Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Junction-mediating and -regulatory protein

Gene

JMY

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000152409-MONOMER.
ReactomeiR-HSA-6804760. Regulation of TP53 Activity through Methylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Junction-mediating and -regulatory protein
Gene namesi
Name:JMY
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:28916. JMY.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasmcytoskeleton 1 Publication

  • Note: Localizes to the nucleus in most cell types. Accumulates in nucleus under DNA damage conditions, increasing p53/TP53 transcription response and reducing its influence on cell motility (By similarity). In primary neutrophils, it colocalizes with actin filaments at the leading edge and is excluded from the nucleus. Localization correlates with motility, because it moves from the nucleus to the cytoplasmic compartment when cells are differentiated from nonmotile cells into highly motile neutrophil-like cells.By similarity

GO - Cellular componenti

  • cell leading edge Source: UniProtKB
  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: UniProtKB-SubCell
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi133746.
OpenTargetsiENSG00000152409.
PharmGKBiPA164721124.

Polymorphism and mutation databases

BioMutaiJMY.
DMDMi172045777.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003246111 – 988Junction-mediating and -regulatory proteinAdd BLAST988

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei115PhosphoserineCombined sources1
Modified residuei121PhosphoserineCombined sources1
Modified residuei713PhosphoserineCombined sources1
Modified residuei888PhosphoserineBy similarity1
Modified residuei974PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated by MDM2, leading to its subsequent degradation by the proteasome. In case of DNA damage, the interaction with MDM2 is altered, preventing degradation and allowing interaction with p300/EP300 and its function in p53/TP53 stress response (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8N9B5.
MaxQBiQ8N9B5.
PaxDbiQ8N9B5.
PeptideAtlasiQ8N9B5.
PRIDEiQ8N9B5.

PTM databases

iPTMnetiQ8N9B5.
PhosphoSitePlusiQ8N9B5.

Miscellaneous databases

PMAP-CutDBQ8N9B5.

Expressioni

Inductioni

By E2F.1 Publication

Gene expression databases

BgeeiENSG00000152409.
GenevisibleiQ8N9B5. HS.

Organism-specific databases

HPAiHPA037508.
HPA043308.

Interactioni

Subunit structurei

Interacts with p300/EP300, the complex being recruited to activated p53/TP53. Interacts with TTC5 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CCDC136Q96JN2-23EBI-10268138,EBI-10171416
MDM2Q009872EBI-866435,EBI-389668

Protein-protein interaction databases

BioGridi126372. 7 interactors.
IntActiQ8N9B5. 8 interactors.
MINTiMINT-4052528.
STRINGi9606.ENSP00000379441.

Structurei

3D structure databases

ProteinModelPortaliQ8N9B5.
SMRiQ8N9B5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini921 – 938WH2PROSITE-ProRule annotationAdd BLAST18

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 126Interaction with p300/EP300By similarityAdd BLAST126
Regioni478 – 567Interaction with p300/EP300By similarityAdd BLAST90

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili324 – 360Sequence analysisAdd BLAST37
Coiled coili489 – 541Sequence analysisAdd BLAST53
Coiled coili590 – 621Sequence analysisAdd BLAST32

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi781 – 827Pro-richAdd BLAST47

Sequence similaritiesi

Belongs to the JMY family.Curated
Contains 1 WH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IF4S. Eukaryota.
ENOG4111J4B. LUCA.
GeneTreeiENSGT00510000046704.
HOGENOMiHOG000074181.
HOVERGENiHBG067146.
InParanoidiQ8N9B5.
OMAiEDQCDSL.
OrthoDBiEOG091G0217.
PhylomeDBiQ8N9B5.
TreeFamiTF331023.

Family and domain databases

InterProiIPR033324. Jmy.
IPR031738. JMY/WHAMM.
IPR031808. JMY/WHAMM_N.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23330:SF8. PTHR23330:SF8. 2 hits.
PfamiPF15871. JMY. 1 hit.
PF15920. WHAMM-JMY_N. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N9B5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFALEETLE SDWVAVRPHV FDEREKHKFV FIVAWNEIEG KFAITCHNRT
60 70 80 90 100
AQRQRSGSRE QAGARGGAEA GGAASDGSRG PGSPAGRGRP EATASATLVR
110 120 130 140 150
SPGPRRSSAW AEGGSPRSTR SLLGDPRLRS PGSKGAESRL RSPVRAKPIP
160 170 180 190 200
GQKTSEADDA AGAAAAAARP APREAQVSSV RIVSASGTVS EEIEVLEMVK
210 220 230 240 250
EDEAPLALSD AEQPPPATEL ESPAEECSWA GLFSFQDLRA VHQQLCSVNS
260 270 280 290 300
QLEPCLPVFP EEPSGMWTVL FGGAPEMTEQ EIDTLCYQLQ VYLGHGLDTC
310 320 330 340 350
GWKILSQVLF TETDDPEEYY ESLSELRQKG YEEVLQRARK RIQELLDKHK
360 370 380 390 400
NTESMVELLD LYQMEDEAYS SLAEATTELY QYLLQPFRDM RELAMLRRQQ
410 420 430 440 450
IKISMENDYL GPRRIESLQK EDADWQRKAH MAVLSIQDLT VKYFEITAKA
460 470 480 490 500
QKAVYDRMRA DQKKFGKASW AAAAERMEKL QYAVSKETLQ MMRAKEICLE
510 520 530 540 550
QRKHALKEEM QSLRGGTEAI ARLDQLEADY YDLQLQLYEV QFEILKCEEL
560 570 580 590 600
LLTAQLESIK RLISEKRDEV VYYDTYESME AMLEKEEMAA SAYLQREELQ
610 620 630 640 650
KLQQKARQLE ARRGRVSAKK SYLRNKKEIC IAKHNEKIQQ RTRIEDEYRT
660 670 680 690 700
HHTVQLKREK LHDEEERKSA WVSQERQRTL DRLRTFKQRY PGQVILKSTR
710 720 730 740 750
LRLAHARRKG AASPVLQEDH CDSLPSVLQV EEKTEEVGEG RVKRGPSQTT
760 770 780 790 800
EPQSLVQLED TSLTQLEATS LPLSGVTSEL PPTISLPLLN NNLEPCSVTI
810 820 830 840 850
NPLPSPLPPT PPPPPPPPPP PPPPPLPVAK DSGPETLEKD LPRKEGNEKR
860 870 880 890 900
IPKSASAPSA HLFDSSQLVS ARKKLRKTAE GLQRRRVSSP MDEVLASLKR
910 920 930 940 950
GSFHLKKVEQ RTLPPFPDED DSNNILAQIR KGVKLKKVQK DVLRESFTLL
960 970 980
PDTDPLTRSI HEALRRIKEA SPESEDEEEA LPCTDWEN
Length:988
Mass (Da):111,445
Last modified:March 18, 2008 - v2
Checksum:i7166FF8AE325F37A
GO
Isoform 2 (identifier: Q8N9B5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     814-825: Missing.

