Reviewed, UniProtKB/Swiss-Prot Q8N9I0 (SYT2_HUMAN)

Names and origin

Protein names

Recommended name:
    Synaptotagmin-2
Alternative name(s):
    Synaptotagmin II
      Short name=SytII

Gene names

Name:

SYT2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone By similarity.
Cofactor	Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.
Subunit structure	Homotetramer Probable. Interacts with stonin 2 By similarity. Interacts with SCAMP5. Ref.3
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass membrane protein By similarity. Cytoplasmic vesicle › secretory vesicle › chromaffin granule membrane; Single-pass membrane protein By similarity. Note: Synaptic vesicles and chromaffin granules By similarity.
Domain	The first C2 domain mediates Ca2+-dependent phospholipid binding By similarity. The second C2 domain mediates interaction with Stonin 2 By similarity.
Sequence similarities	Belongs to the synaptotagmin family. Contains 2 C2 domains.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse
Domain	Repeat Transmembrane
Ligand	Calcium Metal-binding
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: InterPro
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW endocytic vesicle membrane Inferred from Experiment. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synaptic vesicle Inferred from electronic annotation. Source: InterPro
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.3 Inferred from physical interaction. Source: UniProtKB transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Synaptotagmin-2		PRO_0000183942
Regions
	Topological domain	1 – 62	62	Vesicular Potential
	Transmembrane	63 – 83	21	Potential
	Topological domain	84 – 419	336	Cytoplasmic Potential
	Domain	141 – 242	102	C2 1
	Domain	272 – 375	104	C2 2
	Region	133 – 379	247	Phospholipid binding By similarity
Sites
	Metal binding	169	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	170	1	Calcium 1 By similarity
	Metal binding	170	1	Calcium 2 By similarity
	Metal binding	176	1	Calcium 1 By similarity
	Metal binding	228	1	Calcium 1 By similarity
	Metal binding	228	1	Calcium 2 By similarity
	Metal binding	229	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	230	1	Calcium 1 By similarity
	Metal binding	230	1	Calcium 2 By similarity
	Metal binding	230	1	Calcium 3 By similarity
	Metal binding	233	1	Calcium 3 By similarity
	Metal binding	234	1	Calcium 3; via carbonyl oxygen By similarity
	Metal binding	236	1	Calcium 2 By similarity
	Metal binding	236	1	Calcium 3 By similarity
Amino acid modifications
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	310	1	V → G in BAC04354. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8N9I0-1 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: BE3855E9CDE2D76E	FASTA	419	46,872
	10 20 30 40 50 60 MRNIFKRNQE PIVAPATTTA TMPIGPVDNS TESGGAGESQ EDMFAKLKEK LFNEINKIPL 70 80 90 100 110 120 PPWALIAIAV VAGLLLLTCC FCICKKCCCK KKKNKKEKGK GMKNAMNMKD MKGGQDDDDA 130 140 150 160 170 180 ETGLTEGEGE GEEEKEPENL GKLQFSLDYD FQANQLTVGV LQAAELPALD MGGTSDPYVK 190 200 210 220 230 240 VFLLPDKKKK YETKVHRKTL NPAFNETFTF KVPYQELGGK TLVMAIYDFD RFSKHDIIGE 250 260 270 280 290 300 VKVPMNTVDL GQPIEEWRDL QGGEKEEPEK LGDICTSLRY VPTAGKLTVC ILEAKNLKKM 310 320 330 340 350 360 DVGGLSDPYV KIHLMQNGKR LKKKKTTVKK KTLNPYFNES FSFEIPFEQI QKVQVVVTVL 370 380 390 400 410 DYDKLGKNEA IGKIFVGSNA TGTELRHWSD MLANPRRPIA QWHSLKPEEE VDALLGKNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Human SCAMP5, a novel secretory carrier membrane protein, facilitates calcium-triggered cytokine secretion by interaction with SNARE machinery." Han C., Chen T., Yang M., Li N., Liu H., Cao X. J. Immunol. 182:2986-2996(2009) [PubMed: 19234194] [Abstract] Cited for: INTERACTION WITH SCAMP5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK090672 mRNA. Translation: BAC03500.1. AK094430 mRNA. Translation: BAC04354.1. BC100814 mRNA. Translation: AAI00815.1. BC100815 mRNA. Translation: AAI00816.1. BC100817 mRNA. Translation: AAI00818.1.
IPI	IPI00219274.
RefSeq	NP_001129976.1. NP_796376.2.
UniGene	Hs.25422
3D structure databases
SMR	Q8N9I0. Positions 138-416.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8N9I0.
PTM databases
PhosphoSite	Q8N9I0.
Proteomic databases
PRIDE	Q8N9I0.
Genome annotation databases
Ensembl	ENST00000367267; ENSP00000356236; ENSG00000143858; Homo sapiens. [Genome view] ENST00000367268; ENSP00000356237; ENSG00000143858; Homo sapiens. [Genome view]
GeneID	127833.
KEGG	hsa:127833.
UCSC	uc001gyd.1. human.
Organism-specific databases
CTD	127833.
GeneCards	GC01M200826.
HGNC	HGNC:11510. SYT2.
MIM	600104. gene.
PharmGKB	PA36291.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG716633.
HOVERGEN	Q8N9I0.
InParanoid	Q8N9I0.
OMA	TATMPIG.
OrthoDB	EOG98D3F9.
PhylomeDB	Q8N9I0.
Enzyme and pathway databases
Reactome	REACT_11184. Botulinum neurotoxicity.
Gene expression databases
ArrayExpress	Q8N9I0.
Bgee	Q8N9I0.
CleanEx	HS_SYT2.
Genevestigator	Q8N9I0.
GermOnline	ENSG00000143858. Homo sapiens.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR020477. C2_region. IPR001565. Synaptotagmin. IPR015428. Synaptotagmin1_2. [Graphical view]
PANTHER	PTHR10024:SF39. Synaptotagmin1_2. 1 hit.
Pfam	PF00168. C2. 2 hits. [Graphical view]
PRINTS	PR00360. C2DOMAIN. PR00399. SYNAPTOTAGMN.
SMART	SM00239. C2. 2 hits. [Graphical view]
PROSITE	PS50004. C2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00042. Botulinum Toxin Type B.
NextBio	82195.
SOURCE	Search...

Entry information

Entry name

SYT2_HUMAN

Accession

Primary (citable) accession number: Q8N9I0
Secondary accession number(s): Q496K5, Q8NBE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	October 17, 2006
Last modified:	January 19, 2010
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.