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Q8NB90 (SPAT5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Spermatogenesis-associated protein 5
Alternative name(s):
ATPase family protein 2 homolog
Spermatogenesis-associated factor protein
Gene names
Name:SPATA5
Synonyms:AFG2, SPAF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length893 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in morphological and functional mitochondrial transformations during spermatogenesis By similarity.

Subcellular location

Cytoplasm By similarity. Mitochondrion By similarity.

Sequence similarities

Belongs to the AAA ATPase family. AFG2 subfamily.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8NB90-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8NB90-2)

The sequence of this isoform differs from the canonical sequence as follows:
     781-790: ALMRPGRIDR → VPPSQTFLLL
     791-893: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8NB90-3)

The sequence of this isoform differs from the canonical sequence as follows:
     694-696: GPE → VGC
     697-893: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 893893Spermatogenesis-associated protein 5
PRO_0000330583

Regions

Nucleotide binding394 – 4018ATP 1 Potential
Nucleotide binding668 – 6758ATP 2 Potential

Amino acid modifications

Modified residue2721Phosphothreonine Ref.6
Modified residue2741Phosphoserine Ref.6
Modified residue2791Phosphoserine Ref.6

Natural variations

Alternative sequence694 – 6963GPE → VGC in isoform 3.
VSP_033046
Alternative sequence697 – 893197Missing in isoform 3.
VSP_033047
Alternative sequence781 – 79010ALMRPGRIDR → VPPSQTFLLL in isoform 2.
VSP_033048
Alternative sequence791 – 893103Missing in isoform 2.
VSP_033049
Natural variant271C → S.
Corresponds to variant rs35430470 [ dbSNP | Ensembl ].
VAR_042703
Natural variant6731S → Y.
Corresponds to variant rs35133326 [ dbSNP | Ensembl ].
VAR_042704

Experimental info

Sequence conflict551Missing in AAH48217. Ref.5
Sequence conflict661S → P in AAM00262. Ref.1
Sequence conflict2101S → P in AAM00262. Ref.1
Sequence conflict5681C → Y in BAC03651. Ref.3
Sequence conflict6031L → F in AAM00262. Ref.1
Sequence conflict6141M → T in BAC03651. Ref.3
Sequence conflict6951P → S in AAM43608. Ref.2
Sequence conflict8511D → G in AAM00262. Ref.1
Sequence conflict8911H → R in AAM00262. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 5, 2010. Version 3.
Checksum: 60214147CBB31F4A

FASTA89397,904
        10         20         30         40         50         60 
MSSKKNRKRL NQSAENGSSL PSAASSCAEA RAPSAGSDFA ATSGTLTVTN LLEKVDDKIP 

        70         80         90        100        110        120 
KTFQNSLIHL GLNTMKSANI CIGRPVLLTS LNGKQEVYTA WPMAGFPGGK VGLSEMAQKN 

       130        140        150        160        170        180 
VGVRPGDAIQ VQPLVGAVLQ AEEMDVALSD KDMEINEEEL TGCILRKLDG KIVLPGNFLY 

       190        200        210        220        230        240 
CTFYGRPYKL QVLRVKGADG MILGGPQSDS DTDAQRMAFE QSSMETSSLE LSLQLSQLDL 

       250        260        270        280        290        300 
EDTQIPTSRS TPYKPIDDRI TNKASDVLLD VTQSPGDGSG LMLEEVTGLK CNFESAREGN 

       310        320        330        340        350        360 
EQLTEEERLL KFSIGAKCNT DTFYFISSTT RVNFTEIDKN SKEQDNQFKV TYDMIGGLSS 

       370        380        390        400        410        420 
QLKAIREIIE LPLKQPELFK SYGIPAPRGV LLYGPPGTGK TMIARAVANE VGAYVSVING 

       430        440        450        460        470        480 
PEIISKFYGE TEAKLRQIFA EATLRHPSII FIDELDALCP KREGAQNEVE KRVVASLLTL 

       490        500        510        520        530        540 
MDGIGSEVSE GQVLVLGATN RPHALDAALR RPGRFDKEIE IGVPNAQDRL DILQKLLRRV 

