Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Prostaglandin reductase 2

Gene

PTGR2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation (By similarity).By similarity1 Publication

Catalytic activityi

11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.1 Publication

Kineticsi

  1. KM=11.21 µM for 15-keto-PGE21 Publication
  2. KM=15.87 µM for NADPH1 Publication
  1. Vmax=159 nmol/min/mg enzyme for 15-keto-PGE21 Publication
  2. Vmax=67 nmol/min/mg enzyme for NADPH1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei192NADP1
Binding sitei208NADP1
Binding sitei231NADP1
Binding sitei337NADP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 168NADP4
Nucleotide bindingi253 – 259NADP7
Nucleotide bindingi287 – 289NADP3

GO - Molecular functioni

  • 13-prostaglandin reductase activity Source: UniProtKB-EC
  • 15-oxoprostaglandin 13-oxidase activity Source: UniProtKB

GO - Biological processi

  • prostaglandin metabolic process Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

ReactomeiR-HSA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)

Chemistry databases

SwissLipidsiSLP:000001640 [Q8N8N7-1]

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin reductase 2 (EC:1.3.1.48)
Short name:
PRG-2
Alternative name(s):
15-oxoprostaglandin 13-reductase
Zinc-binding alcohol dehydrogenase domain-containing protein 1
Gene namesi
Name:PTGR2
Synonyms:ZADH1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000140043.11
HGNCiHGNC:20149 PTGR2
MIMi608642 gene
neXtProtiNX_Q8N8N7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi64Y → F: Increases affinity for 15-keto-PGE2. Reduces affinity for NADP and Vmax. 1 Publication1
Mutagenesisi259Y → F: Increases affinity for 15-keto-PGE2. Reduces affinity for NADP and Vmax. 1 Publication1

Organism-specific databases

DisGeNETi145482
OpenTargetsiENSG00000140043
PharmGKBiPA162400323

Chemistry databases

DrugBankiDB00328 Indomethacin

Polymorphism and mutation databases

BioMutaiPTGR2
DMDMi62901454

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002180701 – 351Prostaglandin reductase 2Add BLAST351

Proteomic databases

EPDiQ8N8N7
PaxDbiQ8N8N7
PeptideAtlasiQ8N8N7
PRIDEiQ8N8N7

2D gel databases

REPRODUCTION-2DPAGEiIPI00167515

PTM databases

iPTMnetiQ8N8N7
PhosphoSitePlusiQ8N8N7

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000140043
CleanExiHS_PTGR2
ExpressionAtlasiQ8N8N7 baseline and differential
GenevisibleiQ8N8N7 HS

Organism-specific databases

HPAiHPA001125

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi126915, 15 interactors
STRINGi9606.ENSP00000267568

Structurei

Secondary structure

1351
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Helixi20 – 22Combined sources3
Beta strandi23 – 29Combined sources7
Beta strandi38 – 47Combined sources10
Helixi52 – 55Combined sources4
Beta strandi57 – 59Combined sources3
Beta strandi61 – 65Combined sources5
Beta strandi75 – 85Combined sources11
Beta strandi95 – 110Combined sources16
Helixi111 – 113Combined sources3
Beta strandi115 – 117Combined sources3
Helixi119 – 122Combined sources4
Helixi126 – 130Combined sources5
Turni131 – 133Combined sources3
Helixi135 – 147Combined sources13
Beta strandi157 – 162Combined sources6
Helixi166 – 177Combined sources12
Beta strandi181 – 188Combined sources8
Helixi190 – 198Combined sources9
Beta strandi203 – 207Combined sources5
Turni208 – 210Combined sources3
Helixi213 – 220Combined sources8
Beta strandi225 – 231Combined sources7
Helixi234 – 242Combined sources9
Beta strandi244 – 252Combined sources9
Helixi256 – 258Combined sources3
Helixi271 – 280Combined sources10
Beta strandi283 – 286Combined sources4
Helixi289 – 295Combined sources7
Helixi296 – 308Combined sources13
Beta strandi316 – 320Combined sources5
Helixi322 – 324Combined sources3
Helixi325 – 333Combined sources9
Beta strandi338 – 344Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VNAX-ray2.17A1-349[»]
2W4QX-ray2.00A1-349[»]
2W98X-ray1.85A/B1-349[»]
2ZB4X-ray1.63A1-351[»]
2ZB7X-ray1.80A1-351[»]
2ZB8X-ray2.00A1-351[»]
ProteinModelPortaliQ8N8N7
SMRiQ8N8N7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8N8N7

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni99 – 100Substrate binding2
Regioni288 – 290Substrate binding3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1196 Eukaryota
COG2130 LUCA
GeneTreeiENSGT00390000009335
HOGENOMiHOG000294663
HOVERGENiHBG055024
InParanoidiQ8N8N7
KOiK13949
OMAiWMSDIPQ
OrthoDBiEOG091G0F5B
PhylomeDBiQ8N8N7
TreeFamiTF324201

Family and domain databases

CDDicd08293 PTGR2, 1 hit
InterProiView protein in InterPro
IPR013149 ADH_C
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR037399 PTGR2
PfamiView protein in Pfam
PF00107 ADH_zinc_N, 1 hit
SUPFAMiSSF50129 SSF50129, 2 hits
SSF51735 SSF51735, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N8N7-1) [UniParc]FASTAAdd to basket
Also known as: ZADH1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIVQRVVLNS RPGKNGNPVA ENFRMEEVYL PDNINEGQVQ VRTLYLSVDP
60 70 80 90 100
YMRCRMNEDT GTDYITPWQL SQVVDGGGIG IIEESKHTNL TKGDFVTSFY
110 120 130 140 150
WPWQTKVILD GNSLEKVDPQ LVDGHLSYFL GAIGMPGLTS LIGIQEKGHI
160 170 180 190 200
TAGSNKTMVV SGAAGACGSV AGQIGHFLGC SRVVGICGTH EKCILLTSEL
210 220 230 240 250
GFDAAINYKK DNVAEQLRES CPAGVDVYFD NVGGNISDTV ISQMNENSHI
260 270 280 290 300
ILCGQISQYN KDVPYPPPLS PAIEAIQKER NITRERFLVL NYKDKFEPGI
310 320 330 340 350
LQLSQWFKEG KLKIKETVIN GLENMGAAFQ SMMTGGNIGK QIVCISEEIS

L
Length:351
Mass (Da):38,499
Last modified:October 1, 2002 - v1
Checksum:i118ED6D2FB984F3A
GO
Isoform 2 (identifier: Q8N8N7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     174-180: IGHFLGC → VNFLRII
     181-351: Missing.

Note: No experimental confirmation available.
Show »
Length:180
Mass (Da):19,675
Checksum:iB2E6EB6A71991004
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013526174 – 180IGHFLGC → VNFLRII in isoform 2. 1 Publication7
Alternative sequenceiVSP_013527181 – 351Missing in isoform 2. 1 PublicationAdd BLAST171

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY346133 mRNA Translation: AAR05101.1
AY424308 mRNA Translation: AAR82927.1
AK096410 mRNA Translation: BAC04781.1
BX641118 mRNA Translation: CAE46055.1
CH471061 Genomic DNA Translation: EAW81132.1
BC059364 mRNA Translation: AAH59364.1
CCDSiCCDS9820.1 [Q8N8N7-1]
RefSeqiNP_001139626.1, NM_001146154.1 [Q8N8N7-1]
NP_001139627.1, NM_001146155.1 [Q8N8N7-1]
NP_689657.1, NM_152444.2 [Q8N8N7-1]
UniGeneiHs.632344

Genome annotation databases

EnsembliENST00000267568; ENSP00000267568; ENSG00000140043 [Q8N8N7-1]
ENST00000555228; ENSP00000450975; ENSG00000140043 [Q8N8N7-1]
ENST00000555661; ENSP00000452280; ENSG00000140043 [Q8N8N7-1]
GeneIDi145482
KEGGihsa:145482

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPTGR2_HUMAN
AccessioniPrimary (citable) accession number: Q8N8N7
Secondary accession number(s): Q3L8A4, Q6MZH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health