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Protein

ADP-ribosylation factor GTPase-activating protein 2

Gene

ARFGAP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri26 – 49C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor GTPase-activating protein 2
Short name:
ARF GAP 2
Alternative name(s):
GTPase-activating protein ZNF289
Zinc finger protein 289
Gene namesi
Name:ARFGAP2
Synonyms:ZNF289
ORF Names:Nbla10535
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:13504. ARFGAP2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi84364.
OpenTargetsiENSG00000149182.
PharmGKBiPA162376868.

Polymorphism and mutation databases

BioMutaiARFGAP2.
DMDMi74729129.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002784682 – 521ADP-ribosylation factor GTPase-activating protein 2Add BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei140PhosphoserineCombined sources1
Modified residuei146PhosphoserineCombined sources1
Modified residuei201PhosphoserineCombined sources1
Modified residuei237PhosphoserineCombined sources1
Modified residuei240PhosphoserineCombined sources1
Modified residuei312PhosphoserineCombined sources1
Modified residuei334PhosphoserineCombined sources1
Modified residuei340PhosphoserineCombined sources1
Modified residuei364PhosphoserineCombined sources1
Modified residuei368PhosphoserineCombined sources1
Modified residuei432PhosphoserineCombined sources1
Modified residuei433PhosphoserineCombined sources1
Modified residuei513PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8N6H7.
MaxQBiQ8N6H7.
PaxDbiQ8N6H7.
PeptideAtlasiQ8N6H7.
PRIDEiQ8N6H7.

PTM databases

iPTMnetiQ8N6H7.
PhosphoSitePlusiQ8N6H7.

Expressioni

Gene expression databases

BgeeiENSG00000149182.
CleanExiHS_ARFGAP2.
ExpressionAtlasiQ8N6H7. baseline and differential.
GenevisibleiQ8N6H7. HS.

Organism-specific databases

HPAiHPA016649.
HPA018152.

Interactioni

Subunit structurei

Interacts with the coatomer complex. Interacts with the C-terminal appendage domain of COPG1.2 Publications

Protein-protein interaction databases

BioGridi124065. 17 interactors.
IntActiQ8N6H7. 4 interactors.
STRINGi9606.ENSP00000434442.

Structurei

Secondary structure

1521
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 19Combined sources13
Helixi21 – 24Combined sources4
Turni27 – 29Combined sources3
Beta strandi36 – 38Combined sources3
Turni39 – 42Combined sources4
Beta strandi43 – 45Combined sources3
Helixi47 – 56Combined sources10
Turni58 – 60Combined sources3
Beta strandi63 – 68Combined sources6
Helixi74 – 82Combined sources9
Helixi85 – 94Combined sources10
Helixi102 – 106Combined sources5
Helixi109 – 124Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P57X-ray1.80A3-130[»]
ProteinModelPortaliQ8N6H7.
SMRiQ8N6H7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8N6H7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 127Arf-GAPPROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 521Required for interaction with coatomerAdd BLAST425

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili242 – 308Sequence analysisAdd BLAST67

Sequence similaritiesi

Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri26 – 49C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0706. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00390000005436.
HOGENOMiHOG000162970.
HOVERGENiHBG050563.
InParanoidiQ8N6H7.
KOiK12493.
OMAiAKSPSWA.
OrthoDBiEOG091G0AS9.
PhylomeDBiQ8N6H7.
TreeFamiTF313985.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N6H7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAEPNKTEI QTLFKRLRAV PTNKACFDCG AKNPSWASIT YGVFLCIDCS
60 70 80 90 100
GVHRSLGVHL SFIRSTELDS NWNWFQLRCM QVGGNANATA FFRQHGCTAN
110 120 130 140 150
DANTKYNSRA AQMYREKIRQ LGSAALARHG TDLWIDNMSS AVPNHSPEKK
160 170 180 190 200
DSDFFTEHTQ PPAWDAPATE PSGTQQPAPS TESSGLAQPE HGPNTDLLGT
210 220 230 240 250
SPKASLELKS SIIGKKKPAA AKKGLGAKKG LGAQKVSSQS FSEIERQAQV
260 270 280 290 300
AEKLREQQAA DAKKQAEESM VASMRLAYQE LQIDRKKEEK KLQNLEGKKR
310 320 330 340 350
EQAERLGMGL VSRSSVSHSV LSEMQVIEQE TPVSAKSSRS QLDLFDDVGT
360 370 380 390 400
FASGPPKYKD NPFSLGESFG SRWDTDAAWG MDRVEEKEPE VTISSIRPIS
410 420 430 440 450
ERATNRREVE SRSSGLESSE ARQKFAGAKA ISSDMFFGRE VDAEYEARSR
460 470 480 490 500
LQQLSGSSAI SSSDLFGDMD GAHGAGSVSL GNVLPTADIA QFKQGVKSVA
510 520
GKMAVLANGV MNSLQDRYGS Y
Length:521
Mass (Da):56,720
Last modified:October 1, 2002 - v1
Checksum:i16655EBA94D29E81
GO
Isoform 2 (identifier: Q8N6H7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.
     133-160: Missing.

