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Protein

ADP-ribosylation factor GTPase-activating protein 1

Gene

ARFGAP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri22 – 45C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • GTPase activator activity Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000101199-MONOMER.
ReactomeiR-HSA-381038. XBP1(S) activates chaperone genes.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-8856828. Clathrin-mediated endocytosis.
SIGNORiQ8N6T3.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor GTPase-activating protein 1
Short name:
ARF GAP 1
Alternative name(s):
ADP-ribosylation factor 1 GTPase-activating protein
Short name:
ARF1 GAP
ARF1-directed GTPase-activating protein
Gene namesi
Name:ARFGAP1
Synonyms:ARF1GAP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:15852. ARFGAP1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55738.
OpenTargetsiENSG00000101199.
PharmGKBiPA164741246.

Polymorphism and mutation databases

DMDMi27923731.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741901 – 406ADP-ribosylation factor GTPase-activating protein 1Add BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei135PhosphothreonineCombined sources1
Modified residuei150PhosphoserineCombined sources1
Modified residuei189PhosphothreonineCombined sources1
Modified residuei231N6-acetyllysineCombined sources1
Modified residuei246PhosphoserineCombined sources1
Modified residuei304PhosphoserineCombined sources1
Modified residuei343PhosphoserineCombined sources1
Modified residuei346PhosphoserineBy similarity1
Modified residuei348PhosphoserineBy similarity1
Modified residuei350PhosphothreonineCombined sources1
Modified residuei361PhosphoserineCombined sources1
Modified residuei363PhosphoserineBy similarity1
Modified residuei378PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8N6T3.
MaxQBiQ8N6T3.
PaxDbiQ8N6T3.
PeptideAtlasiQ8N6T3.
PRIDEiQ8N6T3.

PTM databases

iPTMnetiQ8N6T3.
PhosphoSitePlusiQ8N6T3.

Expressioni

Gene expression databases

BgeeiENSG00000101199.
CleanExiHS_ARFGAP1.
ExpressionAtlasiQ8N6T3. baseline and differential.
GenevisibleiQ8N6T3. HS.

Organism-specific databases

HPAiHPA051019.
HPA056273.

Interactioni

Subunit structurei

Interacts with ARF1. Interacts with the COPI coat proteins, KDELR1 and TMED2. The interaction with TMED2 inhibits the GAP activity (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
FOSQ6FG413EBI-716933,EBI-10198738
GABARAPL2P605203EBI-716933,EBI-720116
GRNP287993EBI-716933,EBI-747754
KRTAP10-9P604113EBI-716933,EBI-10172052
KRTAP3-2Q9BYR73EBI-716933,EBI-751260
KRTAP9-2Q9BYQ43EBI-716933,EBI-1044640
LRRK2Q5S0076EBI-6288865,EBI-5323863
TRIM23P364063EBI-716933,EBI-740098

Protein-protein interaction databases

BioGridi120856. 65 interactors.
IntActiQ8N6T3. 28 interactors.
MINTiMINT-1409968.
STRINGi9606.ENSP00000314615.

Structurei

Secondary structure

1406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 14Combined sources11
Turni17 – 20Combined sources4
Turni23 – 25Combined sources3
Beta strandi32 – 34Combined sources3
Turni35 – 38Combined sources4
Beta strandi39 – 41Combined sources3
Helixi43 – 52Combined sources10
Turni54 – 56Combined sources3
Beta strandi59 – 62Combined sources4
Helixi69 – 77Combined sources9
Helixi80 – 88Combined sources9
Helixi99 – 103Combined sources5
Helixi106 – 119Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DWDX-ray2.40A/B1-128[»]
3O47X-ray2.80A/B1-140[»]
ProteinModelPortaliQ8N6T3.
SMRiQ8N6T3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8N6T3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 124Arf-GAPPROSITE-ProRule annotationAdd BLAST118

Domaini

The region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes (By similarity).By similarity

Sequence similaritiesi

Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri22 – 45C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0704. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00860000133876.
HOGENOMiHOG000008494.
HOVERGENiHBG050562.
InParanoidiQ8N6T3.
KOiK12492.
OMAiDGWDNQN.
OrthoDBiEOG091G0DQ3.
PhylomeDBiQ8N6T3.
TreeFamiTF105931.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N6T3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPRTRKVL KEVRVQDENN VCFECGAFNP QWVSVTYGIW ICLECSGRHR
60 70 80 90 100
GLGVHLSFVR SVTMDKWKDI ELEKMKAGGN AKFREFLESQ EDYDPCWSLQ
110 120 130 140 150
EKYNSRAAAL FRDKVVALAE GREWSLESSP AQNWTPPQPR TLPSMVHRVS
160 170 180 190 200
GQPQSVTASS DKAFEDWLND DLGSYQGAQG NRYVGFGNTP PPQKKEDDFL
210 220 230 240 250
NNAMSSLYSG WSSFTTGASR FASAAKEGAT KFGSQASQKA SELGHSLNEN
260 270 280 290 300
VLKPAQEKVK EGKIFDDVSS GVSQLASKVQ GVGSKGWRDV TTFFSGKAEG
310 320 330 340 350
PLDSPSEGHS YQNSGLDHFQ NSNIDQSFWE TFGSAEPTKT RKSPSSDSWT
360 370 380 390 400
CADTSTERRS SDSWEVWGSA STNRNSNSDG GEGGEGTKKA VPPAVPTDDG

WDNQNW
Length:406
Mass (Da):44,668
Last modified:January 27, 2003 - v2
Checksum:iCAE41828DE660621
GO
Isoform 2 (identifier: Q8N6T3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     239-239: K → KFWGHKQQPEP
     279-280: Missing.

Note: No experimental confirmation available.
Show »
Length:414
Mass (Da):45,676
Checksum:iBD081C6CE6CF0A18
GO
Isoform 3 (identifier: Q8N6T3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     279-406: VQGVGSKGWR...TDDGWDNQNW → CQRRLCCHQS...AVWTTSKTAT

Show »
Length:403
Mass (Da):44,467
Checksum:i0C695741E21D0F6F
GO
Isoform 4 (identifier: Q8N6T3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVS → MLSSESSWSLRRITILAGPCRRSTTAEPRPSLGI
     35-147: Missing.

Note: No experimental confirmation available.
Show »
Length:293
Mass (Da):31,476
Checksum:iC0EEFC7461FF6CB3
GO
Isoform 5 (identifier: Q8N6T3-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLS → MRTT
     239-239: K → KFWGHKQQPEP
     279-280: Missing.

Show »
Length:361
Mass (Da):39,705
Checksum:iD34FF3C257C7E8FB
GO

Sequence cautioni

The sequence BAB55009 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB55113 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB70901 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti274Q → R in BAB55009 (PubMed:14702039).Curated1
Sequence conflicti358R → G in BAH12326 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_015187184V → M.Corresponds to variant rs2273499dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0557391 – 57MASPR…GVHLS → MRTT in isoform 5. 1 PublicationAdd BLAST57
Alternative sequenceiVSP_0553791 – 34MASPR…PQWVS → MLSSESSWSLRRITILAGPC RRSTTAEPRPSLGI in isoform 4. 1 PublicationAdd BLAST34
Alternative sequenceiVSP_05538035 – 147Missing in isoform 4. 1 PublicationAdd BLAST113
Alternative sequenceiVSP_000298239K → KFWGHKQQPEP in isoform 2 and isoform 5. 2 Publications1
Alternative sequenceiVSP_021818279 – 406VQGVG…DNQNW → CQRRLCCHQSHCSAGHLGRA FCPVSWHEALCGQTGREEQA SLLPPKHVVGALEVCARGCP RCHVPHTPGTAAEWPGRLCL SRESVVRDGGTSPPFFRGKQ RAPWTAPRRATVIRTAVWTT SKTAT in isoform 3. 1 PublicationAdd BLAST128
Alternative sequenceiVSP_000299279 – 280Missing in isoform 2 and isoform 5. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001629 mRNA. Translation: BAA91796.1.
AK027268 mRNA. Translation: BAB55009.1. Different initiation.
AK296351 mRNA. Translation: BAH12326.1.
AK027441 mRNA. Translation: BAB55113.1. Different initiation.
AK303454 mRNA. Translation: BAH13964.1.
AL121827 Genomic DNA. Translation: CAX12645.1.
CH471077 Genomic DNA. Translation: EAW75292.1.
CH471077 Genomic DNA. Translation: EAW75294.1.
BC000786 mRNA. Translation: AAH00786.1.
BC006085 mRNA. Translation: AAH06085.1.
BC011876 mRNA. Translation: AAH11876.1.
BC028233 mRNA. Translation: AAH28233.1.
AL137744 mRNA. Translation: CAB70901.1. Sequence problems.
AB015340 mRNA. Translation: BAA88117.1.
CCDSiCCDS13515.1. [Q8N6T3-1]
CCDS13516.1. [Q8N6T3-2]
CCDS63326.1. [Q8N6T3-3]
CCDS63327.1. [Q8N6T3-5]
CCDS63328.1. [Q8N6T3-4]
PIRiT46298.
RefSeqiNP_001268411.1. NM_001281482.1. [Q8N6T3-3]
NP_001268412.1. NM_001281483.1. [Q8N6T3-5]
NP_001268413.1. NM_001281484.1. [Q8N6T3-4]
NP_060679.1. NM_018209.3. [Q8N6T3-1]
NP_783202.1. NM_175609.2. [Q8N6T3-2]
UniGeneiHs.25584.

