Q8N9I9 (DTX3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable E3 ubiquitin-protein ligase DTX3
EC=6.3.2.-

Alternative name(s):
Protein deltex-3
Short name=Deltex3
RING finger protein 154

Gene names

Name:	DTX3
Synonyms:	RNF154

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context By similarity. Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Homodimer. May form a heterodimers with other members of the Deltex family. Interacts with NOTCH1 By similarity. Ref.1
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the Deltex family. Contains 1 RING-type zinc finger.
Sequence caution	The sequence CAD38593.2 differs from that shown. Reason: Intron retention.

Ontologies

Keywords
Biological process	Notch signaling pathway Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N9I9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N9I9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: MSFVLSR → MPILSSSGSK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Probable E3 ubiquitin-protein ligase DTX3		PRO_0000219085
Regions
	Zinc finger	164 – 205	42	RING-type
	Compositional bias	122 – 151	30	Pro-rich
Natural variations
	Alternative sequence	1 – 7	7	MSFVLSR → MPILSSSGSK in isoform 2.		VSP_008354

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 26, 2003. Version 2. Checksum: FDB4CDC982F1B707	FASTA	347	37,988
	10 20 30 40 50 60 MSFVLSRMAA CGGTCKNKVT VSKPVWDFLS KETPARLARL REEHRVSILI DGETSDIYVL 70 80 90 100 110 120 QLSPQGPPPA PPNGLYLARK ALKGLLKEAE KELKKAQRQG ELMGCLALGG GGEHPEMHRA 130 140 150 160 170 180 GPPPLRAAPL LPPGARGLPP PPPPLPPPLP PRLREEAEEQ ESTCPICLGE IQNAKTLEKC 190 200 210 220 230 240 RHSFCEGCIT RALQVKKACP MCGRFYGQLV GNQPQNGRML VSKDATLLLP SYEKYGTIVI 250 260 270 280 290 300 QYVFPPGVQG AEHPNPGVRY PGTTRVAYLP DCPEGNKVLT LFRKAFDQRL TFTIGTSMTT 310 320 330 340 GRPNVITWND IHHKTSCTGG PQLFGYPDPT YLTRVQEELR AKGITDD
	Isoform 2 [UniParc]. Checksum: 41ACDC6F899F99F3	FASTA	350	38,155
	10 20 30 40 50 60 MPILSSSGSK MAACGGTCKN KVTVSKPVWD FLSKETPARL ARLREEHRVS ILIDGETSDI 70 80 90 100 110 120 YVLQLSPQGP PPAPPNGLYL ARKALKGLLK EAEKELKKAQ RQGELMGCLA LGGGGEHPEM 130 140 150 160 170 180 HRAGPPPLRA APLLPPGARG LPPPPPPLPP PLPPRLREEA EEQESTCPIC LGEIQNAKTL 190 200 210 220 230 240 EKCRHSFCEG CITRALQVKK ACPMCGRFYG QLVGNQPQNG RMLVSKDATL LLPSYEKYGT 250 260 270 280 290 300 IVIQYVFPPG VQGAEHPNPG VRYPGTTRVA YLPDCPEGNK VLTLFRKAFD QRLTFTIGTS 310 320 330 340 350 MTTGRPNVIT WNDIHHKTSC TGGPQLFGYP DPTYLTRVQE ELRAKGITDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The BAL-binding protein BBAP and related Deltex family members exhibit ubiquitin-protein isopeptide ligase activity." Takeyama K., Aguiar R.C.T., Gu L., He C., Freeman G.J., Kutok J.L., Aster J.C., Shipp M.A. J. Biol. Chem. 278:21930-21937(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), IN VITRO UBIQUITIN LIGASE ACTIVITY, SUBUNIT.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Cerebellum and Teratocarcinoma.
[3]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-201 (ISOFORM 1). Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY225126 mRNA. Translation: AAP57520.1. AK092085 mRNA. Translation: BAC03801.1. AK094385 mRNA. Translation: BAC04344.1. AK128752 mRNA. Translation: BAG54727.1. CH471054 Genomic DNA. Translation: EAW97033.1. BC114441 mRNA. Translation: AAI14442.1. BC114498 mRNA. Translation: AAI14499.1. AL831941 mRNA. Translation: CAD38593.2. Sequence problems.
IPI	IPI00148820. IPI00184064.
RefSeq	NP_848597.1. NM_178502.2.
UniGene	Hs.32374.
3D structure databases
ProteinModelPortal	Q8N9I9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N9I9. 19 interactions.
STRING	9606.ENSP00000338050.
PTM databases
PhosphoSite	Q8N9I9.
Polymorphism databases
DMDM	37077338.
Proteomic databases
PaxDb	Q8N9I9.
PRIDE	Q8N9I9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000337737; ENSP00000338050; ENSG00000178498. ENST00000548198; ENSP00000447873; ENSG00000178498. ENST00000548478; ENSP00000448224; ENSG00000178498. ENST00000548804; ENSP00000449294; ENSG00000178498. ENST00000551632; ENSP00000448696; ENSG00000178498.
GeneID	196403.
KEGG	hsa:196403.
UCSC	uc001sov.1. human.
Organism-specific databases
CTD	196403.
GeneCards	GC12P057998.
HGNC	HGNC:24457. DTX3.
MIM	613142. gene.
neXtProt	NX_Q8N9I9.
PharmGKB	PA134887936.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG84763.
HOGENOM	HOG000007352.
HOVERGEN	HBG045495.
InParanoid	Q8N9I9.
KO	K06058.
OMA	GPQLCDS.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q8N9I9.
Bgee	Q8N9I9.
CleanEx	HS_DTX3.
Genevestigator	Q8N9I9.
GermOnline	ENSG00000178498. Homo sapiens.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR017907. Znf_RING_CS. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	196403.
NextBio	89461.
SOURCE	Search...

Entry information

Entry name

DTX3_HUMAN

Accession

Primary (citable) accession number: Q8N9I9
Secondary accession number(s): Q53ZZ2, Q8NAU6, Q8NDS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	September 26, 2003
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.