Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

N-terminal EF-hand calcium-binding protein 1

Gene

NECAB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi39 – 50121PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal EF-hand calcium-binding protein 1
Short name:
EF-hand calcium-binding protein 1
Alternative name(s):
Neuronal calcium-binding protein 1
Gene namesi
Name:NECAB1
Synonyms:EFCBP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:20983. NECAB1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162397411.

Polymorphism and mutation databases

BioMutaiNECAB1.
DMDMi74760025.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351N-terminal EF-hand calcium-binding protein 1PRO_0000282609Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41PhosphoserineBy similarity
Modified residuei192 – 1921PhosphoserineBy similarity
Modified residuei197 – 1971PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8N987.
PeptideAtlasiQ8N987.
PRIDEiQ8N987.

PTM databases

iPTMnetiQ8N987.
PhosphoSiteiQ8N987.

Expressioni

Tissue specificityi

Expressed in brain (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000123119.
CleanExiHS_NECAB1.
GenevisibleiQ8N987. HS.

Organism-specific databases

HPAiCAB068208.
CAB068209.
CAB068210.
HPA023252.
HPA023629.
HPA025963.
HPA031262.

Interactioni

Subunit structurei

Interacts with STX1 (PubMed:12044471). May interact with CPNE6 (PubMed:12044471).1 Publication

Protein-protein interaction databases

BioGridi122093. 11 interactions.
STRINGi9606.ENSP00000387380.

Structurei

3D structure databases

ProteinModelPortaliQ8N987.
SMRiQ8N987. Positions 31-90.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 6136EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini60 – 9536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini252 – 34089ABMAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili135 – 16329Sequence analysisAdd
BLAST
Coiled coili209 – 27567Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 ABM domain.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IH3U. Eukaryota.
ENOG4111GJU. LUCA.
GeneTreeiENSGT00390000009734.
HOGENOMiHOG000008120.
HOVERGENiHBG050433.
InParanoidiQ8N987.
OMAiSQWMTQI.
OrthoDBiEOG091G0DWL.
PhylomeDBiQ8N987.
TreeFamiTF331029.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR007138. ABM_dom.
IPR011008. Dimeric_a/b-barrel.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF03992. ABM. 1 hit.
PF13202. EF-hand_5. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF54909. SSF54909. 1 hit.
PROSITEiPS51725. ABM. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N987-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDSQETSPS SNNSSEELSS ALHLSKGMSI FLDILRRADK NDDGKLSFEE
60 70 80 90 100
FKAYFADGVL SGEELHELFH TIDTHNTNNL DTEELCEYFS QHLGEYENVL
110 120 130 140 150
AALEDLNLSI LKAMGKTKKD YQEASNLEQF VTRFLLKETL NQLQSLQNSL
160 170 180 190 200
ECAMETTEEQ TRQERQGPAK PEVLSIQWPG KRSSRRVQRH NSFSPNSPQF
210 220 230 240 250
NVSGPGLLEE DNQWMTQINR LQKLIDRLEK KDLKLEPPEE EIIEGNTKSH
260 270 280 290 300
IMLVQRQMSV IEEDLEEFQL ALKHYVESAS SQSGCLRISI QKLSNESRYM
310 320 330 340 350
IYEFWENSSV WNSHLQTNYS KTFQRSNVDF LETPELTSTM LVPASWWILN

N
Length:351
Mass (Da):40,571
Last modified:October 1, 2002 - v1
Checksum:i74D5E5895B4FECFA
GO
Isoform 2 (identifier: Q8N987-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-251: Missing.

Show »
Length:100
Mass (Da):11,814
Checksum:iC9B90480302ED8CC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1051D → G in AAH68448 (PubMed:15489334).Curated
Sequence conflicti261 – 2611I → M in AAH16340 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 251251Missing in isoform 2. 1 PublicationVSP_024214Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY224210 mRNA. Translation: AAO34126.1.
AF414126 mRNA. Translation: AAP33146.1.
AK095533 mRNA. Translation: BAC04568.1.
BC016340 mRNA. Translation: AAH16340.1.
BC068448 mRNA. Translation: AAH68448.1.
AF193756 mRNA. Translation: AAG28412.1.
CCDSiCCDS47889.1. [Q8N987-1]
RefSeqiNP_071746.1. NM_022351.4. [Q8N987-1]
UniGeneiHs.719466.

Genome annotation databases

EnsembliENST00000417640; ENSP00000387380; ENSG00000123119. [Q8N987-1]
ENST00000521366; ENSP00000428632; ENSG00000123119. [Q8N987-2]
ENST00000522820; ENSP00000428953; ENSG00000123119. [Q8N987-2]
GeneIDi64168.
KEGGihsa:64168.
UCSCiuc003yer.4. human. [Q8N987-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY224210 mRNA. Translation: AAO34126.1.
AF414126 mRNA. Translation: AAP33146.1.
AK095533 mRNA. Translation: BAC04568.1.
BC016340 mRNA. Translation: AAH16340.1.
BC068448 mRNA. Translation: AAH68448.1.
AF193756 mRNA. Translation: AAG28412.1.
CCDSiCCDS47889.1. [Q8N987-1]
RefSeqiNP_071746.1. NM_022351.4. [Q8N987-1]
UniGeneiHs.719466.

3D structure databases

ProteinModelPortaliQ8N987.
SMRiQ8N987. Positions 31-90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122093. 11 interactions.
STRINGi9606.ENSP00000387380.

PTM databases

iPTMnetiQ8N987.
PhosphoSiteiQ8N987.

Polymorphism and mutation databases

BioMutaiNECAB1.
DMDMi74760025.

Proteomic databases

PaxDbiQ8N987.
PeptideAtlasiQ8N987.
PRIDEiQ8N987.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000417640; ENSP00000387380; ENSG00000123119. [Q8N987-1]
ENST00000521366; ENSP00000428632; ENSG00000123119. [Q8N987-2]
ENST00000522820; ENSP00000428953; ENSG00000123119. [Q8N987-2]
GeneIDi64168.
KEGGihsa:64168.
UCSCiuc003yer.4. human. [Q8N987-1]

Organism-specific databases

CTDi64168.
GeneCardsiNECAB1.
HGNCiHGNC:20983. NECAB1.
HPAiCAB068208.
CAB068209.
CAB068210.
HPA023252.
HPA023629.
HPA025963.
HPA031262.
neXtProtiNX_Q8N987.
PharmGKBiPA162397411.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IH3U. Eukaryota.
ENOG4111GJU. LUCA.
GeneTreeiENSGT00390000009734.
HOGENOMiHOG000008120.
HOVERGENiHBG050433.
InParanoidiQ8N987.
OMAiSQWMTQI.
OrthoDBiEOG091G0DWL.
PhylomeDBiQ8N987.
TreeFamiTF331029.

Miscellaneous databases

GenomeRNAii64168.
PROiQ8N987.

Gene expression databases

BgeeiENSG00000123119.
CleanExiHS_NECAB1.
GenevisibleiQ8N987. HS.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR007138. ABM_dom.
IPR011008. Dimeric_a/b-barrel.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF03992. ABM. 1 hit.
PF13202. EF-hand_5. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF54909. SSF54909. 1 hit.
PROSITEiPS51725. ABM. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNECA1_HUMAN
AccessioniPrimary (citable) accession number: Q8N987
Secondary accession number(s): Q6NUS7, Q96AZ7, Q9HBW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.