Q8N9B5 (JMY_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Junction-mediating and -regulatory protein

Gene names

Name:

JMY

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	988 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location By similarity.
Subunit structure	Interacts with p300/EP300, the complex being recruited to activated p53/TP53. Interacts with TTC5 By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm › cytoskeleton. Note: Localizes to the nucleus in most cell types. Accumulates in nucleus under DNA damage conditions, increasing p53/TP53 transcription response and reducing its influence on cell motility By similarity. In primary neutrophils, it colocalizes with actin filaments at the leading edge and is excluded from the nucleus. Localization correlates with motility, because it moves from the nucleus to the cytoplasmic compartment when cells are differentiated from nonmotile cells into highly motile neutrophil-like cells. Ref.7
Induction	By E2F. Ref.5
Post-translational modification	Ubiquitinated by MDM2, leading to its subsequent degradation by the proteasome. In case of DNA damage, the interaction with MDM2 is altered, preventing degradation and allowing interaction with p300/EP300 and its function in p53/TP53 stress response By similarity.
Sequence similarities	Belongs to the JMY family. Contains 1 WH2 domain.
Sequence caution	The sequence AAI30625.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC04495.1 differs from that shown. Reason: Erroneous initiation. The sequence EAW95834.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Cytoplasm Cytoskeleton Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Ligand	Actin-binding
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' actin filament nucleation Inferred from sequence or structural similarity. Source: UniProtKB Arp2/3 complex-mediated actin nucleation Inferred from sequence or structural similarity. Source: UniProtKB DNA repair Inferred from electronic annotation. Source: UniProtKB-KW actin polymerization-dependent cell motility Inferred from sequence or structural similarity. Source: UniProtKB cell cycle arrest Inferred from sequence or structural similarity. Source: UniProtKB induction of apoptosis Inferred from sequence or structural similarity. Source: HGNC positive regulation of sequence-specific DNA binding transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: HGNC
Cellular_component	cell leading edge Inferred from direct assay Ref.7. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	transcription coactivator activity Inferred from sequence or structural similarity. Source: HGNC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MDM2	Q00987	2	EBI-866435,EBI-389668

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N9B5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N9B5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     814-825: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 988	988	Junction-mediating and -regulatory protein		PRO_0000324611
Regions
	Domain	921 – 938	18	WH2
	Region	1 – 126	126	Interaction with p300/EP300 By similarity
	Region	478 – 567	90	Interaction with p300/EP300 By similarity
	Coiled coil	324 – 360	37	Potential
	Coiled coil	489 – 541	53	Potential
	Coiled coil	590 – 621	32	Potential
	Compositional bias	781 – 827	47	Pro-rich
Amino acid modifications
	Modified residue	83	1	Phosphoserine By similarity
	Modified residue	974	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	814 – 825	12	Missing in isoform 2.		VSP_032310
	Natural variant	364	1	M → L. Ref.2 Ref.4 Corresponds to variant rs13182512 [ dbSNP | Ensembl ].		VAR_039846
	Natural variant	592	1	A → V. Corresponds to variant rs12109475 [ dbSNP | Ensembl ].		VAR_039847
	Natural variant	720	1	H → R. Corresponds to variant rs16876657 [ dbSNP | Ensembl ].		VAR_039848

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 7166FF8AE325F37A	FASTA	988	111,445
	10 20 30 40 50 60 MSFALEETLE SDWVAVRPHV FDEREKHKFV FIVAWNEIEG KFAITCHNRT AQRQRSGSRE 70 80 90 100 110 120 QAGARGGAEA GGAASDGSRG PGSPAGRGRP EATASATLVR SPGPRRSSAW AEGGSPRSTR 130 140 150 160 170 180 SLLGDPRLRS PGSKGAESRL RSPVRAKPIP GQKTSEADDA AGAAAAAARP APREAQVSSV 190 200 210 220 230 240 RIVSASGTVS EEIEVLEMVK EDEAPLALSD AEQPPPATEL ESPAEECSWA GLFSFQDLRA 250 260 270 280 290 300 VHQQLCSVNS QLEPCLPVFP EEPSGMWTVL FGGAPEMTEQ EIDTLCYQLQ VYLGHGLDTC 310 320 330 340 350 360 GWKILSQVLF TETDDPEEYY ESLSELRQKG YEEVLQRARK RIQELLDKHK NTESMVELLD 370 380 390 400 410 420 LYQMEDEAYS SLAEATTELY QYLLQPFRDM RELAMLRRQQ IKISMENDYL GPRRIESLQK 430 440 450 460 470 480 EDADWQRKAH MAVLSIQDLT VKYFEITAKA QKAVYDRMRA DQKKFGKASW AAAAERMEKL 490 500 510 520 530 540 QYAVSKETLQ MMRAKEICLE QRKHALKEEM QSLRGGTEAI ARLDQLEADY YDLQLQLYEV 550 560 570 580 590 600 QFEILKCEEL LLTAQLESIK RLISEKRDEV VYYDTYESME AMLEKEEMAA SAYLQREELQ 610 620 630 640 650 660 KLQQKARQLE ARRGRVSAKK SYLRNKKEIC IAKHNEKIQQ RTRIEDEYRT HHTVQLKREK 670 680 690 700 710 720 LHDEEERKSA WVSQERQRTL DRLRTFKQRY PGQVILKSTR LRLAHARRKG AASPVLQEDH 730 740 750 760 770 780 CDSLPSVLQV EEKTEEVGEG RVKRGPSQTT EPQSLVQLED TSLTQLEATS LPLSGVTSEL 790 800 810 820 830 840 PPTISLPLLN NNLEPCSVTI NPLPSPLPPT PPPPPPPPPP PPPPPLPVAK DSGPETLEKD 850 860 870 880 890 900 LPRKEGNEKR IPKSASAPSA HLFDSSQLVS ARKKLRKTAE GLQRRRVSSP MDEVLASLKR 910 920 930 940 950 960 GSFHLKKVEQ RTLPPFPDED DSNNILAQIR KGVKLKKVQK DVLRESFTLL PDTDPLTRSI 970 980 HEALRRIKEA SPESEDEEEA LPCTDWEN
	Isoform 2 [UniParc]. Checksum: 27175E3EFA32295E	FASTA	976	110,280
	10 20 30 40 50 60 MSFALEETLE SDWVAVRPHV FDEREKHKFV FIVAWNEIEG KFAITCHNRT AQRQRSGSRE 70 80 90 100 110 120 QAGARGGAEA GGAASDGSRG PGSPAGRGRP EATASATLVR SPGPRRSSAW AEGGSPRSTR 130 140 150 160 170 180 SLLGDPRLRS PGSKGAESRL RSPVRAKPIP GQKTSEADDA AGAAAAAARP APREAQVSSV 190 200 210 220 230 240 RIVSASGTVS EEIEVLEMVK EDEAPLALSD AEQPPPATEL ESPAEECSWA GLFSFQDLRA 250 260 270 280 290 300 VHQQLCSVNS QLEPCLPVFP EEPSGMWTVL FGGAPEMTEQ EIDTLCYQLQ VYLGHGLDTC 310 320 330 340 350 360 GWKILSQVLF TETDDPEEYY ESLSELRQKG YEEVLQRARK RIQELLDKHK NTESMVELLD 370 380 390 400 410 420 LYQMEDEAYS SLAEATTELY QYLLQPFRDM RELAMLRRQQ IKISMENDYL GPRRIESLQK 430 440 450 460 470 480 EDADWQRKAH MAVLSIQDLT VKYFEITAKA QKAVYDRMRA DQKKFGKASW AAAAERMEKL 490 500 510 520 530 540 QYAVSKETLQ MMRAKEICLE QRKHALKEEM QSLRGGTEAI ARLDQLEADY YDLQLQLYEV 550 560 570 580 590 600 QFEILKCEEL LLTAQLESIK RLISEKRDEV VYYDTYESME AMLEKEEMAA SAYLQREELQ 610 620 630 640 650 660 KLQQKARQLE ARRGRVSAKK SYLRNKKEIC IAKHNEKIQQ RTRIEDEYRT HHTVQLKREK 670 680 690 700 710 720 LHDEEERKSA WVSQERQRTL DRLRTFKQRY PGQVILKSTR LRLAHARRKG AASPVLQEDH 730 740 750 760 770 780 CDSLPSVLQV EEKTEEVGEG RVKRGPSQTT EPQSLVQLED TSLTQLEATS LPLSGVTSEL 790 800 810 820 830 840 PPTISLPLLN NNLEPCSVTI NPLPSPLPPT PPPLPVAKDS GPETLEKDLP RKEGNEKRIP 850 860 870 880 890 900 KSASAPSAHL FDSSQLVSAR KKLRKTAEGL QRRRVSSPMD EVLASLKRGS FHLKKVEQRT 910 920 930 940 950 960 LPPFPDEDDS NNILAQIRKG VKLKKVQKDV LRESFTLLPD TDPLTRSIHE ALRRIKEASP 970 ESEDEEEALP CTDWEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT LEU-364.