Q8N6I1 (EID2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
EP300-interacting inhibitor of differentiation 2
Short name=EID-2

Alternative name(s):
CREBBP/EP300 inhibitor 2
EID-1-like inhibitor of differentiation 2

Gene names

Name:	EID2
Synonyms:	CRI2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with EP300 and acts as a repressor of MYOD-dependent transcription and muscle differentiation. Inhibits EP300 histone acetyltransferase activity. Acts as a repressor of TGFB/SMAD transcriptional responses. May act as a repressor of the TGFB/SMAD3-dependent signaling by selectively blocking formation of TGFB-induced SMAD3-SMAD4 complex. Ref.1 Ref.5 Ref.6
Subunit structure	Heterodimer with EID2B. Interacts with the C-terminus of EP300. Interacts with HDAC1 and HDAC2. Interacts with SMAD2, SMAD4 and with the MH2 domain of SMAD3. Ref.1 Ref.5 Ref.6 Ref.7
Subcellular location	Nucleus Ref.1.
Tissue specificity	Most abundantly expressed in placenta. Highly expressed in liver, brain, heart, skeletal muscle, and kidney. Ref.1
Domain	The N-terminal portion of EID2 is required for nuclear localization. Ref.1

Ontologies

Keywords
Biological process	Differentiation Myogenesis Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Molecular function	Developmental protein Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	SMAD protein complex assembly Inferred from direct assay Ref.6. Source: HGNC cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW muscle organ development Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.6. Source: HGNC negative regulation of transforming growth factor beta receptor signaling pathway Inferred from direct assay Ref.6. Source: HGNC regulation of cell proliferation Inferred from direct assay Ref.6. Source: HGNC transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transforming growth factor beta receptor complex assembly Inferred from direct assay Ref.6. Source: HGNC
Cellular_component	nucleus Inferred from direct assay. Source: HPA
Molecular_function	SMAD binding Inferred from direct assay Ref.6. Source: HGNC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EID3	Q8N140	3	EBI-5525894,EBI-744483

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 Ref.3 Ref.4 (identifier: Q8N6I1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.2 (identifier: Q8N6I1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     43-91: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	EP300-interacting inhibitor of differentiation 2		PRO_0000315901
Regions
	Coiled coil	170 – 190	21	Potential
	Compositional bias	35 – 109	75	Ala-rich
Natural variations
	Alternative sequence	43 – 91	49	Missing in isoform 2. Ref.2		VSP_052648
	Natural variant	6	1	A → T. Ref.2 Corresponds to variant rs7252027 [ dbSNP | Ensembl ].		VAR_038351
	Natural variant	60	1	E → A. Corresponds to variant rs3746086 [ dbSNP | Ensembl ].		VAR_050964

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 19, 2005. Version 3. Checksum: A69BD5AA098EDC95	FASTA	236	25,190
	10 20 30 40 50 60 MSKLPADSSV PQTGAANGDR DVPQAEVGRG RREPAPAQPE EAGEGAMAAA RGGPVPAARE 70 80 90 100 110 120 GRMAAARAAP AAAARGAPVA AAALARAAAA GRESPAAAAA REARMAEVAR LLGEPVDEEG 130 140 150 160 170 180 PEGRPRSRHG NGGLAALPYL RLRHPLSVLG INYQQFLRHY LENYPIAPGR IQELEERRRR 190 200 210 220 230 FVEACRAREA AFDAEYQRNP HRVDLDILTF TIALTASEVI NPLIEELGCD KFINRE
	Isoform 2 [UniParc]. Checksum: F2A01DD44EFF45B2	FASTA	187	20,856
	10 20 30 40 50 60 MSKLPADSSV PQTGAANGDR DVPQAEVGRG RREPAPAQPE EARESPAAAA AREARMAEVA 70 80 90 100 110 120 RLLGEPVDEE GPEGRPRSRH GNGGLAALPY LRLRHPLSVL GINYQQFLRH YLENYPIAPG 130 140 150 160 170 180 RIQELEERRR RFVEACRARE AAFDAEYQRN PHRVDLDILT FTIALTASEV INPLIEELGC DKFINRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"EID-2, a novel member of the EID family of p300-binding proteins inhibits transactivation by MyoD." Ji A., Dao D., Chen J., MacLellan W.R. Gene 318:35-43(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH EP300, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DOMAIN.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT THR-6. Tissue: Kidney.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung and Skin.
[5]	"A novel EID-1 family member, EID-2, associates with histone deacetylases and inhibits muscle differentiation." Miyake S., Yanagisawa Y., Yuasa Y. J. Biol. Chem. 278:17060-17065(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH HDAC1 AND HDAC2.
[6]	"A novel E1A-like inhibitor of differentiation (EID) family member, EID-2, suppresses transforming growth factor (TGF)-beta signaling by blocking TGF-beta-induced formation of Smad3-Smad4 complexes." Lee H.-J., Lee J.K., Miyake S., Kim S.-J. J. Biol. Chem. 279:2666-2672(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SMAD2; SMAD3 AND SMAD4.
[7]	"A novel EID family member, EID-3, inhibits differentiation and forms a homodimer or heterodimer with EID-2." Sasajima Y., Tanaka H., Miyake S., Yuasa Y. Biochem. Biophys. Res. Commun. 333:969-975(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY251272 mRNA. Translation: AAP12559.1. AK128468 mRNA. Translation: BAC87454.1. CH471126 Genomic DNA. Translation: EAW56909.1. BC030137 mRNA. Translation: AAH30137.3. BC099899 mRNA. Translation: AAH99899.1.
IPI	IPI00879045. IPI00879914.
RefSeq	NP_694964.3. NM_153232.3.
UniGene	Hs.18949.
3D structure databases
ProteinModelPortal	Q8N6I1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N6I1. 4 interactions.
STRING	9606.ENSP00000375073.
PTM databases
PhosphoSite	Q8N6I1.
Polymorphism databases
DMDM	74762532.
Proteomic databases
PaxDb	Q8N6I1.
PRIDE	Q8N6I1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000390658; ENSP00000375073; ENSG00000176396.
GeneID	163126.
KEGG	hsa:163126.
UCSC	uc002oma.3. human.
Organism-specific databases
CTD	163126.
GeneCards	GC19M040028.
H-InvDB	HIX0015123.
HGNC	HGNC:28292. EID2.
HPA	HPA045477.
MIM	609773. gene.
neXtProt	NX_Q8N6I1.
PharmGKB	PA162384544.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG124477.
HOGENOM	HOG000054219.
InParanoid	Q8N6I1.
OMA	EYQRNPQ.
OrthoDB	EOG4G4GS0.
Gene expression databases
Bgee	Q8N6I1.
CleanEx	HS_EID2.
Genevestigator	Q8N6I1.
Family and domain databases
ProtoNet	Search...
Other
GenomeRNAi	163126.
NextBio	88309.
SOURCE	Search...

Entry information

Entry name

EID2_HUMAN

Accession

Primary (citable) accession number: Q8N6I1
Secondary accession number(s): A7YQ71, Q6X7T0, Q6ZR61

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 19, 2005
Last modified:	April 3, 2013
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.