ID   PPM1N_HUMAN             Reviewed;         430 AA.
AC   Q8N819; Q6P662;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 2.
DT   27-MAR-2024, entry version 150.
DE   RecName: Full=Probable protein phosphatase 1N;
DE            EC=3.1.3.16;
GN   Name=PPM1N;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15057824; DOI=10.1038/nature02399;
RA   Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA   Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA   Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA   Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA   Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA   Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA   Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA   Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA   Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA   McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA   Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA   Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA   She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA   Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA   Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA   Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA   Rubin E.M., Lucas S.M.;
RT   "The DNA sequence and biology of human chromosome 19.";
RL   Nature 428:529-535(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:83421; EC=3.1.3.16;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC         COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:61977; EC=3.1.3.16;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250};
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q8N819-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8N819-2; Sequence=VSP_035234, VSP_035235;
CC       Name=3;
CC         IsoId=Q8N819-3; Sequence=VSP_035233;
CC   -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
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DR   EMBL; AC138534; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471126; EAW57360.1; -; Genomic_DNA.
DR   EMBL; AK097444; BAC05056.1; -; mRNA.
DR   EMBL; BC028228; AAH28228.1; -; mRNA.
DR   EMBL; BC062452; AAH62452.1; -; mRNA.
DR   CCDS; CCDS46115.1; -. [Q8N819-1]
DR   RefSeq; NP_001073870.1; NM_001080401.1. [Q8N819-1]
DR   AlphaFoldDB; Q8N819; -.
DR   SMR; Q8N819; -.
DR   BioGRID; 127077; 2.
DR   IntAct; Q8N819; 1.
DR   STRING; 9606.ENSP00000397050; -.
DR   DEPOD; PPM1N; -.
DR   BioMuta; PPM1N; -.
DR   DMDM; 205829293; -.
DR   MassIVE; Q8N819; -.
DR   PaxDb; 9606-ENSP00000397050; -.
DR   PeptideAtlas; Q8N819; -.
DR   ProteomicsDB; 72364; -. [Q8N819-1]
DR   ProteomicsDB; 72365; -. [Q8N819-2]
DR   Antibodypedia; 31351; 10 antibodies from 6 providers.
DR   DNASU; 147699; -.
DR   Ensembl; ENST00000396735.6; ENSP00000379961.2; ENSG00000213889.11. [Q8N819-3]
DR   Ensembl; ENST00000396737.6; ENSP00000379963.1; ENSG00000213889.11. [Q8N819-3]
DR   Ensembl; ENST00000401705.5; ENSP00000384318.1; ENSG00000213889.11. [Q8N819-3]
DR   Ensembl; ENST00000451287.7; ENSP00000397050.2; ENSG00000213889.11. [Q8N819-1]
DR   GeneID; 147699; -.
DR   KEGG; hsa:147699; -.
DR   MANE-Select; ENST00000451287.7; ENSP00000397050.2; NM_001080401.2; NP_001073870.1.
DR   UCSC; uc002pce.4; human. [Q8N819-1]
DR   AGR; HGNC:26845; -.
DR   CTD; 147699; -.
DR   DisGeNET; 147699; -.
DR   GeneCards; PPM1N; -.
DR   HGNC; HGNC:26845; PPM1N.
DR   HPA; ENSG00000213889; Tissue enriched (skeletal).
DR   neXtProt; NX_Q8N819; -.
DR   OpenTargets; ENSG00000213889; -.
DR   PharmGKB; PA165394107; -.
DR   VEuPathDB; HostDB:ENSG00000213889; -.
DR   eggNOG; KOG0697; Eukaryota.
DR   GeneTree; ENSGT00940000162694; -.
DR   HOGENOM; CLU_146811_0_0_1; -.
DR   InParanoid; Q8N819; -.
DR   OMA; LWTACKK; -.
DR   OrthoDB; 11028at2759; -.
DR   PhylomeDB; Q8N819; -.
DR   PathwayCommons; Q8N819; -.
DR   SignaLink; Q8N819; -.
DR   BioGRID-ORCS; 147699; 12 hits in 1166 CRISPR screens.
DR   ChiTaRS; PPM1N; human.
DR   GenomeRNAi; 147699; -.
DR   Pharos; Q8N819; Tdark.
DR   PRO; PR:Q8N819; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   RNAct; Q8N819; Protein.
DR   Bgee; ENSG00000213889; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 95 other cell types or tissues.
DR   ExpressionAtlas; Q8N819; baseline and differential.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004722; F:protein serine/threonine phosphatase activity; IBA:GO_Central.
DR   GO; GO:0043124; P:negative regulation of canonical NF-kappaB signal transduction; IBA:GO_Central.
DR   GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; IBA:GO_Central.
DR   CDD; cd00143; PP2Cc; 1.
DR   Gene3D; 1.10.10.430; Phosphatase 2C, C-terminal domain suprefamily; 1.
DR   Gene3D; 3.60.40.10; PPM-type phosphatase domain; 1.
DR   InterPro; IPR015655; PP2C.
DR   InterPro; IPR012911; PP2C_C.
DR   InterPro; IPR036580; PP2C_C_sf.
DR   InterPro; IPR036457; PPM-type-like_dom_sf.
DR   InterPro; IPR001932; PPM-type_phosphatase-like_dom.
DR   PANTHER; PTHR47992; PROTEIN PHOSPHATASE; 1.
DR   PANTHER; PTHR47992:SF59; PROTEIN PHOSPHATASE 1N-RELATED; 1.
DR   Pfam; PF00481; PP2C; 1.
DR   Pfam; PF07830; PP2C_C; 1.
DR   SMART; SM00332; PP2Cc; 1.
DR   SUPFAM; SSF81606; PP2C-like; 1.
DR   SUPFAM; SSF81601; Protein serine/threonine phosphatase 2C, C-terminal domain; 1.
DR   PROSITE; PS51746; PPM_2; 1.
DR   Genevisible; Q8N819; HS.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Hydrolase; Magnesium; Manganese; Metal-binding;
KW   Protein phosphatase; Reference proteome.
FT   CHAIN           1..430
FT                   /note="Probable protein phosphatase 1N"
FT                   /id="PRO_0000349231"
FT   DOMAIN          66..326
FT                   /note="PPM-type phosphatase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT   REGION          16..65
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          407..430
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        16..39
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         103
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         103
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         104
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         274
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         317
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         1..318
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_035233"
FT   VAR_SEQ         1..78
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039,
FT                   ECO:0000303|PubMed:15489334"
FT                   /id="VSP_035234"
FT   VAR_SEQ         314..430
FT                   /note="GSLDNMTCILVCFPGAPRPSEEAIRRELALDAALGCRIAELCASAQKPPSLN
FT                   TVFRTLASEDIPDLPPGGGLDCKATVIAEVYSQICQVSEECGEKGQDGAGKSNPTHLGS
FT                   ALDMEA -> VLGAWRGTFGAWCSRGREPRGFGEEGFDREARVKLAKEGIGLKRRAWPE
FT                   VGRAKIQGRSLRDALRHGRVSGRDLRERAWSFGKGRFWVVGAGPEVLISAGRWKSR
FT                   (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039,
FT                   ECO:0000303|PubMed:15489334"
FT                   /id="VSP_035235"
SQ   SEQUENCE   430 AA;  46170 MW;  297363BEB0E73F22 CRC64;
     MAVLARQLQR LLWTACKKKE REKEGREEEE EEEAGRRAPE GPRSLLTAPR RAQRPHGGAE
     ASGGLRFGAS AAQGWRARME DAHCTWLSLP GLPPGWALFA VLDGHGGARA ARFGARHLPG
     HVLQELGPEP SEPEGVREAL RRAFLSADER LRSLWPRVET GGCTAVVLLV SPRFLYLAHC
     GDSRAVLSRA GAVAFSTEDH RPLRPRERER IHAAGGTIRR RRVEGSLAVS RALGDFTYKE
     APGRPPELQL VSAEPEVAAL ARQAEDEFML LASDGVWDTV SGAALAGLVA SRLRLGLAPE
     LLCAQLLDTC LCKGSLDNMT CILVCFPGAP RPSEEAIRRE LALDAALGCR IAELCASAQK
     PPSLNTVFRT LASEDIPDLP PGGGLDCKAT VIAEVYSQIC QVSEECGEKG QDGAGKSNPT
     HLGSALDMEA
//