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Protein

Protein SCAI

Gene

SCAI

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tumor suppressor which functions to suppress MKL1-induced SRF transcriptional activity. May function in the RHOA-DIAPH1 signal transduction pathway and regulate cell migration through transcriptional regulation of ITGB1.1 Publication

GO - Molecular functioni

  • transcription corepressor activity Source: UniProtKB

GO - Biological processi

  • negative regulation of cell migration Source: UniProtKB
  • negative regulation of nucleic acid-templated transcription Source: GOC
  • negative regulation of Rho protein signal transduction Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor, Signal transduction inhibitor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-5663220. RHO GTPases Activate Formins.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SCAI
Alternative name(s):
Suppressor of cancer cell invasion protein
Gene namesi
Name:SCAI
Synonyms:C9orf126
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:26709. SCAI.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei472 – 49221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165586233.

Polymorphism and mutation databases

BioMutaiSCAI.
DMDMi251757332.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 606606Protein SCAIPRO_0000089735Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8N9R8.
MaxQBiQ8N9R8.
PaxDbiQ8N9R8.
PRIDEiQ8N9R8.

PTM databases

iPTMnetiQ8N9R8.
PhosphoSiteiQ8N9R8.

Expressioni

Gene expression databases

BgeeiQ8N9R8.
CleanExiHS_C9orf126.
ExpressionAtlasiQ8N9R8. baseline and differential.
GenevisibleiQ8N9R8. HS.

Organism-specific databases

HPAiHPA020632.

Interactioni

Subunit structurei

Interacts with DIAPH1 (By similarity). Forms a nuclear ternary complex with MKL1 and SRF.By similarity1 Publication

Protein-protein interaction databases

BioGridi130333. 8 interactions.
IntActiQ8N9R8. 4 interactions.
STRINGi9606.ENSP00000362650.

Structurei

3D structure databases

ProteinModelPortaliQ8N9R8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 212212Necessary to inhibit MKL1-induced SRF transcriptional activityBy similarityAdd
BLAST
Regioni71 – 173103Required for interaction with MKL1By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the SCAI family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEKH. Eukaryota.
ENOG410XRRG. LUCA.
GeneTreeiENSGT00390000009566.
HOGENOMiHOG000234356.
InParanoidiQ8N9R8.
OMAiCQDYLRK.
OrthoDBiEOG74BJRD.
PhylomeDBiQ8N9R8.
TreeFamiTF324872.

Family and domain databases

InterProiIPR022709. SCAI.
IPR016607. SCAI_metazoan/Viridiplantae.
[Graphical view]
PfamiPF12070. DUF3550. 1 hit.
[Graphical view]
PIRSFiPIRSF013022. UCP013022. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N9R8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVRGARQPQQ PRSRLAPRLT GTVEKPPRKR RSRTEFALKE IMSSGGAEDD
60 70 80 90 100
IPQGERKTVT DFCYLLDKSK QLFNGLRDLP QYGQKQWQSY FGRTFDVYTK
110 120 130 140 150
LWKFQQQHRQ VLDNRYGLKR WQIGEIASKI GQLYYHYYLR TSETSYLNEA
160 170 180 190 200
FSFYSAIRQR SYYSQVNKED RPELVVKKLR YYARFIVVCL LLNKMDVVKD
210 220 230 240 250
LVKELSDEIE DYTHRFNTED QVEWNLVLQE VAAFIEADPV MVLNDDNTIV
260 270 280 290 300
ITSNRLAETG APLLEQGMIV GQLSLADALI IGNCNNQVKF SELTVDMFRM
310 320 330 340 350
LQALEREPMN LASQMNKPGM QESADKPTRR ENPHKYLLYK PTFSQLYTFL
360 370 380 390 400
AASFKELPAN SVLLIYLSAT GVFPTGRSDS EGPYDFGGVL TNSNRDIING
410 420 430 440 450
DAIHKRNQSH KEMHCLHPGD LYPFTRKPLF IIVDSSNSVA YKNFTNLFGQ
460 470 480 490 500
PLVCLLSPTA YPKALQDQSQ RGSLFTLFLN NPLMAFLFVS GLSSMRRGLW
510 520 530 540 550
EKCQEYLRKI NRDIAQLLTH SRSIDQAFLQ FFGDEFLRLL LTRFIFCSAT
560 570 580 590 600
MRMHKIFRET RNYPESYPQL PRDETVENPH LQKHILELAS ILDVRNVFFE

NTIDDY
Length:606
Mass (Da):70,399
Last modified:July 7, 2009 - v2
Checksum:i08711C37BF37DB96
GO
Isoform 2 (identifier: Q8N9R8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     33-33: R → SIRGSGDSSHSQSQGERYQHFTEK

