ID   IQUB_HUMAN              Reviewed;         791 AA.
AC   Q8NA54; A4D0X0; Q49AL6; Q4G189; Q5BJD9; Q6NUH6; Q8N9Y2;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 2.
DT   27-MAR-2024, entry version 153.
DE   RecName: Full=IQ motif and ubiquitin-like domain-containing protein;
GN   Name=IQUB;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12690205; DOI=10.1126/science.1083423;
RA   Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA   Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA   Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA   Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA   Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA   Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA   Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA   Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA   Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA   Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA   Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA   Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA   Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA   Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA   Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA   Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA   Adams M.D., Tsui L.-C.;
RT   "Human chromosome 7: DNA sequence and biology.";
RL   Science 300:767-772(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP   MET-126.
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   INTERACTION WITH ZMYND10.
RX   PubMed=29601588; DOI=10.1371/journal.pgen.1007316;
RA   Cho K.J., Noh S.H., Han S.M., Choi W.I., Kim H.Y., Yu S., Lee J.S.,
RA   Rim J.H., Lee M.G., Hildebrandt F., Gee H.Y.;
RT   "ZMYND10 stabilizes intermediate chain proteins in the cytoplasmic pre-
RT   assembly of dynein arms.";
RL   PLoS Genet. 14:E1007316-E1007316(2018).
RN   [5]
RP   STRUCTURE BY NMR OF 121-227.
RG   RIKEN structural genomics initiative (RSGI);
RT   "Solution structure of the novel identified ubiquitin-like domain in the
RT   human hypothetical protein FLJ35834.";
RL   Submitted (JUN-2006) to the PDB data bank.
RN   [6]
RP   VARIANT [LARGE SCALE ANALYSIS] HIS-735.
RX   PubMed=16959974; DOI=10.1126/science.1133427;
RA   Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA   Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA   Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA   Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA   Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA   Velculescu V.E.;
RT   "The consensus coding sequences of human breast and colorectal cancers.";
RL   Science 314:268-274(2006).
RN   [7]
RP   VARIANT 314-TYR--HIS-791 DEL, FUNCTION, INTERACTION WITH RSPH3 AND
RP   CALMODULIN, AND INVOLVEMENT IN SPERMATOGENIC FAILURE WITH RADIAL SPOKE
RP   DEFECTS.
RX   PubMed=36355624; DOI=10.1093/humrep/deac244;
RA   Zhang Z., Zhou H., Deng X., Zhang R., Qu R., Mu J., Liu R., Zeng Y.,
RA   Chen B., Wang L., Sang Q., Bao S.;
RT   "IQUB deficiency causes male infertility by affecting the activity of p-
RT   ERK1/2/RSPH3.";
RL   Hum. Reprod. 37:deac244-deac244(2022).
CC   -!- FUNCTION: Adapter protein that anchors the radial spoke 1 (RS1) complex
CC       to the A microtubule of outer doublet microtubules in axonemes
CC       (PubMed:36355624). The triple radial spokes (RS1, RS2 and RS3) are
CC       required to modulate beating of the sperm flagellum (PubMed:36355624).
CC       May play a role in inhibiting signaling via MAPK1/ERK2 and MAPK3/ERK1
CC       (PubMed:36355624). Additionally, may play a role in the functioning of
CC       cilia (By similarity). Not required for the functioning of tracheal or
CC       ependymal cilia (By similarity). {ECO:0000250|UniProtKB:Q8CDK3,
CC       ECO:0000269|PubMed:36355624}.
CC   -!- SUBUNIT: Component of the axonemal radial spoke 1 (RS1) complex, at
CC       least composed of spoke head proteins RSPH1, RSPH3, RSPH9 and the
CC       cilia-specific component RSPH4A or sperm-specific component RSPH6A,
CC       spoke stalk proteins RSPH14, DNAJB13, DYDC1, ROPN1L and NME5, and the
CC       anchor protein IQUB (By similarity). Does not appear to be part of
CC       radial spoke complexes 2 or 3 (RS2 or RS3) (By similarity). Interacts
CC       with CALM1 (By similarity). Interacts with DNAJB13 (By similarity).
CC       Interacts with DYNLL2 (By similarity). Interacts with NME5 (By
CC       similarity). Interacts with RSPH3 (PubMed:36355624). Interacts with
CC       RSPH9 (By similarity). Interacts with ZMYND10 (PubMed:29601588).
