Q8N895 (ZN366_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 366

Gene names

Name:

ZNF366

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	744 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has transcriptional repression activity. Acts as corepressor of ESR1; the function seems to involve CTBP1 and histone deacetylases. Ref.6
Subunit structure	Interacts with ESR1, NRIP1 and CTBP1. Ref.6
Subcellular location	Nucleus Ref.6.
Sequence similarities	Contains 11 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of intracellular estrogen receptor signaling pathway Inferred from direct assay Ref.6. Source: UniProtKB negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.6. Source: UniProtKB response to estrogen stimulus Inferred from direct assay Ref.6. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay Ref.6. Source: UniProtKB
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW estrogen receptor binding Inferred from direct assay Ref.6. Source: UniProtKB protein binding transcription factor activity Inferred from direct assay Ref.6. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CTBP1	Q13363	5	EBI-2813661,EBI-908846
ESR1	P03372	6	EBI-2813661,EBI-78473
NRIP1	P48552	2	EBI-2813661,EBI-746484

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 744	744	Zinc finger protein 366		PRO_0000047548
Regions
	Zinc finger	253 – 275	23	C2H2-type 1
	Zinc finger	281 – 303	23	C2H2-type 2
	Zinc finger	309 – 331	23	C2H2-type 3
	Zinc finger	337 – 359	23	C2H2-type 4
	Zinc finger	365 – 387	23	C2H2-type 5
	Zinc finger	393 – 415	23	C2H2-type 6
	Zinc finger	421 – 443	23	C2H2-type 7
	Zinc finger	449 – 471	23	C2H2-type 8
	Zinc finger	477 – 499	23	C2H2-type 9
	Zinc finger	505 – 527	23	C2H2-type 10
	Zinc finger	533 – 556	24	C2H2-type 11
	Region	455 – 744	290	Interaction with NRIP1
	Region	558 – 744	187	Interaction with CTBP1
Natural variations
	Natural variant	739	1	A → G. Corresponds to variant rs13188519 [ dbSNP | Ensembl ].		VAR_033564
Experimental info
	Mutagenesis	590	1	P → A: Abolishes interaction with CTBP1 and in part relieves transcription repression; when associated with A-592. Ref.6
	Mutagenesis	592	1	D → A: Abolishes interaction with CTBP1 and in part relieves transcription repression; when associated with A-590. Ref.6
	Mutagenesis	645	1	P → A: Decreases interaction with CTBP1; when associated with A-647. Ref.6
	Mutagenesis	647	1	D → A: Decreases interaction with CTBP1; when associated with A-645. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q8N895 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: D4E12259280727B1	FASTA	744	85,107
	10 20 30 40 50 60 MQKEMKMIKD EDVHFDLAVK KTPSFPHCLQ PVASRGKAPQ RHPFPEALRG PFSQFRYEPP 70 80 90 100 110 120 PGDLDGFPGV FEGAGSRKRK SMPTKMPYNH PAEEVTLALH SEENKNHGLP NLPLLFPQPP 130 140 150 160 170 180 RPKYDSQMID LCNVGFQFYR SLEHFGGKPV KQEPIKPSAV WPQPTPTPFL PTPYPYYPKV 190 200 210 220 230 240 HPGLMFPFFV PSSSPFPFSR HTFLPKQPPE PLLPRKAEPQ ESEETKQKVE RVDVNVQIDD 250 260 270 280 290 300 SYYVDVGGSQ KRWQCPTCEK SYTSKYNLVT HILGHSGIKP HACTHCGKLF KQLSHLHTHM 310 320 330 340 350 360 LTHQGTRPHK CQVCHKAFTQ TSHLKRHMMQ HSEVKPHNCR VCGRGFAYPS ELKAHEAKHA 370 380 390 400 410 420 SGRENICVEC GLDFPTLAQL KRHLTTHRGP IQYNCSECDK TFQYPSQLQN HMMKHKDIRP 430 440 450 460 470 480 YICSECGMEF VQPHHLKQHS LTHKGVKEHK CGICGREFTL LANMKRHVLI HTNIRAYQCH 490 500 510 520 530 540 LCYKSFVQKQ TLKAHMIVHS DVKPFKCKLC GKEFNRMHNL MGHMHLHSDS KPFKCLYCPS 550 560 570 580 590 600 KFTLKGNLTR HMKVKHGVME RGLHSQGLGR GRIALAQTAG VLRSLEQEEP FDLSQKRRAK 610 620 630 640 650 660 VPVFQSDGES AQGSHCHEEE EEDNCYEVEP YSPGLAPQSQ QLCTPEDLST KSEHAPEVLE 670 680 690 700 710 720 EACKEEKEDA SKGEWEKRSK GDLGAEGGQE RDCAGRDECL SLRAFQSTRR GPSFSDYLYF 730 740 KHRDESLKEL LERKMEKQAV LLGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[3]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Fugu and human sequence comparison identifies novel human genes and conserved non-coding sequences." Gilligan P., Brenner S., Venkatesh B. Gene 294:35-44(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[6]	"ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases." Lopez-Garcia J., Periyasamy M., Thomas R.S., Christian M., Leao M., Jat P., Kindle K.B., Heery D.M., Parker M.G., Buluwela L., Kamalati T., Ali S. Nucleic Acids Res. 34:6126-6136(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ESR1; NRIP1 AND CTBP1, MUTAGENESIS OF PRO-590; ASP-592; PRO-645 AND ASP-647.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK097115 mRNA. Translation: BAC04954.1. BX647592 mRNA. Translation: CAI46096.1. CH471084 Genomic DNA. Translation: EAW95707.1. BC121053 mRNA. Translation: AAI21054.1. BK000210 mRNA. Translation: DAA00066.1.
IPI	IPI00167408.
RefSeq	NP_689838.1. NM_152625.1.
UniGene	Hs.370303.
3D structure databases
ProteinModelPortal	Q8N895.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N895. 5 interactions.
STRING	9606.ENSP00000313158.
PTM databases
PhosphoSite	Q8N895.
Polymorphism databases
DMDM	28380235.
Proteomic databases
PaxDb	Q8N895.
PRIDE	Q8N895.
Protocols and materials databases
DNASU	167465.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000318442; ENSP00000313158; ENSG00000178175.
GeneID	167465.
KEGG	hsa:167465.
UCSC	uc003kce.1. human.
Organism-specific databases
CTD	167465.
GeneCards	GC05M071774.
HGNC	HGNC:18316. ZNF366.
HPA	HPA023128. HPA023526.
MIM	610159. gene.
neXtProt	NX_Q8N895.
PharmGKB	PA38314.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000138030.
HOVERGEN	HBG055962.
InParanoid	Q8N895.
OMA	HMMQHSE.
OrthoDB	EOG4ZKJM4.
PhylomeDB	Q8N895.
Gene expression databases
ArrayExpress	Q8N895.
Bgee	Q8N895.
CleanEx	HS_ZNF366.
Genevestigator	Q8N895.
GermOnline	ENSG00000178175. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 10 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF00096. zf-C2H2. 3 hits. [Graphical view]
SMART	SM00355. ZnF_C2H2. 11 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 11 hits. PS50157. ZINC_FINGER_C2H2_2. 11 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	167465.
NextBio	88674.
SOURCE	Search...

Entry information

Entry name

ZN366_HUMAN

Accession

Primary (citable) accession number: Q8N895
Secondary accession number(s): Q5HYI9, Q7RTV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.