.
HGNCiHGNC:23014. CYB561A3.
HPAiHPA052548.
neXtProtiNX_Q8NBI2.
OpenTargetsiENSG00000162144.
PharmGKBiPA134880796.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1619. Eukaryota.
ENOG4111FKI. LUCA.
GeneTreeiENSGT00390000010986.
HOGENOMiHOG000231043.
HOVERGENiHBG054164.
InParanoidiQ8NBI2.
KOiK16295.
PhylomeDBiQ8NBI2.
TreeFamiTF314222.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000162144-MONOMER.

Miscellaneous databases

ChiTaRSiCYB561A3. human.
GenomeRNAii220002.
PROiQ8NBI2.

Gene expression databases

BgeeiENSG00000162144.
CleanExiHS_CYBASC3.
ExpressionAtlasiQ8NBI2. baseline and differential.
GenevisibleiQ8NBI2. HS.

Family and domain databases

InterProiIPR028836. Cyb561a3.
IPR006593. Cyt_b561/ferric_Rdtase_TM.
[Graphical view]
PANTHERiPTHR10106:SF0. PTHR10106:SF0. 1 hit.
PfamiPF03188. Cytochrom_B561. 1 hit.
[Graphical view]
SMARTiSM00665. B561. 1 hit.
[Graphical view]
PROSITEiPS50939. CYTOCHROME_B561. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYAC3_HUMAN
AccessioniPrimary (citable) accession number: Q8NBI2
Secondary accession number(s): B3KPU2
, B4DLN9, J3KQH4, Q6PK96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.