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Q8NB90

- SPAT5_HUMAN

UniProt

Q8NB90 - SPAT5_HUMAN

Protein

Spermatogenesis-associated protein 5

Gene

SPATA5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 3 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    May be involved in morphological and functional mitochondrial transformations during spermatogenesis.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi394 – 4018ATP 1Sequence Analysis
    Nucleotide bindingi668 – 6758ATP 2Sequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. nucleoside-triphosphatase activity Source: InterPro

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. multicellular organismal development Source: UniProtKB-KW
    3. spermatogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Spermatogenesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Spermatogenesis-associated protein 5
    Alternative name(s):
    ATPase family protein 2 homolog
    Spermatogenesis-associated factor protein
    Gene namesi
    Name:SPATA5
    Synonyms:AFG2, SPAF
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:18119. SPATA5.

    Subcellular locationi

    Cytoplasm By similarity. Mitochondrion By similarity

    GO - Cellular componenti

    1. cytoplasm Source: LIFEdb
    2. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA38294.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 893893Spermatogenesis-associated protein 5PRO_0000330583Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei272 – 2721Phosphothreonine1 Publication
    Modified residuei274 – 2741Phosphoserine1 Publication
    Modified residuei279 – 2791Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8NB90.
    PaxDbiQ8NB90.
    PRIDEiQ8NB90.

    PTM databases

    PhosphoSiteiQ8NB90.

    Expressioni

    Gene expression databases

    BgeeiQ8NB90.
    CleanExiHS_SPATA5.
    GenevestigatoriQ8NB90.

    Organism-specific databases

    HPAiHPA036450.
    HPA036451.

    Interactioni

    Protein-protein interaction databases

    BioGridi127927. 1 interaction.
    IntActiQ8NB90. 3 interactions.
    MINTiMINT-3041496.
    STRINGi9606.ENSP00000274008.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8NB90.
    SMRiQ8NB90. Positions 339-889.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family. AFG2 subfamily.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0464.
    HOGENOMiHOG000223225.
    HOVERGENiHBG108504.
    InParanoidiQ8NB90.
    KOiK14575.
    OMAiMNDIRPS.
    OrthoDBiEOG7HF1HK.
    PhylomeDBiQ8NB90.
    TreeFamiTF314525.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 2 hits.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    SM01073. CDC48_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    PROSITEiPS00674. AAA. 2 hits.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8NB90-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSKKNRKRL NQSAENGSSL PSAASSCAEA RAPSAGSDFA ATSGTLTVTN    50
    LLEKVDDKIP KTFQNSLIHL GLNTMKSANI CIGRPVLLTS LNGKQEVYTA 100
    WPMAGFPGGK VGLSEMAQKN VGVRPGDAIQ VQPLVGAVLQ AEEMDVALSD 150
    KDMEINEEEL TGCILRKLDG KIVLPGNFLY CTFYGRPYKL QVLRVKGADG 200
    MILGGPQSDS DTDAQRMAFE QSSMETSSLE LSLQLSQLDL EDTQIPTSRS 250
    TPYKPIDDRI TNKASDVLLD VTQSPGDGSG LMLEEVTGLK CNFESAREGN 300
    EQLTEEERLL KFSIGAKCNT DTFYFISSTT RVNFTEIDKN SKEQDNQFKV 350
    TYDMIGGLSS QLKAIREIIE LPLKQPELFK SYGIPAPRGV LLYGPPGTGK 400
    TMIARAVANE VGAYVSVING PEIISKFYGE TEAKLRQIFA EATLRHPSII 450
    FIDELDALCP KREGAQNEVE KRVVASLLTL MDGIGSEVSE GQVLVLGATN 500
    RPHALDAALR RPGRFDKEIE IGVPNAQDRL DILQKLLRRV PHLLTEAELL 550
    QLANSAHGYV GADLKVLCNE AGLCALRRIL KKQPNLPDVK VAGLVKITLK 600
    DFLQAMNDIR PSAMREIAID VPNVSWSDIG GLESIKLKLE QAVEWPLKHP 650
    ESFIRMGIQP PKGVLLYGPP GCSKTMIAKA LANESGLNFL AIKGPELMNK 700
    YVGESERAVR ETFRKARAVA PSIIFFDELD ALAVERGSSL GAGNVADRVL 750
    AQLLTEMDGI EQLKDVTILA ATNRPDRIDK ALMRPGRIDR IIYVPLPDAA 800
    TRREIFKLQF HSMPVSNEVD LDELILQTDA YSGAEIVAVC REAALLALEE 850
    DIQANLIMKR HFTQALSTVT PRIPESLRRF YEDYQEKSGL HTL 893
    Length:893
    Mass (Da):97,904
    Last modified:October 5, 2010 - v3
    Checksum:i60214147CBB31F4A
    GO
    Isoform 2 (identifier: Q8NB90-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         781-790: ALMRPGRIDR → VPPSQTFLLL
         791-893: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:790
    Mass (Da):86,077
    Checksum:iFFFEED99D59D405F
    GO
    Isoform 3 (identifier: Q8NB90-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         694-696: GPE → VGC
         697-893: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:696
    Mass (Da):75,727
    Checksum:i5403422972A776F5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti55 – 551Missing in AAH48217. (PubMed:15489334)Curated
    Sequence conflicti66 – 661S → P in AAM00262. 1 PublicationCurated
    Sequence conflicti210 – 2101S → P in AAM00262. 1 PublicationCurated
    Sequence conflicti568 – 5681C → Y in BAC03651. (PubMed:14702039)Curated
    Sequence conflicti603 – 6031L → F in AAM00262. 1 PublicationCurated
    Sequence conflicti614 – 6141M → T in BAC03651. (PubMed:14702039)Curated
    Sequence conflicti695 – 6951P → S in AAM43608. 1 PublicationCurated
    Sequence conflicti851 – 8511D → G in AAM00262. 1 PublicationCurated
    Sequence conflicti891 – 8911H → R in AAM00262. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti27 – 271C → S.
    Corresponds to variant rs35430470 [ dbSNP | Ensembl ].
    VAR_042703
    Natural varianti673 – 6731S → Y.
    Corresponds to variant rs35133326 [ dbSNP | Ensembl ].
    VAR_042704

