Q8N9V3 (WSDU1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WD repeat, SAM and U-box domain-containing protein 1

Gene names

Name:	WDSUB1
Synonyms:	WDSAM1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Contains 1 SAM (sterile alpha motif) domain. Contains 1 U-box domain. Contains 7 WD repeats.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	ubiquitin ligase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ubiquitin-protein ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N9V3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N9V3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     226-317: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	WD repeat, SAM and U-box domain-containing protein 1		PRO_0000278608
Regions
	Repeat	10 – 47	38	WD 1
	Repeat	52 – 91	40	WD 2
	Repeat	95 – 134	40	WD 3
	Repeat	137 – 176	40	WD 4
	Repeat	178 – 228	51	WD 5
	Repeat	237 – 276	40	WD 6
	Repeat	279 – 318	40	WD 7
	Domain	332 – 396	65	SAM
	Domain	403 – 476	74	U-box
Amino acid modifications
	Modified residue	458	1	Phosphothreonine Ref.4
Natural variations
	Alternative sequence	226 – 317	92	Missing in isoform 2.		VSP_023337
	Natural variant	215	1	K → T. Corresponds to variant rs16843852 [ dbSNP | Ensembl ].		VAR_030791
	Natural variant	223	1	H → D. Ref.3 Corresponds to variant rs17852677 [ dbSNP | Ensembl ].		VAR_030792
	Natural variant	320	1	R → S. Ref.1 Corresponds to variant rs7591849 [ dbSNP | Ensembl ].		VAR_030793
Experimental info
	Sequence conflict	173	1	P → L in BAC04184. Ref.1
	Sequence conflict	173	1	P → L in AAH29520. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 5, 2009. Version 3. Checksum: F53C643A768B6622	FASTA	476	52,817
	10 20 30 40 50 60 MVKLIHTLAD HGDDVNCCAF SFSLLATCSL DKTIRLYSLR DFTELPHSPL KFHTYAVHCC 70 80 90 100 110 120 CFSPSGHILA SCSTDGTTVL WNTENGQMLA VMEQPSGSPV RVCQFSPDST CLASGAADGT 130 140 150 160 170 180 VVLWNAQSYK LYRCGSVKDG SLAACAFSPN GSFFVTGSSC GDLTVWDDKM RCPHSEKAHD 190 200 210 220 230 240 LGITCCDFSS QPVSDGEQGL QFFRLASCGQ DCQVKIWIVS FTHILGFELK YKSTLSGHCA 250 260 270 280 290 300 PVLACAFSHD GQMLVSGSVD KSVIVYDTNT ENILHTLTQH TRYVTTCAFA PNTLLLATGS 310 320 330 340 350 360 MDKTVNIWQF DLETLCQARR TEHQLKQFTE DWSEEDVSTW LCAQDLKDLV GIFKMNNIDG 370 380 390 400 410 420 KELLNLTKES LADDLKIESL GLRSKVLRKI EELRTKVKSL SSGIPDEFIC PITRELMKDP 430 440 450 460 470 VIASDGYSYE KEAMENWISK KKRTSPMTNL VLPSAVLTPN RTLKMAINRW LETHQK
	Isoform 2 [UniParc]. Checksum: D23E018257ED0EBA	FASTA	384	42,722
	10 20 30 40 50 60 MVKLIHTLAD HGDDVNCCAF SFSLLATCSL DKTIRLYSLR DFTELPHSPL KFHTYAVHCC 70 80 90 100 110 120 CFSPSGHILA SCSTDGTTVL WNTENGQMLA VMEQPSGSPV RVCQFSPDST CLASGAADGT 130 140 150 160 170 180 VVLWNAQSYK LYRCGSVKDG SLAACAFSPN GSFFVTGSSC GDLTVWDDKM RCPHSEKAHD 190 200 210 220 230 240 LGITCCDFSS QPVSDGEQGL QFFRLASCGQ DCQVKIWIVS FTHILARRTE HQLKQFTEDW 250 260 270 280 290 300 SEEDVSTWLC AQDLKDLVGI FKMNNIDGKE LLNLTKESLA DDLKIESLGL RSKVLRKIEE 310 320 330 340 350 360 LRTKVKSLSS GIPDEFICPI TRELMKDPVI ASDGYSYEKE AMENWISKKK RTSPMTNLVL 370 380 PSAVLTPNRT LKMAINRWLE THQK

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-320. Tissue: Testis.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ASP-223. Tissue: Brain.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-458, MASS SPECTROMETRY. Tissue: Cervix carcinoma.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK093494 mRNA. Translation: BAC04184.1. AC008277 Genomic DNA. No translation available. AC009307 Genomic DNA. Translation: AAX93043.1. BC029520 mRNA. Translation: AAH29520.1.
IPI	IPI00167822. IPI00477952.
RefSeq	NP_001121684.1. NM_001128212.1. NP_689741.2. NM_152528.2.
UniGene	Hs.20848.
3D structure databases
ProteinModelPortal	Q8N9V3.
ModBase	Search...
PTM databases
PhosphoSite	Q8N9V3.
Polymorphism databases
DMDM	229463016.
Proteomic databases
PaxDb	Q8N9V3.
PRIDE	Q8N9V3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000358147; ENSP00000350866; ENSG00000196151. ENST00000359774; ENSP00000352820; ENSG00000196151. ENST00000392796; ENSP00000376545; ENSG00000196151. ENST00000409990; ENSP00000387078; ENSG00000196151.
GeneID	151525.
KEGG	hsa:151525.
UCSC	uc010foo.3. human.
Organism-specific databases
CTD	151525.
GeneCards	GC02M160092.
HGNC	HGNC:26697. WDSUB1.
HPA	HPA036840.
neXtProt	NX_Q8N9V3.
PharmGKB	PA128394761.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2319.
HOGENOM	HOG000231980.
HOVERGEN	HBG080511.
InParanoid	Q8N9V3.
OMA	HCCCFSP.
OrthoDB	EOG4NCMCJ.
PhylomeDB	Q8N9V3.
Enzyme and pathway databases
SignaLink	Q8N9V3.
Gene expression databases
ArrayExpress	Q8N9V3.
Bgee	Q8N9V3.
CleanEx	HS_WDSUB1.
Genevestigator	Q8N9V3.
Family and domain databases
Gene3D	1.10.150.50. 1 hit. 2.130.10.10. 2 hits. 3.30.40.10. 1 hit.
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR001660. SAM. IPR013761. SAM/pointed. IPR011510. SAM_2. IPR003613. Ubox_domain. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF07647. SAM_2. 1 hit. PF04564. U-box. 1 hit. PF00400. WD40. 7 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00454. SAM. 1 hit. SM00504. Ubox. 1 hit. SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF47769. SAM_homology. 1 hit. SSF50978. WD40_like. 1 hit.
PROSITE	PS50105. SAM_DOMAIN. 1 hit. PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 5 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	151525.
NextBio	86717.

Entry information

Entry name

WSDU1_HUMAN

Accession

Primary (citable) accession number: Q8N9V3
Secondary accession number(s): Q53TI9, Q8N6N8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	May 5, 2009
Last modified:	May 29, 2013
This is version 87 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.