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Protein

EP300-interacting inhibitor of differentiation 2

Gene

EID2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with EP300 and acts as a repressor of MYOD-dependent transcription and muscle differentiation. Inhibits EP300 histone acetyltransferase activity. Acts as a repressor of TGFB/SMAD transcriptional responses. May act as a repressor of the TGFB/SMAD3-dependent signaling by selectively blocking formation of TGFB-induced SMAD3-SMAD4 complex.3 Publications

GO - Molecular functioni

  • SMAD binding Source: HGNC

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • muscle organ development Source: UniProtKB-KW
  • negative regulation of transcription, DNA-templated Source: HGNC
  • negative regulation of transforming growth factor beta receptor signaling pathway Source: HGNC
  • regulation of cell proliferation Source: HGNC
  • regulation of transforming growth factor beta receptor signaling pathway Source: HGNC
  • SMAD protein complex assembly Source: HGNC
  • transcription, DNA-templated Source: UniProtKB-KW
  • transforming growth factor beta receptor complex assembly Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Myogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
EP300-interacting inhibitor of differentiation 2
Short name:
EID-2
Alternative name(s):
CREBBP/EP300 inhibitor 2
EID-1-like inhibitor of differentiation 2
Gene namesi
Name:EID2Imported
Synonyms:CRI2Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:28292. EID2.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • intracellular Source: HGNC
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162384544.

Polymorphism and mutation databases

BioMutaiEID2.
DMDMi74762532.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 236236EP300-interacting inhibitor of differentiation 2PRO_0000315901Add
BLAST

Proteomic databases

MaxQBiQ8N6I1.
PaxDbiQ8N6I1.
PRIDEiQ8N6I1.

PTM databases

PhosphoSiteiQ8N6I1.

Expressioni

Tissue specificityi

Most abundantly expressed in placenta. Highly expressed in liver, brain, heart, skeletal muscle, and kidney.1 Publication

Gene expression databases

BgeeiQ8N6I1.
CleanExiHS_EID2.
GenevisibleiQ8N6I1. HS.

Organism-specific databases

HPAiHPA045477.

Interactioni

Subunit structurei

Heterodimer with EID2B. Interacts with the C-terminus of EP300. Interacts with HDAC1 and HDAC2. Interacts with SMAD2, SMAD4 and with the MH2 domain of SMAD3.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
EID3Q8N1403EBI-5525894,EBI-744483

Protein-protein interaction databases

BioGridi127852. 13 interactions.
IntActiQ8N6I1. 4 interactions.
STRINGi9606.ENSP00000375073.

Structurei

3D structure databases

ProteinModelPortaliQ8N6I1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili170 – 19021Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi35 – 10975Ala-richSequence AnalysisAdd
BLAST

Domaini

The N-terminal portion of EID2 is required for nuclear localization.1 Publication

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG124477.
GeneTreeiENSGT00530000064390.
HOGENOMiHOG000054219.
InParanoidiQ8N6I1.
OMAiEHYPIAP.
OrthoDBiEOG73FQPX.
PhylomeDBiQ8N6I1.
TreeFamiTF337633.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 12 Publications (identifier: Q8N6I1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKLPADSSV PQTGAANGDR DVPQAEVGRG RREPAPAQPE EAGEGAMAAA
60 70 80 90 100
RGGPVPAARE GRMAAARAAP AAAARGAPVA AAALARAAAA GRESPAAAAA
110 120 130 140 150
REARMAEVAR LLGEPVDEEG PEGRPRSRHG NGGLAALPYL RLRHPLSVLG
160 170 180 190 200
INYQQFLRHY LENYPIAPGR IQELEERRRR FVEACRAREA AFDAEYQRNP
210 220 230
HRVDLDILTF TIALTASEVI NPLIEELGCD KFINRE
Length:236
Mass (Da):25,190
Last modified:July 19, 2005 - v3
Checksum:iA69BD5AA098EDC95
GO
Isoform 21 Publication (identifier: Q8N6I1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-91: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:187
Mass (Da):20,856
Checksum:iF2A01DD44EFF45B2
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti6 – 61A → T.1 Publication
Corresponds to variant rs7252027 [ dbSNP | Ensembl ].
VAR_038351
Natural varianti60 – 601E → A.
Corresponds to variant rs3746086 [ dbSNP | Ensembl ].
VAR_050964

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei43 – 9149Missing in isoform 2. 1 PublicationVSP_052648Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY251272 mRNA. Translation: AAP12559.1.
AK128468 mRNA. Translation: BAC87454.1.
CH471126 Genomic DNA. Translation: EAW56909.1.
BC030137 mRNA. Translation: AAH30137.3.
BC099899 mRNA. Translation: AAH99899.1.
CCDSiCCDS12540.2. [Q8N6I1-1]
RefSeqiNP_694964.3. NM_153232.3. [Q8N6I1-1]
UniGeneiHs.18949.

Genome annotation databases

EnsembliENST00000390658; ENSP00000375073; ENSG00000176396.
GeneIDi163126.
KEGGihsa:163126.
UCSCiuc002oma.3. human. [Q8N6I1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY251272 mRNA. Translation: AAP12559.1.
AK128468 mRNA. Translation: BAC87454.1.
CH471126 Genomic DNA. Translation: EAW56909.1.
BC030137 mRNA. Translation: AAH30137.3.
BC099899 mRNA. Translation: AAH99899.1.
CCDSiCCDS12540.2. [Q8N6I1-1]
RefSeqiNP_694964.3. NM_153232.3. [Q8N6I1-1]
UniGeneiHs.18949.

3D structure databases

ProteinModelPortaliQ8N6I1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127852. 13 interactions.
IntActiQ8N6I1. 4 interactions.
STRINGi9606.ENSP00000375073.

PTM databases

PhosphoSiteiQ8N6I1.

Polymorphism and mutation databases

BioMutaiEID2.
DMDMi74762532.

Proteomic databases

MaxQBiQ8N6I1.
PaxDbiQ8N6I1.
PRIDEiQ8N6I1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000390658; ENSP00000375073; ENSG00000176396.
GeneIDi163126.
KEGGihsa:163126.
UCSCiuc002oma.3. human. [Q8N6I1-1]

Organism-specific databases

CTDi163126.
GeneCardsiGC19M040028.
H-InvDBHIX0015123.
HGNCiHGNC:28292. EID2.
HPAiHPA045477.
MIMi609773. gene.
neXtProtiNX_Q8N6I1.
PharmGKBiPA162384544.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG124477.
GeneTreeiENSGT00530000064390.
HOGENOMiHOG000054219.
InParanoidiQ8N6I1.
OMAiEHYPIAP.
OrthoDBiEOG73FQPX.
PhylomeDBiQ8N6I1.
TreeFamiTF337633.

Miscellaneous databases

ChiTaRSiEID2. human.
GenomeRNAii163126.
NextBioi88309.
PROiQ8N6I1.
SOURCEiSearch...

Gene expression databases

BgeeiQ8N6I1.
CleanExiHS_EID2.
GenevisibleiQ8N6I1. HS.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "EID-2, a novel member of the EID family of p300-binding proteins inhibits transactivation by MyoD."
    Ji A., Dao D., Chen J., MacLellan W.R.
    Gene 318:35-43(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH EP300, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DOMAIN.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT THR-6.
    Tissue: KidneyImported.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: LungImported and SkinImported.
  5. "A novel EID-1 family member, EID-2, associates with histone deacetylases and inhibits muscle differentiation."
    Miyake S., Yanagisawa Y., Yuasa Y.
    J. Biol. Chem. 278:17060-17065(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HDAC1 AND HDAC2.
  6. "A novel E1A-like inhibitor of differentiation (EID) family member, EID-2, suppresses transforming growth factor (TGF)-beta signaling by blocking TGF-beta-induced formation of Smad3-Smad4 complexes."
    Lee H.-J., Lee J.K., Miyake S., Kim S.-J.
    J. Biol. Chem. 279:2666-2672(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SMAD2; SMAD3 AND SMAD4.
  7. "A novel EID family member, EID-3, inhibits differentiation and forms a homodimer or heterodimer with EID-2."
    Sasajima Y., Tanaka H., Miyake S., Yuasa Y.
    Biochem. Biophys. Res. Commun. 333:969-975(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiEID2_HUMAN
AccessioniPrimary (citable) accession number: Q8N6I1
Secondary accession number(s): A7YQ71, Q6X7T0, Q6ZR61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 19, 2005
Last modified: July 22, 2015
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.