Q8NB90 (SPAT5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermatogenesis-associated protein 5

Alternative name(s):
ATPase family protein 2 homolog
Spermatogenesis-associated factor protein

Gene names

Name:	SPATA5
Synonyms:	AFG2, SPAF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	893 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in morphological and functional mitochondrial transformations during spermatogenesis By similarity.
Subcellular location	Cytoplasm By similarity. Mitochondrion By similarity.
Sequence similarities	Belongs to the AAA ATPase family. AFG2 subfamily.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis
Cellular component	Cytoplasm Mitochondrion
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay. Source: LIFEdb mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8NB90-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8NB90-2)

The sequence of this isoform differs from the canonical sequence as follows:
     781-790: ALMRPGRIDR → VPPSQTFLLL
     791-893: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8NB90-3)

The sequence of this isoform differs from the canonical sequence as follows:
     694-696: GPE → VGC
     697-893: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 893	893	Spermatogenesis-associated protein 5		PRO_0000330583
Regions
	Nucleotide binding	394 – 401	8	ATP 1 Potential
	Nucleotide binding	668 – 675	8	ATP 2 Potential
Amino acid modifications
	Modified residue	272	1	Phosphothreonine Ref.6
	Modified residue	274	1	Phosphoserine Ref.6
	Modified residue	279	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	694 – 696	3	GPE → VGC in isoform 3.		VSP_033046
	Alternative sequence	697 – 893	197	Missing in isoform 3.		VSP_033047
	Alternative sequence	781 – 790	10	ALMRPGRIDR → VPPSQTFLLL in isoform 2.		VSP_033048
	Alternative sequence	791 – 893	103	Missing in isoform 2.		VSP_033049
	Natural variant	27	1	C → S. Corresponds to variant rs35430470 [ dbSNP | Ensembl ].		VAR_042703
	Natural variant	673	1	S → Y. Corresponds to variant rs35133326 [ dbSNP | Ensembl ].		VAR_042704
Experimental info
	Sequence conflict	55	1	Missing in AAH48217. Ref.5
	Sequence conflict	66	1	S → P in AAM00262. Ref.1
	Sequence conflict	210	1	S → P in AAM00262. Ref.1
	Sequence conflict	568	1	C → Y in BAC03651. Ref.3
	Sequence conflict	603	1	L → F in AAM00262. Ref.1
	Sequence conflict	614	1	M → T in BAC03651. Ref.3
	Sequence conflict	695	1	P → S in AAM43608. Ref.2
	Sequence conflict	851	1	D → G in AAM00262. Ref.1
	Sequence conflict	891	1	H → R in AAM00262. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 5, 2010. Version 3. Checksum: 60214147CBB31F4A	FASTA	893	97,904
