Q8N9I0 (SYT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Synaptotagmin-2

Alternative name(s):
Synaptotagmin II
Short name=SytII

Gene names

Name:

SYT2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone By similarity.
Cofactor	Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.
Subunit structure	Homotetramer Probable. Interacts with stonin 2 By similarity. Interacts with SCAMP5. Ref.3
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass membrane protein By similarity. Cytoplasmic vesicle › secretory vesicle › chromaffin granule membrane; Single-pass membrane protein By similarity. Note: Synaptic vesicles and chromaffin granules By similarity.
Domain	The first C2 domain mediates Ca2+-dependent phospholipid binding By similarity. The second C2 domain mediates interaction with Stonin 2 By similarity.
Sequence similarities	Belongs to the synaptotagmin family. Contains 2 C2 domains.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse
Domain	Repeat Transmembrane Transmembrane helix
Ligand	Calcium Metal-binding
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	neurotransmitter secretion Traceable author statement. Source: Reactome
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW chromaffin granule membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endocytic vesicle membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome synaptic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: Compara calcium-dependent phospholipid binding Inferred from electronic annotation. Source: Compara transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Synaptotagmin-2		PRO_0000183942
Regions
	Topological domain	1 – 62	62	Vesicular Potential
	Transmembrane	63 – 83	21	Helical; Potential
	Topological domain	84 – 419	336	Cytoplasmic Potential
	Domain	141 – 242	102	C2 1
	Domain	272 – 375	104	C2 2
	Region	133 – 379	247	Phospholipid binding By similarity
Sites
	Metal binding	169	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	170	1	Calcium 1 By similarity
	Metal binding	170	1	Calcium 2 By similarity
	Metal binding	176	1	Calcium 1 By similarity
	Metal binding	228	1	Calcium 1 By similarity
	Metal binding	228	1	Calcium 2 By similarity
	Metal binding	229	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	230	1	Calcium 1 By similarity
	Metal binding	230	1	Calcium 2 By similarity
	Metal binding	230	1	Calcium 3 By similarity
	Metal binding	233	1	Calcium 3 By similarity
	Metal binding	234	1	Calcium 3; via carbonyl oxygen By similarity
	Metal binding	236	1	Calcium 2 By similarity
	Metal binding	236	1	Calcium 3 By similarity
Amino acid modifications
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	310	1	V → G in BAC04354. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8N9I0 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: BE3855E9CDE2D76E	FASTA	419	46,872
	10 20 30 40 50 60 MRNIFKRNQE PIVAPATTTA TMPIGPVDNS TESGGAGESQ EDMFAKLKEK LFNEINKIPL 70 80 90 100 110 120 PPWALIAIAV VAGLLLLTCC FCICKKCCCK KKKNKKEKGK GMKNAMNMKD MKGGQDDDDA 130 140 150 160 170 180 ETGLTEGEGE GEEEKEPENL GKLQFSLDYD FQANQLTVGV LQAAELPALD MGGTSDPYVK 190 200 210 220 230 240 VFLLPDKKKK YETKVHRKTL NPAFNETFTF KVPYQELGGK TLVMAIYDFD RFSKHDIIGE 250 260 270 280 290 300 VKVPMNTVDL GQPIEEWRDL QGGEKEEPEK LGDICTSLRY VPTAGKLTVC ILEAKNLKKM 310 320 330 340 350 360 DVGGLSDPYV KIHLMQNGKR LKKKKTTVKK KTLNPYFNES FSFEIPFEQI QKVQVVVTVL 370 380 390 400 410 DYDKLGKNEA IGKIFVGSNA TGTELRHWSD MLANPRRPIA QWHSLKPEEE VDALLGKNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Human SCAMP5, a novel secretory carrier membrane protein, facilitates calcium-triggered cytokine secretion by interaction with SNARE machinery." Han C., Chen T., Yang M., Li N., Liu H., Cao X. J. Immunol. 182:2986-2996(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SCAMP5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK090672 mRNA. Translation: BAC03500.1. AK094430 mRNA. Translation: BAC04354.1. BC100814 mRNA. Translation: AAI00815.1. BC100815 mRNA. Translation: AAI00816.1. BC100817 mRNA. Translation: AAI00818.1.
IPI	IPI00219274.
RefSeq	NP_001129976.1. NM_001136504.1. NP_796376.2. NM_177402.4.
UniGene	Hs.25422.
3D structure databases
ProteinModelPortal	Q8N9I0.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000356236.
PTM databases
PhosphoSite	Q8N9I0.
Polymorphism databases
DMDM	116242811.
Proteomic databases
PaxDb	Q8N9I0.
PRIDE	Q8N9I0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000367267; ENSP00000356236; ENSG00000143858. ENST00000367268; ENSP00000356237; ENSG00000143858.
GeneID	127833.
KEGG	hsa:127833.
UCSC	uc001gye.3. human.
Organism-specific databases
CTD	127833.
GeneCards	GC01M202559.
HGNC	HGNC:11510. SYT2.
HPA	HPA030372.
MIM	600104. gene.
neXtProt	NX_Q8N9I0.
PharmGKB	PA36291.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5038.
HOGENOM	HOG000232127.
HOVERGEN	HBG005010.
InParanoid	Q8N9I0.
OMA	AKNAMNM.
OrthoDB	EOG4MGS7Q.
PhylomeDB	Q8N9I0.
Enzyme and pathway databases
Reactome	REACT_116125. Disease. REACT_13685. Neuronal System.
Gene expression databases
Bgee	Q8N9I0.
CleanEx	HS_SYT2.
Genevestigator	Q8N9I0.
GermOnline	ENSG00000143858. Homo sapiens.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR020477. C2_dom. IPR018029. C2_membr_targeting. IPR001565. Synaptotagmin. [Graphical view]
Pfam	PF00168. C2. 2 hits. [Graphical view]
PRINTS	PR00360. C2DOMAIN. PR00399. SYNAPTOTAGMN.
SMART	SM00239. C2. 2 hits. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 2 hits.
PROSITE	PS50004. C2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SYT2. human.
DrugBank	DB00042. Botulinum Toxin Type B.
GenomeRNAi	127833.
NextBio	82195.
SOURCE	Search...

Entry information

Entry name

SYT2_HUMAN

Accession

Primary (citable) accession number: Q8N9I0
Secondary accession number(s): Q496K5, Q8NBE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.