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei694 – 6963GPE → VGC in isoform 3. 1 PublicationVSP_033046
    Alternative sequencei697 – 893197Missing in isoform 3. 1 PublicationVSP_033047Add
    BLAST
    Alternative sequencei781 – 79010ALMRPGRIDR → VPPSQTFLLL in isoform 2. 1 PublicationVSP_033048
    Alternative sequencei791 – 893103Missing in isoform 2. 1 PublicationVSP_033049Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF361489 mRNA. Translation: AAM00262.1.
    AF479656 mRNA. Translation: AAM43608.1.
    AK091384 mRNA. Translation: BAC03651.1.
    AC021205 Genomic DNA. No translation available.
    AC026402 Genomic DNA. No translation available.
    AC097492 Genomic DNA. No translation available.
    AC109357 Genomic DNA. No translation available.
    BC048217 mRNA. Translation: AAH48217.1.
    CCDSiCCDS3730.1. [Q8NB90-1]
    RefSeqiNP_660208.2. NM_145207.2. [Q8NB90-1]
    UniGeneiHs.709648.

    Genome annotation databases

    EnsembliENST00000274008; ENSP00000274008; ENSG00000145375. [Q8NB90-1]
    GeneIDi166378.
    KEGGihsa:166378.
    UCSCiuc003iey.3. human. [Q8NB90-3]
    uc003iez.4. human. [Q8NB90-1]

    Polymorphism databases

    DMDMi308153554.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF361489 mRNA. Translation: AAM00262.1 .
    AF479656 mRNA. Translation: AAM43608.1 .
    AK091384 mRNA. Translation: BAC03651.1 .
    AC021205 Genomic DNA. No translation available.
    AC026402 Genomic DNA. No translation available.
    AC097492 Genomic DNA. No translation available.
    AC109357 Genomic DNA. No translation available.
    BC048217 mRNA. Translation: AAH48217.1 .
    CCDSi CCDS3730.1. [Q8NB90-1 ]
    RefSeqi NP_660208.2. NM_145207.2. [Q8NB90-1 ]
    UniGenei Hs.709648.

    3D structure databases

    ProteinModelPortali Q8NB90.
    SMRi Q8NB90. Positions 339-889.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 127927. 1 interaction.
    IntActi Q8NB90. 3 interactions.
    MINTi MINT-3041496.
    STRINGi 9606.ENSP00000274008.

    Chemistry

    ChEMBLi CHEMBL2311230.

    PTM databases

    PhosphoSitei Q8NB90.

    Polymorphism databases

    DMDMi 308153554.

    Proteomic databases

    MaxQBi Q8NB90.
    PaxDbi Q8NB90.
    PRIDEi Q8NB90.

    Protocols and materials databases

    DNASUi 166378.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000274008 ; ENSP00000274008 ; ENSG00000145375 . [Q8NB90-1 ]
    GeneIDi 166378.
    KEGGi hsa:166378.
    UCSCi uc003iey.3. human. [Q8NB90-3 ]
    uc003iez.4. human. [Q8NB90-1 ]

    Organism-specific databases

    CTDi 166378.
    GeneCardsi GC04P123844.
    H-InvDB HIX0022056.
    HGNCi HGNC:18119. SPATA5.
    HPAi HPA036450.
    HPA036451.
    MIMi 613940. gene.
    neXtProti NX_Q8NB90.
    PharmGKBi PA38294.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0464.
    HOGENOMi HOG000223225.
    HOVERGENi HBG108504.
    InParanoidi Q8NB90.
    KOi K14575.
    OMAi MNDIRPS.
    OrthoDBi EOG7HF1HK.
    PhylomeDBi Q8NB90.
    TreeFami TF314525.

    Miscellaneous databases

    GeneWikii SPATA5.
    GenomeRNAii 166378.
    NextBioi 88589.
    PROi Q8NB90.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8NB90.
    CleanExi HS_SPATA5.
    Genevestigatori Q8NB90.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 2 hits.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    SM01073. CDC48_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    PROSITEi PS00674. AAA. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of human SPAF, a new member of AAA-protein family associated with spermatogenesis and malignant conversion."
      Wang Z., Liu Y., Kulesz-Martin M.F.
      Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Cloning and characterization of a novel hSPAF gene."
      Xu J., Wang L., Ye X., Zeng L., Xie Y., Mao Y.
      Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Testis.
    6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-272; SER-274 AND SER-279, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSPAT5_HUMAN
    AccessioniPrimary (citable) accession number: Q8NB90
    Secondary accession number(s): C9JT97
    , Q86XW1, Q8NI20, Q8TDL7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 108 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3