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Q8N9B5

- JMY_HUMAN

UniProt

Q8N9B5 - JMY_HUMAN

Protein

Junction-mediating and -regulatory protein

Gene

JMY

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (18 Mar 2008)
      Previous versions | rss
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    Functioni

    Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. transcription coactivator activity Source: HGNC

    GO - Biological processi

    1. 'de novo' actin filament nucleation Source: UniProtKB
    2. actin polymerization-dependent cell motility Source: UniProtKB
    3. Arp2/3 complex-mediated actin nucleation Source: UniProtKB
    4. cell cycle arrest Source: UniProtKB
    5. DNA repair Source: UniProtKB-KW
    6. intrinsic apoptotic signaling pathway by p53 class mediator Source: Ensembl
    7. positive regulation of apoptotic process Source: UniProtKB
    8. positive regulation of sequence-specific DNA binding transcription factor activity Source: UniProtKB
    9. regulation of transcription from RNA polymerase II promoter Source: HGNC

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    Actin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Junction-mediating and -regulatory protein
    Gene namesi
    Name:JMY
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:28916. JMY.

    Subcellular locationi

    Nucleus By similarity. Cytoplasmcytoskeleton 1 Publication
    Note: Localizes to the nucleus in most cell types. Accumulates in nucleus under DNA damage conditions, increasing p53/TP53 transcription response and reducing its influence on cell motility By similarity. In primary neutrophils, it colocalizes with actin filaments at the leading edge and is excluded from the nucleus. Localization correlates with motility, because it moves from the nucleus to the cytoplasmic compartment when cells are differentiated from nonmotile cells into highly motile neutrophil-like cells.By similarity

    GO - Cellular componenti

    1. cell leading edge Source: UniProtKB
    2. cytoplasm Source: UniProtKB-KW
    3. cytoskeleton Source: UniProtKB-SubCell
    4. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA164721124.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 988988Junction-mediating and -regulatory proteinPRO_0000324611Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei974 – 9741Phosphoserine1 Publication

    Post-translational modificationi

    Ubiquitinated by MDM2, leading to its subsequent degradation by the proteasome. In case of DNA damage, the interaction with MDM2 is altered, preventing degradation and allowing interaction with p300/EP300 and its function in p53/TP53 stress response By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ8N9B5.
    PaxDbiQ8N9B5.
    PRIDEiQ8N9B5.

    PTM databases

    PhosphoSiteiQ8N9B5.

    Miscellaneous databases

    PMAP-CutDBQ8N9B5.

    Expressioni

    Inductioni

    By E2F.1 Publication

    Gene expression databases

    BgeeiQ8N9B5.
    GenevestigatoriQ8N9B5.

    Organism-specific databases

    HPAiHPA043308.

    Interactioni

    Subunit structurei

    Interacts with p300/EP300, the complex being recruited to activated p53/TP53. Interacts with TTC5 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MDM2Q009872EBI-866435,EBI-389668

    Protein-protein interaction databases

    BioGridi126372. 3 interactions.
    IntActiQ8N9B5. 2 interactions.
    MINTiMINT-4052528.
    STRINGi9606.ENSP00000379441.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8N9B5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini921 – 93818WH2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 126126Interaction with p300/EP300By similarityAdd
    BLAST
    Regioni478 – 56790Interaction with p300/EP300By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili324 – 36037Sequence AnalysisAdd
    BLAST
    Coiled coili489 – 54153Sequence AnalysisAdd
    BLAST
    Coiled coili590 – 62132Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi781 – 82747Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the JMY family.Curated
    Contains 1 WH2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG39317.
    HOGENOMiHOG000074181.
    HOVERGENiHBG067146.
    InParanoidiQ8N9B5.
    OMAiKFQQRLR.
    OrthoDBiEOG7QVM1Z.
    PhylomeDBiQ8N9B5.
    TreeFamiTF331023.

