Q8N9R8 (SCAI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein SCAI

Alternative name(s):
Suppressor of cancer cell invasion protein

Gene names

Name:	SCAI
Synonyms:	C9orf126

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	606 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tumor suppressor which functions to suppress MKL1-induced SRF transcriptional activity. May function in the RHOA-DIAPH1 signal transduction pathway and regulate cell migration through transcriptional regulation of ITGB1. Ref.5
Subunit structure	Interacts with DIAPH1 By similarity. Forms a nuclear ternary complex with MKL1 and SRF. Ref.5
Subcellular location	Membrane; Single-pass membrane protein Potential. Nucleus. Cytoplasm. Note: Nuclear localization is required for inhibition of MKL1 By similarity. Ref.5
Sequence similarities	Belongs to the SCAI family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Membrane Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	Repressor Signal transduction inhibitor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of Rho protein signal transduction Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell migration Inferred from mutant phenotype Ref.5. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transcription corepressor activity Inferred from mutant phenotype Ref.5. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N9R8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N9R8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     33-33: R → SIRGSGDSSHSQSQGERYQHFTEK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 606	606	Protein SCAI		PRO_0000089735
Regions
	Transmembrane	472 – 492	21	Helical; Potential
	Region	1 – 212	212	Necessary to inhibit MKL1-induced SRF transcriptional activity By similarity
	Region	71 – 173	103	Required for interaction with MKL1 By similarity
Amino acid modifications
	Modified residue	64	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	33	1	R → SIRGSGDSSHSQSQGERYQH FTEK in isoform 2.		VSP_015120
	Natural variant	37	1	A → T. Corresponds to variant rs589292 [ dbSNP | Ensembl ].		VAR_023236
Experimental info
	Sequence conflict	432	1	I → F in BAC05217. Ref.1
	Sequence conflict	553	1	M → T in BAC05217. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 7, 2009. Version 2. Checksum: 08711C37BF37DB96	FASTA	606	70,399
	10 20 30 40 50 60 MVRGARQPQQ PRSRLAPRLT GTVEKPPRKR RSRTEFALKE IMSSGGAEDD IPQGERKTVT 70 80 90 100 110 120 DFCYLLDKSK QLFNGLRDLP QYGQKQWQSY FGRTFDVYTK LWKFQQQHRQ VLDNRYGLKR 130 140 150 160 170 180 WQIGEIASKI GQLYYHYYLR TSETSYLNEA FSFYSAIRQR SYYSQVNKED RPELVVKKLR 190 200 210 220 230 240 YYARFIVVCL LLNKMDVVKD LVKELSDEIE DYTHRFNTED QVEWNLVLQE VAAFIEADPV 250 260 270 280 290 300 MVLNDDNTIV ITSNRLAETG APLLEQGMIV GQLSLADALI IGNCNNQVKF SELTVDMFRM 310 320 330 340 350 360 LQALEREPMN LASQMNKPGM QESADKPTRR ENPHKYLLYK PTFSQLYTFL AASFKELPAN 370 380 390 400 410 420 SVLLIYLSAT GVFPTGRSDS EGPYDFGGVL TNSNRDIING DAIHKRNQSH KEMHCLHPGD 430 440 450 460 470 480 LYPFTRKPLF IIVDSSNSVA YKNFTNLFGQ PLVCLLSPTA YPKALQDQSQ RGSLFTLFLN 490 500 510 520 530 540 NPLMAFLFVS GLSSMRRGLW EKCQEYLRKI NRDIAQLLTH SRSIDQAFLQ FFGDEFLRLL 550 560 570 580 590 600 LTRFIFCSAT MRMHKIFRET RNYPESYPQL PRDETVENPH LQKHILELAS ILDVRNVFFE NTIDDY
	Isoform 2 [UniParc]. Checksum: 2DDECE3A6E61C8A4	FASTA	629	72,934
