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Q8N9I9 (DTX3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable E3 ubiquitin-protein ligase DTX3

EC=6.3.2.-
Alternative name(s):
Protein deltex-3
Short name=Deltex3
RING finger protein 154
Gene names
Name:DTX3
Synonyms:RNF154
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context By similarity. Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Homodimer. May form a heterodimers with other members of the Deltex family. Interacts with NOTCH1 By similarity. Ref.1

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the Deltex family.

Contains 1 RING-type zinc finger.

Sequence caution

The sequence CAD38593.2 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
   Biological processNotch signaling pathway
Ubl conjugation pathway
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNotch signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionligase activity

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8N9I9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8N9I9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: MSFVLSR → MPILSSSGSK
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 347347Probable E3 ubiquitin-protein ligase DTX3
PRO_0000219085

Regions

Zinc finger164 – 20542RING-type
Compositional bias122 – 15130Pro-rich

Natural variations

Alternative sequence1 – 77MSFVLSR → MPILSSSGSK in isoform 2.
VSP_008354

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 26, 2003. Version 2.
Checksum: FDB4CDC982F1B707

FASTA34737,988
        10         20         30         40         50         60 
MSFVLSRMAA CGGTCKNKVT VSKPVWDFLS KETPARLARL REEHRVSILI DGETSDIYVL 

        70         80         90        100        110        120 
QLSPQGPPPA PPNGLYLARK ALKGLLKEAE KELKKAQRQG ELMGCLALGG GGEHPEMHRA 

       130        140        150        160        170        180 
GPPPLRAAPL LPPGARGLPP PPPPLPPPLP PRLREEAEEQ ESTCPICLGE IQNAKTLEKC 

       190        200        210        220        230        240 
RHSFCEGCIT RALQVKKACP MCGRFYGQLV GNQPQNGRML VSKDATLLLP SYEKYGTIVI 

       250        260        270        280        290        300 
QYVFPPGVQG AEHPNPGVRY PGTTRVAYLP DCPEGNKVLT LFRKAFDQRL TFTIGTSMTT 

       310        320        330        340 
GRPNVITWND IHHKTSCTGG PQLFGYPDPT YLTRVQEELR AKGITDD 

« Hide

Isoform 2 [UniParc].

Checksum: 41ACDC6F899F99F3
Show »

FASTA35038,155

References

« Hide 'large scale' references
[1]"The BAL-binding protein BBAP and related Deltex family members exhibit ubiquitin-protein isopeptide ligase activity."
Takeyama K., Aguiar R.C.T., Gu L., He C., Freeman G.J., Kutok J.L., Aster J.C., Shipp M.A.
J. Biol. Chem. 278:21930-21937(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), IN VITRO UBIQUITIN LIGASE ACTIVITY, SUBUNIT.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Cerebellum and Teratocarcinoma.
[3]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-201 (ISOFORM 1).
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY225126 mRNA. Translation: AAP57520.1.
AK092085 mRNA. Translation: BAC03801.1.
AK094385 mRNA. Translation: BAC04344.1.
AK128752 mRNA. Translation: BAG54727.1.
CH471054 Genomic DNA. Translation: EAW97033.1.
BC114441 mRNA. Translation: AAI14442.1.
BC114498 mRNA. Translation: AAI14499.1.
AL831941 mRNA. Translation: CAD38593.2. Sequence problems.
CCDSCCDS41800.1. [Q8N9I9-1]
CCDS66410.1. [Q8N9I9-2]
RefSeqNP_001273174.1. NM_001286245.1. [Q8N9I9-2]
NP_001273175.1. NM_001286246.1. [Q8N9I9-1]
NP_848597.1. NM_178502.3. [Q8N9I9-1]
XP_005268754.1. XM_005268697.1. [Q8N9I9-2]
XP_005268755.1. XM_005268698.1. [Q8N9I9-2]
XP_005268757.1. XM_005268700.1. [Q8N9I9-2]
XP_005268760.1. XM_005268703.1. [Q8N9I9-1]
UniGeneHs.32374.

3D structure databases

ProteinModelPortalQ8N9I9.
SMRQ8N9I9. Positions 164-202, 211-344.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid128203. 23 interactions.
IntActQ8N9I9. 20 interactions.
STRING9606.ENSP00000338050.

PTM databases

PhosphoSiteQ8N9I9.

Polymorphism databases

DMDM37077338.

Proteomic databases

MaxQBQ8N9I9.
PaxDbQ8N9I9.
PRIDEQ8N9I9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000337737; ENSP00000338050; ENSG00000178498. [Q8N9I9-1]
ENST00000548198; ENSP00000447873; ENSG00000178498. [Q8N9I9-1]
ENST00000548478; ENSP00000448224; ENSG00000178498.
ENST00000548804; ENSP00000449294; ENSG00000178498. [Q8N9I9-1]
ENST00000551632; ENSP00000448696; ENSG00000178498. [Q8N9I9-2]
GeneID196403.
KEGGhsa:196403.
UCSCuc001sov.1. human. [Q8N9I9-1]

Organism-specific databases

CTD196403.
GeneCardsGC12P057998.
HGNCHGNC:24457. DTX3.
HPAHPA059654.
MIM613142. gene.
neXtProtNX_Q8N9I9.
PharmGKBPA134887936.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG84763.
HOGENOMHOG000007352.
HOVERGENHBG045495.
InParanoidQ8N9I9.
KOK06058.
OMACEDCITR.
OrthoDBEOG7QNVKH.
PhylomeDBQ8N9I9.
TreeFamTF325526.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ8N9I9.
BgeeQ8N9I9.
CleanExHS_DTX3.
GenevestigatorQ8N9I9.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamPF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTSM00184. RING. 1 hit.
[Graphical view]
PROSITEPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi196403.
NextBio89461.
PROQ8N9I9.
SOURCESearch...

Entry information

Entry nameDTX3_HUMAN
AccessionPrimary (citable) accession number: Q8N9I9
Secondary accession number(s): Q53ZZ2, Q8NAU6, Q8NDS8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: September 26, 2003
Last modified: July 9, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM