Reviewed, UniProtKB/Swiss-Prot Q8N8N7 (PTGR2_HUMAN)

Names and origin

Protein names

Recommended name:
    Prostaglandin reductase 2
      Short name=PRG-2
    EC=1.3.1.48
Alternative name(s):
    15-oxoprostaglandin 13-reductase
    Zinc-binding alcohol dehydrogenase domain-containing protein 1

Gene names

Name:	PTGR2
Synonyms:	ZADH1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation By similarity.
Catalytic activity	11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.
Cofactor	NADPH By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Widely expressed. Ref.1
Sequence similarities	Belongs to the NADP-dependent oxidoreductase L4BD family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Non-traceable author statement. Source: UniProtKB nucleus Inferred from direct assay. Source: HPA
Molecular function	15-oxoprostaglandin 13-oxidase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N8N7-1)

Also known as: ZADH1b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N8N7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     174-180: IGHFLGC → VNFLRII
     181-351: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 351

351

Prostaglandin reductase 2

PRO_0000218070

Regions

Nucleotide binding

162 – 168

7

NADP Potential

Nucleotide binding

188 – 208

21

NADP Potential

Natural variations

Alternative sequence

174 – 180

7

IGHFLGC → VNFLRII in isoform 2.

VSP_013526

Alternative sequence

181 – 351

171

Missing in isoform 2.

VSP_013527

Secondary structure

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351

Helix Strand Turn

Details...

Beta strand

2 – 8

7

Helix

20 – 22

3

Beta strand

23 – 29

7

Beta strand

39 – 42

4

Beta strand

45 – 47

3

Helix

52 – 55

4

Beta strand

82 – 85

4

Beta strand

103 – 109

7

Helix

119 – 122

4

Helix

126 – 129

4

Turn

139 – 141

3

Beta strand

157 – 160

4

Beta strand

182 – 185

4

Helix

190 – 198

9

Turn

208 – 210

3

Helix

213 – 220

8

Beta strand

227 – 230

4

Helix

236 – 242

7

Beta strand

249 – 252

4

Helix

256 – 258

3

Helix

271 – 280

10

Beta strand

283 – 286

4

Helix

289 – 295

7

Helix

301 – 308

8

Beta strand

317 – 319

3

Helix

328 – 333

6

Beta strand

341 – 344

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (ZADH1b) [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 118ED6D2FB984F3A	FASTA	351	38,499
	10 20 30 40 50 60 MIVQRVVLNS RPGKNGNPVA ENFRMEEVYL PDNINEGQVQ VRTLYLSVDP YMRCRMNEDT 70 80 90 100 110 120 GTDYITPWQL SQVVDGGGIG IIEESKHTNL TKGDFVTSFY WPWQTKVILD GNSLEKVDPQ 130 140 150 160 170 180 LVDGHLSYFL GAIGMPGLTS LIGIQEKGHI TAGSNKTMVV SGAAGACGSV AGQIGHFLGC 190 200 210 220 230 240 SRVVGICGTH EKCILLTSEL GFDAAINYKK DNVAEQLRES CPAGVDVYFD NVGGNISDTV 250 260 270 280 290 300 ISQMNENSHI ILCGQISQYN KDVPYPPPLS PAIEAIQKER NITRERFLVL NYKDKFEPGI 310 320 330 340 350 LQLSQWFKEG KLKIKETVIN GLENMGAAFQ SMMTGGNIGK QIVCISEEIS L
	Isoform 2. Checksum: B2E6EB6A71991004	FASTA	180	19,675
	10 20 30 40 50 60 MIVQRVVLNS RPGKNGNPVA ENFRMEEVYL PDNINEGQVQ VRTLYLSVDP YMRCRMNEDT 70 80 90 100 110 120 GTDYITPWQL SQVVDGGGIG IIEESKHTNL TKGDFVTSFY WPWQTKVILD GNSLEKVDPQ 130 140 150 160 170 180 LVDGHLSYFL GAIGMPGLTS LIGIQEKGHI TAGSNKTMVV SGAAGACGSV AGQVNFLRII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a novel splicing variant of the ZADH1 gene." Zhang L., Zhang F., Huo K. Cytogenet. Genome Res. 103:79-83(2003) [PubMed: 15004468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
[2]	"Cloning and characterization of a putative dehydrogenase." Zheng H., Xie Y., Mao Y. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Fetal kidney.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[7]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AY346133 mRNA. Translation: AAR05101.1.
AY424308 mRNA. Translation: AAR82927.1.
AK096410 mRNA. Translation: BAC04781.1.
BX641118 mRNA. Translation: CAE46055.1.
CH471061 Genomic DNA. Translation: EAW81132.1.
BC059364 mRNA. Translation: AAH59364.1.

IPI

IPI00167515.
IPI00869302.

RefSeq

NP_001139626.1.
NP_001139627.1.
NP_689657.1.

UniGene

Hs.632344

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VNA	X-ray	2.17	A	1-349	[»]
2W4Q	X-ray	2.00	A	1-349	[»]
2W98	X-ray	1.85	A/B	1-349	[»]
2ZB4	X-ray	1.63	A	1-351	[»]
2ZB7	X-ray	1.80	A	1-351	[»]
2ZB8	X-ray	2.00	A	1-351	[»]

SMR

Q8N8N7. Positions 1-351.

ModBase

Search...

Protein-protein interaction databases

STRING

Q8N8N7.

2-D gel databases

REPRODUCTION-2DPAGE

IPI00167515.

Proteomic databases

PRIDE

Q8N8N7.

Genome annotation databases

Ensembl

ENST00000267568; ENSP00000267568; ENSG00000140043; Homo sapiens. [Genome view]

GeneID

145482.

KEGG

hsa:145482.

UCSC

uc001xow.1. human.

Organism-specific databases

CTD

145482.

GeneCards

GC14P073389.

H-InvDB

HIX0079548.
HIX0079612.

HGNC

HGNC:20149. PTGR2.

HPA

HPA000695.

MIM

608642. gene.

PharmGKB

PA134976517.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q8N8N7.

HOVERGEN

Q8N8N7.

OMA

GLENMGV.

Gene expression databases

ArrayExpress

Q8N8N7.

Bgee

Q8N8N7.

CleanEx

HS_PTGR2.

Genevestigator

Q8N8N7.

Family and domain databases

InterPro

IPR002085. ADH_SF_Zn.
IPR013149. ADH_Zn-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR11695. ADH_Sf_Zn. 1 hit.

Pfam

PF00107. ADH_zinc_N. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

85108.

SOURCE

Search...

Entry information

Entry name

PTGR2_HUMAN

Accession

Primary (citable) accession number: Q8N8N7
Secondary accession number(s): Q3L8A4, Q6MZH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.