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Q8N9A8 (NEPR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear envelope phosphatase-regulatory subunit 1

Short name=NEP1-R1
Alternative name(s):
Transmembrane protein 188
Gene names
Name:CNEP1R1
Synonyms:C16orf69, TMEM188
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length125 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Forms with the serine/threonine protein phosphatase CTDNEP1 an active complex which dephosphorylates and may activate LPIN1 and LPIN2. LPIN1 and LPIN2 are phosphatidate phosphatases that catalyze the conversion of phosphatidic acid to diacylglycerol and control the metabolism of fatty acids at differents levels. May indirectly modulate the lipid composition of nuclear and/or endoplasmic reticulum membranes and be required for proper nuclear membrane morphology and/or dynamics. May also indirectly regulate the production of lipid droplets and triacylglycerol. Ref.5

Subunit structure

Interacts with CTDNEP1; the complex dephosphorylates LPIN1 and LPIN2. Ref.5

Subcellular location

Nucleus membrane; Multi-pass membrane protein. Cytoplasm. Note: Filamentous pattern in the cytoplasm. Ref.5

Tissue specificity

Muscle specific with lower expression in other metabolic tissues. Ref.5

Sequence similarities

Belongs to the CNEP1R1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CTDNEP1O954764EBI-5323455,EBI-5323433

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8N9A8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8N9A8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     8-8: E → EAPRVVSLIPAVVSGNCQ
Isoform 3 (identifier: Q8N9A8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     33-47: MLLIVVSVCTATGAW → SVLLHIIMESPIFHH
     48-125: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 125125Nuclear envelope phosphatase-regulatory subunit 1
PRO_0000286615

Regions

Transmembrane33 – 5321Helical; Potential
Transmembrane65 – 8521Helical; Potential

Amino acid modifications

Modified residue11N-acetylmethionine Ref.6

Natural variations

Alternative sequence81E → EAPRVVSLIPAVVSGNCQ in isoform 2.
VSP_025126
Alternative sequence33 – 4715MLLIV…ATGAW → SVLLHIIMESPIFHH in isoform 3.
VSP_025127
Alternative sequence48 – 12578Missing in isoform 3.
VSP_025128

Experimental info

Sequence conflict151R → G in CAI45924. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 546EB9BEFE8EA593

FASTA12514,267
        10         20         30         40         50         60 
MNSLEQAEDL KAFERRLTEY IHCLQPATGR WRMLLIVVSV CTATGAWNWL IDPETQKVSF 

        70         80         90        100        110        120 
FTSLWNHPFF TISCITLIGL FFAGIHKRVV APSIIAARCR TVLAEYNMSC DDTGKLILKP 


RPHVQ 

« Hide

Isoform 2 [UniParc].

Checksum: 3BA907B8B39FADE6
Show »

FASTA14215,959
Isoform 3 [UniParc].

Checksum: 1FFD52570FA8A391
Show »

FASTA475,618

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Adipose tissue.
[3]"The sequence and analysis of duplication-rich human chromosome 16."
Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. expand/collapse author list , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Brain and Testis.
[5]"Nuclear envelope phosphatase-regulatory subunit 1 (formerly TMEM188) is the metazoan SPO7 ortholog and functions in the lipin activation pathway."
Han S., Bahmanyar S., Zhang P., Grishin N., Oegema K., Crooke R., Graham M., Reue K., Dixon J.E., Goodman J.M.
J. Biol. Chem. 287:3123-3137(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN LPIN1 AND LPIN2 DEPHOSPHORYLATION, INTERACTION WITH CTDNEP1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK095420 mRNA. Translation: BAC04545.1.
CR933600 mRNA. Translation: CAI45924.1.
AC007610 Genomic DNA. No translation available.
BC022550 mRNA. Translation: AAH22550.1.
BC036683 mRNA. Translation: AAH36683.1.
CCDSCCDS45480.1. [Q8N9A8-2]
CCDS61931.1. [Q8N9A8-1]
RefSeqNP_001268718.1. NM_001281789.1. [Q8N9A8-1]
NP_694993.2. NM_153261.5. [Q8N9A8-2]
UniGeneHs.59134.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid129126. 2 interactions.
IntActQ8N9A8. 1 interaction.
STRING9606.ENSP00000405635.

Polymorphism databases

DMDM74729639.

Proteomic databases

MaxQBQ8N9A8.
PRIDEQ8N9A8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000427478; ENSP00000394224; ENSG00000205423. [Q8N9A8-1]
ENST00000458059; ENSP00000405635; ENSG00000205423. [Q8N9A8-2]
ENST00000565457; ENSP00000456686; ENSG00000205423. [Q8N9A8-1]
ENST00000566482; ENSP00000456294; ENSG00000205423. [Q8N9A8-3]
ENST00000568890; ENSP00000456913; ENSG00000205423. [Q8N9A8-3]
GeneID255919.
KEGGhsa:255919.
UCSCuc002eft.3. human. [Q8N9A8-2]
uc002efu.3. human. [Q8N9A8-1]

Organism-specific databases

CTD255919.
GeneCardsGC16P050058.
H-InvDBHIX0013019.
HGNCHGNC:26759. CNEP1R1.
HPAHPA042815.
neXtProtNX_Q8N9A8.
PharmGKBPA162406208.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG241470.
HOVERGENHBG107287.
OMACEDLKAF.
OrthoDBEOG7XSTGM.
PhylomeDBQ8N9A8.
TreeFamTF313179.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.
REACT_21300. Mitotic M-M/G1 phases.

Gene expression databases

ArrayExpressQ8N9A8.
BgeeQ8N9A8.
CleanExHS_TMEM188.
GenevestigatorQ8N9A8.

Family and domain databases

InterProIPR019168. NEP1-R1.
[Graphical view]
PANTHERPTHR20996. PTHR20996. 1 hit.
PfamPF09771. Tmemb_18A. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi255919.
NextBio92676.
PROQ8N9A8.

Entry information

Entry nameNEPR1_HUMAN
AccessionPrimary (citable) accession number: Q8N9A8
Secondary accession number(s): Q4G1A9, Q5H9V0, Q8NE06
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

SIMILARITY comments

Index of protein domains and families

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM