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Q8N6I1

- EID2_HUMAN

UniProt

Q8N6I1 - EID2_HUMAN

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Protein

EP300-interacting inhibitor of differentiation 2

Gene

EID2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Interacts with EP300 and acts as a repressor of MYOD-dependent transcription and muscle differentiation. Inhibits EP300 histone acetyltransferase activity. Acts as a repressor of TGFB/SMAD transcriptional responses. May act as a repressor of the TGFB/SMAD3-dependent signaling by selectively blocking formation of TGFB-induced SMAD3-SMAD4 complex.3 Publications

GO - Molecular functioni

  1. SMAD binding Source: HGNC

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. muscle organ development Source: UniProtKB-KW
  3. negative regulation of transcription, DNA-templated Source: HGNC
  4. negative regulation of transforming growth factor beta receptor signaling pathway Source: HGNC
  5. regulation of cell proliferation Source: HGNC
  6. regulation of transforming growth factor beta receptor signaling pathway Source: HGNC
  7. SMAD protein complex assembly Source: HGNC
  8. transcription, DNA-templated Source: UniProtKB-KW
  9. transforming growth factor beta receptor complex assembly Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Myogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
EP300-interacting inhibitor of differentiation 2
Short name:
EID-2
Alternative name(s):
CREBBP/EP300 inhibitor 2
EID-1-like inhibitor of differentiation 2
Gene namesi
Name:EID2Imported
Synonyms:CRI2Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:28292. EID2.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. intracellular Source: HGNC
  2. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162384544.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 236236EP300-interacting inhibitor of differentiation 2PRO_0000315901Add
BLAST

Proteomic databases

MaxQBiQ8N6I1.
PaxDbiQ8N6I1.
PRIDEiQ8N6I1.

PTM databases

PhosphoSiteiQ8N6I1.

Expressioni

Tissue specificityi

Most abundantly expressed in placenta. Highly expressed in liver, brain, heart, skeletal muscle, and kidney.1 Publication

Gene expression databases

BgeeiQ8N6I1.
CleanExiHS_EID2.
GenevestigatoriQ8N6I1.

Organism-specific databases

HPAiHPA045477.

Interactioni

Subunit structurei

Heterodimer with EID2B. Interacts with the C-terminus of EP300. Interacts with HDAC1 and HDAC2. Interacts with SMAD2, SMAD4 and with the MH2 domain of SMAD3.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
EID3Q8N1403EBI-5525894,EBI-744483

Protein-protein interaction databases

BioGridi127852. 14 interactions.
IntActiQ8N6I1. 4 interactions.
STRINGi9606.ENSP00000375073.

Structurei

3D structure databases

ProteinModelPortaliQ8N6I1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili170 – 19021Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi35 – 10975Ala-richSequence AnalysisAdd
BLAST

Domaini

The N-terminal portion of EID2 is required for nuclear localization.1 Publication

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG124477.
GeneTreeiENSGT00530000064390.
HOGENOMiHOG000054219.
InParanoidiQ8N6I1.
OMAiPQRVDFD.
OrthoDBiEOG73FQPX.
PhylomeDBiQ8N6I1.
TreeFamiTF337633.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 12 Publications (identifier: Q8N6I1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKLPADSSV PQTGAANGDR DVPQAEVGRG RREPAPAQPE EAGEGAMAAA
60 70 80 90 100
RGGPVPAARE GRMAAARAAP AAAARGAPVA AAALARAAAA GRESPAAAAA
110 120 130 140 150
REARMAEVAR LLGEPVDEEG PEGRPRSRHG NGGLAALPYL RLRHPLSVLG
160 170 180 190 200
INYQQFLRHY LENYPIAPGR IQELEERRRR FVEACRAREA AFDAEYQRNP
210 220 230
HRVDLDILTF TIALTASEVI NPLIEELGCD KFINRE
Length:236
Mass (Da):25,190
Last modified:July 19, 2005 - v3
Checksum:iA69BD5AA098EDC95
GO
Isoform 21 Publication (identifier: Q8N6I1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-91: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:187
Mass (Da):20,856
Checksum:iF2A01DD44EFF45B2
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti6 – 61A → T.1 Publication
Corresponds to variant rs7252027 [ dbSNP | Ensembl ].
VAR_038351
Natural varianti60 – 601E → A.
Corresponds to variant rs3746086 [ dbSNP | Ensembl ].
VAR_050964

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei43 – 9149Missing in isoform 2. 1 PublicationVSP_052648Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY251272 mRNA. Translation: AAP12559.1.
AK128468 mRNA. Translation: BAC87454.1.
CH471126 Genomic DNA. Translation: EAW56909.1.
BC030137 mRNA. Translation: AAH30137.3.
BC099899 mRNA. Translation: AAH99899.1.
CCDSiCCDS12540.2. [Q8N6I1-1]
RefSeqiNP_694964.3. NM_153232.3. [Q8N6I1-1]
UniGeneiHs.18949.

Genome annotation databases

EnsembliENST00000390658; ENSP00000375073; ENSG00000176396. [Q8N6I1-1]
GeneIDi163126.
KEGGihsa:163126.
UCSCiuc002oma.3. human. [Q8N6I1-1]

Polymorphism databases

DMDMi74762532.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY251272 mRNA. Translation: AAP12559.1 .
AK128468 mRNA. Translation: BAC87454.1 .
CH471126 Genomic DNA. Translation: EAW56909.1 .
BC030137 mRNA. Translation: AAH30137.3 .
BC099899 mRNA. Translation: AAH99899.1 .
CCDSi CCDS12540.2. [Q8N6I1-1 ]
RefSeqi NP_694964.3. NM_153232.3. [Q8N6I1-1 ]
UniGenei Hs.18949.

3D structure databases

ProteinModelPortali Q8N6I1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 127852. 14 interactions.
IntActi Q8N6I1. 4 interactions.
STRINGi 9606.ENSP00000375073.

PTM databases

PhosphoSitei Q8N6I1.

Polymorphism databases

DMDMi 74762532.

Proteomic databases

MaxQBi Q8N6I1.
PaxDbi Q8N6I1.
PRIDEi Q8N6I1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000390658 ; ENSP00000375073 ; ENSG00000176396 . [Q8N6I1-1 ]
GeneIDi 163126.
KEGGi hsa:163126.
UCSCi uc002oma.3. human. [Q8N6I1-1 ]

Organism-specific databases

CTDi 163126.
GeneCardsi GC19M040028.
H-InvDB HIX0015123.
HGNCi HGNC:28292. EID2.
HPAi HPA045477.
MIMi 609773. gene.
neXtProti NX_Q8N6I1.
PharmGKBi PA162384544.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG124477.
GeneTreei ENSGT00530000064390.
HOGENOMi HOG000054219.
InParanoidi Q8N6I1.
OMAi PQRVDFD.
OrthoDBi EOG73FQPX.
PhylomeDBi Q8N6I1.
TreeFami TF337633.

Miscellaneous databases

ChiTaRSi EID2. human.
GenomeRNAii 163126.
NextBioi 88309.
PROi Q8N6I1.
SOURCEi Search...

Gene expression databases

Bgeei Q8N6I1.
CleanExi HS_EID2.
Genevestigatori Q8N6I1.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "EID-2, a novel member of the EID family of p300-binding proteins inhibits transactivation by MyoD."
    Ji A., Dao D., Chen J., MacLellan W.R.
    Gene 318:35-43(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH EP300, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DOMAIN.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT THR-6.
    Tissue: KidneyImported.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: LungImported and SkinImported.
  5. "A novel EID-1 family member, EID-2, associates with histone deacetylases and inhibits muscle differentiation."
    Miyake S., Yanagisawa Y., Yuasa Y.
    J. Biol. Chem. 278:17060-17065(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HDAC1 AND HDAC2.
  6. "A novel E1A-like inhibitor of differentiation (EID) family member, EID-2, suppresses transforming growth factor (TGF)-beta signaling by blocking TGF-beta-induced formation of Smad3-Smad4 complexes."
    Lee H.-J., Lee J.K., Miyake S., Kim S.-J.
    J. Biol. Chem. 279:2666-2672(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SMAD2; SMAD3 AND SMAD4.
  7. "A novel EID family member, EID-3, inhibits differentiation and forms a homodimer or heterodimer with EID-2."
    Sasajima Y., Tanaka H., Miyake S., Yuasa Y.
    Biochem. Biophys. Res. Commun. 333:969-975(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiEID2_HUMAN
AccessioniPrimary (citable) accession number: Q8N6I1
Secondary accession number(s): A7YQ71, Q6X7T0, Q6ZR61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 19, 2005
Last modified: November 26, 2014
This is version 86 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3