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Q8N6Q3

- CD177_HUMAN

UniProt

Q8N6Q3 - CD177_HUMAN

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Protein

CD177 antigen

Gene

CD177

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GO - Biological processi

  1. blood coagulation Source: Reactome
  2. leukocyte migration Source: Reactome
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_12051. Cell surface interactions at the vascular wall.

Names & Taxonomyi

Protein namesi
Recommended name:
CD177 antigen
Alternative name(s):
Human neutrophil alloantigen 2a
Short name:
HNA-2a
NB1 glycoprotein
Short name:
NB1 GP
Polycythemia rubra vera protein 1
Short name:
PRV-1
CD_antigen: CD177
Gene namesi
Name:CD177
Synonyms:NB1, PRV1
ORF Names:UNQ595/PRO1181
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:30072. CD177.

Subcellular locationi

Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: UniProtKB
  3. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142672147.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 408387CD177 antigenPRO_0000231643Add
BLAST
Propeptidei409 – 43729Removed in mature formSequence AnalysisPRO_0000231644Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi189 – 1891N-linked (GlcNAc...)Sequence Analysis
Lipidationi408 – 4081GPI-anchor amidated glycineSequence Analysis

Post-translational modificationi

N-glycosylated.2 Publications
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ8N6Q3.
PRIDEiQ8N6Q3.

Expressioni

Tissue specificityi

Highly expressed in normal bone marrow and weakly expressed in fetal liver. Expressed on neutrophils. Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET).2 Publications

Inductioni

By CSF3 in resting granulocytes. Induced in patients with polycythemia vera (PV) and with essential thrombocythemia (ET).2 Publications

Gene expression databases

BgeeiQ8N6Q3.
CleanExiHS_CD177.
GenevestigatoriQ8N6Q3.

Interactioni

Protein-protein interaction databases

BioGridi121389. 3 interactions.
IntActiQ8N6Q3. 2 interactions.
MINTiMINT-1453771.
STRINGi9606.ENSP00000388794.

Structurei

3D structure databases

ProteinModelPortaliQ8N6Q3.
SMRiQ8N6Q3. Positions 175-203.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini133 – 21078UPAR/Ly6 1Add
BLAST
Domaini325 – 37753UPAR/Ly6 2Add
BLAST

Sequence similaritiesi

Contains 2 UPAR/Ly6 domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG75007.
GeneTreeiENSGT00530000063351.
HOVERGENiHBG081064.
InParanoidiQ8N6Q3.
KOiK06552.
OMAiSSACICA.
PhylomeDBiQ8N6Q3.

Family and domain databases

InterProiIPR001526. LY6_UPAR.
[Graphical view]
PfamiPF00021. UPAR_LY6. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8N6Q3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAVLLLALL GFILPLPGVQ ALLCQFGTVQ HVWKVSDLPR QWTPKNTSCD
60 70 80 90 100
SGLGCQDTLM LIESGPQVSL VLSKGCTEAK DQEPRVTEHR MGPGLSLISY
110 120 130 140 150
TFVCRQEDFC NNLVNSLPLW APQPPADPGS LRCPVCLSME GCLEGTTEEI
160 170 180 190 200
CPKGTTHCYD GLLRLRGGGI FSNLRVQGCM PQPGCNLLNG TQEIGPVGMT
210 220 230 240 250
ENCNRKDFLT CHRGTTIMTH GNLAQEPTDW TTSNTEMCEV GQVCQETLLL
260 270 280 290 300
LDVGLTSTLV GTKGCSTVGA QNSQKTTIHS APPGVLVASY THFCSSDLCN
310 320 330 340 350
SASSSSVLLN SLPPQAAPVP GDRQCPTCVQ PLGTCSSGSP RMTCPRGATH
360 370 380 390 400
CYDGYIHLSG GGLSTKMSIQ GCVAQPSSFL LNHTRQIGIF SAREKRDVQP
410 420 430
PASQHEGGGA EGLESLTWGV GLALAPALWW GVVCPSC
Length:437
Mass (Da):46,363
Last modified:April 4, 2006 - v2
Checksum:i3D4716F221934910
GO
Isoform 2 (identifier: Q8N6Q3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     210-248: TCHRGTTIMT...EVGQVCQETL → QLKPVGLLEE...WQKRAATGIF
     249-437: Missing.

Note: Associated with CD177-negative phenotype.

Show »
Length:248
Mass (Da):27,061
Checksum:iF39533690DE33364
GO
Isoform 3 (identifier: Q8N6Q3-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     129-145: GSLRCPVCLSMEGCLEG → MMGAAEGPFGKAEQVDS
     146-437: Missing.

Note: Associated with CD177-negative phenotype.

Show »
Length:145
Mass (Da):15,683
Checksum:iB213C498B4DFE92A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891H → Y in CAC83724. (PubMed:12010833)Curated
Sequence conflicti184 – 1841G → V in CAC83724. (PubMed:12010833)Curated
Sequence conflicti194 – 1941I → F in CAC83724. (PubMed:12010833)Curated
Sequence conflicti204 – 2052NR → DM in CAC83724. (PubMed:12010833)Curated

Mass spectrometryi

Molecular mass is 50556 Da from positions 22 - 408. Determined by MALDI. 1 Publication

Polymorphismi

There is a significant association between the variants Ala-3, Leu-251 and Thr-348 and a low expression of CD177 on neutrophils.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti3 – 31A → P.4 Publications
Corresponds to variant rs45441892 [ dbSNP | Ensembl ].
VAR_025858
Natural varianti119 – 1191L → F.2 Publications
VAR_026156
Natural varianti251 – 2511L → I.4 Publications
Corresponds to variant rs10425835 [ dbSNP | Ensembl ].
VAR_025859
Natural varianti323 – 3231R → Q.2 Publications
VAR_026157
Natural varianti348 – 3481A → T.2 Publications
Corresponds to variant rs17856829 [ dbSNP | Ensembl ].
VAR_025860
Natural varianti379 – 3791F → S.2 Publications
VAR_026158

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei129 – 14517GSLRC…GCLEG → MMGAAEGPFGKAEQVDS in isoform 3. 1 PublicationVSP_017858Add
BLAST
Alternative sequencei146 – 437292Missing in isoform 3. 1 PublicationVSP_017859Add
BLAST
Alternative sequencei210 – 24839TCHRG…CQETL → QLKPVGLLEEHNVGEKERGQ GQHQCVQLDWQKRAATGIF in isoform 2. 1 PublicationVSP_017860Add
BLAST
Alternative sequencei249 – 437189Missing in isoform 2. 1 PublicationVSP_017861Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF146747 mRNA. Translation: AAG00895.1.
AJ290452 mRNA. Translation: CAC44459.1.
AJ305326 mRNA. Translation: CAC83758.1.
AJ310433 mRNA. Translation: CAC83724.1.
AY358932 mRNA. Translation: AAQ89291.1.
BT020111 mRNA. Translation: AAV38914.1.
BC029167 mRNA. Translation: AAH29167.1.
CCDSiCCDS62700.1. [Q8N6Q3-1]
RefSeqiNP_065139.2. NM_020406.3.
UniGeneiHs.232165.

Genome annotation databases

EnsembliENST00000378012; ENSP00000367251; ENSG00000204936. [Q8N6Q3-3]
GeneIDi57126.
KEGGihsa:57126.
UCSCiuc002owi.3. human. [Q8N6Q3-1]
uc021uvf.1. human. [Q8N6Q3-3]

Polymorphism databases

DMDMi91208246.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF146747 mRNA. Translation: AAG00895.1 .
AJ290452 mRNA. Translation: CAC44459.1 .
AJ305326 mRNA. Translation: CAC83758.1 .
AJ310433 mRNA. Translation: CAC83724.1 .
AY358932 mRNA. Translation: AAQ89291.1 .
BT020111 mRNA. Translation: AAV38914.1 .
BC029167 mRNA. Translation: AAH29167.1 .
CCDSi CCDS62700.1. [Q8N6Q3-1 ]
RefSeqi NP_065139.2. NM_020406.3.
UniGenei Hs.232165.

3D structure databases

ProteinModelPortali Q8N6Q3.
SMRi Q8N6Q3. Positions 175-203.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121389. 3 interactions.
IntActi Q8N6Q3. 2 interactions.
MINTi MINT-1453771.
STRINGi 9606.ENSP00000388794.

Polymorphism databases

DMDMi 91208246.

Proteomic databases

PaxDbi Q8N6Q3.
PRIDEi Q8N6Q3.

Protocols and materials databases

DNASUi 57126.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000378012 ; ENSP00000367251 ; ENSG00000204936 . [Q8N6Q3-3 ]
GeneIDi 57126.
KEGGi hsa:57126.
UCSCi uc002owi.3. human. [Q8N6Q3-1 ]
uc021uvf.1. human. [Q8N6Q3-3 ]

Organism-specific databases

CTDi 57126.
GeneCardsi GC19P043857.
H-InvDB HIX0017865.
HGNCi HGNC:30072. CD177.
MIMi 162860. gene.
neXtProti NX_Q8N6Q3.
PharmGKBi PA142672147.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG75007.
GeneTreei ENSGT00530000063351.
HOVERGENi HBG081064.
InParanoidi Q8N6Q3.
KOi K06552.
OMAi SSACICA.
PhylomeDBi Q8N6Q3.

Enzyme and pathway databases

Reactomei REACT_12051. Cell surface interactions at the vascular wall.

Miscellaneous databases

GeneWikii CD177.
GenomeRNAii 57126.
NextBioi 63021.
PROi Q8N6Q3.
SOURCEi Search...

Gene expression databases

Bgeei Q8N6Q3.
CleanExi HS_CD177.
Genevestigatori Q8N6Q3.

Family and domain databases

InterProi IPR001526. LY6_UPAR.
[Graphical view ]
Pfami PF00021. UPAR_LY6. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of PRV-1, a novel member of the uPAR receptor superfamily, which is overexpressed in polycythemia rubra vera."
    Temerinac S., Klippel S., Strunck E., Roeder S., Luebbert M., Lange W., Azemar M., Meinhardt G., Schaefer H.-E., Pahl H.L.
    Blood 95:2569-2576(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ILE-251, TISSUE SPECIFICITY, INDUCTION.
  2. "Molecular basis of the neutrophil glycoprotein NB1 (CD177) involved in the pathogenesis of immunenneutropenia and transfusion reactions."
    Kissel K., Santoso S., Hofmann C., Stroncek D., Bux J.
    Eur. J. Immunol. 31:1301-1309(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 35-45; 167-175 AND 386-393, VARIANTS PRO-3; PHE-119; ILE-251; GLN-323 AND SER-379, MASS SPECTROMETRY, GLYCOSYLATION.
  3. "Molecular basis of NB1 (HNA-2a, CD177) deficiency."
    Kissel K., Scheffler S., Kerowgan M., Bux J.
    Blood 99:4231-4233(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), VARIANT PRO-3.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ILE-251.
  5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ILE-251.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS PRO-3 AND THR-348.
    Tissue: Colon.
  7. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
    Zhang Z., Henzel W.J.
    Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-36.
  8. "Biochemical characterization of PRV-1, a novel hematopoietic cell surface receptor, which is overexpressed in polycythemia rubra vera."
    Klippel S., Strunck E., Busse C.E., Behringer D., Pahl H.L.
    Blood 100:2441-2448(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, GLYCOSYLATION, SHEDDING.
  9. "Overexpression of the polycythemia rubra vera-1 gene in essential thrombocythemia."
    Teofili L., Martini M., Luongo M., Di Mario A., Leone G., De Stefano V., Larocca L.M.
    J. Clin. Oncol. 20:4249-4254(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, TISSUE SPECIFICITY.
  10. "Lack of NB1 GP (CD177/HNA-2a) gene transcription in NB1 GP-neutrophils from NB1 GP-expressing individuals and association of low expression with NB1 gene polymorphisms."
    Wolff J., Brendel C., Fink L., Bohle R.M., Kissel K., Bux J.
    Blood 102:731-733(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT THR-348, POLYMORPHISM.
  11. "CD177 polymorphisms: correlation between high-frequency single nucleotide polymorphisms and neutrophil surface protein expression."
    Caruccio L., Walkovich K., Bettinotti M., Schuller R., Stroncek D.
    Transfusion 44:77-82(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS PRO-3; PHE-119; GLN-323 AND SER-379.

Entry informationi

Entry nameiCD177_HUMAN
AccessioniPrimary (citable) accession number: Q8N6Q3
Secondary accession number(s): Q711Q2
, Q8NCV9, Q96QH1, Q9HDA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: April 4, 2006
Last modified: October 29, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3