CC       Interacts with calmodulin; the interaction occurs in conditions of low
CC       but not high calcium (PubMed:36355624). {ECO:0000250|UniProtKB:Q8CDK3,
CC       ECO:0000269|PubMed:29601588, ECO:0000269|PubMed:36355624}.
CC   -!- INTERACTION:
CC       Q8NA54; Q9H161: ALX4; NbExp=3; IntAct=EBI-10220600, EBI-11317841;
CC       Q8NA54; Q9NYG5-2: ANAPC11; NbExp=3; IntAct=EBI-10220600, EBI-12224467;
CC       Q8NA54; Q86SG2: ANKRD23; NbExp=3; IntAct=EBI-10220600, EBI-5661893;
CC       Q8NA54; Q9H2G9: BLZF1; NbExp=6; IntAct=EBI-10220600, EBI-2548012;
CC       Q8NA54; Q13137: CALCOCO2; NbExp=3; IntAct=EBI-10220600, EBI-739580;
CC       Q8NA54; Q8NHQ1: CEP70; NbExp=3; IntAct=EBI-10220600, EBI-739624;
CC       Q8NA54; Q8TAP6: CEP76; NbExp=3; IntAct=EBI-10220600, EBI-742887;
CC       Q8NA54; P28329-3: CHAT; NbExp=3; IntAct=EBI-10220600, EBI-25837549;
CC       Q8NA54; Q96A83-2: COL26A1; NbExp=3; IntAct=EBI-10220600, EBI-21553822;
CC       Q8NA54; A8MQ03: CYSRT1; NbExp=3; IntAct=EBI-10220600, EBI-3867333;
CC       Q8NA54; Q14689-4: DIP2A; NbExp=3; IntAct=EBI-10220600, EBI-12019962;
CC       Q8NA54; O75190-2: DNAJB6; NbExp=3; IntAct=EBI-10220600, EBI-12593112;
CC       Q8NA54; O14645: DNALI1; NbExp=3; IntAct=EBI-10220600, EBI-395638;
CC       Q8NA54; P50570-2: DNM2; NbExp=3; IntAct=EBI-10220600, EBI-10968534;
CC       Q8NA54; P63167: DYNLL1; NbExp=3; IntAct=EBI-10220600, EBI-349105;
CC       Q8NA54; O00167-2: EYA2; NbExp=3; IntAct=EBI-10220600, EBI-12807776;
CC       Q8NA54; P22607: FGFR3; NbExp=3; IntAct=EBI-10220600, EBI-348399;
CC       Q8NA54; Q8TB36: GDAP1; NbExp=3; IntAct=EBI-10220600, EBI-11110431;
CC       Q8NA54; P14136: GFAP; NbExp=3; IntAct=EBI-10220600, EBI-744302;
CC       Q8NA54; Q08379: GOLGA2; NbExp=3; IntAct=EBI-10220600, EBI-618309;
CC       Q8NA54; Q6PI77: GPRASP3; NbExp=3; IntAct=EBI-10220600, EBI-11519926;
CC       Q8NA54; P06396: GSN; NbExp=3; IntAct=EBI-10220600, EBI-351506;
CC       Q8NA54; P01112: HRAS; NbExp=3; IntAct=EBI-10220600, EBI-350145;
CC       Q8NA54; P04792: HSPB1; NbExp=3; IntAct=EBI-10220600, EBI-352682;
CC       Q8NA54; Q8IYA8: IHO1; NbExp=4; IntAct=EBI-10220600, EBI-8638439;
CC       Q8NA54; Q8WXH2: JPH3; NbExp=3; IntAct=EBI-10220600, EBI-1055254;
CC       Q8NA54; Q9BW62: KATNAL1; NbExp=3; IntAct=EBI-10220600, EBI-743591;
CC       Q8NA54; O60333-2: KIF1B; NbExp=3; IntAct=EBI-10220600, EBI-10975473;
CC       Q8NA54; Q9BVG8-5: KIFC3; NbExp=3; IntAct=EBI-10220600, EBI-14069005;
CC       Q8NA54; O14901: KLF11; NbExp=3; IntAct=EBI-10220600, EBI-948266;
CC       Q8NA54; Q6A162: KRT40; NbExp=3; IntAct=EBI-10220600, EBI-10171697;
CC       Q8NA54; O95678: KRT75; NbExp=3; IntAct=EBI-10220600, EBI-2949715;
CC       Q8NA54; Q01546: KRT76; NbExp=3; IntAct=EBI-10220600, EBI-2952745;
CC       Q8NA54; P60409: KRTAP10-7; NbExp=3; IntAct=EBI-10220600, EBI-10172290;
CC       Q8NA54; P60411: KRTAP10-9; NbExp=3; IntAct=EBI-10220600, EBI-10172052;
CC       Q8NA54; P59991: KRTAP12-2; NbExp=3; IntAct=EBI-10220600, EBI-10176379;
CC       Q8NA54; P60329: KRTAP12-4; NbExp=6; IntAct=EBI-10220600, EBI-10176396;
CC       Q8NA54; Q9UBR4-2: LHX3; NbExp=3; IntAct=EBI-10220600, EBI-12039345;
CC       Q8NA54; P02545: LMNA; NbExp=3; IntAct=EBI-10220600, EBI-351935;
CC       Q8NA54; P43360: MAGEA6; NbExp=3; IntAct=EBI-10220600, EBI-1045155;
CC       Q8NA54; Q99750: MDFI; NbExp=3; IntAct=EBI-10220600, EBI-724076;
CC       Q8NA54; Q9HBE1-4: PATZ1; NbExp=3; IntAct=EBI-10220600, EBI-11022007;
CC       Q8NA54; Q8WWB5: PIH1D2; NbExp=3; IntAct=EBI-10220600, EBI-10232538;
CC       Q8NA54; Q9Y446: PKP3; NbExp=3; IntAct=EBI-10220600, EBI-2880227;
CC       Q8NA54; P78424: POU6F2; NbExp=3; IntAct=EBI-10220600, EBI-12029004;
CC       Q8NA54; Q9NQX0: PRDM6; NbExp=3; IntAct=EBI-10220600, EBI-11320284;
CC       Q8NA54; O60260-5: PRKN; NbExp=3; IntAct=EBI-10220600, EBI-21251460;
CC       Q8NA54; E9PI22: PRR23D1; NbExp=3; IntAct=EBI-10220600, EBI-23335840;
CC       Q8NA54; Q15669: RHOH; NbExp=3; IntAct=EBI-10220600, EBI-1244971;
CC       Q8NA54; Q9Y3C5: RNF11; NbExp=3; IntAct=EBI-10220600, EBI-396669;
CC       Q8NA54; Q8IYX7: SAXO1; NbExp=3; IntAct=EBI-10220600, EBI-3957636;
CC       Q8NA54; Q16637: SMN2; NbExp=6; IntAct=EBI-10220600, EBI-395421;
CC       Q8NA54; O60504: SORBS3; NbExp=3; IntAct=EBI-10220600, EBI-741237;
CC       Q8NA54; Q7Z699: SPRED1; NbExp=6; IntAct=EBI-10220600, EBI-5235340;
CC       Q8NA54; Q8N0S2: SYCE1; NbExp=3; IntAct=EBI-10220600, EBI-6872807;
CC       Q8NA54; O14656-2: TOR1A; NbExp=3; IntAct=EBI-10220600, EBI-25847109;
CC       Q8NA54; Q05BL1: TP53BP2; NbExp=3; IntAct=EBI-10220600, EBI-11952721;
CC       Q8NA54; Q12933: TRAF2; NbExp=3; IntAct=EBI-10220600, EBI-355744;
CC       Q8NA54; Q9Y4K3: TRAF6; NbExp=3; IntAct=EBI-10220600, EBI-359276;
CC       Q8NA54; P36406: TRIM23; NbExp=7; IntAct=EBI-10220600, EBI-740098;
CC       Q8NA54; P14373: TRIM27; NbExp=3; IntAct=EBI-10220600, EBI-719493;
CC       Q8NA54; Q8IWZ5: TRIM42; NbExp=3; IntAct=EBI-10220600, EBI-5235829;
CC       Q8NA54; Q86XT4: TRIM50; NbExp=3; IntAct=EBI-10220600, EBI-9867283;
CC       Q8NA54; Q9BYV2: TRIM54; NbExp=4; IntAct=EBI-10220600, EBI-2130429;
CC       Q8NA54; O76024: WFS1; NbExp=3; IntAct=EBI-10220600, EBI-720609;
CC       Q8NA54; P52747: ZNF143; NbExp=3; IntAct=EBI-10220600, EBI-2849334;
CC       Q8NA54; Q86VK4-3: ZNF410; NbExp=3; IntAct=EBI-10220600, EBI-11741890;
CC       Q8NA54; Q8TF50: ZNF526; NbExp=3; IntAct=EBI-10220600, EBI-11035148;
CC       Q8NA54; Q9UGI0: ZRANB1; NbExp=3; IntAct=EBI-10220600, EBI-527853;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum axoneme
CC       {ECO:0000250|UniProtKB:Q8CDK3}. Cell projection, cilium
CC       {ECO:0000250|UniProtKB:Q8CDK3}. Note=Localizes to the axoneme of sperm
CC       cells and the cilia of tracheal epithelial cells.
CC       {ECO:0000250|UniProtKB:Q8CDK3}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q8NA54-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8NA54-2; Sequence=VSP_022830, VSP_022831;
CC   -!- DISEASE: Note=Defects in IQUB may be the cause of spermatogenic failure
CC       with radial spoke defects, leading to asthenospermia and male
CC       infertility (PubMed:36355624). Sperm appear largely normal
CC       morphologically but with radial spoke defects, and their motility is
CC       severely reduced (PubMed:36355624). {ECO:0000269|PubMed:36355624}.
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DR   EMBL; AK093153; BAC04074.1; -; mRNA.
DR   EMBL; AK093393; BAC04153.1; -; mRNA.
DR   EMBL; CH236947; EAL24336.1; -; Genomic_DNA.
DR   EMBL; BC026173; AAH26173.1; -; mRNA.
DR   EMBL; BC036121; AAH36121.1; -; mRNA.
DR   EMBL; BC091520; AAH91520.1; -; mRNA.
DR   EMBL; BC128181; AAI28182.1; -; mRNA.
DR   CCDS; CCDS5787.1; -. [Q8NA54-1]
DR   RefSeq; NP_001269784.1; NM_001282855.1. [Q8NA54-1]
DR   RefSeq; NP_001308222.1; NM_001321293.1. [Q8NA54-1]
DR   RefSeq; NP_849149.3; NM_178827.4. [Q8NA54-1]
DR   RefSeq; XP_005250218.1; XM_005250161.3. [Q8NA54-1]
DR   RefSeq; XP_011514135.1; XM_011515833.2. [Q8NA54-1]
DR   RefSeq; XP_011514136.1; XM_011515834.2. [Q8NA54-1]
DR   RefSeq; XP_016867260.1; XM_017011771.1. [Q8NA54-1]
DR   PDB; 2DAF; NMR; -; A=123-227.
DR   PDB; 8J07; EM; 4.10 A; c2=1-791.
DR   PDBsum; 2DAF; -.
DR   PDBsum; 8J07; -.
DR   AlphaFoldDB; Q8NA54; -.
DR   EMDB; EMD-35888; -.
DR   SMR; Q8NA54; -.
DR   BioGRID; 127562; 79.
DR   ComplexPortal; CPX-8164; Radial spoke complex, flagellar variant.
DR   IntAct; Q8NA54; 76.
DR   MINT; Q8NA54; -.
DR   STRING; 9606.ENSP00000417769; -.
DR   iPTMnet; Q8NA54; -.
DR   PhosphoSitePlus; Q8NA54; -.
DR   BioMuta; IQUB; -.
DR   DMDM; 125950212; -.
DR   EPD; Q8NA54; -.
DR   MassIVE; Q8NA54; -.
DR   PaxDb; 9606-ENSP00000417769; -.
DR   PeptideAtlas; Q8NA54; -.
DR   ProteomicsDB; 72638; -. [Q8NA54-1]
DR   ProteomicsDB; 72639; -. [Q8NA54-2]
DR   Antibodypedia; 17626; 24 antibodies from 10 providers.
DR   DNASU; 154865; -.
DR   Ensembl; ENST00000324698.11; ENSP00000324882.6; ENSG00000164675.11. [Q8NA54-1]
DR   Ensembl; ENST00000466202.5; ENSP00000417769.1; ENSG00000164675.11. [Q8NA54-1]
DR   Ensembl; ENST00000484508.5; ENSP00000417285.1; ENSG00000164675.11. [Q8NA54-2]
DR   GeneID; 154865; -.
DR   KEGG; hsa:154865; -.
