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Protein

CD177 antigen

Gene

CD177

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407). In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980). Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308). However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:23461681). Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed:17244676, PubMed:18462208). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed:23202369).7 Publications

GO - Molecular functioni

  • calcium-dependent protein binding Source: UniProtKB
  • integrin binding Source: UniProtKB
  • protease binding Source: UniProtKB

GO - Biological processi

  • blood coagulation Source: Reactome
  • cell adhesion Source: UniProtKB
  • cell-cell adhesion via plasma-membrane adhesion molecules Source: UniProtKB
  • cell-cell junction maintenance Source: UniProtKB
  • leukocyte cell-cell adhesion Source: UniProtKB
  • leukocyte migration Source: Reactome
  • neutrophil degranulation Source: Reactome
  • neutrophil extravasation Source: UniProtKB
  • neutrophil migration Source: UniProtKB
  • positive regulation of neutrophil degranulation Source: UniProtKB
  • positive regulation of superoxide anion generation Source: UniProtKB
  • protein localization to cell surface Source: UniProtKB
  • regulation of endocytosis Source: UniProtKB
  • regulation of integrin-mediated signaling pathway Source: UniProtKB

Keywordsi

Biological processCell adhesion, Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiR-HSA-140875. Common Pathway of Fibrin Clot Formation.
R-HSA-202733. Cell surface interactions at the vascular wall.
R-HSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
CD177 antigenImported
Alternative name(s):
Human neutrophil alloantigen 2a1 Publication
Short name:
HNA-2a1 Publication
NB1 glycoprotein1 Publication
Short name:
NB1 GP1 Publication
Polycythemia rubra vera protein 11 Publication
Short name:
PRV-11 Publication
CD_antigen: CD177
Gene namesi
Name:CD177Imported
Synonyms:NB1, PRV11 Publication
ORF Names:UNQ595/PRO1181
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000204936.9.
HGNCiHGNC:30072. CD177.
MIMi162860. gene.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi57126.
OpenTargetsiENSG00000204936.
PharmGKBiPA142672147.

Polymorphism and mutation databases

BioMutaiCD177.
DMDMi91208246.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000023164322 – 408CD177 antigenAdd BLAST387
PropeptideiPRO_0000231644409 – 437Removed in mature formSequence analysisAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi189N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi408GPI-anchor amidated glycineSequence analysis1

Post-translational modificationi

N-glycosylated.2 Publications
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).2 Publications

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ8N6Q3.
PeptideAtlasiQ8N6Q3.
PRIDEiQ8N6Q3.

Expressioni

Tissue specificityi

Highly expressed in normal bone marrow and weakly expressed in fetal liver (PubMed:10753836). During neutrophil differentiation, expression begins at the metamyelocyte stage and continues throughout the subsequent stages (at protein level) (PubMed:17244676, PubMed:18462208, PubMed:24926686). Expressed by a subset of mature neutrophils (at protein level) (PubMed:10753836, PubMed:28240246, PubMed:12377969, PubMed:18462208, PubMed:12675722, PubMed:17244676, PubMed:17580308, PubMed:21193407, PubMed:24926686, PubMed:28807980, PubMed:27227454). The percentage of neutrophils expressing CD177 varies across the population (PubMed:17244676, PubMed:27227454). Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET) (PubMed:10753836, PubMed:12377969).10 Publications

Developmental stagei

Expressed in neonatal and adult neutrophils.1 Publication

Inductioni

By CSF3/G-CSF in resting granulocytes (PubMed:10753836). Induced during CSF3/G-CSF-mediated neutrophil differentiation (PubMed:17244676, PubMed:18462208). Induced during pregnancy (PubMed:12675722). Induced in patients with polycythemia vera (PV) and with essential thrombocythemia (ET) (PubMed:10753836, PubMed:12377969).5 Publications

Gene expression databases

BgeeiENSG00000204936.
CleanExiHS_CD177.
ExpressionAtlasiQ8N6Q3. baseline and differential.
GenevisibleiQ8N6Q3. HS.

Organism-specific databases

HPAiHPA041820.
HPA046601.

Interactioni

Subunit structurei

Found in a complex with integrin ITGAM/CD11b and ITGB2/CD18 (PubMed:28807980, PubMed:21193407). Interacts with PECAM1 (via Ig-like C2-type domain 6); the interaction is Ca2+-dependent; the interaction is direct (PubMed:17580308). Interacts with serine protease PRTN3/myeloblastin; the interaction tethers PRTN3 to the cell surface; the interaction is direct (PubMed:17244676, PubMed:28240246).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KCNIP3Q9Y2W76EBI-747170,EBI-751501

GO - Molecular functioni

  • calcium-dependent protein binding Source: UniProtKB
  • integrin binding Source: UniProtKB
  • protease binding Source: UniProtKB

Protein-protein interaction databases

BioGridi121389. 3 interactors.
IntActiQ8N6Q3. 2 interactors.
MINTiMINT-1453771.
STRINGi9606.ENSP00000367248.

Structurei

3D structure databases

ProteinModelPortaliQ8N6Q3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini133 – 210UPAR/Ly6 1Add BLAST78
Domaini325 – 377UPAR/Ly6 2Add BLAST53

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410J207. Eukaryota.
ENOG4111D7X. LUCA.
GeneTreeiENSGT00530000063351.
HOVERGENiHBG081064.
InParanoidiQ8N6Q3.
KOiK06552.
PhylomeDBiQ8N6Q3.

Family and domain databases

InterProiView protein in InterPro
IPR016054. LY6_UPA_recep-like.
PfamiView protein in Pfam
PF00021. UPAR_LY6. 2 hits.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N6Q3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAVLLLALL GFILPLPGVQ ALLCQFGTVQ HVWKVSDLPR QWTPKNTSCD
60 70 80 90 100
SGLGCQDTLM LIESGPQVSL VLSKGCTEAK DQEPRVTEHR MGPGLSLISY
110 120 130 140 150
TFVCRQEDFC NNLVNSLPLW APQPPADPGS LRCPVCLSME GCLEGTTEEI
160 170 180 190 200
CPKGTTHCYD GLLRLRGGGI FSNLRVQGCM PQPGCNLLNG TQEIGPVGMT
210 220 230 240 250
ENCNRKDFLT CHRGTTIMTH GNLAQEPTDW TTSNTEMCEV GQVCQETLLL
260 270 280 290 300
LDVGLTSTLV GTKGCSTVGA QNSQKTTIHS APPGVLVASY THFCSSDLCN
310 320 330 340 350
SASSSSVLLN SLPPQAAPVP GDRQCPTCVQ PLGTCSSGSP RMTCPRGATH
360 370 380 390 400
CYDGYIHLSG GGLSTKMSIQ GCVAQPSSFL LNHTRQIGIF SAREKRDVQP
410 420 430
PASQHEGGGA EGLESLTWGV GLALAPALWW GVVCPSC
Length:437
Mass (Da):46,363
Last modified:April 4, 2006 - v2
Checksum:i3D4716F221934910
GO
Isoform 2 (identifier: Q8N6Q3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     210-248: TCHRGTTIMT...EVGQVCQETL → QLKPVGLLEE...WQKRAATGIF
     249-437: Missing.

Note: Associated with CD177-negative phenotype.
Show »
Length:248
Mass (Da):27,061
Checksum:iF39533690DE33364
GO
Isoform 3 (identifier: Q8N6Q3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     129-145: GSLRCPVCLSMEGCLEG → MMGAAEGPFGKAEQVDS
     146-437: Missing.

Note: Associated with CD177-negative phenotype.
Show »
Length:145
Mass (Da):15,683
Checksum:iB213C498B4DFE92A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89H → Y in CAC83724 (PubMed:12010833).Curated1
Sequence conflicti184G → V in CAC83724 (PubMed:12010833).Curated1
Sequence conflicti194I → F in CAC83724 (PubMed:12010833).Curated1
Sequence conflicti204 – 205NR → DM in CAC83724 (PubMed:12010833).Curated2

Mass spectrometryi

Molecular mass is 50556 Da from positions 22 - 408. Determined by MALDI. 1 Publication

Polymorphismi

There is a significant association between the variants Ala-3, Leu-251 and Thr-348 and a low expression of CD177 on neutrophils (PubMed:12623849, PubMed:14692971). Expression of CD177 on neutrophils is a trait determined by ratio of CD177/CD177P1 alleles (PubMed:27227454). The phenotype of CD177 null neutrophils is due to recombination between exon 7 of CD177 and the pseudogene CD177P1 through gene conversion, changing Lys-263 codon into stop codon (PubMed:27227454). The lack of CD177 expression affects 1-10 percent of the population placing them at risk for formation of anti-neutrophil antibodies that can cause transfusion-related acute lung injury and neonatal alloimmune neutropenia.3 Publications

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0258583A → P5 PublicationsCorresponds to variant dbSNP:rs45441892Ensembl.1
Natural variantiVAR_07975431H → L1 PublicationCorresponds to variant dbSNP:rs45553433Ensembl.1
Natural variantiVAR_026156119L → F2 Publications1
Natural variantiVAR_025859251L → I5 PublicationsCorresponds to variant dbSNP:rs10425835Ensembl.1
Natural variantiVAR_079755261G → A1 Publication1
Natural variantiVAR_026157323R → Q2 Publications1
Natural variantiVAR_025860348A → T3 PublicationsCorresponds to variant dbSNP:rs17856829Ensembl.1
Natural variantiVAR_026158379F → S2 Publications1
Natural variantiVAR_079756431G → R1 PublicationCorresponds to variant dbSNP:rs78718189Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017858129 – 145GSLRC…GCLEG → MMGAAEGPFGKAEQVDS in isoform 3. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_017859146 – 437Missing in isoform 3. 1 PublicationAdd BLAST292
Alternative sequenceiVSP_017860210 – 248TCHRG…CQETL → QLKPVGLLEEHNVGEKERGQ GQHQCVQLDWQKRAATGIF in isoform 2. 1 PublicationAdd BLAST39
Alternative sequenceiVSP_017861249 – 437Missing in isoform 2. 1 PublicationAdd BLAST189

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146747 mRNA. Translation: AAG00895.1.
AJ290452 mRNA. Translation: CAC44459.1.
AJ305326 mRNA. Translation: CAC83758.1.
AJ310433 mRNA. Translation: CAC83724.1.
AY358932 mRNA. Translation: AAQ89291.1.
BT020111 mRNA. Translation: AAV38914.1.
BC029167 mRNA. Translation: AAH29167.1.
CCDSiCCDS62700.1. [Q8N6Q3-1]
RefSeqiNP_065139.2. NM_020406.3.
UniGeneiHs.232165.

Genome annotation databases

EnsembliENST00000378012; ENSP00000367251; ENSG00000204936. [Q8N6Q3-3]
GeneIDi57126.
KEGGihsa:57126.
UCSCiuc060zla.1. human. [Q8N6Q3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCD177_HUMAN
AccessioniPrimary (citable) accession number: Q8N6Q3
Secondary accession number(s): Q711Q2
, Q8NCV9, Q96QH1, Q9HDA5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: April 4, 2006
Last modified: December 20, 2017
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot