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Q8N6G5

- CGAT2_HUMAN

UniProt

Q8N6G5 - CGAT2_HUMAN

Protein

Chondroitin sulfate N-acetylgalactosaminyltransferase 2

Gene

CSGALNACT2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA). Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains.2 Publications

    Catalytic activityi

    UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan = UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi369 – 3691Divalent metal cationSequence Analysis
    Metal bindingi486 – 4861Divalent metal cationSequence Analysis

    GO - Molecular functioni

    1. acetylgalactosaminyltransferase activity Source: UniProtKB
    2. glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate metabolic process Source: Reactome
    2. chondroitin sulfate biosynthetic process Source: Reactome
    3. chondroitin sulfate metabolic process Source: Reactome
    4. chondroitin sulfate proteoglycan biosynthetic process Source: UniProtKB
    5. chondroitin sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: UniProtKB
    6. dermatan sulfate proteoglycan biosynthetic process Source: UniProtKB
    7. dermatan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: UniProtKB
    8. glycosaminoglycan metabolic process Source: Reactome
    9. proteoglycan biosynthetic process Source: UniProtKB
    10. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS10013-MONOMER.
    BRENDAi2.4.1.174. 2681.
    ReactomeiREACT_120989. Chondroitin sulfate biosynthesis.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chondroitin sulfate N-acetylgalactosaminyltransferase 2 (EC:2.4.1.174)
    Alternative name(s):
    Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 2
    Short name:
    Beta4GalNAcT-2
    Short name:
    GalNAcT-2
    Gene namesi
    Name:CSGALNACT2
    Synonyms:CHGN2, GALNACT2
    ORF Names:PRO0082
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 10

    Organism-specific databases

    HGNCiHGNC:24292. CSGALNACT2.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi cisterna membrane Source: UniProtKB-SubCell
    2. Golgi membrane Source: Reactome
    3. integral component of Golgi membrane Source: UniProtKB
    4. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162382854.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 542542Chondroitin sulfate N-acetylgalactosaminyltransferase 2PRO_0000189566Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi41 – 411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ8N6G5.
    PaxDbiQ8N6G5.
    PRIDEiQ8N6G5.

    Expressioni

    Tissue specificityi

    Ubiquitous.2 Publications

    Gene expression databases

    BgeeiQ8N6G5.
    CleanExiHS_CSGALNACT2.
    GenevestigatoriQ8N6G5.

    Organism-specific databases

    HPAiHPA031539.
    HPA031540.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000363590.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8N6G5.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini33 – 542510LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili59 – 10547Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG295515.
    HOGENOMiHOG000294238.
    HOVERGENiHBG050930.
    InParanoidiQ8N6G5.
    KOiK00746.
    OMAiNVGARAW.
    OrthoDBiEOG75TMBN.
    PhylomeDBiQ8N6G5.
    TreeFamiTF318303.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR008428. Chond_GalNAc.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR12369. PTHR12369. 1 hit.
    PfamiPF05679. CHGN. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8N6G5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPRRGLILHT RTHWLLLGLA LLCSLVLFMY LLECAPQTDG NASLPGVVGE    50
    NYGKEYYQAL LQEQEEHYQT RATSLKRQIA QLKQELQEMS EKMRSLQERR 100
    NVGANGIGYQ SNKEQAPSDL LEFLHSQIDK AEVSIGAKLP SEYGVIPFES 150
    FTLMKVFQLE MGLTRHPEEK PVRKDKRDEL VEVIEAGLEV INNPDEDDEQ 200
    EDEEGPLGEK LIFNENDFVE GYYRTERDKG TQYELFFKKA DLTEYRHVTL 250
    FRPFGPLMKV KSEMIDITRS IINIIVPLAE RTEAFVQFMQ NFRDVCIHQD 300
    KKIHLTVVYF GKEGLSKVKS ILESVTSESN FHNYTLVSLN EEFNRGRGLN 350
    VGARAWDKGE VLMFFCDVDI YFSAEFLNSC RLNAEPGKKV FYPVVFSLYN 400
    PAIVYANQEV PPPVEQQLVH KKDSGFWRDF GFGMTCQYRS DFLTIGGFDM 450
    EVKGWGGEDV HLYRKYLHGD LIVIRTPVPG LFHLWHEKRC ADELTPEQYR 500
    MCIQSKAMNE ASHSHLGMLV FREEIETHLH KQAYRTNSEA VG 542
    Length:542
    Mass (Da):62,572
    Last modified:October 1, 2002 - v1
    Checksum:iE19103EE6F903AB6
    GO
    Isoform 2 (identifier: Q8N6G5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         327-333: SESNFHN → RLASSTW
         334-542: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:333
    Mass (Da):38,440
    Checksum:iC802C5BB271F54FB
    GO

    Sequence cautioni

    The sequence AAF71068.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti438 – 4381Y → H in BAB85092. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti215 – 2151E → K.
    Corresponds to variant rs11238456 [ dbSNP | Ensembl ].
    VAR_048715
    Natural varianti479 – 4791P → S.1 Publication
    Corresponds to variant rs2435381 [ dbSNP | Ensembl ].
    VAR_048716

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei327 – 3337SESNFHN → RLASSTW in isoform 2. 1 PublicationVSP_012729
    Alternative sequencei334 – 542209Missing in isoform 2. 1 PublicationVSP_012730Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB079252 mRNA. Translation: BAC55935.1.
    AB090811 mRNA. Translation: BAC55936.1.
    AK074474 mRNA. Translation: BAB85092.1.
    AK125300 mRNA. Translation: BAG54179.1.
    BX640967 mRNA. Translation: CAE45982.1.
    BX641073 mRNA. Translation: CAE46036.1.
    CH471160 Genomic DNA. Translation: EAW86587.1.
    BC030268 mRNA. Translation: AAH30268.1.
    AF116646 mRNA. Translation: AAF71068.1. Different initiation.
    CCDSiCCDS7201.1. [Q8N6G5-1]
    RefSeqiNP_061060.3. NM_018590.4. [Q8N6G5-1]
    XP_005271877.1. XM_005271820.2. [Q8N6G5-2]
    UniGeneiHs.657569.
    Hs.744559.

    Genome annotation databases

    EnsembliENST00000374466; ENSP00000363590; ENSG00000169826. [Q8N6G5-1]
    GeneIDi55454.
    KEGGihsa:55454.
    UCSCiuc001jam.2. human. [Q8N6G5-2]
    uc001jan.4. human. [Q8N6G5-1]

    Polymorphism databases

    DMDMi60391915.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    Functional Glycomics Gateway - GTase

    Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 2

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB079252 mRNA. Translation: BAC55935.1 .
    AB090811 mRNA. Translation: BAC55936.1 .
    AK074474 mRNA. Translation: BAB85092.1 .
    AK125300 mRNA. Translation: BAG54179.1 .
    BX640967 mRNA. Translation: CAE45982.1 .
    BX641073 mRNA. Translation: CAE46036.1 .
    CH471160 Genomic DNA. Translation: EAW86587.1 .
    BC030268 mRNA. Translation: AAH30268.1 .
    AF116646 mRNA. Translation: AAF71068.1 . Different initiation.
    CCDSi CCDS7201.1. [Q8N6G5-1 ]
    RefSeqi NP_061060.3. NM_018590.4. [Q8N6G5-1 ]
    XP_005271877.1. XM_005271820.2. [Q8N6G5-2 ]
    UniGenei Hs.657569.
    Hs.744559.

    3D structure databases

    ProteinModelPortali Q8N6G5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000363590.

    Protein family/group databases

    CAZyi GT7. Glycosyltransferase Family 7.

    Polymorphism databases

    DMDMi 60391915.

    Proteomic databases

    MaxQBi Q8N6G5.
    PaxDbi Q8N6G5.
    PRIDEi Q8N6G5.

    Protocols and materials databases

    DNASUi 55454.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000374466 ; ENSP00000363590 ; ENSG00000169826 . [Q8N6G5-1 ]
    GeneIDi 55454.
    KEGGi hsa:55454.
    UCSCi uc001jam.2. human. [Q8N6G5-2 ]
    uc001jan.4. human. [Q8N6G5-1 ]

    Organism-specific databases

    CTDi 55454.
    GeneCardsi GC10P043633.
    HGNCi HGNC:24292. CSGALNACT2.
    HPAi HPA031539.
    HPA031540.
    neXtProti NX_Q8N6G5.
    PharmGKBi PA162382854.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG295515.
    HOGENOMi HOG000294238.
    HOVERGENi HBG050930.
    InParanoidi Q8N6G5.
    KOi K00746.
    OMAi NVGARAW.
    OrthoDBi EOG75TMBN.
    PhylomeDBi Q8N6G5.
    TreeFami TF318303.

    Enzyme and pathway databases

    BioCyci MetaCyc:HS10013-MONOMER.
    BRENDAi 2.4.1.174. 2681.
    Reactomei REACT_120989. Chondroitin sulfate biosynthesis.

    Miscellaneous databases

    ChiTaRSi CSGALNACT2. human.
    GenomeRNAii 55454.
    NextBioi 59849.
    PROi Q8N6G5.

    Gene expression databases

    Bgeei Q8N6G5.
    CleanExi HS_CSGALNACT2.
    Genevestigatori Q8N6G5.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR008428. Chond_GalNAc.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR12369. PTHR12369. 1 hit.
    Pfami PF05679. CHGN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Differential roles of two N-acetylgalactosaminyltransferases, CSGalNAcT-1, and a novel enzyme, CSGalNAcT-2. Initiation and elongation in synthesis of chondroitin sulfate."
      Sato T., Gotoh M., Kiyohara K., Akashima T., Iwasaki H., Kameyama A., Mochizuki H., Yada T., Inaba N., Togayachi A., Kudo T., Asada M., Watanabe H., Imamura T., Kimata K., Narimatsu H.
      J. Biol. Chem. 278:3063-3071(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    2. "Molecular cloning and expression of a second chondroitin N-acetylgalactosaminyltransferase involved in the initiation and elongation of chondroitin/dermatan sulfate."
      Uyama T., Kitagawa H., Tanaka J., Tamura J., Ogawa T., Sugahara K.
      J. Biol. Chem. 278:3072-3078(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lung.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT SER-479.
      Tissue: Endothelial cell and Small intestine.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Pancreas.
    7. "Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs."
      Yu Y., Zhang C., Zhou G., Wu S., Qu X., Wei H., Xing G., Dong C., Zhai Y., Wan J., Ouyang S., Li L., Zhang S., Zhou K., Zhang Y., Wu C., He F.
      Genome Res. 11:1392-1403(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 418-542 (ISOFORM 1).
      Tissue: Fetal liver.

    Entry informationi

    Entry nameiCGAT2_HUMAN
    AccessioniPrimary (citable) accession number: Q8N6G5
    Secondary accession number(s): B3KWL7
    , Q6MZJ5, Q6MZP6, Q8TCH4, Q9P1I6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 10
      Human chromosome 10: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3