Reviewed, UniProtKB/Swiss-Prot Q8N720 (ZN655_HUMAN)

Names and origin

Protein names

Recommended name:
    Zinc finger protein 655
Alternative name(s):
    Vav-interacting Krueppel-like protein

Gene names

Name:	ZNF655
Synonyms:	VIK

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in transcriptional regulation.
Subunit structure	Interacts with VAV1 and CDK4. Ref.1
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 6 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.1 Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDK4	P11802	2	EBI-625509,EBI-295644
VAV1	P15498	4	EBI-625509,EBI-625518

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N720-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N720-2)

The sequence of this isoform differs from the canonical sequence as follows:
     46-181: DGETREENKL...SGKHEHLNLT → APPVPQVPAL...PCSETPFPRL
     182-491: Missing.

Note: Phosphorylated on Ser-60.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Zinc finger protein 655		PRO_0000047700
Regions
	Zinc finger	212 – 234	23	C2H2-type 1
	Zinc finger	240 – 262	23	C2H2-type 2
	Zinc finger	303 – 325	23	C2H2-type 3
	Zinc finger	330 – 353	24	C2H2-type 4
	Zinc finger	380 – 402	23	C2H2-type 5
	Zinc finger	408 – 430	23	C2H2-type 6
Amino acid modifications
	Modified residue	12	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	46 – 181	136	DGETR…HLNLT → APPVPQVPALPREGSPGDQA AALLTARYQEFVTFEDVAVH LTREEWGYLDPVQRDLYREV MLENYGNVVSLGILLRLPTT RIHSVNSCPALSHTQASAFS GETLAVLTAGISKRWPKYRL PIDIARPCSETPFPRL in isoform 2.		VSP_036030
	Alternative sequence	182 – 491	310	Missing in isoform 2.		VSP_036031
	Natural variant	52	1	E → D Ref.5		VAR_028165

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 17, 2006. Version 3. Checksum: 04FD791D769C3A80	FASTA	491	57,407
	10 20 30 40 50 60 MEEIPAQEAA GSPRVQFQSL ETQSECLSPE PQFVQDTDME QGLTGDGETR EENKLLIPKQ 70 80 90 100 110 120 KISEEVHSYK VRVGRLKHDI TQVPETREVY KSEDRLERLQ EILRKFLYLE REFRQITISK 130 140 150 160 170 180 ETFTSEKNNE CHEPEKSFSL DSTIDADQRV LRIQNTDDND KYDMSFNQNS ASGKHEHLNL 190 200 210 220 230 240 TEDFQSSECK ESLMDLSHLN KWESIPNTEK SYKCDVCGKI FHQSSALTRH QRIHTREKPY 250 260 270 280 290 300 KCKECEKSFS QSSSLSRHKR IHTREKPYKC EASDKSCEAS DKSCSPSSGI IQHKKIHTRA 310 320 330 340 350 360 KSYKCSSCER VFSRSVHLTQ HQKIHKEMPC KCTVCGSDFC HTSYLLEHQR VHHEEKAYEY 370 380 390 400 410 420 DEYGLAYIKQ QGIHFREKPY TCSECGKDFR LNSHLIQHQR IHTGEKAHEC NECGKAFSQT 430 440 450 460 470 480 SCLIQHHKMH RKEKSYECNE YEGSFSHSSD LILQQEVLTR QKAFDCDVWE KNSSQRAHLV 490 QHQSIHTKEN S
	Isoform 2. Checksum: 5279EA5A29623353	FASTA	181	20,005
	10 20 30 40 50 60 MEEIPAQEAA GSPRVQFQSL ETQSECLSPE PQFVQDTDME QGLTGAPPVP QVPALPREGS 70 80 90 100 110 120 PGDQAAALLT ARYQEFVTFE DVAVHLTREE WGYLDPVQRD LYREVMLENY GNVVSLGILL 130 140 150 160 170 180 RLPTTRIHSV NSCPALSHTQ ASAFSGETLA VLTAGISKRW PKYRLPIDIA RPCSETPFPR L

References

[1]	"Characterization of VIK-1: a new Vav-interacting Kruppel-like protein." Houlard M., Romero-Portillo F., Germani A., Depaux A., Regnier-Ricard F., Gisselbrecht S., Varin-Blank N. Oncogene 24:28-38(2005) [PubMed: 15558030] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH VAV1 AND CDK4.
[2]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed: 12690205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ASP-52. Tissue: Brain, Cervix, Pancreas, Placenta and Skin.
[6]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60 (ISOFORM 2), MASS SPECTROMETRY.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60 (ISOFORM 2), MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY099353 mRNA. Translation: AAM33786.1. AC005020 Genomic DNA. Translation: AAS02017.1. CH236956 Genomic DNA. Translation: EAL23871.1. CH471091 Genomic DNA. Translation: EAW76650.1. BC000823 mRNA. Translation: AAH00823.1. BC004288 mRNA. Translation: AAH04288.1. BC007378 mRNA. Translation: AAH07378.1. BC011816 mRNA. Translation: AAH11816.1. BC024770 mRNA. Translation: AAH24770.1. BC037407 mRNA. Translation: AAH37407.1.
IPI	IPI00031606. IPI00292660.
RefSeq	NP_001009960.1. NP_001078835.1. NP_001078836.1. NP_076966.1. NP_612503.1.
UniGene	Hs.696221
3D structure databases
HSSP	HSSP built from PDB template 1ZNF based on UniProtKB P08045.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N720. 8 interactions.
Proteomic databases
PeptideAtlas	Q8N720.
PRIDE	Q8N720.
Genome annotation databases
Ensembl	ENSG00000197343. Homo sapiens. [Contig view]
GeneID	79027.
Organism-specific databases
GeneCards	GC07P098993.
HGNC	HGNC:30899. ZNF655.
PharmGKB	PA134883537.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8N720.
HOVERGEN	Q8N720.
OMA	Q8N720. RIHTKEN.
Gene expression databases
ArrayExpress	Q8N720.
Bgee	Q8N720.
CleanEx	HS_ZNF655.
GermOnline	ENSG00000197343. Homo sapiens.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 4 hits.
Pfam	PF00096. zf-C2H2. 6 hits. [Graphical view]
ProDom	PD000003. Znf_C2H2. 5 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00355. ZnF_C2H2. 7 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 6 hits. PS50157. ZINC_FINGER_C2H2_2. 6 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	67721.

Entry information

Entry name

ZN655_HUMAN

Accession

Primary (citable) accession number: Q8N720
Secondary accession number(s): Q8IV00 , Q8TA89, Q96EZ3, Q9BQ85

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 55 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)