DR   MANE-Select; ENST00000324698.11; ENSP00000324882.6; NM_178827.5; NP_849149.3.
DR   UCSC; uc003vkn.4; human. [Q8NA54-1]
DR   AGR; HGNC:21995; -.
DR   CTD; 154865; -.
DR   DisGeNET; 154865; -.
DR   GeneCards; IQUB; -.
DR   HGNC; HGNC:21995; IQUB.
DR   HPA; ENSG00000164675; Tissue enriched (testis).
DR   MIM; 620557; gene.
DR   neXtProt; NX_Q8NA54; -.
DR   OpenTargets; ENSG00000164675; -.
DR   PharmGKB; PA162392258; -.
DR   VEuPathDB; HostDB:ENSG00000164675; -.
DR   eggNOG; ENOG502QRQT; Eukaryota.
DR   GeneTree; ENSGT00390000014326; -.
DR   HOGENOM; CLU_014415_0_0_1; -.
DR   InParanoid; Q8NA54; -.
DR   OMA; WKYIHLR; -.
DR   OrthoDB; 5478818at2759; -.
DR   PhylomeDB; Q8NA54; -.
DR   TreeFam; TF323180; -.
DR   PathwayCommons; Q8NA54; -.
DR   SignaLink; Q8NA54; -.
DR   BioGRID-ORCS; 154865; 12 hits in 1143 CRISPR screens.
DR   ChiTaRS; IQUB; human.
DR   EvolutionaryTrace; Q8NA54; -.
DR   GenomeRNAi; 154865; -.
DR   Pharos; Q8NA54; Tdark.
DR   PRO; PR:Q8NA54; -.
DR   Proteomes; UP000005640; Chromosome 7.
DR   RNAct; Q8NA54; Protein.
DR   Bgee; ENSG00000164675; Expressed in sperm and 101 other cell types or tissues.
DR   ExpressionAtlas; Q8NA54; baseline and differential.
DR   GO; GO:0097729; C:9+2 motile cilium; ISS:UniProtKB.
DR   GO; GO:0001669; C:acrosomal vesicle; IBA:GO_Central.
DR   GO; GO:0031514; C:motile cilium; IBA:GO_Central.
DR   GO; GO:0001534; C:radial spoke; ISS:UniProtKB.
DR   GO; GO:0036126; C:sperm flagellum; ISS:UniProtKB.
DR   GO; GO:0060271; P:cilium assembly; IBA:GO_Central.
DR   GO; GO:0030317; P:flagellated sperm motility; IMP:UniProtKB.
DR   GO; GO:0007618; P:mating; IMP:UniProtKB.
DR   GO; GO:0007224; P:smoothened signaling pathway; IEA:Ensembl.
DR   CDD; cd17061; Ubl_IQUB; 1.
DR   InterPro; IPR037695; IQUB.
DR   InterPro; IPR000626; Ubiquitin-like_dom.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR21074:SF0; IQ AND UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR21074; UNCHARACTERIZED; 1.
DR   Pfam; PF00240; ubiquitin; 1.
DR   SUPFAM; SSF54236; Ubiquitin-like; 1.
DR   PROSITE; PS50053; UBIQUITIN_2; 1.
DR   Genevisible; Q8NA54; HS.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; Cell projection; Cilium;
KW   Cilium biogenesis/degradation; Cytoplasm; Cytoskeleton; Disease variant;
KW   Flagellum; Reference proteome.
