Q8N9B5 (JMY_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified April 3, 2013. Version 82. History...
Names and origin
|Protein names||Recommended name:|
Junction-mediating and -regulatory protein
|Organism||Homo sapiens (Human) [Reference proteome]|
|Taxonomic identifier||9606 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo|
|Sequence length||988 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location By similarity.
Interacts with p300/EP300, the complex being recruited to activated p53/TP53. Interacts with TTC5 By similarity.
Nucleus By similarity. Cytoplasm › cytoskeleton. Note: Localizes to the nucleus in most cell types. Accumulates in nucleus under DNA damage conditions, increasing p53/TP53 transcription response and reducing its influence on cell motility By similarity. In primary neutrophils, it colocalizes with actin filaments at the leading edge and is excluded from the nucleus. Localization correlates with motility, because it moves from the nucleus to the cytoplasmic compartment when cells are differentiated from nonmotile cells into highly motile neutrophil-like cells. Ref.7
By E2F. Ref.5
Ubiquitinated by MDM2, leading to its subsequent degradation by the proteasome. In case of DNA damage, the interaction with MDM2 is altered, preventing degradation and allowing interaction with p300/EP300 and its function in p53/TP53 stress response By similarity.
Belongs to the JMY family.
Contains 1 WH2 domain.
The sequence AAI30625.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC04495.1 differs from that shown. Reason: Erroneous initiation.
The sequence EAW95834.1 differs from that shown. Reason: Erroneous gene model prediction.
|This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]|
|Isoform 1 (identifier: Q8N9B5-1) |
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
|Isoform 2 (identifier: Q8N9B5-2) |
The sequence of this isoform differs from the canonical sequence as follows:
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 988||988||Junction-mediating and -regulatory protein||PRO_0000324611|
|Domain||921 – 938||18||WH2|
|Region||1 – 126||126||Interaction with p300/EP300 By similarity|
|Region||478 – 567||90||Interaction with p300/EP300 By similarity|
|Coiled coil||324 – 360||37||Potential|
|Coiled coil||489 – 541||53||Potential|
|Coiled coil||590 – 621||32||Potential|
|Compositional bias||781 – 827||47||Pro-rich|
Amino acid modifications
|Modified residue||83||1||Phosphoserine By similarity|
|Modified residue||974||1||Phosphoserine Ref.6|
|Alternative sequence||814 – 825||12||Missing in isoform 2.||VSP_032310|
|Natural variant||364||1||M → L. Ref.2 Ref.4|
Corresponds to variant rs13182512 [ dbSNP | Ensembl ].
|Natural variant||592||1||A → V.|
Corresponds to variant rs12109475 [ dbSNP | Ensembl ].
|Natural variant||720||1||H → R.|
Corresponds to variant rs16876657 [ dbSNP | Ensembl ].
|AC016559 Genomic DNA. No translation available.|
AC020898 Genomic DNA. No translation available.
CH471084 Genomic DNA. Translation: EAW95834.1. Sequence problems.
BC130624 mRNA. Translation: AAI30625.1. Different initiation.
AK095189 mRNA. Translation: BAC04495.1. Different initiation.
|RefSeq||NP_689618.4. NM_152405.4. |
3D structure databases
Protein-protein interaction databases
|IntAct||Q8N9B5. 2 interactions.|
Protocols and materials databases
Genome annotation databases
|Ensembl||ENST00000396137; ENSP00000379441; ENSG00000152409. |
|UCSC||uc003kfw.1. human. |
|HGNC||HGNC:28916. JMY. |
|MIM||604279. gene. |
Gene expression databases
Family and domain databases
|InterPro||IPR003124. WH2_dom. |
|SMART||SM00246. WH2. 1 hit. |
|PROSITE||PS51082. WH2. 1 hit. |
|Accession||Primary (citable) accession number: Q8N9B5|
Secondary accession number(s): A1L4P5, B5MDS2, B5MDT0
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
|Disclaimer||Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.|
|Human chromosome 5|
Human chromosome 5: entries, gene names and cross-references to MIM
|Human entries with polymorphisms or disease mutations|
List of human entries with polymorphisms or disease mutations
|Human polymorphisms and disease mutations|
Index of human polymorphisms and disease mutations
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Index of protein domains and families