    Family and domain databases

    InterProiIPR003124. WH2_dom.
    [Graphical view]
    SMARTiSM00246. WH2. 1 hit.
    [Graphical view]
    PROSITEiPS51082. WH2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8N9B5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSFALEETLE SDWVAVRPHV FDEREKHKFV FIVAWNEIEG KFAITCHNRT    50
    AQRQRSGSRE QAGARGGAEA GGAASDGSRG PGSPAGRGRP EATASATLVR 100
    SPGPRRSSAW AEGGSPRSTR SLLGDPRLRS PGSKGAESRL RSPVRAKPIP 150
    GQKTSEADDA AGAAAAAARP APREAQVSSV RIVSASGTVS EEIEVLEMVK 200
    EDEAPLALSD AEQPPPATEL ESPAEECSWA GLFSFQDLRA VHQQLCSVNS 250
    QLEPCLPVFP EEPSGMWTVL FGGAPEMTEQ EIDTLCYQLQ VYLGHGLDTC 300
    GWKILSQVLF TETDDPEEYY ESLSELRQKG YEEVLQRARK RIQELLDKHK 350
    NTESMVELLD LYQMEDEAYS SLAEATTELY QYLLQPFRDM RELAMLRRQQ 400
    IKISMENDYL GPRRIESLQK EDADWQRKAH MAVLSIQDLT VKYFEITAKA 450
    QKAVYDRMRA DQKKFGKASW AAAAERMEKL QYAVSKETLQ MMRAKEICLE 500
    QRKHALKEEM QSLRGGTEAI ARLDQLEADY YDLQLQLYEV QFEILKCEEL 550
    LLTAQLESIK RLISEKRDEV VYYDTYESME AMLEKEEMAA SAYLQREELQ 600
    KLQQKARQLE ARRGRVSAKK SYLRNKKEIC IAKHNEKIQQ RTRIEDEYRT 650
    HHTVQLKREK LHDEEERKSA WVSQERQRTL DRLRTFKQRY PGQVILKSTR 700
    LRLAHARRKG AASPVLQEDH CDSLPSVLQV EEKTEEVGEG RVKRGPSQTT 750
    EPQSLVQLED TSLTQLEATS LPLSGVTSEL PPTISLPLLN NNLEPCSVTI 800
    NPLPSPLPPT PPPPPPPPPP PPPPPLPVAK DSGPETLEKD LPRKEGNEKR 850
    IPKSASAPSA HLFDSSQLVS ARKKLRKTAE GLQRRRVSSP MDEVLASLKR 900
    GSFHLKKVEQ RTLPPFPDED DSNNILAQIR KGVKLKKVQK DVLRESFTLL 950
    PDTDPLTRSI HEALRRIKEA SPESEDEEEA LPCTDWEN 988
    Length:988
    Mass (Da):111,445
    Last modified:March 18, 2008 - v2
    Checksum:i7166FF8AE325F37A
    GO
    Isoform 2 (identifier: Q8N9B5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         814-825: Missing.

    Show »
    Length:976
    Mass (Da):110,280
    Checksum:i27175E3EFA32295E
    GO

    Sequence cautioni

    The sequence AAI30625.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC04495.1 differs from that shown. Reason: Erroneous initiation.
    The sequence EAW95834.1 differs from that shown. Reason: Erroneous gene model prediction.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti364 – 3641M → L.2 Publications
    Corresponds to variant rs13182512 [ dbSNP | Ensembl ].
    VAR_039846
    Natural varianti592 – 5921A → V.
    Corresponds to variant rs12109475 [ dbSNP | Ensembl ].
    VAR_039847
    Natural varianti720 – 7201H → R.
    Corresponds to variant rs16876657 [ dbSNP | Ensembl ].
    VAR_039848

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei814 – 82512Missing in isoform 2. 1 PublicationVSP_032310Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC016559 Genomic DNA. No translation available.
    AC020898 Genomic DNA. No translation available.
    CH471084 Genomic DNA. Translation: EAW95834.1. Sequence problems.
    BC130624 mRNA. Translation: AAI30625.1. Different initiation.
    AK095189 mRNA. Translation: BAC04495.1. Different initiation.
    CCDSiCCDS4047.3. [Q8N9B5-1]
    RefSeqiNP_689618.4. NM_152405.4. [Q8N9B5-1]
    UniGeneiHs.482605.

