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Protein

OTU domain-containing protein 6B

Gene

OTUD6B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei155 – 1551By similarity
Active sitei158 – 1581NucleophileBy similarity
Active sitei277 – 2771By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
OTU domain-containing protein 6B (EC:3.4.19.12)
Alternative name(s):
DUBA-5
Gene namesi
Name:OTUD6B
Synonyms:DUBA5
ORF Names:CGI-77
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:24281. OTUD6B.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671219.

Polymorphism and mutation databases

BioMutaiOTUD6B.
DMDMi74729149.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 293293OTU domain-containing protein 6BPRO_0000076279Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8N6M0.
PaxDbiQ8N6M0.
PRIDEiQ8N6M0.

PTM databases

PhosphoSiteiQ8N6M0.

Expressioni

Gene expression databases

BgeeiQ8N6M0.
CleanExiHS_OTUD6B.
ExpressionAtlasiQ8N6M0. baseline and differential.
GenevisibleiQ8N6M0. HS.

Organism-specific databases

HPAiHPA024046.
HPA024503.
HPA024772.

Interactioni

Protein-protein interaction databases

BioGridi119647. 17 interactions.
IntActiQ8N6M0. 5 interactions.
STRINGi9606.ENSP00000285420.

Structurei

3D structure databases

ProteinModelPortaliQ8N6M0.
SMRiQ8N6M0. Positions 138-284.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini147 – 284138OTUPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni152 – 1587Cys-loopBy similarity
Regioni219 – 22911Variable-loopBy similarityAdd
BLAST
Regioni267 – 27711His-loopBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 OTU domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5539.
GeneTreeiENSGT00390000012840.
HOGENOMiHOG000192906.
HOVERGENiHBG080227.
InParanoidiQ8N6M0.
KOiK18342.
OrthoDBiEOG7WT42W.
PhylomeDBiQ8N6M0.
TreeFamiTF315010.

Family and domain databases

InterProiIPR003323. OTU_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N6M0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAVLTEELD EEEQLLRRHR KEKKELQAKI QGMKNAVPKN DKKRRKQLTE
60 70 80 90 100
DVAKLEKEME QKHREELEQL KLTTKENKID SVAVNISNLV LENQPPRISK
110 120 130 140 150
AQKRREKKAA LEKEREERIA EAEIENLTGA RHMESEKLAQ ILAARQLEIK
160 170 180 190 200
QIPSDGHCMY KAIEDQLKEK DCALTVVALR SQTAEYMQSH VEDFLPFLTN
210 220 230 240 250
PNTGDMYTPE EFQKYCEDIV NTAAWGGQLE LRALSHILQT PIEIIQADSP
260 270 280 290
PIIVGEEYSK KPLILVYMRH AYGLGEHYNS VTRLVNIVTE NCS
Length:293
Mass (Da):33,813
Last modified:October 1, 2002 - v1
Checksum:i468DB88E2637D869
GO
Isoform 2 (identifier: Q8N6M0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: MEAVLTEELD...PRISKAQKRR → MISK

Note: No experimental confirmation available.
Show »
Length:192
Mass (Da):21,809
Checksum:iDB3F389CEE3BEB3E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti285 – 2939VNIVTENCS → GKHSY in AAD34073 (PubMed:10810093).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti283 – 2831R → Q.1 Publication
Corresponds to variant rs3210518 [ dbSNP | Ensembl ].
VAR_034144

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 105105MEAVL…AQKRR → MISK in isoform 2. 1 PublicationVSP_055378Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151836 mRNA. Translation: AAD34073.1.
AK291646 mRNA. Translation: BAF84335.1.
AK293843 mRNA. Translation: BAG57241.1.
AC087439 Genomic DNA. No translation available.
BC029760 mRNA. Translation: AAH29760.1.
AL137441 mRNA. Translation: CAB70738.1.
CCDSiCCDS69513.1. [Q8N6M0-2]
PIRiT46264.
RefSeqiNP_001273674.1. NM_001286745.1. [Q8N6M0-2]
NP_057107.3. NM_016023.3.
UniGeneiHs.30532.

Genome annotation databases

EnsembliENST00000404789; ENSP00000384190; ENSG00000155100.
ENST00000615618; ENSP00000481196; ENSG00000155100. [Q8N6M0-2]
GeneIDi51633.
KEGGihsa:51633.
UCSCiuc003yeu.4. human. [Q8N6M0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151836 mRNA. Translation: AAD34073.1.
AK291646 mRNA. Translation: BAF84335.1.
AK293843 mRNA. Translation: BAG57241.1.
AC087439 Genomic DNA. No translation available.
BC029760 mRNA. Translation: AAH29760.1.
AL137441 mRNA. Translation: CAB70738.1.
CCDSiCCDS69513.1. [Q8N6M0-2]
PIRiT46264.
RefSeqiNP_001273674.1. NM_001286745.1. [Q8N6M0-2]
NP_057107.3. NM_016023.3.
UniGeneiHs.30532.

3D structure databases

ProteinModelPortaliQ8N6M0.
SMRiQ8N6M0. Positions 138-284.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119647. 17 interactions.
IntActiQ8N6M0. 5 interactions.
STRINGi9606.ENSP00000285420.

PTM databases

PhosphoSiteiQ8N6M0.

Polymorphism and mutation databases

BioMutaiOTUD6B.
DMDMi74729149.

Proteomic databases

MaxQBiQ8N6M0.
PaxDbiQ8N6M0.
PRIDEiQ8N6M0.

Protocols and materials databases

DNASUi51633.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000404789; ENSP00000384190; ENSG00000155100.
ENST00000615618; ENSP00000481196; ENSG00000155100. [Q8N6M0-2]
GeneIDi51633.
KEGGihsa:51633.
UCSCiuc003yeu.4. human. [Q8N6M0-1]

Organism-specific databases

CTDi51633.
GeneCardsiGC08P092082.
H-InvDBHIX0201303.
HGNCiHGNC:24281. OTUD6B.
HPAiHPA024046.
HPA024503.
HPA024772.
MIMi612021. gene.
neXtProtiNX_Q8N6M0.
PharmGKBiPA142671219.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5539.
GeneTreeiENSGT00390000012840.
HOGENOMiHOG000192906.
HOVERGENiHBG080227.
InParanoidiQ8N6M0.
KOiK18342.
OrthoDBiEOG7WT42W.
PhylomeDBiQ8N6M0.
TreeFamiTF315010.

Miscellaneous databases

GeneWikiiOTUD6B.
GenomeRNAii51633.
NextBioi35471351.
PROiQ8N6M0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8N6M0.
CleanExiHS_OTUD6B.
ExpressionAtlasiQ8N6M0. baseline and differential.
GenevisibleiQ8N6M0. HS.

Family and domain databases

InterProiIPR003323. OTU_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
    Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
    Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Cerebellum and Placenta.
  3. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lymph.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 67-293 (ISOFORM 1), VARIANT GLN-283.
    Tissue: Amygdala.
  6. "OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis."
    Mevissen T.E., Hospenthal M.K., Geurink P.P., Elliott P.R., Akutsu M., Arnaudo N., Ekkebus R., Kulathu Y., Wauer T., El Oualid F., Freund S.M., Ovaa H., Komander D.
    Cell 154:169-184(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiOTU6B_HUMAN
AccessioniPrimary (citable) accession number: Q8N6M0
Secondary accession number(s): A8K6I1
, B4DEY0, Q9NTA4, Q9Y387
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: October 1, 2002
Last modified: July 22, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

No deubiquitinase activity has been detected when tested (PubMed:23827681). May require some post-translational modification to be active.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.