       550        560        570        580        590        600 
PHLLTEAELL QLANSAHGYV GADLKVLCNE AGLCALRRIL KKQPNLPDVK VAGLVKITLK 

       610        620        630        640        650        660 
DFLQAMNDIR PSAMREIAID VPNVSWSDIG GLESIKLKLE QAVEWPLKHP ESFIRMGIQP 

       670        680        690        700        710        720 
PKGVLLYGPP GCSKTMIAKA LANESGLNFL AIKGPELMNK YVGESERAVR ETFRKARAVA 

       730        740        750        760        770        780 
PSIIFFDELD ALAVERGSSL GAGNVADRVL AQLLTEMDGI EQLKDVTILA ATNRPDRIDK 

       790        800        810        820        830        840 
ALMRPGRIDR IIYVPLPDAA TRREIFKLQF HSMPVSNEVD LDELILQTDA YSGAEIVAVC 

       850        860        870        880        890 
REAALLALEE DIQANLIMKR HFTQALSTVT PRIPESLRRF YEDYQEKSGL HTL 

« Hide

Isoform 2 [UniParc].

Checksum: FFFEED99D59D405F
Show »

FASTA79086,077
Isoform 3 [UniParc].

Checksum: 5403422972A776F5
Show »

FASTA69675,727

References

« Hide 'large scale' references
[1]"Cloning and characterization of human SPAF, a new member of AAA-protein family associated with spermatogenesis and malignant conversion."
Wang Z., Liu Y., Kulesz-Martin M.F.
Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning and characterization of a novel hSPAF gene."
Xu J., Wang L., Ye X., Zeng L., Xie Y., Mao Y.
Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Testis.
[6]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-272; SER-274 AND SER-279, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF361489 mRNA. Translation: AAM00262.1.
AF479656 mRNA. Translation: AAM43608.1.
AK091384 mRNA. Translation: BAC03651.1.
AC021205 Genomic DNA. No translation available.
AC026402 Genomic DNA. No translation available.
AC097492 Genomic DNA. No translation available.
AC109357 Genomic DNA. No translation available.
BC048217 mRNA. Translation: AAH48217.1.
RefSeqNP_660208.2. NM_145207.2.
UniGeneHs.709648.

3D structure databases

ProteinModelPortalQ8NB90.
SMRQ8NB90. Positions 42-888.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127927. 1 interaction.
IntActQ8NB90. 3 interactions.
MINTMINT-3041496.
STRING9606.ENSP00000274008.

Chemistry

ChEMBLCHEMBL2311230.

PTM databases

PhosphoSiteQ8NB90.

Polymorphism databases

DMDM308153554.

Proteomic databases

PaxDbQ8NB90.
PRIDEQ8NB90.

Protocols and materials databases

DNASU166378.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000274008; ENSP00000274008; ENSG00000145375. [Q8NB90-1]
GeneID166378.
KEGGhsa:166378.
UCSCuc003iey.3. human. [Q8NB90-3]
uc003iez.4. human. [Q8NB90-1]

Organism-specific databases

CTD166378.
GeneCardsGC04P123844.
H-InvDBHIX0022056.
HGNCHGNC:18119. SPATA5.
HPAHPA036450.
HPA036451.
MIM613940. gene.
neXtProtNX_Q8NB90.
PharmGKBPA38294.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0464.
HOGENOMHOG000223225.
HOVERGENHBG108504.
InParanoidQ8NB90.
KOK14575.
OMAMNDIRPS.
OrthoDBEOG7HF1HK.
PhylomeDBQ8NB90.
TreeFamTF314525.

Gene expression databases

BgeeQ8NB90.
CleanExHS_SPATA5.
GenevestigatorQ8NB90.

Family and domain databases

InterProIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00004. AAA. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEPS00674. AAA. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSPATA5.
GenomeRNAi166378.
NextBio88589.
PROQ8NB90.
SOURCESearch...

Entry information

Entry nameSPAT5_HUMAN
AccessionPrimary (citable) accession number: Q8NB90
Secondary accession number(s): C9JT97 expand/collapse secondary AC list , Q86XW1, Q8NI20, Q8TDL7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 5, 2010
Last modified: March 19, 2014
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM