ID   PO5F2_HUMAN             Reviewed;         328 AA.
AC   Q8N7G0; Q15169; Q6MZL7; Q8N748;
DT   01-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   27-MAR-2024, entry version 151.
DE   RecName: Full=POU domain, class 5, transcription factor 2;
DE   AltName: Full=Sperm 1 POU domain transcription factor;
DE            Short=SPRM-1;
GN   Name=POU5F2; Synonyms=SPRM1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 148-328.
RC   TISSUE=Uterus;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 168-254, AND TISSUE SPECIFICITY.
RC   TISSUE=Skeletal muscle;
RX   PubMed=7908264; DOI=10.1111/j.1432-1033.1994.tb18676.x;
RA   Wey E., Lyons G.E., Schaefer B.W.;
RT   "A human POU domain gene, mPOU, is expressed in developing brain and
RT   specific adult tissues.";
RL   Eur. J. Biochem. 220:753-762(1994).
CC   -!- FUNCTION: Transcription factor that binds preferentially to the octamer
CC       motif (5'-ATGTTAAT-3'). May exert a regulatory function in meiotic
CC       events that are required for terminal differentiation of male germ cell
CC       (By similarity). {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q8N7G0; Q3LI66: KRTAP6-2; NbExp=3; IntAct=EBI-12849044, EBI-11962084;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC       ECO:0000255|PROSITE-ProRule:PRU00530}.
CC   -!- TISSUE SPECIFICITY: Expressed in skeletal and cardiac muscles, brain,
CC       heart and lung. Little or no detectable expression found in pancreas,
CC       kidney, liver or placenta. {ECO:0000269|PubMed:7908264}.
CC   -!- SIMILARITY: Belongs to the POU transcription factor family. Class-5
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AK098546; BAC05328.1; -; mRNA.
DR   EMBL; BC029532; AAH29532.2; -; mRNA.
DR   EMBL; BX641019; CAE46012.1; -; mRNA.
DR   EMBL; Z21965; CAA79976.1; -; mRNA.
DR   CCDS; CCDS59489.1; -.
DR   RefSeq; NP_694948.1; NM_153216.1.
DR   AlphaFoldDB; Q8N7G0; -.
DR   SMR; Q8N7G0; -.
DR   BioGRID; 126388; 2.
DR   IntAct; Q8N7G0; 1.
DR   STRING; 9606.ENSP00000489796; -.
DR   iPTMnet; Q8N7G0; -.
DR   PhosphoSitePlus; Q8N7G0; -.
DR   BioMuta; POU5F2; -.
DR   DMDM; 28201897; -.
DR   EPD; Q8N7G0; -.
DR   MassIVE; Q8N7G0; -.
DR   PaxDb; 9606-ENSP00000464890; -.
DR   PeptideAtlas; Q8N7G0; -.
DR   Antibodypedia; 64781; 155 antibodies from 21 providers.
DR   DNASU; 134187; -.
DR   Ensembl; ENST00000606183.4; ENSP00000489796.1; ENSG00000248483.7.
DR   GeneID; 134187; -.
DR   KEGG; hsa:134187; -.
DR   MANE-Select; ENST00000606183.4; ENSP00000489796.1; NM_153216.2; NP_694948.1.
DR   UCSC; uc003kkl.2; human.
DR   AGR; HGNC:26367; -.
DR   DisGeNET; 134187; -.
DR   GeneCards; POU5F2; -.
DR   HGNC; HGNC:26367; POU5F2.
DR   HPA; ENSG00000248483; Tissue enhanced (retina, testis).
DR   neXtProt; NX_Q8N7G0; -.
DR   OpenTargets; ENSG00000248483; -.
DR   PharmGKB; PA162399970; -.
DR   VEuPathDB; HostDB:ENSG00000248483; -.
DR   eggNOG; KOG3802; Eukaryota.
DR   GeneTree; ENSGT00940000164293; -.
DR   HOGENOM; CLU_066243_0_0_1; -.
DR   InParanoid; Q8N7G0; -.
DR   OMA; GPYIALR; -.
DR   OrthoDB; 4250502at2759; -.
DR   PhylomeDB; Q8N7G0; -.
DR   PathwayCommons; Q8N7G0; -.
DR   BioGRID-ORCS; 134187; 16 hits in 1146 CRISPR screens.
DR   GenomeRNAi; 134187; -.
DR   Pharos; Q8N7G0; Tdark.
DR   PRO; PR:Q8N7G0; -.
DR   Proteomes; UP000005640; Chromosome 5.
DR   RNAct; Q8N7G0; Protein.
DR   Bgee; ENSG00000248483; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 16 other cell types or tissues.
DR   GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   Gene3D; 1.10.260.40; lambda repressor-like DNA-binding domains; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR013847; POU.
DR   InterPro; IPR000327; POU_dom.
DR   PANTHER; PTHR11636; POU DOMAIN; 1.
DR   PANTHER; PTHR11636:SF14; POU DOMAIN, CLASS 5, TRANSCRIPTION FACTOR 2; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00157; Pou; 1.
DR   PRINTS; PR00028; POUDOMAIN.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00352; POU; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   SUPFAM; SSF47413; lambda repressor-like DNA-binding domains; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00035; POU_1; 1.
DR   PROSITE; PS00465; POU_2; 1.
DR   PROSITE; PS51179; POU_3; 1.
DR   Genevisible; Q8N7G0; HS.
PE   1: Evidence at protein level;
KW   DNA-binding; Homeobox; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..328
FT                   /note="POU domain, class 5, transcription factor 2"
FT                   /id="PRO_0000100756"
FT   DOMAIN          118..192
FT                   /note="POU-specific"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT   DNA_BIND        210..269
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        2
FT                   /note="A -> D (in Ref. 2; AAH29532)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        229
FT                   /note="F -> L (in Ref. 3; CAE46012)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   328 AA;  36051 MW;  DD90D7153E69A3ED CRC64;
     MAGHRPSNHF CPLPGSGGGG PRGPMPLRVD TLTWLSTQAA PGRVMVWPAV RPGICPGPDV
     WRIPLGPLPH EFRGWIAPCR PRLGASEAGD WLRRPSEGAL PGPYIALRSI PKLPPPEDIS
     GILKELQQLA KELRQKRLSL GYSQADVGIA VGALFGKVLS QTTICRFEAQ QLSVANMWKL
     RPLLKKWLKE VEAENLLGLC KMEMILQQSG KWRRASRERR IGNSLEKFFQ RCPKPTPQQI
     SHIAGCLQLQ KDVVRVWFYN RSKMGSRPTN DASPREIVGT AGPPCPGAPV CFHLGLGLPV
     DIPHYTRLYS AGVAHSSAPA TTLGLLRF
//