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Q8N6M0

- OTU6B_HUMAN

UniProt

Q8N6M0 - OTU6B_HUMAN

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Protein

OTU domain-containing protein 6B

Gene
OTUD6B, DUBA5, CGI-77
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei155 – 1551 By similarity
Active sitei158 – 1581Nucleophile By similarity
Active sitei277 – 2771 By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
OTU domain-containing protein 6B (EC:3.4.19.12)
Alternative name(s):
DUBA-5
Gene namesi
Name:OTUD6B
Synonyms:DUBA5
ORF Names:CGI-77
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:24281. OTUD6B.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671219.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 293293OTU domain-containing protein 6BPRO_0000076279Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8N6M0.
PaxDbiQ8N6M0.
PRIDEiQ8N6M0.

PTM databases

PhosphoSiteiQ8N6M0.

Expressioni

Gene expression databases

ArrayExpressiQ8N6M0.
BgeeiQ8N6M0.
CleanExiHS_OTUD6B.
GenevestigatoriQ8N6M0.

Organism-specific databases

HPAiHPA024046.
HPA024503.
HPA024772.

Interactioni

Protein-protein interaction databases

BioGridi119647. 16 interactions.
IntActiQ8N6M0. 5 interactions.
STRINGi9606.ENSP00000285420.

Structurei

3D structure databases

ProteinModelPortaliQ8N6M0.
SMRiQ8N6M0. Positions 138-284.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini147 – 284138OTUAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni152 – 1587Cys-loop By similarity
Regioni219 – 22911Variable-loop By similarityAdd
BLAST
Regioni267 – 27711His-loop By similarityAdd
BLAST

Sequence similaritiesi

Contains 1 OTU domain.

Phylogenomic databases

eggNOGiCOG5539.
HOGENOMiHOG000192906.
HOVERGENiHBG080227.
InParanoidiQ8N6M0.
KOiK18342.
OrthoDBiEOG7WT42W.
PhylomeDBiQ8N6M0.
TreeFamiTF315010.

Family and domain databases

InterProiIPR003323. OTU.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8N6M0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEAVLTEELD EEEQLLRRHR KEKKELQAKI QGMKNAVPKN DKKRRKQLTE    50
DVAKLEKEME QKHREELEQL KLTTKENKID SVAVNISNLV LENQPPRISK 100
AQKRREKKAA LEKEREERIA EAEIENLTGA RHMESEKLAQ ILAARQLEIK 150
QIPSDGHCMY KAIEDQLKEK DCALTVVALR SQTAEYMQSH VEDFLPFLTN 200
PNTGDMYTPE EFQKYCEDIV NTAAWGGQLE LRALSHILQT PIEIIQADSP 250
PIIVGEEYSK KPLILVYMRH AYGLGEHYNS VTRLVNIVTE NCS 293
Length:293
Mass (Da):33,813
Last modified:October 1, 2002 - v1
Checksum:i468DB88E2637D869
GO
Isoform 2 (identifier: Q8N6M0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: MEAVLTEELD...PRISKAQKRR → MISK

Note: No experimental confirmation available.

Show »
Length:192
Mass (Da):21,809
Checksum:iDB3F389CEE3BEB3E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti283 – 2831R → Q.1 Publication
Corresponds to variant rs3210518 [ dbSNP | Ensembl ].
VAR_034144

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 105105MEAVL…AQKRR → MISK in isoform 2. VSP_055378Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti285 – 2939VNIVTENCS → GKHSY in AAD34073. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF151836 mRNA. Translation: AAD34073.1.
AK291646 mRNA. Translation: BAF84335.1.
AK293843 mRNA. Translation: BAG57241.1.
AC087439 Genomic DNA. No translation available.
BC029760 mRNA. Translation: AAH29760.1.
AL137441 mRNA. Translation: CAB70738.1.
PIRiT46264.
RefSeqiNP_001273674.1. NM_001286745.1.
NP_057107.3. NM_016023.3.
UniGeneiHs.30532.

Genome annotation databases

EnsembliENST00000285420; ENSP00000285420; ENSG00000155100.
ENST00000404789; ENSP00000384190; ENSG00000155100.
GeneIDi51633.
KEGGihsa:51633.
UCSCiuc003yeu.4. human. [Q8N6M0-1]

Polymorphism databases

DMDMi74729149.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF151836 mRNA. Translation: AAD34073.1 .
AK291646 mRNA. Translation: BAF84335.1 .
AK293843 mRNA. Translation: BAG57241.1 .
AC087439 Genomic DNA. No translation available.
BC029760 mRNA. Translation: AAH29760.1 .
AL137441 mRNA. Translation: CAB70738.1 .
PIRi T46264.
RefSeqi NP_001273674.1. NM_001286745.1.
NP_057107.3. NM_016023.3.
UniGenei Hs.30532.

3D structure databases

ProteinModelPortali Q8N6M0.
SMRi Q8N6M0. Positions 138-284.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 119647. 16 interactions.
IntActi Q8N6M0. 5 interactions.
STRINGi 9606.ENSP00000285420.

PTM databases

PhosphoSitei Q8N6M0.

Polymorphism databases

DMDMi 74729149.

Proteomic databases

MaxQBi Q8N6M0.
PaxDbi Q8N6M0.
PRIDEi Q8N6M0.

Protocols and materials databases

DNASUi 51633.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000285420 ; ENSP00000285420 ; ENSG00000155100 .
ENST00000404789 ; ENSP00000384190 ; ENSG00000155100 .
GeneIDi 51633.
KEGGi hsa:51633.
UCSCi uc003yeu.4. human. [Q8N6M0-1 ]

Organism-specific databases

CTDi 51633.
GeneCardsi GC08P092082.
H-InvDB HIX0201303.
HGNCi HGNC:24281. OTUD6B.
HPAi HPA024046.
HPA024503.
HPA024772.
MIMi 612021. gene.
neXtProti NX_Q8N6M0.
PharmGKBi PA142671219.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5539.
HOGENOMi HOG000192906.
HOVERGENi HBG080227.
InParanoidi Q8N6M0.
KOi K18342.
OrthoDBi EOG7WT42W.
PhylomeDBi Q8N6M0.
TreeFami TF315010.

Miscellaneous databases

GeneWikii OTUD6B.
GenomeRNAii 51633.
NextBioi 35471351.
PROi Q8N6M0.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8N6M0.
Bgeei Q8N6M0.
CleanExi HS_OTUD6B.
Genevestigatori Q8N6M0.

Family and domain databases

InterProi IPR003323. OTU.
[Graphical view ]
Pfami PF02338. OTU. 1 hit.
[Graphical view ]
PROSITEi PS50802. OTU. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
    Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
    Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Cerebellum and Placenta.
  3. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lymph.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 67-293 (ISOFORM 1), VARIANT GLN-283.
    Tissue: Amygdala.
  6. "OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis."
    Mevissen T.E., Hospenthal M.K., Geurink P.P., Elliott P.R., Akutsu M., Arnaudo N., Ekkebus R., Kulathu Y., Wauer T., El Oualid F., Freund S.M., Ovaa H., Komander D.
    Cell 154:169-184(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiOTU6B_HUMAN
AccessioniPrimary (citable) accession number: Q8N6M0
Secondary accession number(s): A8K6I1
, B4DEY0, Q9NTA4, Q9Y387
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: October 1, 2002
Last modified: September 3, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

No deubiquitinase activity has been detected when tested (1 Publication). May require some post-translational modification to be active.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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