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Protein

CUGBP Elav-like family member 5

Gene

CELF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CUGBP Elav-like family member 5
Short name:
CELF-5
Alternative name(s):
Bruno-like protein 5
CUG-BP- and ETR-3-like factor 5
RNA-binding protein BRUNOL-5
Gene namesi
Name:CELF5
Synonyms:BRUNOL5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:14058. CELF5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25429.

Polymorphism and mutation databases

BioMutaiCELF5.
DMDMi74762534.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485CUGBP Elav-like family member 5PRO_0000295227Add
BLAST

Proteomic databases

PaxDbiQ8N6W0.
PRIDEiQ8N6W0.

PTM databases

PhosphoSiteiQ8N6W0.

Expressioni

Tissue specificityi

Expressed in brain.1 Publication

Gene expression databases

BgeeiQ8N6W0.
CleanExiHS_BRUNOL5.
ExpressionAtlasiQ8N6W0. baseline and differential.
GenevisibleiQ8N6W0. HS.

Organism-specific databases

HPAiHPA042012.
HPA060336.

Interactioni

Protein-protein interaction databases

BioGridi121954. 13 interactions.
STRINGi9606.ENSP00000292672.

Structurei

Secondary structure

1
485
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi135 – 1406Combined sources
Helixi147 – 1548Combined sources
Turni155 – 1573Combined sources
Beta strandi160 – 1678Combined sources
Beta strandi169 – 1713Combined sources
Beta strandi173 – 18311Combined sources
Helixi184 – 19411Combined sources
Beta strandi208 – 2125Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DNHNMR-A126-217[»]
ProteinModelPortaliQ8N6W0.
SMRiQ8N6W0. Positions 25-217, 396-476.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8N6W0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 12682RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini134 – 21481RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini400 – 47879RRM 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CELF/BRUNOL family.Curated
Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0146. Eukaryota.
ENOG410XNTW. LUCA.
GeneTreeiENSGT00560000076837.
HOVERGENiHBG107646.
InParanoidiQ8N6W0.
KOiK13207.
OMAiAPIANGF.
OrthoDBiEOG7DVDBR.
PhylomeDBiQ8N6W0.
TreeFamiTF314924.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N6W0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARLTESEAR RQQQQLLQPR PSPVGSSGPE PPGGQPDGMK DLDAIKLFVG
60 70 80 90 100
QIPRHLDEKD LKPLFEQFGR IYELTVLKDP YTGMHKGCAF LTYCARDSAI
110 120 130 140 150
KAQTALHEQK TLPGMARPIQ VKPADSESRG GRDRKLFVGM LNKQQSEEDV
160 170 180 190 200
LRLFQPFGVI DECTVLRGPD GSSKGCAFVK FSSHTEAQAA IHALHGSQTM
210 220 230 240 250
PGASSSLVVK FADTDKERTL RRMQQMVGQL GILTPSLTLP FSPYSAYAQA
260 270 280 290 300
LMQQQTTVLS TSGSYLSPGV AFSPCHIQQI GAVSLNGLPA TPIAPASGLH
310 320 330 340 350
SPPLLGTTAV PGLVAPITNG FAGVVPFPGG HPALETVYAN GLVPYPAQSP
360 370 380 390 400
TVAETLHPAF SGVQQYTAMY PTAAITPIAH SVPQPPPLLQ QQQREGPEGC
410 420 430 440 450
NLFIYHLPQE FGDTELTQMF LPFGNIISSK VFMDRATNQS KCFGFVSFDN
460 470 480
PASAQAAIQA MNGFQIGMKR LKVQLKRPKD PGHPY
Length:485
Mass (Da):52,355
Last modified:October 1, 2002 - v1
Checksum:iAB805B4971619E05
GO
Isoform 2 (identifier: Q8N6W0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     298-322: Missing.
     397-434: PEGCNLFIYH...NIISSKVFMD → VWRHGADADV...PEQVFRLREL
     435-485: Missing.

Show »
Length:409
Mass (Da):44,479
Checksum:i6065BB4FE2DFAE78
GO

Sequence cautioni

The sequence AAC27666.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAK07476.1 differs from that shown. Reason: Erroneous termination at position 482. Translated as Gly.Curated
The sequence BAD92304.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti293 – 2931I → V in AAK07476 (PubMed:11158314).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti65 – 651F → L.1 Publication
Corresponds to variant rs17854481 [ dbSNP | Ensembl ].
VAR_033264

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei298 – 32225Missing in isoform 2. 1 PublicationVSP_026844Add
BLAST
Alternative sequencei397 – 43438PEGCN…KVFMD → VWRHGADADVPTLRQYHFLQ GVYGSSYQPEQVFRLREL in isoform 2. 1 PublicationVSP_026845Add
BLAST
Alternative sequencei435 – 48551Missing in isoform 2. 1 PublicationVSP_026846Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB209067 mRNA. Translation: BAD92304.1. Different initiation.
AC005331 Genomic DNA. Translation: AAC27666.1. Different initiation.
AC006505 Genomic DNA. No translation available.
AC010649 Genomic DNA. No translation available.
AC123911 Genomic DNA. No translation available.
CH471139 Genomic DNA. Translation: EAW69327.1.
CH471139 Genomic DNA. Translation: EAW69328.1.
BC028101 mRNA. Translation: AAH28101.1.
AF329266 mRNA. Translation: AAK07476.1. Sequence problems.
AF248649 mRNA. Translation: AAF86231.1.
CCDSiCCDS12106.1. [Q8N6W0-1]
CCDS54197.1. [Q8N6W0-2]
RefSeqiNP_001166144.1. NM_001172673.1. [Q8N6W0-2]
NP_068757.2. NM_021938.3. [Q8N6W0-1]
XP_006722895.1. XM_006722832.1. [Q8N6W0-1]
UniGeneiHs.655747.

Genome annotation databases

EnsembliENST00000292672; ENSP00000292672; ENSG00000161082. [Q8N6W0-1]
ENST00000541430; ENSP00000443498; ENSG00000161082. [Q8N6W0-2]
GeneIDi60680.
KEGGihsa:60680.
UCSCiuc002lxm.4. human. [Q8N6W0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB209067 mRNA. Translation: BAD92304.1. Different initiation.
AC005331 Genomic DNA. Translation: AAC27666.1. Different initiation.
AC006505 Genomic DNA. No translation available.
AC010649 Genomic DNA. No translation available.
AC123911 Genomic DNA. No translation available.
CH471139 Genomic DNA. Translation: EAW69327.1.
CH471139 Genomic DNA. Translation: EAW69328.1.
BC028101 mRNA. Translation: AAH28101.1.
AF329266 mRNA. Translation: AAK07476.1. Sequence problems.
AF248649 mRNA. Translation: AAF86231.1.
CCDSiCCDS12106.1. [Q8N6W0-1]
CCDS54197.1. [Q8N6W0-2]
RefSeqiNP_001166144.1. NM_001172673.1. [Q8N6W0-2]
NP_068757.2. NM_021938.3. [Q8N6W0-1]
XP_006722895.1. XM_006722832.1. [Q8N6W0-1]
UniGeneiHs.655747.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DNHNMR-A126-217[»]
ProteinModelPortaliQ8N6W0.
SMRiQ8N6W0. Positions 25-217, 396-476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121954. 13 interactions.
STRINGi9606.ENSP00000292672.

PTM databases

PhosphoSiteiQ8N6W0.

Polymorphism and mutation databases

BioMutaiCELF5.
DMDMi74762534.

Proteomic databases

PaxDbiQ8N6W0.
PRIDEiQ8N6W0.

Protocols and materials databases

DNASUi60680.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292672; ENSP00000292672; ENSG00000161082. [Q8N6W0-1]
ENST00000541430; ENSP00000443498; ENSG00000161082. [Q8N6W0-2]
GeneIDi60680.
KEGGihsa:60680.
UCSCiuc002lxm.4. human. [Q8N6W0-1]

Organism-specific databases

CTDi60680.
GeneCardsiCELF5.
HGNCiHGNC:14058. CELF5.
HPAiHPA042012.
HPA060336.
MIMi612680. gene.
neXtProtiNX_Q8N6W0.
PharmGKBiPA25429.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0146. Eukaryota.
ENOG410XNTW. LUCA.
GeneTreeiENSGT00560000076837.
HOVERGENiHBG107646.
InParanoidiQ8N6W0.
KOiK13207.
OMAiAPIANGF.
OrthoDBiEOG7DVDBR.
PhylomeDBiQ8N6W0.
TreeFamiTF314924.

Miscellaneous databases

EvolutionaryTraceiQ8N6W0.
GenomeRNAii60680.
NextBioi65500.
PROiQ8N6W0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8N6W0.
CleanExiHS_BRUNOL5.
ExpressionAtlasiQ8N6W0. baseline and differential.
GenevisibleiQ8N6W0. HS.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LEU-65.
    Tissue: Brain.
  5. "The CELF family of RNA binding proteins is implicated in cell-specific and developmentally regulated alternative splicing."
    Ladd A.N., Charlet-B N., Cooper T.A.
    Mol. Cell. Biol. 21:1285-1296(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-482 (ISOFORM 1), FUNCTION, RNA-BINDING, TISSUE SPECIFICITY.
    Tissue: Brain.
  6. "A family of human RNA-binding proteins related to the Drosophila Bruno translational regulator."
    Good P.J., Chen Q., Warner S.J., Herring D.C.
    J. Biol. Chem. 275:28583-28592(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 396-478 (ISOFORM 1).
    Tissue: Brain.
  7. "Solution structure of RNA-binding domain in bruno-like 5 RNA-binding protein."
    RIKEN structural genomics initiative (RSGI)
    Submitted (OCT-2006) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 126-217.

Entry informationi

Entry nameiCELF5_HUMAN
AccessioniPrimary (citable) accession number: Q8N6W0
Secondary accession number(s): D6W614
, O75253, Q59GP2, Q86VW6, Q9BZC0, Q9NR86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: October 1, 2002
Last modified: March 16, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.