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Protein

Prostaglandin reductase 2

Gene

PTGR2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation (By similarity).By similarity1 Publication

Catalytic activityi

11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.1 Publication

Kineticsi

  1. KM=11.21 µM for 15-keto-PGE21 Publication
  2. KM=15.87 µM for NADPH1 Publication
  1. Vmax=159 nmol/min/mg enzyme for 15-keto-PGE21 Publication
  2. Vmax=67 nmol/min/mg enzyme for NADPH1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei192NADP1
Binding sitei208NADP1
Binding sitei231NADP1
Binding sitei337NADP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 168NADP4
Nucleotide bindingi253 – 259NADP7
Nucleotide bindingi287 – 289NADP3

GO - Molecular functioni

  • 13-prostaglandin reductase activity Source: UniProtKB-EC
  • 15-oxoprostaglandin 13-oxidase activity Source: UniProtKB

GO - Biological processi

  • prostaglandin metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciZFISH:HS06681-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin reductase 2 (EC:1.3.1.48)
Short name:
PRG-2
Alternative name(s):
15-oxoprostaglandin 13-reductase
Zinc-binding alcohol dehydrogenase domain-containing protein 1
Gene namesi
Name:PTGR2
Synonyms:ZADH1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:20149. PTGR2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi64Y → F: Increases affinity for 15-keto-PGE2. Reduces affinity for NADP and Vmax. 1 Publication1
Mutagenesisi259Y → F: Increases affinity for 15-keto-PGE2. Reduces affinity for NADP and Vmax. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000140043.
PharmGKBiPA162400323.

Chemistry databases

DrugBankiDB00328. Indomethacin.

Polymorphism and mutation databases

BioMutaiPTGR2.
DMDMi62901454.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002180701 – 351Prostaglandin reductase 2Add BLAST351

Proteomic databases

EPDiQ8N8N7.
PaxDbiQ8N8N7.
PeptideAtlasiQ8N8N7.
PRIDEiQ8N8N7.

2D gel databases

REPRODUCTION-2DPAGEIPI00167515.

PTM databases

iPTMnetiQ8N8N7.
PhosphoSitePlusiQ8N8N7.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000140043.
CleanExiHS_PTGR2.
ExpressionAtlasiQ8N8N7. baseline and differential.
GenevisibleiQ8N8N7. HS.

Organism-specific databases

HPAiHPA001125.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi126915. 15 interactors.
STRINGi9606.ENSP00000267568.

Structurei

Secondary structure

1351
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Helixi20 – 22Combined sources3
Beta strandi23 – 29Combined sources7
Beta strandi38 – 47Combined sources10
Helixi52 – 55Combined sources4
Beta strandi57 – 59Combined sources3
Beta strandi61 – 65Combined sources5
Beta strandi75 – 85Combined sources11
Beta strandi95 – 110Combined sources16
Helixi111 – 113Combined sources3
Beta strandi115 – 117Combined sources3
Helixi119 – 122Combined sources4
Helixi126 – 130Combined sources5
Turni131 – 133Combined sources3
Helixi135 – 147Combined sources13
Beta strandi157 – 162Combined sources6
Helixi166 – 177Combined sources12
Beta strandi181 – 188Combined sources8
Helixi190 – 198Combined sources9
Beta strandi203 – 207Combined sources5
Turni208 – 210Combined sources3
Helixi213 – 220Combined sources8
Beta strandi225 – 231Combined sources7
Helixi234 – 242Combined sources9
Beta strandi244 – 252Combined sources9
Helixi256 – 258Combined sources3
Helixi271 – 280Combined sources10
Beta strandi283 – 286Combined sources4
Helixi289 – 295Combined sources7
Helixi296 – 308Combined sources13
Beta strandi316 – 320Combined sources5
Helixi322 – 324Combined sources3
Helixi325 – 333Combined sources9
Beta strandi338 – 344Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VNAX-ray2.17A1-349[»]
2W4QX-ray2.00A1-349[»]
2W98X-ray1.85A/B1-349[»]
2ZB4X-ray1.63A1-351[»]
2ZB7X-ray1.80A1-351[»]
2ZB8X-ray2.00A1-351[»]
ProteinModelPortaliQ8N8N7.
SMRiQ8N8N7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8N8N7.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni99 – 100Substrate binding2
Regioni288 – 290Substrate binding3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1196. Eukaryota.
COG2130. LUCA.
GeneTreeiENSGT00390000009335.
HOGENOMiHOG000294663.
HOVERGENiHBG055024.
InParanoidiQ8N8N7.
KOiK13949.
OMAiVNGLENM.
OrthoDBiEOG091G0F5B.
PhylomeDBiQ8N8N7.
TreeFamiTF324201.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N8N7-1) [UniParc]FASTAAdd to basket
Also known as: ZADH1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIVQRVVLNS RPGKNGNPVA ENFRMEEVYL PDNINEGQVQ VRTLYLSVDP
60 70 80 90 100
YMRCRMNEDT GTDYITPWQL SQVVDGGGIG IIEESKHTNL TKGDFVTSFY
110 120 130 140 150
WPWQTKVILD GNSLEKVDPQ LVDGHLSYFL GAIGMPGLTS LIGIQEKGHI
160 170 180 190 200
TAGSNKTMVV SGAAGACGSV AGQIGHFLGC SRVVGICGTH EKCILLTSEL
210 220 230 240 250
GFDAAINYKK DNVAEQLRES CPAGVDVYFD NVGGNISDTV ISQMNENSHI
260 270 280 290 300
ILCGQISQYN KDVPYPPPLS PAIEAIQKER NITRERFLVL NYKDKFEPGI
310 320 330 340 350
LQLSQWFKEG KLKIKETVIN GLENMGAAFQ SMMTGGNIGK QIVCISEEIS

L
Length:351
Mass (Da):38,499
Last modified:October 1, 2002 - v1
Checksum:i118ED6D2FB984F3A
GO
Isoform 2 (identifier: Q8N8N7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     174-180: IGHFLGC → VNFLRII
     181-351: Missing.

Note: No experimental confirmation available.
Show »
Length:180
Mass (Da):19,675
Checksum:iB2E6EB6A71991004
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013526174 – 180IGHFLGC → VNFLRII in isoform 2. 1 Publication7
Alternative sequenceiVSP_013527181 – 351Missing in isoform 2. 1 PublicationAdd BLAST171

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY346133 mRNA. Translation: AAR05101.1.
AY424308 mRNA. Translation: AAR82927.1.
AK096410 mRNA. Translation: BAC04781.1.
BX641118 mRNA. Translation: CAE46055.1.
CH471061 Genomic DNA. Translation: EAW81132.1.
BC059364 mRNA. Translation: AAH59364.1.
CCDSiCCDS9820.1. [Q8N8N7-1]
RefSeqiNP_001139626.1. NM_001146154.1. [Q8N8N7-1]
NP_001139627.1. NM_001146155.1. [Q8N8N7-1]
NP_689657.1. NM_152444.2. [Q8N8N7-1]
UniGeneiHs.632344.

Genome annotation databases

EnsembliENST00000267568; ENSP00000267568; ENSG00000140043. [Q8N8N7-1]
ENST00000555228; ENSP00000450975; ENSG00000140043. [Q8N8N7-1]
ENST00000555661; ENSP00000452280; ENSG00000140043. [Q8N8N7-1]
GeneIDi145482.
KEGGihsa:145482.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY346133 mRNA. Translation: AAR05101.1.
AY424308 mRNA. Translation: AAR82927.1.
AK096410 mRNA. Translation: BAC04781.1.
BX641118 mRNA. Translation: CAE46055.1.
CH471061 Genomic DNA. Translation: EAW81132.1.
BC059364 mRNA. Translation: AAH59364.1.
CCDSiCCDS9820.1. [Q8N8N7-1]
RefSeqiNP_001139626.1. NM_001146154.1. [Q8N8N7-1]
NP_001139627.1. NM_001146155.1. [Q8N8N7-1]
NP_689657.1. NM_152444.2. [Q8N8N7-1]
UniGeneiHs.632344.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VNAX-ray2.17A1-349[»]
2W4QX-ray2.00A1-349[»]
2W98X-ray1.85A/B1-349[»]
2ZB4X-ray1.63A1-351[»]
2ZB7X-ray1.80A1-351[»]
2ZB8X-ray2.00A1-351[»]
ProteinModelPortaliQ8N8N7.
SMRiQ8N8N7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126915. 15 interactors.
STRINGi9606.ENSP00000267568.

Chemistry databases

DrugBankiDB00328. Indomethacin.

PTM databases

iPTMnetiQ8N8N7.
PhosphoSitePlusiQ8N8N7.

Polymorphism and mutation databases

BioMutaiPTGR2.
DMDMi62901454.

2D gel databases

REPRODUCTION-2DPAGEIPI00167515.

Proteomic databases

EPDiQ8N8N7.
PaxDbiQ8N8N7.
PeptideAtlasiQ8N8N7.
PRIDEiQ8N8N7.

Protocols and materials databases

DNASUi145482.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000267568; ENSP00000267568; ENSG00000140043. [Q8N8N7-1]
ENST00000555228; ENSP00000450975; ENSG00000140043. [Q8N8N7-1]
ENST00000555661; ENSP00000452280; ENSG00000140043. [Q8N8N7-1]
GeneIDi145482.
KEGGihsa:145482.

Organism-specific databases

CTDi145482.
GeneCardsiPTGR2.
HGNCiHGNC:20149. PTGR2.
HPAiHPA001125.
MIMi608642. gene.
neXtProtiNX_Q8N8N7.
OpenTargetsiENSG00000140043.
PharmGKBiPA162400323.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1196. Eukaryota.
COG2130. LUCA.
GeneTreeiENSGT00390000009335.
HOGENOMiHOG000294663.
HOVERGENiHBG055024.
InParanoidiQ8N8N7.
KOiK13949.
OMAiVNGLENM.
OrthoDBiEOG091G0F5B.
PhylomeDBiQ8N8N7.
TreeFamiTF324201.

Enzyme and pathway databases

BioCyciZFISH:HS06681-MONOMER.

Miscellaneous databases

ChiTaRSiPTGR2. human.
EvolutionaryTraceiQ8N8N7.
GenomeRNAii145482.
PROiQ8N8N7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000140043.
CleanExiHS_PTGR2.
ExpressionAtlasiQ8N8N7. baseline and differential.
GenevisibleiQ8N8N7. HS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPTGR2_HUMAN
AccessioniPrimary (citable) accession number: Q8N8N7
Secondary accession number(s): Q3L8A4, Q6MZH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.