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Q8N6M0

- OTU6B_HUMAN

UniProt

Q8N6M0 - OTU6B_HUMAN

Protein

OTU domain-containing protein 6B

Gene

OTUD6B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei155 – 1551By similarity
    Active sitei158 – 1581NucleophileBy similarity
    Active sitei277 – 2771By similarity

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    OTU domain-containing protein 6B (EC:3.4.19.12)
    Alternative name(s):
    DUBA-5
    Gene namesi
    Name:OTUD6B
    Synonyms:DUBA5
    ORF Names:CGI-77
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:24281. OTUD6B.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142671219.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 293293OTU domain-containing protein 6BPRO_0000076279Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine2 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8N6M0.
    PaxDbiQ8N6M0.
    PRIDEiQ8N6M0.

    PTM databases

    PhosphoSiteiQ8N6M0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8N6M0.
    BgeeiQ8N6M0.
    CleanExiHS_OTUD6B.
    GenevestigatoriQ8N6M0.

    Organism-specific databases

    HPAiHPA024046.
    HPA024503.
    HPA024772.

    Interactioni

    Protein-protein interaction databases

    BioGridi119647. 16 interactions.
    IntActiQ8N6M0. 5 interactions.
    STRINGi9606.ENSP00000285420.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8N6M0.
    SMRiQ8N6M0. Positions 138-284.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini147 – 284138OTUPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni152 – 1587Cys-loopBy similarity
    Regioni219 – 22911Variable-loopBy similarityAdd
    BLAST
    Regioni267 – 27711His-loopBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 OTU domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5539.
    HOGENOMiHOG000192906.
    HOVERGENiHBG080227.
    InParanoidiQ8N6M0.
    KOiK18342.
    OrthoDBiEOG7WT42W.
    PhylomeDBiQ8N6M0.
    TreeFamiTF315010.

    Family and domain databases

    InterProiIPR003323. OTU.
    [Graphical view]
    PfamiPF02338. OTU. 1 hit.
    [Graphical view]
    PROSITEiPS50802. OTU. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8N6M0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEAVLTEELD EEEQLLRRHR KEKKELQAKI QGMKNAVPKN DKKRRKQLTE    50
    DVAKLEKEME QKHREELEQL KLTTKENKID SVAVNISNLV LENQPPRISK 100
    AQKRREKKAA LEKEREERIA EAEIENLTGA RHMESEKLAQ ILAARQLEIK 150
    QIPSDGHCMY KAIEDQLKEK DCALTVVALR SQTAEYMQSH VEDFLPFLTN 200
    PNTGDMYTPE EFQKYCEDIV NTAAWGGQLE LRALSHILQT PIEIIQADSP 250
    PIIVGEEYSK KPLILVYMRH AYGLGEHYNS VTRLVNIVTE NCS 293
    Length:293
    Mass (Da):33,813
    Last modified:October 1, 2002 - v1
    Checksum:i468DB88E2637D869
    GO
    Isoform 2 (identifier: Q8N6M0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-105: MEAVLTEELD...PRISKAQKRR → MISK

    Note: No experimental confirmation available.

    Show »
    Length:192
    Mass (Da):21,809
    Checksum:iDB3F389CEE3BEB3E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti285 – 2939VNIVTENCS → GKHSY in AAD34073. (PubMed:10810093)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti283 – 2831R → Q.1 Publication
    Corresponds to variant rs3210518 [ dbSNP | Ensembl ].
    VAR_034144

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 105105MEAVL…AQKRR → MISK in isoform 2. 1 PublicationVSP_055378Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF151836 mRNA. Translation: AAD34073.1.
    AK291646 mRNA. Translation: BAF84335.1.
    AK293843 mRNA. Translation: BAG57241.1.
    AC087439 Genomic DNA. No translation available.
    BC029760 mRNA. Translation: AAH29760.1.
    AL137441 mRNA. Translation: CAB70738.1.
    CCDSiCCDS69513.1. [Q8N6M0-2]
    PIRiT46264.
    RefSeqiNP_001273674.1. NM_001286745.1.
    NP_057107.3. NM_016023.3.
    UniGeneiHs.30532.

    Genome annotation databases

    EnsembliENST00000404789; ENSP00000384190; ENSG00000155100. [Q8N6M0-2]
    GeneIDi51633.
    KEGGihsa:51633.
    UCSCiuc003yeu.4. human. [Q8N6M0-1]

    Polymorphism databases

    DMDMi74729149.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF151836 mRNA. Translation: AAD34073.1 .
    AK291646 mRNA. Translation: BAF84335.1 .
    AK293843 mRNA. Translation: BAG57241.1 .
    AC087439 Genomic DNA. No translation available.
    BC029760 mRNA. Translation: AAH29760.1 .
    AL137441 mRNA. Translation: CAB70738.1 .
    CCDSi CCDS69513.1. [Q8N6M0-2 ]
    PIRi T46264.
    RefSeqi NP_001273674.1. NM_001286745.1.
    NP_057107.3. NM_016023.3.
    UniGenei Hs.30532.

    3D structure databases

    ProteinModelPortali Q8N6M0.
    SMRi Q8N6M0. Positions 138-284.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119647. 16 interactions.
    IntActi Q8N6M0. 5 interactions.
    STRINGi 9606.ENSP00000285420.

    PTM databases

    PhosphoSitei Q8N6M0.

    Polymorphism databases

    DMDMi 74729149.

    Proteomic databases

    MaxQBi Q8N6M0.
    PaxDbi Q8N6M0.
    PRIDEi Q8N6M0.

    Protocols and materials databases

    DNASUi 51633.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000404789 ; ENSP00000384190 ; ENSG00000155100 . [Q8N6M0-2 ]
    GeneIDi 51633.
    KEGGi hsa:51633.
    UCSCi uc003yeu.4. human. [Q8N6M0-1 ]

    Organism-specific databases

    CTDi 51633.
    GeneCardsi GC08P092082.
    H-InvDB HIX0201303.
    HGNCi HGNC:24281. OTUD6B.
    HPAi HPA024046.
    HPA024503.
    HPA024772.
    MIMi 612021. gene.
    neXtProti NX_Q8N6M0.
    PharmGKBi PA142671219.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5539.
    HOGENOMi HOG000192906.
    HOVERGENi HBG080227.
    InParanoidi Q8N6M0.
    KOi K18342.
    OrthoDBi EOG7WT42W.
    PhylomeDBi Q8N6M0.
    TreeFami TF315010.

    Miscellaneous databases

    GeneWikii OTUD6B.
    GenomeRNAii 51633.
    NextBioi 35471351.
    PROi Q8N6M0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8N6M0.
    Bgeei Q8N6M0.
    CleanExi HS_OTUD6B.
    Genevestigatori Q8N6M0.

    Family and domain databases

    InterProi IPR003323. OTU.
    [Graphical view ]
    Pfami PF02338. OTU. 1 hit.
    [Graphical view ]
    PROSITEi PS50802. OTU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
      Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
      Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Cerebellum and Placenta.
    3. "DNA sequence and analysis of human chromosome 8."
      Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
      , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
      Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lymph.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 67-293 (ISOFORM 1), VARIANT GLN-283.
      Tissue: Amygdala.
    6. "OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis."
      Mevissen T.E., Hospenthal M.K., Geurink P.P., Elliott P.R., Akutsu M., Arnaudo N., Ekkebus R., Kulathu Y., Wauer T., El Oualid F., Freund S.M., Ovaa H., Komander D.
      Cell 154:169-184(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiOTU6B_HUMAN
    AccessioniPrimary (citable) accession number: Q8N6M0
    Secondary accession number(s): A8K6I1
    , B4DEY0, Q9NTA4, Q9Y387
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2006
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    No deubiquitinase activity has been detected when tested (PubMed:23827681). May require some post-translational modification to be active.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3