Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Synaptotagmin-2

Gene

SYT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone (By similarity).By similarity

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi169 – 1691Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi170 – 1701Calcium 1By similarity
Metal bindingi170 – 1701Calcium 2By similarity
Metal bindingi176 – 1761Calcium 1By similarity
Metal bindingi228 – 2281Calcium 1By similarity
Metal bindingi228 – 2281Calcium 2By similarity
Metal bindingi229 – 2291Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi230 – 2301Calcium 1By similarity
Metal bindingi230 – 2301Calcium 2By similarity
Metal bindingi230 – 2301Calcium 3By similarity
Metal bindingi233 – 2331Calcium 3By similarity
Metal bindingi234 – 2341Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi236 – 2361Calcium 2By similarity
Metal bindingi236 – 2361Calcium 3By similarity

GO - Molecular functioni

  1. calcium-dependent phospholipid binding Source: Ensembl
  2. calcium ion binding Source: Ensembl
  3. transporter activity Source: InterPro

GO - Biological processi

  1. pathogenesis Source: Reactome
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_264609. Toxicity of botulinum toxin type B (BoNT/B).

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-2
Alternative name(s):
Synaptotagmin II
Short name:
SytII
Gene namesi
Name:SYT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:11510. SYT2.

Subcellular locationi

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane By similarity; Single-pass membrane protein By similarity. Cytoplasmic vesiclesecretory vesiclechromaffin granule membrane By similarity; Single-pass membrane protein By similarity
Note: Synaptic vesicles and chromaffin granules.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6262VesicularSequence AnalysisAdd
BLAST
Transmembranei63 – 8321HelicalSequence AnalysisAdd
BLAST
Topological domaini84 – 419336CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. chromaffin granule membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. intracellular membrane-bounded organelle Source: HPA
  5. plasma membrane Source: Reactome
  6. synaptic vesicle membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

Pathology & Biotechi

Involvement in diseasei

Myasthenic syndrome, presynaptic, congenital, with or without motor neuropathy (MYSPC)1 Publication

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionAn autosomal dominant presynaptic neuromuscular junction disorder resembling Lambert-Eaton myasthenic syndrome. Affected individuals show foot deformities, a variable degree of proximal and distal limb weakness, muscle fatigue that improves with rest, mild gait difficulties, and reduced or absent deep tendon reflexes.

See also OMIM:616040
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti307 – 3071D → A in MYSPC. 1 Publication
VAR_072578
Natural varianti308 – 3081P → L in MYSPC. 1 Publication
VAR_072579

Keywords - Diseasei

Congenital myasthenic syndrome, Disease mutation

Organism-specific databases

MIMi616040. phenotype.
PharmGKBiPA36291.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419Synaptotagmin-2PRO_0000183942Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
Modified residuei227 – 2271PhosphotyrosineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8N9I0.
PRIDEiQ8N9I0.

PTM databases

PhosphoSiteiQ8N9I0.

Expressioni

Gene expression databases

BgeeiQ8N9I0.
CleanExiHS_SYT2.
ExpressionAtlasiQ8N9I0. baseline and differential.
GenevestigatoriQ8N9I0.

Organism-specific databases

HPAiHPA030372.

Interactioni

Subunit structurei

Homotetramer (Probable). Interacts with stonin 2 (By similarity). Interacts with SCAMP5.By similarityCurated1 Publication

Protein-protein interaction databases

BioGridi126085. 5 interactions.
IntActiQ8N9I0. 4 interactions.
MINTiMINT-2810903.
STRINGi9606.ENSP00000356236.

Structurei

3D structure databases

ProteinModelPortaliQ8N9I0.
SMRiQ8N9I0. Positions 138-416.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini141 – 242102C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini272 – 375104C2 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 379247Phospholipid bindingBy similarityAdd
BLAST

Domaini

The first C2 domain mediates Ca2+-dependent phospholipid binding.By similarity
The second C2 domain mediates interaction with Stonin 2.By similarity

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5038.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232127.
HOVERGENiHBG005010.
InParanoidiQ8N9I0.
OMAiTHHSKFR.
OrthoDBiEOG78PV8W.
PhylomeDBiQ8N9I0.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR030543. Syt2.
[Graphical view]
PANTHERiPTHR10024:SF208. PTHR10024:SF208. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8N9I0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNIFKRNQE PIVAPATTTA TMPIGPVDNS TESGGAGESQ EDMFAKLKEK
60 70 80 90 100
LFNEINKIPL PPWALIAIAV VAGLLLLTCC FCICKKCCCK KKKNKKEKGK
110 120 130 140 150
GMKNAMNMKD MKGGQDDDDA ETGLTEGEGE GEEEKEPENL GKLQFSLDYD
160 170 180 190 200
FQANQLTVGV LQAAELPALD MGGTSDPYVK VFLLPDKKKK YETKVHRKTL
210 220 230 240 250
NPAFNETFTF KVPYQELGGK TLVMAIYDFD RFSKHDIIGE VKVPMNTVDL
260 270 280 290 300
GQPIEEWRDL QGGEKEEPEK LGDICTSLRY VPTAGKLTVC ILEAKNLKKM
310 320 330 340 350
DVGGLSDPYV KIHLMQNGKR LKKKKTTVKK KTLNPYFNES FSFEIPFEQI
360 370 380 390 400
QKVQVVVTVL DYDKLGKNEA IGKIFVGSNA TGTELRHWSD MLANPRRPIA
410
QWHSLKPEEE VDALLGKNK
Length:419
Mass (Da):46,872
Last modified:October 17, 2006 - v2
Checksum:iBE3855E9CDE2D76E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti310 – 3101V → G in BAC04354 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti307 – 3071D → A in MYSPC. 1 Publication
VAR_072578
Natural varianti308 – 3081P → L in MYSPC. 1 Publication
VAR_072579

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090672 mRNA. Translation: BAC03500.1.
AK094430 mRNA. Translation: BAC04354.1.
BC100814 mRNA. Translation: AAI00815.1.
BC100815 mRNA. Translation: AAI00816.1.
BC100817 mRNA. Translation: AAI00818.1.
CCDSiCCDS1427.1.
RefSeqiNP_001129976.1. NM_001136504.1.
NP_796376.2. NM_177402.4.
UniGeneiHs.25422.

Genome annotation databases

EnsembliENST00000367267; ENSP00000356236; ENSG00000143858.
ENST00000367268; ENSP00000356237; ENSG00000143858.
GeneIDi127833.
KEGGihsa:127833.
UCSCiuc001gye.3. human.

Polymorphism databases

DMDMi116242811.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090672 mRNA. Translation: BAC03500.1.
AK094430 mRNA. Translation: BAC04354.1.
BC100814 mRNA. Translation: AAI00815.1.
BC100815 mRNA. Translation: AAI00816.1.
BC100817 mRNA. Translation: AAI00818.1.
CCDSiCCDS1427.1.
RefSeqiNP_001129976.1. NM_001136504.1.
NP_796376.2. NM_177402.4.
UniGeneiHs.25422.

3D structure databases

ProteinModelPortaliQ8N9I0.
SMRiQ8N9I0. Positions 138-416.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126085. 5 interactions.
IntActiQ8N9I0. 4 interactions.
MINTiMINT-2810903.
STRINGi9606.ENSP00000356236.

Chemistry

DrugBankiDB00042. Botulinum Toxin Type B.

PTM databases

PhosphoSiteiQ8N9I0.

Polymorphism databases

DMDMi116242811.

Proteomic databases

PaxDbiQ8N9I0.
PRIDEiQ8N9I0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367267; ENSP00000356236; ENSG00000143858.
ENST00000367268; ENSP00000356237; ENSG00000143858.
GeneIDi127833.
KEGGihsa:127833.
UCSCiuc001gye.3. human.

Organism-specific databases

CTDi127833.
GeneCardsiGC01M202559.
HGNCiHGNC:11510. SYT2.
HPAiHPA030372.
MIMi600104. gene.
616040. phenotype.
neXtProtiNX_Q8N9I0.
PharmGKBiPA36291.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5038.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232127.
HOVERGENiHBG005010.
InParanoidiQ8N9I0.
OMAiTHHSKFR.
OrthoDBiEOG78PV8W.
PhylomeDBiQ8N9I0.
TreeFamiTF315600.

Enzyme and pathway databases

ReactomeiREACT_264609. Toxicity of botulinum toxin type B (BoNT/B).

Miscellaneous databases

ChiTaRSiSYT2. human.
GeneWikiiSYT2.
GenomeRNAii127833.
NextBioi82195.
PROiQ8N9I0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8N9I0.
CleanExiHS_SYT2.
ExpressionAtlasiQ8N9I0. baseline and differential.
GenevestigatoriQ8N9I0.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR030543. Syt2.
[Graphical view]
PANTHERiPTHR10024:SF208. PTHR10024:SF208. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Cerebellum.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Human SCAMP5, a novel secretory carrier membrane protein, facilitates calcium-triggered cytokine secretion by interaction with SNARE machinery."
    Han C., Chen T., Yang M., Li N., Liu H., Cao X.
    J. Immunol. 182:2986-2996(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SCAMP5.
  4. "Synaptotagmin 2 mutations cause an autosomal-dominant form of lambert-eaton myasthenic syndrome and nonprogressive motor neuropathy."
    Herrmann D.N., Horvath R., Sowden J.E., Gonzales M., Sanchez-Mejias A., Guan Z., Whittaker R.G., Almodovar J.L., Lane M., Bansagi B., Pyle A., Boczonadi V., Lochmuller H., Griffin H., Chinnery P.F., Lloyd T.E., Littleton J.T., Zuchner S.
    Am. J. Hum. Genet. 95:332-339(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INVOLVEMENT IN MYSPC, VARIANTS MYSPC ALA-307 AND LEU-308.

Entry informationi

Entry nameiSYT2_HUMAN
AccessioniPrimary (citable) accession number: Q8N9I0
Secondary accession number(s): Q496K5, Q8NBE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: October 17, 2006
Last modified: April 1, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.