    Genome annotation databases

    EnsembliENST00000396137; ENSP00000379441; ENSG00000152409. [Q8N9B5-1]
    GeneIDi133746.
    KEGGihsa:133746.
    UCSCiuc003kfw.1. human. [Q8N9B5-1]

    Polymorphism databases

    DMDMi172045777.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC016559 Genomic DNA. No translation available.
    AC020898 Genomic DNA. No translation available.
    CH471084 Genomic DNA. Translation: EAW95834.1 . Sequence problems.
    BC130624 mRNA. Translation: AAI30625.1 . Different initiation.
    AK095189 mRNA. Translation: BAC04495.1 . Different initiation.
    CCDSi CCDS4047.3. [Q8N9B5-1 ]
    RefSeqi NP_689618.4. NM_152405.4. [Q8N9B5-1 ]
    UniGenei Hs.482605.

    3D structure databases

    ProteinModelPortali Q8N9B5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 126372. 3 interactions.
    IntActi Q8N9B5. 2 interactions.
    MINTi MINT-4052528.
    STRINGi 9606.ENSP00000379441.

    PTM databases

    PhosphoSitei Q8N9B5.

    Polymorphism databases

    DMDMi 172045777.

    Proteomic databases

    MaxQBi Q8N9B5.
    PaxDbi Q8N9B5.
    PRIDEi Q8N9B5.

    Protocols and materials databases

    DNASUi 133746.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000396137 ; ENSP00000379441 ; ENSG00000152409 . [Q8N9B5-1 ]
    GeneIDi 133746.
    KEGGi hsa:133746.
    UCSCi uc003kfw.1. human. [Q8N9B5-1 ]

    Organism-specific databases

    CTDi 133746.
    GeneCardsi GC05P078609.
    H-InvDB HIX0004982.
    HGNCi HGNC:28916. JMY.
    HPAi HPA043308.
    MIMi 604279. gene.
    neXtProti NX_Q8N9B5.
    PharmGKBi PA164721124.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG39317.
    HOGENOMi HOG000074181.
    HOVERGENi HBG067146.
    InParanoidi Q8N9B5.
    OMAi KFQQRLR.
    OrthoDBi EOG7QVM1Z.
    PhylomeDBi Q8N9B5.
    TreeFami TF331023.

    Miscellaneous databases

    GenomeRNAii 133746.
    NextBioi 83276.
    PMAP-CutDB Q8N9B5.
    PROi Q8N9B5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8N9B5.
    Genevestigatori Q8N9B5.

    Family and domain databases

    InterProi IPR003124. WH2_dom.
    [Graphical view ]
    SMARTi SM00246. WH2. 1 hit.
    [Graphical view ]
    PROSITEi PS51082. WH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The DNA sequence and comparative analysis of human chromosome 5."
      Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
      , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
      Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT LEU-364.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 345-988 (ISOFORM 2).
      Tissue: Brain.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 351-988 (ISOFORM 1), VARIANT LEU-364.
      Tissue: Substantia nigra.
    5. "Novel link between E2F and p53: proapoptotic cofactors of p53 are transcriptionally upregulated by E2F."
      Hershko T., Chaussepied M., Oren M., Ginsberg D.
      Cell Death Differ. 12:377-383(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-974, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "p53-cofactor JMY is a multifunctional actin nucleation factor."
      Zuchero J.B., Coutts A.S., Quinlan M.E., Thangue N.B., Mullins R.D.
      Nat. Cell Biol. 11:451-459(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiJMY_HUMAN
    AccessioniPrimary (citable) accession number: Q8N9B5
    Secondary accession number(s): A1L4P5, B5MDS2, B5MDT0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3