Note: No experimental confirmation available.
Show »
Length:414
Mass (Da):44,543
Checksum:iCB86DEEF636E8D91
GO
Isoform 3 (identifier: Q8N6H7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-269: Missing.

Note: No experimental confirmation available.
Show »
Length:252
Mass (Da):27,535
Checksum:i8DFD0EE52D062600
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40T → M in BAB55144 (PubMed:14702039).Curated1
Sequence conflicti107N → S in BAB55144 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048321143P → R.Corresponds to variant rs11542793dbSNPEnsembl.1
Natural variantiVAR_048322339R → H.Corresponds to variant rs34662994dbSNPEnsembl.1
Natural variantiVAR_048323406R → W.Corresponds to variant rs35950498dbSNPEnsembl.1
Natural variantiVAR_030780411S → N.1 PublicationCorresponds to variant rs3740691dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0560541 – 269Missing in isoform 3. 1 PublicationAdd BLAST269
Alternative sequenceiVSP_0560551 – 79Missing in isoform 2. 1 PublicationAdd BLAST79
Alternative sequenceiVSP_056056133 – 160Missing in isoform 2. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027482 mRNA. Translation: BAB55144.1.
AK301785 mRNA. Translation: BAG63241.1.
AK315992 mRNA. Translation: BAH14363.1.
AC090589 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW67963.1.
CH471064 Genomic DNA. Translation: EAW67968.1.
BC030148 mRNA. Translation: AAH30148.1.
AL834337 mRNA. Translation: CAD39004.1.
AB073358 mRNA. Translation: BAE45716.1.
CCDSiCCDS7926.1. [Q8N6H7-1]
RefSeqiNP_001229761.1. NM_001242832.1.
NP_115765.2. NM_032389.4. [Q8N6H7-1]
UniGeneiHs.436204.

Genome annotation databases

EnsembliENST00000524782; ENSP00000434442; ENSG00000149182. [Q8N6H7-1]
GeneIDi84364.
KEGGihsa:84364.
UCSCiuc001ndt.4. human. [Q8N6H7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027482 mRNA. Translation: BAB55144.1.
AK301785 mRNA. Translation: BAG63241.1.
AK315992 mRNA. Translation: BAH14363.1.
AC090589 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW67963.1.
CH471064 Genomic DNA. Translation: EAW67968.1.
BC030148 mRNA. Translation: AAH30148.1.
AL834337 mRNA. Translation: CAD39004.1.
AB073358 mRNA. Translation: BAE45716.1.
CCDSiCCDS7926.1. [Q8N6H7-1]
RefSeqiNP_001229761.1. NM_001242832.1.
NP_115765.2. NM_032389.4. [Q8N6H7-1]
UniGeneiHs.436204.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P57X-ray1.80A3-130[»]
ProteinModelPortaliQ8N6H7.
SMRiQ8N6H7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124065. 17 interactors.
IntActiQ8N6H7. 4 interactors.
STRINGi9606.ENSP00000434442.

PTM databases

iPTMnetiQ8N6H7.
PhosphoSitePlusiQ8N6H7.

Polymorphism and mutation databases

BioMutaiARFGAP2.
DMDMi74729129.

Proteomic databases

EPDiQ8N6H7.
MaxQBiQ8N6H7.
PaxDbiQ8N6H7.
PeptideAtlasiQ8N6H7.
PRIDEiQ8N6H7.

Protocols and materials databases

DNASUi84364.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000524782; ENSP00000434442; ENSG00000149182. [Q8N6H7-1]
GeneIDi84364.
KEGGihsa:84364.
UCSCiuc001ndt.4. human. [Q8N6H7-1]

Organism-specific databases

CTDi84364.
DisGeNETi84364.
GeneCardsiARFGAP2.
HGNCiHGNC:13504. ARFGAP2.
HPAiHPA016649.
HPA018152.
MIMi606908. gene.
neXtProtiNX_Q8N6H7.
OpenTargetsiENSG00000149182.
PharmGKBiPA162376868.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0706. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00390000005436.
HOGENOMiHOG000162970.
HOVERGENiHBG050563.
InParanoidiQ8N6H7.
KOiK12493.
OMAiAKSPSWA.
OrthoDBiEOG091G0AS9.
PhylomeDBiQ8N6H7.
TreeFamiTF313985.

Enzyme and pathway databases

ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

ChiTaRSiARFGAP2. human.
EvolutionaryTraceiQ8N6H7.
GenomeRNAii84364.
PROiQ8N6H7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000149182.
CleanExiHS_ARFGAP2.
ExpressionAtlasiQ8N6H7. baseline and differential.
GenevisibleiQ8N6H7. HS.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARFG2_HUMAN
AccessioniPrimary (citable) accession number: Q8N6H7
Secondary accession number(s): B4DX29
, B7Z9M7, D3DQQ9, Q3LIF2, Q8N3I1, Q96SX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Vero cells overexpressing truncated ARFGAP2 show accumulation of cholera toxin A subunit in the Golgi complex rather than the endoplasmic reticulum.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.