	10 20 30 40 50 60 MSSKKNRKRL NQSAENGSSL PSAASSCAEA RAPSAGSDFA ATSGTLTVTN LLEKVDDKIP 70 80 90 100 110 120 KTFQNSLIHL GLNTMKSANI CIGRPVLLTS LNGKQEVYTA WPMAGFPGGK VGLSEMAQKN 130 140 150 160 170 180 VGVRPGDAIQ VQPLVGAVLQ AEEMDVALSD KDMEINEEEL TGCILRKLDG KIVLPGNFLY 190 200 210 220 230 240 CTFYGRPYKL QVLRVKGADG MILGGPQSDS DTDAQRMAFE QSSMETSSLE LSLQLSQLDL 250 260 270 280 290 300 EDTQIPTSRS TPYKPIDDRI TNKASDVLLD VTQSPGDGSG LMLEEVTGLK CNFESAREGN 310 320 330 340 350 360 EQLTEEERLL KFSIGAKCNT DTFYFISSTT RVNFTEIDKN SKEQDNQFKV TYDMIGGLSS 370 380 390 400 410 420 QLKAIREIIE LPLKQPELFK SYGIPAPRGV LLYGPPGTGK TMIARAVANE VGAYVSVING 430 440 450 460 470 480 PEIISKFYGE TEAKLRQIFA EATLRHPSII FIDELDALCP KREGAQNEVE KRVVASLLTL 490 500 510 520 530 540 MDGIGSEVSE GQVLVLGATN RPHALDAALR RPGRFDKEIE IGVPNAQDRL DILQKLLRRV 550 560 570 580 590 600 PHLLTEAELL QLANSAHGYV GADLKVLCNE AGLCALRRIL KKQPNLPDVK VAGLVKITLK 610 620 630 640 650 660 DFLQAMNDIR PSAMREIAID VPNVSWSDIG GLESIKLKLE QAVEWPLKHP ESFIRMGIQP 670 680 690 700 710 720 PKGVLLYGPP GCSKTMIAKA LANESGLNFL AIKGPELMNK YVGESERAVR ETFRKARAVA 730 740 750 760 770 780 PSIIFFDELD ALAVERGSSL GAGNVADRVL AQLLTEMDGI EQLKDVTILA ATNRPDRIDK 790 800 810 820 830 840 ALMRPGRIDR IIYVPLPDAA TRREIFKLQF HSMPVSNEVD LDELILQTDA YSGAEIVAVC 850 860 870 880 890 REAALLALEE DIQANLIMKR HFTQALSTVT PRIPESLRRF YEDYQEKSGL HTL
	Isoform 2 [UniParc]. Checksum: FFFEED99D59D405F	FASTA	790	86,077
	10 20 30 40 50 60 MSSKKNRKRL NQSAENGSSL PSAASSCAEA RAPSAGSDFA ATSGTLTVTN LLEKVDDKIP 70 80 90 100 110 120 KTFQNSLIHL GLNTMKSANI CIGRPVLLTS LNGKQEVYTA WPMAGFPGGK VGLSEMAQKN 130 140 150 160 170 180 VGVRPGDAIQ VQPLVGAVLQ AEEMDVALSD KDMEINEEEL TGCILRKLDG KIVLPGNFLY 190 200 210 220 230 240 CTFYGRPYKL QVLRVKGADG MILGGPQSDS DTDAQRMAFE QSSMETSSLE LSLQLSQLDL 250 260 270 280 290 300 EDTQIPTSRS TPYKPIDDRI TNKASDVLLD VTQSPGDGSG LMLEEVTGLK CNFESAREGN 310 320 330 340 350 360 EQLTEEERLL KFSIGAKCNT DTFYFISSTT RVNFTEIDKN SKEQDNQFKV TYDMIGGLSS 370 380 390 400 410 420 QLKAIREIIE LPLKQPELFK SYGIPAPRGV LLYGPPGTGK TMIARAVANE VGAYVSVING 430 440 450 460 470 480 PEIISKFYGE TEAKLRQIFA EATLRHPSII FIDELDALCP KREGAQNEVE KRVVASLLTL 490 500 510 520 530 540 MDGIGSEVSE GQVLVLGATN RPHALDAALR RPGRFDKEIE IGVPNAQDRL DILQKLLRRV 550 560 570 580 590 600 PHLLTEAELL QLANSAHGYV GADLKVLCNE AGLCALRRIL KKQPNLPDVK VAGLVKITLK 610 620 630 640 650 660 DFLQAMNDIR PSAMREIAID VPNVSWSDIG GLESIKLKLE QAVEWPLKHP ESFIRMGIQP 670 680 690 700 710 720 PKGVLLYGPP GCSKTMIAKA LANESGLNFL AIKGPELMNK YVGESERAVR ETFRKARAVA 730 740 750 760 770 780 PSIIFFDELD ALAVERGSSL GAGNVADRVL AQLLTEMDGI EQLKDVTILA ATNRPDRIDK 790 VPPSQTFLLL
	Isoform 3 [UniParc]. Checksum: 5403422972A776F5	FASTA	696	75,727
	10 20 30 40 50 60 MSSKKNRKRL NQSAENGSSL PSAASSCAEA RAPSAGSDFA ATSGTLTVTN LLEKVDDKIP 70 80 90 100 110 120 KTFQNSLIHL GLNTMKSANI CIGRPVLLTS LNGKQEVYTA WPMAGFPGGK VGLSEMAQKN 130 140 150 160 170 180 VGVRPGDAIQ VQPLVGAVLQ AEEMDVALSD KDMEINEEEL TGCILRKLDG KIVLPGNFLY 190 200 210 220 230 240 CTFYGRPYKL QVLRVKGADG MILGGPQSDS DTDAQRMAFE QSSMETSSLE LSLQLSQLDL 250 260 270 280 290 300 EDTQIPTSRS TPYKPIDDRI TNKASDVLLD VTQSPGDGSG LMLEEVTGLK CNFESAREGN 310 320 330 340 350 360 EQLTEEERLL KFSIGAKCNT DTFYFISSTT RVNFTEIDKN SKEQDNQFKV TYDMIGGLSS 370 380 390 400 410 420 QLKAIREIIE LPLKQPELFK SYGIPAPRGV LLYGPPGTGK TMIARAVANE VGAYVSVING 430 440 450 460 470 480 PEIISKFYGE TEAKLRQIFA EATLRHPSII FIDELDALCP KREGAQNEVE KRVVASLLTL 490 500 510 520 530 540 MDGIGSEVSE GQVLVLGATN RPHALDAALR RPGRFDKEIE IGVPNAQDRL DILQKLLRRV 550 560 570 580 590 600 PHLLTEAELL QLANSAHGYV GADLKVLCNE AGLCALRRIL KKQPNLPDVK VAGLVKITLK 610 620 630 640 650 660 DFLQAMNDIR PSAMREIAID VPNVSWSDIG GLESIKLKLE QAVEWPLKHP ESFIRMGIQP 670 680 690 PKGVLLYGPP GCSKTMIAKA LANESGLNFL AIKVGC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of human SPAF, a new member of AAA-protein family associated with spermatogenesis and malignant conversion." Wang Z., Liu Y., Kulesz-Martin M.F. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Cloning and characterization of a novel hSPAF gene." Xu J., Wang L., Ye X., Zeng L., Xie Y., Mao Y. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[6]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-272; SER-274 AND SER-279, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF361489 mRNA. Translation: AAM00262.1. AF479656 mRNA. Translation: AAM43608.1. AK091384 mRNA. Translation: BAC03651.1. AC021205 Genomic DNA. No translation available. AC026402 Genomic DNA. No translation available. AC097492 Genomic DNA. No translation available. AC109357 Genomic DNA. No translation available. BC048217 mRNA. Translation: AAH48217.1.
IPI	IPI00329583. IPI00890715. IPI00890727.
RefSeq	NP_660208.2. NM_145207.2.
UniGene	Hs.709648.
3D structure databases
ProteinModelPortal	Q8NB90.
SMR	Q8NB90. Positions 339-886.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8NB90. 1 interaction.
MINT	MINT-3041496.
STRING	9606.ENSP00000274008.
PTM databases
PhosphoSite	Q8NB90.
Polymorphism databases
DMDM	308153554.
Proteomic databases
PaxDb	Q8NB90.
PRIDE	Q8NB90.
Protocols and materials databases
DNASU	166378.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000274008; ENSP00000274008; ENSG00000145375.
GeneID	166378.
KEGG	hsa:166378.
UCSC	uc003iey.3. human. uc003iez.4. human.
Organism-specific databases
CTD	166378.
GeneCards	GC04P123844.
H-InvDB	HIX0022056.
HGNC	HGNC:18119. SPATA5.
HPA	HPA036451.
MIM	613940. gene.
neXtProt	NX_Q8NB90.
PharmGKB	PA38294.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0464.
HOGENOM	HOG000223225.
HOVERGEN	HBG108504.
InParanoid	Q8NB90.
KO	K14575.
OMA	NDIRPSA.
OrthoDB	EOG43FGWF.
PhylomeDB	Q8NB90.
Gene expression databases
Bgee	Q8NB90.
CleanEx	HS_SPATA5.
Genevestigator	Q8NB90.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR009010. Asp_de-COase-like_dom. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR003338. CDC4_N-term_subdom. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF00004. AAA. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. SM01073. CDC48_N. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit. SSF52540. SSF52540. 2 hits.
PROSITE	PS00674. AAA. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	166378.
NextBio	88589.
SOURCE	Search...

Entry information

Entry name

SPAT5_HUMAN

Accession

Primary (citable) accession number: Q8NB90
Secondary accession number(s): C9JT97 , Q86XW1, Q8NI20, Q8TDL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	October 5, 2010
Last modified:	May 29, 2013
This is version 96 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.