Q8N720 (ZN655_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 655

Alternative name(s):
Vav-interacting Krueppel-like protein

Gene names

Name:	ZNF655
Synonyms:	VIK

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in transcriptional regulation.
Subunit structure	Interacts with VAV1 and CDK4. Ref.1
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 6 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G1 phase Inferred from expression pattern Ref.1. Source: UniProtKB regulation of mitotic cell cycle Inferred from expression pattern Ref.1. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay Ref.1. Source: UniProtKB nucleolus Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction Ref.1. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDK4	P11802	3	EBI-625509,EBI-295644
VAV1	P15498	5	EBI-625509,EBI-625518

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N720-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N720-2)

The sequence of this isoform differs from the canonical sequence as follows:
     46-181: DGETREENKL...SGKHEHLNLT → APPVPQVPAL...PCSETPFPRL
     182-491: Missing.

Note: Phosphorylated on Ser-60.

Isoform 3 (identifier: Q8N720-3)

The sequence of this isoform differs from the canonical sequence as follows:
     45-45: G → GGFPISKPDGISQLEQDLQVFDLETKTREVLRDDCS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Zinc finger protein 655		PRO_0000047700
Regions
	Zinc finger	212 – 234	23	C2H2-type 1
	Zinc finger	240 – 262	23	C2H2-type 2
	Zinc finger	303 – 325	23	C2H2-type 3
	Zinc finger	330 – 353	24	C2H2-type 4
	Zinc finger	380 – 402	23	C2H2-type 5
	Zinc finger	408 – 430	23	C2H2-type 6
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.9
	Modified residue	12	1	Phosphoserine Ref.7 Ref.9 Ref.10
Natural variations
	Alternative sequence	45	1	G → GGFPISKPDGISQLEQDLQV FDLETKTREVLRDDCS in isoform 3.		VSP_041157
	Alternative sequence	46 – 181	136	DGETR…HLNLT → APPVPQVPALPREGSPGDQA AALLTARYQEFVTFEDVAVH LTREEWGYLDPVQRDLYREV MLENYGNVVSLGILLRLPTT RIHSVNSCPALSHTQASAFS GETLAVLTAGISKRWPKYRL PIDIARPCSETPFPRL in isoform 2.		VSP_036030
	Alternative sequence	182 – 491	310	Missing in isoform 2.		VSP_036031
	Natural variant	52	1	E → D. Ref.6 Corresponds to variant rs17853754 [ dbSNP | Ensembl ].		VAR_028165
Experimental info
	Sequence conflict	178	1	L → S in BAH14395. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 17, 2006. Version 3. Checksum: 04FD791D769C3A80	FASTA	491	57,407
	10 20 30 40 50 60 MEEIPAQEAA GSPRVQFQSL ETQSECLSPE PQFVQDTDME QGLTGDGETR EENKLLIPKQ 70 80 90 100 110 120 KISEEVHSYK VRVGRLKHDI TQVPETREVY KSEDRLERLQ EILRKFLYLE REFRQITISK 130 140 150 160 170 180 ETFTSEKNNE CHEPEKSFSL DSTIDADQRV LRIQNTDDND KYDMSFNQNS ASGKHEHLNL 190 200 210 220 230 240 TEDFQSSECK ESLMDLSHLN KWESIPNTEK SYKCDVCGKI FHQSSALTRH QRIHTREKPY 250 260 270 280 290 300 KCKECEKSFS QSSSLSRHKR IHTREKPYKC EASDKSCEAS DKSCSPSSGI IQHKKIHTRA 310 320 330 340 350 360 KSYKCSSCER VFSRSVHLTQ HQKIHKEMPC KCTVCGSDFC HTSYLLEHQR VHHEEKAYEY 370 380 390 400 410 420 DEYGLAYIKQ QGIHFREKPY TCSECGKDFR LNSHLIQHQR IHTGEKAHEC NECGKAFSQT 430 440 450 460 470 480 SCLIQHHKMH RKEKSYECNE YEGSFSHSSD LILQQEVLTR QKAFDCDVWE KNSSQRAHLV 490 QHQSIHTKEN S
	Isoform 2 [UniParc]. Checksum: 5279EA5A29623353	FASTA	181	20,005
	10 20 30 40 50 60 MEEIPAQEAA GSPRVQFQSL ETQSECLSPE PQFVQDTDME QGLTGAPPVP QVPALPREGS 70 80 90 100 110 120 PGDQAAALLT ARYQEFVTFE DVAVHLTREE WGYLDPVQRD LYREVMLENY GNVVSLGILL 130 140 150 160 170 180 RLPTTRIHSV NSCPALSHTQ ASAFSGETLA VLTAGISKRW PKYRLPIDIA RPCSETPFPR L
	Isoform 3 [UniParc]. Checksum: 10FD9EB53A793034	FASTA	526	61,369