FT   CHAIN           1..791
FT                   /note="IQ motif and ubiquitin-like domain-containing
FT                   protein"
FT                   /id="PRO_0000274601"
FT   DOMAIN          131..207
FT                   /note="Ubiquitin-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT   DOMAIN          338..367
FT                   /note="IQ"
FT   REGION          1..73
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..23
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        42..61
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         587..680
FT                   /note="VPQDPLKFYKKIYFCHSCQLYLPSTEFSVSSTSRRIYRCRNCINLQNEAQKR
FT                   ESFLKYKCLLQQLYYTEADYEDDSKIAFLMQLQDIQYLTENI -> MGGEYLSMAL
FT                   (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_022830"
FT   VAR_SEQ         681..791
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_022831"
FT   VARIANT         126
FT                   /note="V -> M (in dbSNP:rs10255061)"
FT                   /evidence="ECO:0000269|PubMed:15489334"
FT                   /id="VAR_030332"
FT   VARIANT         314..791
FT                   /note="Missing (found in a patient with spermatogenic
FT                   failure with radial spoke defects; binding to RSPH3 and
FT                   calmodulin is abrogated; dbSNP:rs1418690066)"
FT                   /evidence="ECO:0000269|PubMed:36355624"
FT                   /id="VAR_087798"
FT   VARIANT         691
FT                   /note="D -> N (in dbSNP:rs17146009)"
FT                   /id="VAR_030333"
FT   VARIANT         735
FT                   /note="R -> H (in a colorectal cancer sample; somatic
FT                   mutation; dbSNP:rs1525626)"
FT                   /evidence="ECO:0000269|PubMed:16959974"
FT                   /id="VAR_036291"
FT   VARIANT         735
FT                   /note="R -> P (in dbSNP:rs1525626)"
FT                   /id="VAR_030334"
FT   CONFLICT        4
FT                   /note="Q -> R (in Ref. 1; BAC04074)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        252
FT                   /note="F -> S (in Ref. 1; BAC04074)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        343
FT                   /note="I -> T (in Ref. 1; BAC04074)"
FT                   /evidence="ECO:0000305"
FT   STRAND          131..137
FT                   /evidence="ECO:0007829|PDB:2DAF"
FT   TURN            138..140
FT                   /evidence="ECO:0007829|PDB:2DAF"
FT   STRAND          143..148
FT                   /evidence="ECO:0007829|PDB:2DAF"
FT   STRAND          150..152
FT                   /evidence="ECO:0007829|PDB:2DAF"
FT   HELIX           154..165
FT                   /evidence="ECO:0007829|PDB:2DAF"
FT   TURN            169..171
FT                   /evidence="ECO:0007829|PDB:2DAF"
FT   STRAND          172..176
FT                   /evidence="ECO:0007829|PDB:2DAF"
FT   STRAND          179..181
FT                   /evidence="ECO:0007829|PDB:2DAF"
FT   HELIX           187..190
FT                   /evidence="ECO:0007829|PDB:2DAF"
FT   STRAND          197..205
FT                   /evidence="ECO:0007829|PDB:2DAF"
FT   TURN            207..209
FT                   /evidence="ECO:0007829|PDB:2DAF"
SQ   SEQUENCE   791 AA;  92581 MW;  3D16F8BF4BAED522 CRC64;
     MSNQQEKYEA QNIVNSTEES DDAFDTVTIP VPSEEPQESD QTEEHESGIE QFSESHAIHV
     EEQSDQSFSS LEPDNEQLME EVISPRQVSY TPQHHEKQYA MQRPNDDSLA FLDKIKSVKE
     SLQESVEDSL ATVKVVLIPV GQEIVIPFKV DTILKYLKDH FSHLLGIPHS VLQIRYSGKI
     LKNNETLVQH GVKPQEIVQV EIFSTNPDLY PVRRIDGLTD VSQIITVTVQ TGLDQYQQVP
     VEIVKSDFHK PFLGGFRHKV TGVEYHNAGT QTVPKRIPER LSIFCRDTQT VFQKKNLQQT
     TNTTSTQMTN IGVYVSNMTD KLVTPGKYFS AAEYHAQRLK AVIVIQTYYR QWHAKIFVEN
     LRRQKSLRLE WETQQELRKI REKEEWIKLD YHRRHNPKTN EDFEFLYNAL EFWRQEELTR
     INQSFTGAER KAALCELLEK ETQIIASIGR HRYIAYMANQ EAAIQAFLDK CSAPKIWRTP
     NGKTIEMDTQ FTIRARELQN IYKCIMLKNI SQDERLDVLL TLKHTVKEHE CKLTQEILEL
     IDREVDLMMR GVKHHNLEGL RKRIATLFFH YIKTPLFNPE VAKYLKVPQD PLKFYKKIYF
     CHSCQLYLPS TEFSVSSTSR RIYRCRNCIN LQNEAQKRES FLKYKCLLQQ LYYTEADYED
     DSKIAFLMQL QDIQYLTENI WASQSVLSAC DNLSDLVMVR WNKSLEWSPW NCILLTKDEA
     AAHLKLTSIE EGYERSFIHK IKHKHILAKN YFSQVPVLAS FILDDGEIDE IRWKYHSDTT
     PKIIESQRPP H
//