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 345-988 (ISOFORM 2). Tissue: Brain.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 351-988 (ISOFORM 1), VARIANT LEU-364. Tissue: Substantia nigra.
[5]	"Novel link between E2F and p53: proapoptotic cofactors of p53 are transcriptionally upregulated by E2F." Hershko T., Chaussepied M., Oren M., Ginsberg D. Cell Death Differ. 12:377-383(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-974, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"p53-cofactor JMY is a multifunctional actin nucleation factor." Zuchero J.B., Coutts A.S., Quinlan M.E., Thangue N.B., Mullins R.D. Nat. Cell Biol. 11:451-459(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC016559 Genomic DNA. No translation available. AC020898 Genomic DNA. No translation available. CH471084 Genomic DNA. Translation: EAW95834.1. Sequence problems. BC130624 mRNA. Translation: AAI30625.1. Different initiation. AK095189 mRNA. Translation: BAC04495.1. Different initiation.
IPI	IPI00182739. IPI00889171.
RefSeq	NP_689618.4. NM_152405.4.
UniGene	Hs.482605.
3D structure databases
ProteinModelPortal	Q8N9B5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N9B5. 2 interactions.
MINT	MINT-4052528.
STRING	9606.ENSP00000379441.
PTM databases
PhosphoSite	Q8N9B5.
Polymorphism databases
DMDM	172045777.
Proteomic databases
PaxDb	Q8N9B5.
PRIDE	Q8N9B5.
Protocols and materials databases
DNASU	133746.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000396137; ENSP00000379441; ENSG00000152409.
GeneID	133746.
KEGG	hsa:133746.
UCSC	uc003kfw.1. human.
Organism-specific databases
CTD	133746.
GeneCards	GC05P078609.
H-InvDB	HIX0004982.
HGNC	HGNC:28916. JMY.
HPA	HPA043308.
MIM	604279. gene.
neXtProt	NX_Q8N9B5.
PharmGKB	PA164721124.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG39317.
HOGENOM	HOG000074181.
HOVERGEN	HBG067146.
InParanoid	Q8N9B5.
OMA	KFQQRLR.
OrthoDB	EOG4MW85R.
Gene expression databases
ArrayExpress	Q8N9B5.
Bgee	Q8N9B5.
Genevestigator	Q8N9B5.
Family and domain databases
InterPro	IPR003124. WH2_dom. [Graphical view]
SMART	SM00246. WH2. 1 hit. [Graphical view]
PROSITE	PS51082. WH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	133746.
NextBio	83276.
PMAP-CutDB	Q8N9B5.
SOURCE	Search...

Entry information

Entry name

JMY_HUMAN

Accession

Primary (citable) accession number: Q8N9B5
Secondary accession number(s): A1L4P5, B5MDS2, B5MDT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	April 3, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.