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Q8N6Q3 (CD177_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CD177 antigen
Alternative name(s):
Human neutrophil alloantigen 2a
Short name=HNA-2a
NB1 glycoprotein
Short name=NB1 GP
Polycythemia rubra vera protein 1
Short name=PRV-1
CD_antigen=CD177
Gene names
Name:CD177
Synonyms:NB1, PRV1
ORF Names:UNQ595/PRO1181
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length437 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor Ref.8.

Tissue specificity

Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET). Ref.1 Ref.9

Induction

By CSF3 in resting granulocytes. Induced in patients with polycythemia vera (PV) and with essential thrombocythemia (ET). Ref.1 Ref.9

Post-translational modification

N-glycosylated. Ref.2 Ref.8

A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).

Polymorphism

There is a significant association between the variants Ala-3, Leu-251 and Thr-348 and a low expression of CD177 on neutrophils.

Sequence similarities

Contains 2 UPAR/Ly6 domains.

Mass spectrometry

Molecular mass is 50556 Da from positions 22 - 408. Determined by MALDI. Ref.2

Ontologies

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8N6Q3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8N6Q3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     210-248: TCHRGTTIMT...EVGQVCQETL → QLKPVGLLEE...WQKRAATGIF
     249-437: Missing.
Note: Associated with CD177-negative phenotype.
Isoform 3 (identifier: Q8N6Q3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     129-145: GSLRCPVCLSMEGCLEG → MMGAAEGPFGKAEQVDS
     146-437: Missing.
Note: Associated with CD177-negative phenotype.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Ref.7
Chain22 – 408387CD177 antigen
PRO_0000231643
Propeptide409 – 43729Removed in mature form Potential
PRO_0000231644

Regions

Domain133 – 21078UPAR/Ly6 1
Domain325 – 37753UPAR/Ly6 2

Amino acid modifications

Lipidation4081GPI-anchor amidated glycine Potential
Glycosylation1891N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence129 – 14517GSLRC…GCLEG → MMGAAEGPFGKAEQVDS in isoform 3.
VSP_017858
Alternative sequence146 – 437292Missing in isoform 3.
VSP_017859
Alternative sequence210 – 24839TCHRG…CQETL → QLKPVGLLEEHNVGEKERGQ GQHQCVQLDWQKRAATGIF in isoform 2.
VSP_017860
Alternative sequence249 – 437189Missing in isoform 2.
VSP_017861
Natural variant31A → P. Ref.2 Ref.3 Ref.6 Ref.11
Corresponds to variant rs45441892 [ dbSNP | Ensembl ].
VAR_025858
Natural variant1191L → F. Ref.2 Ref.11
VAR_026156
Natural variant2511L → I. Ref.1 Ref.2 Ref.4 Ref.5
Corresponds to variant rs10425835 [ dbSNP | Ensembl ].
VAR_025859
Natural variant3231R → Q. Ref.2 Ref.11
VAR_026157
Natural variant3481A → T. Ref.6 Ref.10
Corresponds to variant rs17856829 [ dbSNP | Ensembl ].
VAR_025860
Natural variant3791F → S. Ref.2 Ref.11
VAR_026158

Experimental info

Sequence conflict891H → Y in CAC83724. Ref.3
Sequence conflict1841G → V in CAC83724. Ref.3
Sequence conflict1941I → F in CAC83724. Ref.3
Sequence conflict204 – 2052NR → DM in CAC83724. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 4, 2006. Version 2.
Checksum: 3D4716F221934910

FASTA43746,363
        10         20         30         40         50         60 
MSAVLLLALL GFILPLPGVQ ALLCQFGTVQ HVWKVSDLPR QWTPKNTSCD SGLGCQDTLM 

        70         80         90        100        110        120 
LIESGPQVSL VLSKGCTEAK DQEPRVTEHR MGPGLSLISY TFVCRQEDFC NNLVNSLPLW 

       130        140        150        160        170        180 
APQPPADPGS LRCPVCLSME GCLEGTTEEI CPKGTTHCYD GLLRLRGGGI FSNLRVQGCM 

       190        200        210        220        230        240 
PQPGCNLLNG TQEIGPVGMT ENCNRKDFLT CHRGTTIMTH GNLAQEPTDW TTSNTEMCEV 

       250        260        270        280        290        300 
GQVCQETLLL LDVGLTSTLV GTKGCSTVGA QNSQKTTIHS APPGVLVASY THFCSSDLCN 

       310        320        330        340        350        360 
SASSSSVLLN SLPPQAAPVP GDRQCPTCVQ PLGTCSSGSP RMTCPRGATH CYDGYIHLSG 

       370        380        390        400        410        420 
GGLSTKMSIQ GCVAQPSSFL LNHTRQIGIF SAREKRDVQP PASQHEGGGA EGLESLTWGV 

       430 
GLALAPALWW GVVCPSC 

« Hide

Isoform 2 [UniParc].

Checksum: F39533690DE33364
Show »

FASTA24827,061
Isoform 3 [UniParc].

