Reviewed, UniProtKB/Swiss-Prot Q8N983 (RM43_HUMAN)

Names and origin

Protein names

Recommended name:
    39S ribosomal protein L43, mitochondrial
      Short name=L43mt
      Short name=MRP-L43
Alternative name(s):
    Mitochondrial ribosomal protein bMRP36a

Gene names

Name:

MRPL43

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Mitochondrion. Ref.1
Tissue specificity	High relative levels in skeletal muscle and testis. Lower levels of expression in the heart, brain, placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and leukocytes. Expression is coregulated with PEO1. Ref.5
Sequence similarities	Belongs to the ribosomal protein S25/L51 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Gene Ontology (GO)
Biological process	translation Non-traceable author statement. Source: UniProtKB
Cellular component	mitochondrial ribosome Non-traceable author statement. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural constituent of ribosome Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARRB2	P32121	1	EBI-1043145,EBI-714559
DDX56	Q9NY93	1	EBI-1043145,EBI-372376

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N983-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N983-2)

The sequence of this isoform differs from the canonical sequence as follows:
     177-215: PDLPTVDPISSSLTSAPAPMLSAVSCLPIVPALTTVCSA → ISVQSSDLPWGNTHYRPEPLSSTTWL

Isoform 3 (identifier: Q8N983-3)

The sequence of this isoform differs from the canonical sequence as follows:
     156-164: DTGLRLSAV → CLLLGAVTL
     165-215: Missing.

Isoform 4 (identifier: Q8N983-4)

The sequence of this isoform differs from the canonical sequence as follows:
     156-159: DTGL → VQAQ
     160-215: Missing.

Note: Ref.1 (BAB40861) sequence differs from that shown due to frameshifts in positions 131 and 210.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 215		39S ribosomal protein L43, mitochondrial		PRO_0000030561
Natural variations
	Alternative sequence	156 – 164	9	DTGLRLSAV → CLLLGAVTL in isoform 3.		VSP_011027
	Alternative sequence	156 – 159	4	DTGL → VQAQ in isoform 4.		VSP_011029
	Alternative sequence	160 – 215	56	Missing in isoform 4.		VSP_011030
	Alternative sequence	165 – 215	51	Missing in isoform 3.		VSP_011028
	Alternative sequence	177 – 215	39	PDLPT…TVCSA → ISVQSSDLPWGNTHYRPEPL SSTTWL in isoform 2.		VSP_011026

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 059C6E36FEB0D235	FASTA	215	23,431
	10 20 30 40 50 60 MTARGTPSRF LASVLHNGLG RYVQQLQRLS FSVSRDGASS RGAREFVERE VIDFARRNPG 70 80 90 100 110 120 VVIYVNSRPC CVPRVVAEYL NGAVREESIH CKSVEEISTL VQKLADQSGL DVIRIRKPFH 130 140 150 160 170 180 TDNPSIQGQW HPFTNKPTTF RGLRPREVQD PAPAQDTGLR LSAVAPQILL PGWPDPPDLP 190 200 210 TVDPISSSLT SAPAPMLSAV SCLPIVPALT TVCSA
	Isoform 2. Checksum: 30A1ACC6D3F30CA8	FASTA	202	22,580
	10 20 30 40 50 60 MTARGTPSRF LASVLHNGLG RYVQQLQRLS FSVSRDGASS RGAREFVERE VIDFARRNPG 70 80 90 100 110 120 VVIYVNSRPC CVPRVVAEYL NGAVREESIH CKSVEEISTL VQKLADQSGL DVIRIRKPFH 130 140 150 160 170 180 TDNPSIQGQW HPFTNKPTTF RGLRPREVQD PAPAQDTGLR LSAVAPQILL PGWPDPISVQ 190 200 SSDLPWGNTH YRPEPLSSTT WL
	Isoform 3. Checksum: 8BBA76364E45CA58	FASTA	164	18,311
	10 20 30 40 50 60 MTARGTPSRF LASVLHNGLG RYVQQLQRLS FSVSRDGASS RGAREFVERE VIDFARRNPG 70 80 90 100 110 120 VVIYVNSRPC CVPRVVAEYL NGAVREESIH CKSVEEISTL VQKLADQSGL DVIRIRKPFH 130 140 150 160 TDNPSIQGQW HPFTNKPTTF RGLRPREVQD PAPAQCLLLG AVTL
	Isoform 4. Checksum: 3430B94F1152F375	FASTA	159	17,853
	10 20 30 40 50 60 MTARGTPSRF LASVLHNGLG RYVQQLQRLS FSVSRDGASS RGAREFVERE VIDFARRNPG 70 80 90 100 110 120 VVIYVNSRPC CVPRVVAEYL NGAVREESIH CKSVEEISTL VQKLADQSGL DVIRIRKPFH 130 140 150 TDNPSIQGQW HPFTNKPTTF RGLRPREVQD PAPAQVQAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural compensation for the deficit of rRNA with proteins in the mammalian mitochondrial ribosome. Systematic analysis of protein components of the large ribosomal subunit from mammalian mitochondria." Suzuki T., Terasaki M., Takemoto-Hori C., Hanada T., Ueda T., Wada A., Watanabe K. J. Biol. Chem. 276:21724-21736(2001) [PubMed: 11279069] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), SUBCELLULAR LOCATION.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4). Tissue: Ovary, Pancreas, Skin and Uterus.
[5]	"Twinkle helicase is essential for mtDNA maintenance and regulates mtDNA copy number." Tyynismaa H., Sembongi H., Bokori-Brown M., Granycome C., Ashley N., Poulton J., Jalanko A., Spelbrink J.N., Holt I.J., Suomalainen A. Hum. Mol. Genet. 13:3219-3227(2004) [PubMed: 15509589] [Abstract] Cited for: TISSUE SPECIFICITY, COREGULATION WITH PEO1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB049656 mRNA. Translation: BAB40861.1. Frameshift. AK095556 mRNA. Translation: BAC04572.1. AL133215 Genomic DNA. Translation: CAI10919.1. AL133215 Genomic DNA. Translation: CAI10922.1. BC015905 mRNA. Translation: AAH15905.1. BC041165 mRNA. Translation: AAH41165.1. BC052639 mRNA. Translation: AAH52639.1. BC053373 mRNA. Translation: AAH53373.1.
IPI	IPI00428285. IPI00428286. IPI00428287. IPI00428288.
RefSeq	NP_789762.1. NP_789763.1.
UniGene	Hs.421848
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N983. 2 interactions.
Proteomic databases
PRIDE	Q8N983.
Genome annotation databases
Ensembl	ENSG00000055950. Homo sapiens. [Contig view]
GeneID	84545.
Organism-specific databases
GeneCards	GC10M102727.
H-InvDB	HIX0009131.
HGNC	HGNC:14517. MRPL43.
MIM	611848. gene.
PharmGKB	PA30975.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q8N983.
OMA	Q8N983. WGNTHYR.
Gene expression databases
ArrayExpress	Q8N983.
Bgee	Q8N983.
CleanEx	HS_MRPL43.
GermOnline	ENSG00000055950. Homo sapiens.
Family and domain databases
InterPro	IPR007741. Ribosome/NADH_DH. [Graphical view]
Pfam	PF05047. L51_S25_CI-B8. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	74406.
SOURCE	Search...

Entry information

Entry name

RM43_HUMAN

Accession

Primary (citable) accession number: Q8N983
Secondary accession number(s): B1AL09 , Q5JW06, Q7Z719, Q7Z7H6, Q86XN1, Q9BYC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 1, 2002
Last modified:	May 5, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)