	10 20 30 40 50 60 MVRGARQPQQ PRSRLAPRLT GTVEKPPRKR RSSIRGSGDS SHSQSQGERY QHFTEKTEFA 70 80 90 100 110 120 LKEIMSSGGA EDDIPQGERK TVTDFCYLLD KSKQLFNGLR DLPQYGQKQW QSYFGRTFDV 130 140 150 160 170 180 YTKLWKFQQQ HRQVLDNRYG LKRWQIGEIA SKIGQLYYHY YLRTSETSYL NEAFSFYSAI 190 200 210 220 230 240 RQRSYYSQVN KEDRPELVVK KLRYYARFIV VCLLLNKMDV VKDLVKELSD EIEDYTHRFN 250 260 270 280 290 300 TEDQVEWNLV LQEVAAFIEA DPVMVLNDDN TIVITSNRLA ETGAPLLEQG MIVGQLSLAD 310 320 330 340 350 360 ALIIGNCNNQ VKFSELTVDM FRMLQALERE PMNLASQMNK PGMQESADKP TRRENPHKYL 370 380 390 400 410 420 LYKPTFSQLY TFLAASFKEL PANSVLLIYL SATGVFPTGR SDSEGPYDFG GVLTNSNRDI 430 440 450 460 470 480 INGDAIHKRN QSHKEMHCLH PGDLYPFTRK PLFIIVDSSN SVAYKNFTNL FGQPLVCLLS 490 500 510 520 530 540 PTAYPKALQD QSQRGSLFTL FLNNPLMAFL FVSGLSSMRR GLWEKCQEYL RKINRDIAQL 550 560 570 580 590 600 LTHSRSIDQA FLQFFGDEFL RLLLTRFIFC SATMRMHKIF RETRNYPESY PQLPRDETVE 610 620 NPHLQKHILE LASILDVRNV FFENTIDDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Thymus.
[2]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[5]	"SCAI acts as a suppressor of cancer cell invasion through the transcriptional control of beta1-integrin." Brandt D.T., Baarlink C., Kitzing T.M., Kremmer E., Ivaska J., Nollau P., Grosse R. Nat. Cell Biol. 11:557-568(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MKL1 AND SRF, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK098024 mRNA. Translation: BAC05217.1. AL445930, AL451125 Genomic DNA. Translation: CAI13674.1. AL451125, AL445930 Genomic DNA. Translation: CAI14348.1. CH471090 Genomic DNA. Translation: EAW87608.1. CH471090 Genomic DNA. Translation: EAW87609.1. BC104030 mRNA. Translation: AAI04031.1. BC104031 mRNA. Translation: AAI04032.1.
IPI	IPI00184376. IPI00646433.
RefSeq	NP_001138349.1. NM_001144877.2. NP_775961.2. NM_173690.4.
UniGene	Hs.59504.
3D structure databases
ProteinModelPortal	Q8N9R8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N9R8. 1 interaction.
STRING	9606.ENSP00000362650.
PTM databases
PhosphoSite	Q8N9R8.
Polymorphism databases
DMDM	251757332.
Proteomic databases
PaxDb	Q8N9R8.
PRIDE	Q8N9R8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000336505; ENSP00000336756; ENSG00000173611. ENST00000373549; ENSP00000362650; ENSG00000173611.
GeneID	286205.
KEGG	hsa:286205.
UCSC	uc004bpd.3. human. uc004bpe.3. human.
Organism-specific databases
CTD	286205.
GeneCards	GC09M127704.
HGNC	HGNC:26709. SCAI.
HPA	HPA020632.
neXtProt	NX_Q8N9R8.
PharmGKB	PA165586233.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG83372.
HOGENOM	HOG000234356.
OMA	RGARQPQ.
OrthoDB	EOG41NTKR.
Gene expression databases
ArrayExpress	Q8N9R8.
Bgee	Q8N9R8.
CleanEx	HS_C9orf126.
Genevestigator	Q8N9R8.
Family and domain databases
InterPro	IPR022709. DUF3550. IPR016607. UCP013022. [Graphical view]
Pfam	PF12070. DUF3550. 1 hit. [Graphical view]
PIRSF	PIRSF013022. UCP013022. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	286205.
NextBio	96078.

Entry information

Entry name

SCAI_HUMAN

Accession

Primary (citable) accession number: Q8N9R8
Secondary accession number(s): Q3SXZ1 , Q3SXZ2, Q5T163, Q8N1I4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 7, 2009
Last modified:	April 3, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.