Checksum: B213C498B4DFE92A
Show »

FASTA14515,683

References

« Hide 'large scale' references
[1]"Cloning of PRV-1, a novel member of the uPAR receptor superfamily, which is overexpressed in polycythemia rubra vera."
Temerinac S., Klippel S., Strunck E., Roeder S., Luebbert M., Lange W., Azemar M., Meinhardt G., Schaefer H.-E., Pahl H.L.
Blood 95:2569-2576(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ILE-251, TISSUE SPECIFICITY, INDUCTION.
[2]"Molecular basis of the neutrophil glycoprotein NB1 (CD177) involved in the pathogenesis of immunenneutropenia and transfusion reactions."
Kissel K., Santoso S., Hofmann C., Stroncek D., Bux J.
Eur. J. Immunol. 31:1301-1309(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 35-45; 167-175 AND 386-393, VARIANTS PRO-3; PHE-119; ILE-251; GLN-323 AND SER-379, MASS SPECTROMETRY, GLYCOSYLATION.
[3]"Molecular basis of NB1 (HNA-2a, CD177) deficiency."
Kissel K., Scheffler S., Kerowgan M., Bux J.
Blood 99:4231-4233(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), VARIANT PRO-3.
[4]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ILE-251.
[5]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ILE-251.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS PRO-3 AND THR-348.
Tissue: Colon.
[7]"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z., Henzel W.J.
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 22-36.
[8]"Biochemical characterization of PRV-1, a novel hematopoietic cell surface receptor, which is overexpressed in polycythemia rubra vera."
Klippel S., Strunck E., Busse C.E., Behringer D., Pahl H.L.
Blood 100:2441-2448(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, SHEDDING.
[9]"Overexpression of the polycythemia rubra vera-1 gene in essential thrombocythemia."
Teofili L., Martini M., Luongo M., Di Mario A., Leone G., De Stefano V., Larocca L.M.
J. Clin. Oncol. 20:4249-4254(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, TISSUE SPECIFICITY.
[10]"Lack of NB1 GP (CD177/HNA-2a) gene transcription in NB1 GP-neutrophils from NB1 GP-expressing individuals and association of low expression with NB1 gene polymorphisms."
Wolff J., Brendel C., Fink L., Bohle R.M., Kissel K., Bux J.
Blood 102:731-733(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT THR-348, POLYMORPHISM.
[11]"CD177 polymorphisms: correlation between high-frequency single nucleotide polymorphisms and neutrophil surface protein expression."
Caruccio L., Walkovich K., Bettinotti M., Schuller R., Stroncek D.
Transfusion 44:77-82(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS PRO-3; PHE-119; GLN-323 AND SER-379.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF146747 mRNA. Translation: AAG00895.1.
AJ290452 mRNA. Translation: CAC44459.1.
AJ305326 mRNA. Translation: CAC83758.1.
AJ310433 mRNA. Translation: CAC83724.1.
AY358932 mRNA. Translation: AAQ89291.1.
BT020111 mRNA. Translation: AAV38914.1.
BC029167 mRNA. Translation: AAH29167.1.
CCDSCCDS62700.1. [Q8N6Q3-1]
RefSeqNP_065139.2. NM_020406.3.
UniGeneHs.232165.

3D structure databases

ProteinModelPortalQ8N6Q3.
SMRQ8N6Q3. Positions 175-203.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121389. 3 interactions.
IntActQ8N6Q3. 2 interactions.
MINTMINT-1453771.
STRING9606.ENSP00000388794.

Polymorphism databases

DMDM91208246.

Proteomic databases

PaxDbQ8N6Q3.
PRIDEQ8N6Q3.

Protocols and materials databases

DNASU57126.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000378012; ENSP00000367251; ENSG00000204936. [Q8N6Q3-3]
ENST00000457794; ENSP00000388794; ENSG00000204936. [Q8N6Q3-3]
GeneID57126.
KEGGhsa:57126.
UCSCuc002owi.3. human. [Q8N6Q3-1]
uc021uvf.1. human. [Q8N6Q3-3]

Organism-specific databases

CTD57126.
GeneCardsGC19P043857.
H-InvDBHIX0017865.
HGNCHGNC:30072. CD177.
MIM162860. gene.
neXtProtNX_Q8N6Q3.
PharmGKBPA142672147.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG75007.
HOVERGENHBG081064.
KOK06552.
OMASSACICA.
PhylomeDBQ8N6Q3.

Enzyme and pathway databases

ReactomeREACT_604. Hemostasis.

Gene expression databases

ArrayExpressQ8N6Q3.
BgeeQ8N6Q3.
CleanExHS_CD177.
GenevestigatorQ8N6Q3.

Family and domain databases

InterProIPR001526. LY6_UPAR.
[Graphical view]
PfamPF00021. UPAR_LY6. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCD177.
GenomeRNAi57126.
NextBio63021.
PROQ8N6Q3.
SOURCESearch...

Entry information

Entry nameCD177_HUMAN
AccessionPrimary (citable) accession number: Q8N6Q3
Secondary accession number(s): Q711Q2 expand/collapse secondary AC list , Q8NCV9, Q96QH1, Q9HDA5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: April 4, 2006
Last modified: July 9, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries