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Protein

Prostaglandin reductase 2

Gene

PTGR2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation (By similarity).By similarity1 Publication

Catalytic activityi

11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.1 Publication

Kineticsi

  1. KM=11.21 µM for 15-keto-PGE21 Publication
  2. KM=15.87 µM for NADPH1 Publication
  1. Vmax=159 nmol/min/mg enzyme for 15-keto-PGE21 Publication
  2. Vmax=67 nmol/min/mg enzyme for NADPH1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei192 – 1921NADP
Binding sitei208 – 2081NADP
Binding sitei231 – 2311NADP
Binding sitei337 – 3371NADP

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi165 – 1684NADP
Nucleotide bindingi253 – 2597NADP
Nucleotide bindingi287 – 2893NADP

GO - Molecular functioni

  • 13-prostaglandin reductase activity Source: UniProtKB-EC
  • 15-oxoprostaglandin 13-oxidase activity Source: UniProtKB

GO - Biological processi

  • prostaglandin metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin reductase 2 (EC:1.3.1.48)
Short name:
PRG-2
Alternative name(s):
15-oxoprostaglandin 13-reductase
Zinc-binding alcohol dehydrogenase domain-containing protein 1
Gene namesi
Name:PTGR2
Synonyms:ZADH1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:20149. PTGR2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi64 – 641Y → F: Increases affinity for 15-keto-PGE2. Reduces affinity for NADP and Vmax. 1 Publication
Mutagenesisi259 – 2591Y → F: Increases affinity for 15-keto-PGE2. Reduces affinity for NADP and Vmax. 1 Publication

Organism-specific databases

PharmGKBiPA162400323.

Chemistry

DrugBankiDB00328. Indomethacin.

Polymorphism and mutation databases

BioMutaiPTGR2.
DMDMi62901454.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Prostaglandin reductase 2PRO_0000218070Add
BLAST

Proteomic databases

EPDiQ8N8N7.
PaxDbiQ8N8N7.
PRIDEiQ8N8N7.

2D gel databases

REPRODUCTION-2DPAGEIPI00167515.

PTM databases

iPTMnetiQ8N8N7.
PhosphoSiteiQ8N8N7.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiQ8N8N7.
CleanExiHS_PTGR2.
ExpressionAtlasiQ8N8N7. baseline and differential.
GenevisibleiQ8N8N7. HS.

Organism-specific databases

HPAiHPA001125.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi126915. 15 interactions.
STRINGi9606.ENSP00000267568.

Structurei

Secondary structure

1
351
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87Combined sources
Helixi20 – 223Combined sources
Beta strandi23 – 297Combined sources
Beta strandi38 – 4710Combined sources
Helixi52 – 554Combined sources
Beta strandi57 – 593Combined sources
Beta strandi61 – 655Combined sources
Beta strandi75 – 8511Combined sources
Beta strandi95 – 11016Combined sources
Helixi111 – 1133Combined sources
Beta strandi115 – 1173Combined sources
Helixi119 – 1224Combined sources
Helixi126 – 1305Combined sources
Turni131 – 1333Combined sources
Helixi135 – 14713Combined sources
Beta strandi157 – 1626Combined sources
Helixi166 – 17712Combined sources
Beta strandi181 – 1888Combined sources
Helixi190 – 1989Combined sources
Beta strandi203 – 2075Combined sources
Turni208 – 2103Combined sources
Helixi213 – 2208Combined sources
Beta strandi225 – 2317Combined sources
Helixi234 – 2429Combined sources
Beta strandi244 – 2529Combined sources
Helixi256 – 2583Combined sources
Helixi271 – 28010Combined sources
Beta strandi283 – 2864Combined sources
Helixi289 – 2957Combined sources
Helixi296 – 30813Combined sources
Beta strandi316 – 3205Combined sources
Helixi322 – 3243Combined sources
Helixi325 – 3339Combined sources
Beta strandi338 – 3447Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VNAX-ray2.17A1-349[»]
2W4QX-ray2.00A1-349[»]
2W98X-ray1.85A/B1-349[»]
2ZB4X-ray1.63A1-351[»]
2ZB7X-ray1.80A1-351[»]
2ZB8X-ray2.00A1-351[»]
ProteinModelPortaliQ8N8N7.
SMRiQ8N8N7. Positions 1-350.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8N8N7.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni99 – 1002Substrate binding
Regioni288 – 2903Substrate binding

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1196. Eukaryota.
COG2130. LUCA.
HOGENOMiHOG000294663.
HOVERGENiHBG055024.
InParanoidiQ8N8N7.
KOiK13949.
OMAiESKHTNF.
OrthoDBiEOG7B31N5.
PhylomeDBiQ8N8N7.
TreeFamiTF324201.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N8N7-1) [UniParc]FASTAAdd to basket

Also known as: ZADH1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIVQRVVLNS RPGKNGNPVA ENFRMEEVYL PDNINEGQVQ VRTLYLSVDP
60 70 80 90 100
YMRCRMNEDT GTDYITPWQL SQVVDGGGIG IIEESKHTNL TKGDFVTSFY
110 120 130 140 150
WPWQTKVILD GNSLEKVDPQ LVDGHLSYFL GAIGMPGLTS LIGIQEKGHI
160 170 180 190 200
TAGSNKTMVV SGAAGACGSV AGQIGHFLGC SRVVGICGTH EKCILLTSEL
210 220 230 240 250
GFDAAINYKK DNVAEQLRES CPAGVDVYFD NVGGNISDTV ISQMNENSHI
260 270 280 290 300
ILCGQISQYN KDVPYPPPLS PAIEAIQKER NITRERFLVL NYKDKFEPGI
310 320 330 340 350
LQLSQWFKEG KLKIKETVIN GLENMGAAFQ SMMTGGNIGK QIVCISEEIS

L
Length:351
Mass (Da):38,499
Last modified:October 1, 2002 - v1
Checksum:i118ED6D2FB984F3A
GO
Isoform 2 (identifier: Q8N8N7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     174-180: IGHFLGC → VNFLRII
     181-351: Missing.

Note: No experimental confirmation available.
Show »
Length:180
Mass (Da):19,675
Checksum:iB2E6EB6A71991004
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei174 – 1807IGHFLGC → VNFLRII in isoform 2. 1 PublicationVSP_013526
Alternative sequencei181 – 351171Missing in isoform 2. 1 PublicationVSP_013527Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY346133 mRNA. Translation: AAR05101.1.
AY424308 mRNA. Translation: AAR82927.1.
AK096410 mRNA. Translation: BAC04781.1.
BX641118 mRNA. Translation: CAE46055.1.
CH471061 Genomic DNA. Translation: EAW81132.1.
BC059364 mRNA. Translation: AAH59364.1.
CCDSiCCDS9820.1. [Q8N8N7-1]
RefSeqiNP_001139626.1. NM_001146154.1. [Q8N8N7-1]
NP_001139627.1. NM_001146155.1. [Q8N8N7-1]
NP_689657.1. NM_152444.2. [Q8N8N7-1]
UniGeneiHs.632344.

Genome annotation databases

EnsembliENST00000267568; ENSP00000267568; ENSG00000140043. [Q8N8N7-1]
ENST00000555228; ENSP00000450975; ENSG00000140043. [Q8N8N7-1]
ENST00000555661; ENSP00000452280; ENSG00000140043. [Q8N8N7-1]
GeneIDi145482.
KEGGihsa:145482.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY346133 mRNA. Translation: AAR05101.1.
AY424308 mRNA. Translation: AAR82927.1.
AK096410 mRNA. Translation: BAC04781.1.
BX641118 mRNA. Translation: CAE46055.1.
CH471061 Genomic DNA. Translation: EAW81132.1.
BC059364 mRNA. Translation: AAH59364.1.
CCDSiCCDS9820.1. [Q8N8N7-1]
RefSeqiNP_001139626.1. NM_001146154.1. [Q8N8N7-1]
NP_001139627.1. NM_001146155.1. [Q8N8N7-1]
NP_689657.1. NM_152444.2. [Q8N8N7-1]
UniGeneiHs.632344.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VNAX-ray2.17A1-349[»]
2W4QX-ray2.00A1-349[»]
2W98X-ray1.85A/B1-349[»]
2ZB4X-ray1.63A1-351[»]
2ZB7X-ray1.80A1-351[»]
2ZB8X-ray2.00A1-351[»]
ProteinModelPortaliQ8N8N7.
SMRiQ8N8N7. Positions 1-350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126915. 15 interactions.
STRINGi9606.ENSP00000267568.

Chemistry

DrugBankiDB00328. Indomethacin.

PTM databases

iPTMnetiQ8N8N7.
PhosphoSiteiQ8N8N7.

Polymorphism and mutation databases

BioMutaiPTGR2.
DMDMi62901454.

2D gel databases

REPRODUCTION-2DPAGEIPI00167515.

Proteomic databases

EPDiQ8N8N7.
PaxDbiQ8N8N7.
PRIDEiQ8N8N7.

Protocols and materials databases

DNASUi145482.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000267568; ENSP00000267568; ENSG00000140043. [Q8N8N7-1]
ENST00000555228; ENSP00000450975; ENSG00000140043. [Q8N8N7-1]
ENST00000555661; ENSP00000452280; ENSG00000140043. [Q8N8N7-1]
GeneIDi145482.
KEGGihsa:145482.

Organism-specific databases

CTDi145482.
GeneCardsiPTGR2.
HGNCiHGNC:20149. PTGR2.
HPAiHPA001125.
MIMi608642. gene.
neXtProtiNX_Q8N8N7.
PharmGKBiPA162400323.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1196. Eukaryota.
COG2130. LUCA.
HOGENOMiHOG000294663.
HOVERGENiHBG055024.
InParanoidiQ8N8N7.
KOiK13949.
OMAiESKHTNF.
OrthoDBiEOG7B31N5.
PhylomeDBiQ8N8N7.
TreeFamiTF324201.

Miscellaneous databases

ChiTaRSiPTGR2. human.
EvolutionaryTraceiQ8N8N7.
GenomeRNAii145482.
NextBioi85108.
PROiQ8N8N7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8N8N7.
CleanExiHS_PTGR2.
ExpressionAtlasiQ8N8N7. baseline and differential.
GenevisibleiQ8N8N7. HS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a novel splicing variant of the ZADH1 gene."
    Zhang L., Zhang F., Huo K.
    Cytogenet. Genome Res. 103:79-83(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
  2. "Cloning and characterization of a putative dehydrogenase."
    Zheng H., Xie Y., Mao Y.
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Fetal kidney.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. "Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2."
    Wu Y.H., Ko T.P., Guo R.T., Hu S.M., Chuang L.M., Wang A.H.
    Structure 16:1714-1723(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.63 ANGSTROMS) IN COMPLEXES WITH NADPH; 15-KETO-PGE2 AND INDOMETHACIN, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF TYR-64 AND TYR-259.
  10. "Crystal structure of human zinc-binding alcohol dehydrogenase 1."
    Structural genomics consortium (SGC)
    Submitted (JAN-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEXES WITH SUBSTRATE ANALOGS AND NADP.

Entry informationi

Entry nameiPTGR2_HUMAN
AccessioniPrimary (citable) accession number: Q8N8N7
Secondary accession number(s): Q3L8A4, Q6MZH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 1, 2002
Last modified: April 13, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.