Show »
Length:976
Mass (Da):110,280
Checksum:i27175E3EFA32295E
GO

Sequence cautioni

The sequence AAI30625 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC04495 differs from that shown. Reason: Erroneous initiation.Curated
The sequence EAW95834 differs from that shown. Reason: Erroneous gene model prediction.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_039846364M → L.2 PublicationsCorresponds to variant rs13182512dbSNPEnsembl.1
Natural variantiVAR_039847592A → V.Corresponds to variant rs12109475dbSNPEnsembl.1
Natural variantiVAR_039848720H → R.Corresponds to variant rs16876657dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_032310814 – 825Missing in isoform 2. 1 PublicationAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC016559 Genomic DNA. No translation available.
AC020898 Genomic DNA. No translation available.
CH471084 Genomic DNA. Translation: EAW95834.1. Sequence problems.
BC130624 mRNA. Translation: AAI30625.1. Different initiation.
AK095189 mRNA. Translation: BAC04495.1. Different initiation.
CCDSiCCDS4047.3. [Q8N9B5-1]
RefSeqiNP_689618.4. NM_152405.4. [Q8N9B5-1]
UniGeneiHs.482605.

Genome annotation databases

EnsembliENST00000396137; ENSP00000379441; ENSG00000152409. [Q8N9B5-1]
GeneIDi133746.
KEGGihsa:133746.
UCSCiuc003kfx.5. human. [Q8N9B5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC016559 Genomic DNA. No translation available.
AC020898 Genomic DNA. No translation available.
CH471084 Genomic DNA. Translation: EAW95834.1. Sequence problems.
BC130624 mRNA. Translation: AAI30625.1. Different initiation.
AK095189 mRNA. Translation: BAC04495.1. Different initiation.
CCDSiCCDS4047.3. [Q8N9B5-1]
RefSeqiNP_689618.4. NM_152405.4. [Q8N9B5-1]
UniGeneiHs.482605.

3D structure databases

ProteinModelPortaliQ8N9B5.
SMRiQ8N9B5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126372. 7 interactors.
IntActiQ8N9B5. 8 interactors.
MINTiMINT-4052528.
STRINGi9606.ENSP00000379441.

PTM databases

iPTMnetiQ8N9B5.
PhosphoSitePlusiQ8N9B5.

Polymorphism and mutation databases

BioMutaiJMY.
DMDMi172045777.

Proteomic databases

EPDiQ8N9B5.
MaxQBiQ8N9B5.
PaxDbiQ8N9B5.
PeptideAtlasiQ8N9B5.
PRIDEiQ8N9B5.

Protocols and materials databases

DNASUi133746.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000396137; ENSP00000379441; ENSG00000152409. [Q8N9B5-1]
GeneIDi133746.
KEGGihsa:133746.
UCSCiuc003kfx.5. human. [Q8N9B5-1]

Organism-specific databases

CTDi133746.
DisGeNETi133746.
GeneCardsiJMY.
H-InvDBHIX0004982.
HGNCiHGNC:28916. JMY.
HPAiHPA037508.
HPA043308.
MIMi604279. gene.
neXtProtiNX_Q8N9B5.
OpenTargetsiENSG00000152409.
PharmGKBiPA164721124.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IF4S. Eukaryota.
ENOG4111J4B. LUCA.
GeneTreeiENSGT00510000046704.
HOGENOMiHOG000074181.
HOVERGENiHBG067146.
InParanoidiQ8N9B5.
OMAiEDQCDSL.
OrthoDBiEOG091G0217.
PhylomeDBiQ8N9B5.
TreeFamiTF331023.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000152409-MONOMER.
ReactomeiR-HSA-6804760. Regulation of TP53 Activity through Methylation.

Miscellaneous databases

GenomeRNAii133746.
PMAP-CutDBQ8N9B5.
PROiQ8N9B5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000152409.
GenevisibleiQ8N9B5. HS.

Family and domain databases

InterProiIPR033324. Jmy.
IPR031738. JMY/WHAMM.
IPR031808. JMY/WHAMM_N.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23330:SF8. PTHR23330:SF8. 2 hits.
PfamiPF15871. JMY. 1 hit.
PF15920. WHAMM-JMY_N. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiJMY_HUMAN
AccessioniPrimary (citable) accession number: Q8N9B5
Secondary accession number(s): A1L4P5, B5MDS2, B5MDT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: November 30, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.