Genome annotation databases

EnsembliENST00000353546; ENSP00000314615; ENSG00000101199. [Q8N6T3-2]
ENST00000370275; ENSP00000359298; ENSG00000101199. [Q8N6T3-3]
ENST00000370283; ENSP00000359306; ENSG00000101199. [Q8N6T3-1]
ENST00000519273; ENSP00000443716; ENSG00000101199. [Q8N6T3-4]
ENST00000519604; ENSP00000430500; ENSG00000101199. [Q8N6T3-5]
GeneIDi55738.
KEGGihsa:55738.
UCSCiuc002yel.5. human. [Q8N6T3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001629 mRNA. Translation: BAA91796.1.
AK027268 mRNA. Translation: BAB55009.1. Different initiation.
AK296351 mRNA. Translation: BAH12326.1.
AK027441 mRNA. Translation: BAB55113.1. Different initiation.
AK303454 mRNA. Translation: BAH13964.1.
AL121827 Genomic DNA. Translation: CAX12645.1.
CH471077 Genomic DNA. Translation: EAW75292.1.
CH471077 Genomic DNA. Translation: EAW75294.1.
BC000786 mRNA. Translation: AAH00786.1.
BC006085 mRNA. Translation: AAH06085.1.
BC011876 mRNA. Translation: AAH11876.1.
BC028233 mRNA. Translation: AAH28233.1.
AL137744 mRNA. Translation: CAB70901.1. Sequence problems.
AB015340 mRNA. Translation: BAA88117.1.
CCDSiCCDS13515.1. [Q8N6T3-1]
CCDS13516.1. [Q8N6T3-2]
CCDS63326.1. [Q8N6T3-3]
CCDS63327.1. [Q8N6T3-5]
CCDS63328.1. [Q8N6T3-4]
PIRiT46298.
RefSeqiNP_001268411.1. NM_001281482.1. [Q8N6T3-3]
NP_001268412.1. NM_001281483.1. [Q8N6T3-5]
NP_001268413.1. NM_001281484.1. [Q8N6T3-4]
NP_060679.1. NM_018209.3. [Q8N6T3-1]
NP_783202.1. NM_175609.2. [Q8N6T3-2]
UniGeneiHs.25584.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DWDX-ray2.40A/B1-128[»]
3O47X-ray2.80A/B1-140[»]
ProteinModelPortaliQ8N6T3.
SMRiQ8N6T3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120856. 65 interactors.
IntActiQ8N6T3. 28 interactors.
MINTiMINT-1409968.
STRINGi9606.ENSP00000314615.

PTM databases

iPTMnetiQ8N6T3.
PhosphoSitePlusiQ8N6T3.

Polymorphism and mutation databases

DMDMi27923731.

Proteomic databases

EPDiQ8N6T3.
MaxQBiQ8N6T3.
PaxDbiQ8N6T3.
PeptideAtlasiQ8N6T3.
PRIDEiQ8N6T3.

Protocols and materials databases

DNASUi55738.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000353546; ENSP00000314615; ENSG00000101199. [Q8N6T3-2]
ENST00000370275; ENSP00000359298; ENSG00000101199. [Q8N6T3-3]
ENST00000370283; ENSP00000359306; ENSG00000101199. [Q8N6T3-1]
ENST00000519273; ENSP00000443716; ENSG00000101199. [Q8N6T3-4]
ENST00000519604; ENSP00000430500; ENSG00000101199. [Q8N6T3-5]
GeneIDi55738.
KEGGihsa:55738.
UCSCiuc002yel.5. human. [Q8N6T3-1]

Organism-specific databases

CTDi55738.
DisGeNETi55738.
GeneCardsiARFGAP1.
H-InvDBHIX0174713.
HGNCiHGNC:15852. ARFGAP1.
HPAiHPA051019.
HPA056273.
MIMi608377. gene.
neXtProtiNX_Q8N6T3.
OpenTargetsiENSG00000101199.
PharmGKBiPA164741246.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0704. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00860000133876.
HOGENOMiHOG000008494.
HOVERGENiHBG050562.
InParanoidiQ8N6T3.
KOiK12492.
OMAiDGWDNQN.
OrthoDBiEOG091G0DQ3.
PhylomeDBiQ8N6T3.
TreeFamiTF105931.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000101199-MONOMER.
ReactomeiR-HSA-381038. XBP1(S) activates chaperone genes.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-8856828. Clathrin-mediated endocytosis.
SIGNORiQ8N6T3.

Miscellaneous databases

ChiTaRSiARFGAP1. human.
EvolutionaryTraceiQ8N6T3.
GeneWikiiARFGAP1.
GenomeRNAii55738.
PROiQ8N6T3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101199.
CleanExiHS_ARFGAP1.
ExpressionAtlasiQ8N6T3. baseline and differential.
GenevisibleiQ8N6T3. HS.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARFG1_HUMAN
AccessioniPrimary (citable) accession number: Q8N6T3
Secondary accession number(s): B7Z3U0
, B7Z8H8, B7ZBI3, E1P5I9, E7EV62, Q6PK71, Q96KC4, Q96T02, Q9NSU3, Q9NVF6, Q9UIL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: January 27, 2003
Last modified: November 30, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.