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Q8N6I1

- EID2_HUMAN

UniProt

Q8N6I1 - EID2_HUMAN

Protein

EP300-interacting inhibitor of differentiation 2

Gene

EID2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 3 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Interacts with EP300 and acts as a repressor of MYOD-dependent transcription and muscle differentiation. Inhibits EP300 histone acetyltransferase activity. Acts as a repressor of TGFB/SMAD transcriptional responses. May act as a repressor of the TGFB/SMAD3-dependent signaling by selectively blocking formation of TGFB-induced SMAD3-SMAD4 complex.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. SMAD binding Source: HGNC

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. muscle organ development Source: UniProtKB-KW
    3. negative regulation of transcription, DNA-templated Source: HGNC
    4. negative regulation of transforming growth factor beta receptor signaling pathway Source: HGNC
    5. regulation of cell proliferation Source: HGNC
    6. regulation of transforming growth factor beta receptor signaling pathway Source: HGNC
    7. SMAD protein complex assembly Source: HGNC
    8. transcription, DNA-templated Source: UniProtKB-KW
    9. transforming growth factor beta receptor complex assembly Source: HGNC

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Differentiation, Myogenesis, Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    EP300-interacting inhibitor of differentiation 2
    Short name:
    EID-2
    Alternative name(s):
    CREBBP/EP300 inhibitor 2
    EID-1-like inhibitor of differentiation 2
    Gene namesi
    Name:EID2Imported
    Synonyms:CRI2Imported
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:28292. EID2.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. intracellular Source: HGNC
    2. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162384544.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 236236EP300-interacting inhibitor of differentiation 2PRO_0000315901Add
    BLAST

    Proteomic databases

    MaxQBiQ8N6I1.
    PaxDbiQ8N6I1.
    PRIDEiQ8N6I1.

    PTM databases

    PhosphoSiteiQ8N6I1.

    Expressioni

    Tissue specificityi

    Most abundantly expressed in placenta. Highly expressed in liver, brain, heart, skeletal muscle, and kidney.1 Publication

    Gene expression databases

    BgeeiQ8N6I1.
    CleanExiHS_EID2.
    GenevestigatoriQ8N6I1.

    Organism-specific databases

    HPAiHPA045477.

    Interactioni

    Subunit structurei

    Heterodimer with EID2B. Interacts with the C-terminus of EP300. Interacts with HDAC1 and HDAC2. Interacts with SMAD2, SMAD4 and with the MH2 domain of SMAD3.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EID3Q8N1403EBI-5525894,EBI-744483

    Protein-protein interaction databases

    BioGridi127852. 14 interactions.
    IntActiQ8N6I1. 4 interactions.
    STRINGi9606.ENSP00000375073.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8N6I1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili170 – 19021Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi35 – 10975Ala-richSequence AnalysisAdd
    BLAST

    Domaini

    The N-terminal portion of EID2 is required for nuclear localization.1 Publication

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG124477.
    HOGENOMiHOG000054219.
    InParanoidiQ8N6I1.
    OMAiPQRVDFD.
    OrthoDBiEOG73FQPX.
    PhylomeDBiQ8N6I1.
    TreeFamiTF337633.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 12 Publications (identifier: Q8N6I1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSKLPADSSV PQTGAANGDR DVPQAEVGRG RREPAPAQPE EAGEGAMAAA    50
    RGGPVPAARE GRMAAARAAP AAAARGAPVA AAALARAAAA GRESPAAAAA 100
    REARMAEVAR LLGEPVDEEG PEGRPRSRHG NGGLAALPYL RLRHPLSVLG 150
    INYQQFLRHY LENYPIAPGR IQELEERRRR FVEACRAREA AFDAEYQRNP 200
    HRVDLDILTF TIALTASEVI NPLIEELGCD KFINRE 236
    Length:236
    Mass (Da):25,190
    Last modified:July 19, 2005 - v3
    Checksum:iA69BD5AA098EDC95
    GO
    Isoform 21 Publication (identifier: Q8N6I1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         43-91: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:187
    Mass (Da):20,856
    Checksum:iF2A01DD44EFF45B2
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti6 – 61A → T.1 Publication
    Corresponds to variant rs7252027 [ dbSNP | Ensembl ].
    VAR_038351
    Natural varianti60 – 601E → A.
    Corresponds to variant rs3746086 [ dbSNP | Ensembl ].
    VAR_050964

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei43 – 9149Missing in isoform 2. 1 PublicationVSP_052648Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY251272 mRNA. Translation: AAP12559.1.
    AK128468 mRNA. Translation: BAC87454.1.
    CH471126 Genomic DNA. Translation: EAW56909.1.
    BC030137 mRNA. Translation: AAH30137.3.
    BC099899 mRNA. Translation: AAH99899.1.
    CCDSiCCDS12540.2. [Q8N6I1-1]
    RefSeqiNP_694964.3. NM_153232.3. [Q8N6I1-1]
    UniGeneiHs.18949.

    Genome annotation databases

    EnsembliENST00000390658; ENSP00000375073; ENSG00000176396. [Q8N6I1-1]
    GeneIDi163126.
    KEGGihsa:163126.
    UCSCiuc002oma.3. human. [Q8N6I1-1]

    Polymorphism databases

    DMDMi74762532.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY251272 mRNA. Translation: AAP12559.1 .
    AK128468 mRNA. Translation: BAC87454.1 .
    CH471126 Genomic DNA. Translation: EAW56909.1 .
    BC030137 mRNA. Translation: AAH30137.3 .
    BC099899 mRNA. Translation: AAH99899.1 .
    CCDSi CCDS12540.2. [Q8N6I1-1 ]
    RefSeqi NP_694964.3. NM_153232.3. [Q8N6I1-1 ]
    UniGenei Hs.18949.

    3D structure databases

    ProteinModelPortali Q8N6I1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 127852. 14 interactions.
    IntActi Q8N6I1. 4 interactions.
    STRINGi 9606.ENSP00000375073.

    PTM databases

    PhosphoSitei Q8N6I1.

    Polymorphism databases

    DMDMi 74762532.

    Proteomic databases

    MaxQBi Q8N6I1.
    PaxDbi Q8N6I1.
    PRIDEi Q8N6I1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000390658 ; ENSP00000375073 ; ENSG00000176396 . [Q8N6I1-1 ]
    GeneIDi 163126.
    KEGGi hsa:163126.
    UCSCi uc002oma.3. human. [Q8N6I1-1 ]

    Organism-specific databases

    CTDi 163126.
    GeneCardsi GC19M040028.
    H-InvDB HIX0015123.
    HGNCi HGNC:28292. EID2.
    HPAi HPA045477.
    MIMi 609773. gene.
    neXtProti NX_Q8N6I1.
    PharmGKBi PA162384544.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG124477.
    HOGENOMi HOG000054219.
    InParanoidi Q8N6I1.
    OMAi PQRVDFD.
    OrthoDBi EOG73FQPX.
    PhylomeDBi Q8N6I1.
    TreeFami TF337633.

    Miscellaneous databases

    GenomeRNAii 163126.
    NextBioi 88309.
    PROi Q8N6I1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8N6I1.
    CleanExi HS_EID2.
    Genevestigatori Q8N6I1.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "EID-2, a novel member of the EID family of p300-binding proteins inhibits transactivation by MyoD."
      Ji A., Dao D., Chen J., MacLellan W.R.
      Gene 318:35-43(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH EP300, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DOMAIN.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT THR-6.
      Tissue: KidneyImported.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: LungImported and SkinImported.
    5. "A novel EID-1 family member, EID-2, associates with histone deacetylases and inhibits muscle differentiation."
      Miyake S., Yanagisawa Y., Yuasa Y.
      J. Biol. Chem. 278:17060-17065(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HDAC1 AND HDAC2.
    6. "A novel E1A-like inhibitor of differentiation (EID) family member, EID-2, suppresses transforming growth factor (TGF)-beta signaling by blocking TGF-beta-induced formation of Smad3-Smad4 complexes."
      Lee H.-J., Lee J.K., Miyake S., Kim S.-J.
      J. Biol. Chem. 279:2666-2672(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SMAD2; SMAD3 AND SMAD4.
    7. "A novel EID family member, EID-3, inhibits differentiation and forms a homodimer or heterodimer with EID-2."
      Sasajima Y., Tanaka H., Miyake S., Yuasa Y.
      Biochem. Biophys. Res. Commun. 333:969-975(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.

    Entry informationi

    Entry nameiEID2_HUMAN
    AccessioniPrimary (citable) accession number: Q8N6I1
    Secondary accession number(s): A7YQ71, Q6X7T0, Q6ZR61
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 84 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3