Show »
Length:629
Mass (Da):72,934
Checksum:i2DDECE3A6E61C8A4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti432 – 4321I → F in BAC05217 (PubMed:14702039).Curated
Sequence conflicti553 – 5531M → T in BAC05217 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti37 – 371A → T.
Corresponds to variant rs589292 [ dbSNP | Ensembl ].
VAR_023236

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei33 – 331R → SIRGSGDSSHSQSQGERYQH FTEK in isoform 2. 2 PublicationsVSP_015120

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK098024 mRNA. Translation: BAC05217.1.
AL445930, AL451125 Genomic DNA. Translation: CAI13674.1.
AL451125, AL445930 Genomic DNA. Translation: CAI14348.1.
CH471090 Genomic DNA. Translation: EAW87608.1.
CH471090 Genomic DNA. Translation: EAW87609.1.
BC104030 mRNA. Translation: AAI04031.1.
BC104031 mRNA. Translation: AAI04032.1.
CCDSiCCDS43877.1. [Q8N9R8-2]
CCDS48017.1. [Q8N9R8-1]
RefSeqiNP_001138349.1. NM_001144877.2. [Q8N9R8-1]
NP_775961.2. NM_173690.4. [Q8N9R8-2]
UniGeneiHs.59504.

Genome annotation databases

EnsembliENST00000336505; ENSP00000336756; ENSG00000173611. [Q8N9R8-1]
ENST00000373549; ENSP00000362650; ENSG00000173611. [Q8N9R8-2]
GeneIDi286205.
KEGGihsa:286205.
UCSCiuc004bpd.4. human. [Q8N9R8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK098024 mRNA. Translation: BAC05217.1.
AL445930, AL451125 Genomic DNA. Translation: CAI13674.1.
AL451125, AL445930 Genomic DNA. Translation: CAI14348.1.
CH471090 Genomic DNA. Translation: EAW87608.1.
CH471090 Genomic DNA. Translation: EAW87609.1.
BC104030 mRNA. Translation: AAI04031.1.
BC104031 mRNA. Translation: AAI04032.1.
CCDSiCCDS43877.1. [Q8N9R8-2]
CCDS48017.1. [Q8N9R8-1]
RefSeqiNP_001138349.1. NM_001144877.2. [Q8N9R8-1]
NP_775961.2. NM_173690.4. [Q8N9R8-2]
UniGeneiHs.59504.

3D structure databases

ProteinModelPortaliQ8N9R8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi130333. 8 interactions.
IntActiQ8N9R8. 4 interactions.
STRINGi9606.ENSP00000362650.

PTM databases

iPTMnetiQ8N9R8.
PhosphoSiteiQ8N9R8.

Polymorphism and mutation databases

BioMutaiSCAI.
DMDMi251757332.

Proteomic databases

EPDiQ8N9R8.
MaxQBiQ8N9R8.
PaxDbiQ8N9R8.
PRIDEiQ8N9R8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336505; ENSP00000336756; ENSG00000173611. [Q8N9R8-1]
ENST00000373549; ENSP00000362650; ENSG00000173611. [Q8N9R8-2]
GeneIDi286205.
KEGGihsa:286205.
UCSCiuc004bpd.4. human. [Q8N9R8-1]

Organism-specific databases

CTDi286205.
GeneCardsiSCAI.
HGNCiHGNC:26709. SCAI.
HPAiHPA020632.
neXtProtiNX_Q8N9R8.
PharmGKBiPA165586233.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEKH. Eukaryota.
ENOG410XRRG. LUCA.
GeneTreeiENSGT00390000009566.
HOGENOMiHOG000234356.
InParanoidiQ8N9R8.
OMAiCQDYLRK.
OrthoDBiEOG74BJRD.
PhylomeDBiQ8N9R8.
TreeFamiTF324872.

Enzyme and pathway databases

ReactomeiR-HSA-5663220. RHO GTPases Activate Formins.

Miscellaneous databases

GenomeRNAii286205.
PROiQ8N9R8.

Gene expression databases

BgeeiQ8N9R8.
CleanExiHS_C9orf126.
ExpressionAtlasiQ8N9R8. baseline and differential.
GenevisibleiQ8N9R8. HS.

Family and domain databases

InterProiIPR022709. SCAI.
IPR016607. SCAI_metazoan/Viridiplantae.
[Graphical view]
PfamiPF12070. DUF3550. 1 hit.
[Graphical view]
PIRSFiPIRSF013022. UCP013022. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Thymus.
  2. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
  5. "SCAI acts as a suppressor of cancer cell invasion through the transcriptional control of beta1-integrin."
    Brandt D.T., Baarlink C., Kitzing T.M., Kremmer E., Ivaska J., Nollau P., Grosse R.
    Nat. Cell Biol. 11:557-568(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MKL1 AND SRF, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSCAI_HUMAN
AccessioniPrimary (citable) accession number: Q8N9R8
Secondary accession number(s): Q3SXZ1
, Q3SXZ2, Q5T163, Q8N1I4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: July 7, 2009
Last modified: June 8, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.