	10 20 30 40 50 60 MEEIPAQEAA GSPRVQFQSL ETQSECLSPE PQFVQDTDME QGLTGGFPIS KPDGISQLEQ 70 80 90 100 110 120 DLQVFDLETK TREVLRDDCS DGETREENKL LIPKQKISEE VHSYKVRVGR LKHDITQVPE 130 140 150 160 170 180 TREVYKSEDR LERLQEILRK FLYLEREFRQ ITISKETFTS EKNNECHEPE KSFSLDSTID 190 200 210 220 230 240 ADQRVLRIQN TDDNDKYDMS FNQNSASGKH EHLNLTEDFQ SSECKESLMD LSHLNKWESI 250 260 270 280 290 300 PNTEKSYKCD VCGKIFHQSS ALTRHQRIHT REKPYKCKEC EKSFSQSSSL SRHKRIHTRE 310 320 330 340 350 360 KPYKCEASDK SCEASDKSCS PSSGIIQHKK IHTRAKSYKC SSCERVFSRS VHLTQHQKIH 370 380 390 400 410 420 KEMPCKCTVC GSDFCHTSYL LEHQRVHHEE KAYEYDEYGL AYIKQQGIHF REKPYTCSEC 430 440 450 460 470 480 GKDFRLNSHL IQHQRIHTGE KAHECNECGK AFSQTSCLIQ HHKMHRKEKS YECNEYEGSF 490 500 510 520 SHSSDLILQQ EVLTRQKAFD CDVWEKNSSQ RAHLVQHQSI HTKENS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of VIK-1: a new Vav-interacting Kruppel-like protein." Houlard M., Romero-Portillo F., Germani A., Depaux A., Regnier-Ricard F., Gisselbrecht S., Varin-Blank N. Oncogene 24:28-38(2005) [PubMed: 15558030] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH VAV1 AND CDK4.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Brain, Trachea and Uterus.
[3]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed: 12690205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ASP-52. Tissue: Brain, Cervix, Pancreas, Placenta and Skin.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60 (ISOFORM 2), MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60 (ISOFORM 2), MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[10]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY099353 mRNA. Translation: AAM33786.1. AK304785 mRNA. Translation: BAG65536.1. AK314593 mRNA. Translation: BAG37165.1. AK316024 mRNA. Translation: BAH14395.1. AC005020 Genomic DNA. Translation: AAS02017.1. CH236956 Genomic DNA. Translation: EAL23871.1. CH471091 Genomic DNA. Translation: EAW76650.1. CH236956 Genomic DNA. Translation: EAL23870.1. CH471091 Genomic DNA. Translation: EAW76651.1. BC000823 mRNA. Translation: AAH00823.1. BC004288 mRNA. Translation: AAH04288.1. BC007378 mRNA. Translation: AAH07378.1. BC011816 mRNA. Translation: AAH11816.1. BC024770 mRNA. Translation: AAH24770.1. BC037407 mRNA. Translation: AAH37407.1.
IPI	IPI00031606. IPI00292660. IPI00925360.
RefSeq	NP_001009960.1. NM_001009960.1. NP_001077425.1. NM_001083956.1. NP_001078835.1. NM_001085366.1. NP_001078836.1. NM_001085367.1. NP_001078837.1. NM_001085368.1. NP_076966.1. NM_024061.3. NP_612503.1. NM_138494.2.
UniGene	Hs.599798.
3D structure databases
ProteinModelPortal	Q8N720.
SMR	Q8N720. Positions 116-281, 346-490.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-34108N.
IntAct	Q8N720. 11 interactions.
MINT	MINT-1211608.
STRING	Q8N720.
PTM databases
PhosphoSite	Q8N720.
Polymorphism databases
DMDM	116242863.
Proteomic databases
PeptideAtlas	Q8N720.
PRIDE	Q8N720.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000252713; ENSP00000252713; ENSG00000197343. ENST00000394163; ENSP00000377718; ENSG00000197343.
GeneID	79027.
KEGG	hsa:79027.
UCSC	uc003urh.1. human.
Organism-specific databases
CTD	79027.
GeneCards	GC07P099156.
HGNC	HGNC:30899. ZNF655.
HPA	HPA029534.
neXtProt	NX_Q8N720.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000021552.
HOVERGEN	HBG018163.
InParanoid	Q8N720.
OrthoDB	EOG4D7Z5D.
PhylomeDB	Q8N720.
Gene expression databases
ArrayExpress	Q8N720.
Bgee	Q8N720.
CleanEx	HS_ZNF655.
Genevestigator	Q8N720.
GermOnline	ENSG00000197343. Homo sapiens.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 9 hits.
Pfam	PF00096. zf-C2H2. 5 hits. [Graphical view]
SMART	SM00355. ZnF_C2H2. 7 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 6 hits. PS50157. ZINC_FINGER_C2H2_2. 6 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	67721.

Entry information

Entry name

ZN655_HUMAN

Accession

Primary (citable) accession number: Q8N720
Secondary accession number(s): A4D291 , B4E3M4, B7Z9Q9, D6W5T4, Q8IV00, Q8TA89, Q96EZ3, Q9BQ85

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.