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Protein

Chondroitin sulfate N-acetylgalactosaminyltransferase 2

Gene

CSGALNACT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA). Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains.2 Publications

Catalytic activityi

UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan = UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi369 – 3691Divalent metal cationSequence Analysis
Metal bindingi486 – 4861Divalent metal cationSequence Analysis

GO - Molecular functioni

  1. acetylgalactosaminyltransferase activity Source: UniProtKB
  2. glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: Reactome
  2. chondroitin sulfate biosynthetic process Source: Reactome
  3. chondroitin sulfate metabolic process Source: Reactome
  4. chondroitin sulfate proteoglycan biosynthetic process Source: UniProtKB
  5. chondroitin sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: UniProtKB
  6. dermatan sulfate proteoglycan biosynthetic process Source: UniProtKB
  7. dermatan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: UniProtKB
  8. glycosaminoglycan metabolic process Source: Reactome
  9. pathogenesis Source: Reactome
  10. proteoglycan biosynthetic process Source: UniProtKB
  11. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS10013-MONOMER.
BRENDAi2.4.1.174. 2681.
2.4.1.175. 2681.
ReactomeiREACT_120989. Chondroitin sulfate biosynthesis.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Chondroitin sulfate N-acetylgalactosaminyltransferase 2 (EC:2.4.1.174)
Alternative name(s):
Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 2
Short name:
Beta4GalNAcT-2
Short name:
GalNAcT-2
Gene namesi
Name:CSGALNACT2
Synonyms:CHGN2, GALNACT2
ORF Names:PRO0082
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:24292. CSGALNACT2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei12 – 3221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini33 – 542510LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi cisterna membrane Source: UniProtKB-SubCell
  2. Golgi membrane Source: Reactome
  3. integral component of Golgi membrane Source: UniProtKB
  4. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162382854.

Polymorphism and mutation databases

DMDMi60391915.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 542542Chondroitin sulfate N-acetylgalactosaminyltransferase 2PRO_0000189566Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi41 – 411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8N6G5.
PaxDbiQ8N6G5.
PRIDEiQ8N6G5.

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Gene expression databases

BgeeiQ8N6G5.
CleanExiHS_CSGALNACT2.
GenevestigatoriQ8N6G5.

Organism-specific databases

HPAiHPA031539.
HPA031540.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SYNE4Q8N2053EBI-10267100,EBI-7131783

Protein-protein interaction databases

BioGridi120672. 11 interactions.
IntActiQ8N6G5. 1 interaction.
STRINGi9606.ENSP00000363590.

Structurei

3D structure databases

ProteinModelPortaliQ8N6G5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili59 – 10547Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG295515.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000294238.
HOVERGENiHBG050930.
InParanoidiQ8N6G5.
KOiK00746.
OMAiNVGARAW.
OrthoDBiEOG75TMBN.
PhylomeDBiQ8N6G5.
TreeFamiTF318303.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR008428. Chond_GalNAc.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N6G5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPRRGLILHT RTHWLLLGLA LLCSLVLFMY LLECAPQTDG NASLPGVVGE
60 70 80 90 100
NYGKEYYQAL LQEQEEHYQT RATSLKRQIA QLKQELQEMS EKMRSLQERR
110 120 130 140 150
NVGANGIGYQ SNKEQAPSDL LEFLHSQIDK AEVSIGAKLP SEYGVIPFES
160 170 180 190 200
FTLMKVFQLE MGLTRHPEEK PVRKDKRDEL VEVIEAGLEV INNPDEDDEQ
210 220 230 240 250
EDEEGPLGEK LIFNENDFVE GYYRTERDKG TQYELFFKKA DLTEYRHVTL
260 270 280 290 300
FRPFGPLMKV KSEMIDITRS IINIIVPLAE RTEAFVQFMQ NFRDVCIHQD
310 320 330 340 350
KKIHLTVVYF GKEGLSKVKS ILESVTSESN FHNYTLVSLN EEFNRGRGLN
360 370 380 390 400
VGARAWDKGE VLMFFCDVDI YFSAEFLNSC RLNAEPGKKV FYPVVFSLYN
410 420 430 440 450
PAIVYANQEV PPPVEQQLVH KKDSGFWRDF GFGMTCQYRS DFLTIGGFDM
460 470 480 490 500
EVKGWGGEDV HLYRKYLHGD LIVIRTPVPG LFHLWHEKRC ADELTPEQYR
510 520 530 540
MCIQSKAMNE ASHSHLGMLV FREEIETHLH KQAYRTNSEA VG
Length:542
Mass (Da):62,572
Last modified:October 1, 2002 - v1
Checksum:iE19103EE6F903AB6
GO
Isoform 2 (identifier: Q8N6G5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     327-333: SESNFHN → RLASSTW
     334-542: Missing.

Note: No experimental confirmation available.

Show »
Length:333
Mass (Da):38,440
Checksum:iC802C5BB271F54FB
GO

Sequence cautioni

The sequence AAF71068.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti438 – 4381Y → H in BAB85092 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti215 – 2151E → K.
Corresponds to variant rs11238456 [ dbSNP | Ensembl ].
VAR_048715
Natural varianti479 – 4791P → S.1 Publication
Corresponds to variant rs2435381 [ dbSNP | Ensembl ].
VAR_048716

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei327 – 3337SESNFHN → RLASSTW in isoform 2. 1 PublicationVSP_012729
Alternative sequencei334 – 542209Missing in isoform 2. 1 PublicationVSP_012730Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB079252 mRNA. Translation: BAC55935.1.
AB090811 mRNA. Translation: BAC55936.1.
AK074474 mRNA. Translation: BAB85092.1.
AK125300 mRNA. Translation: BAG54179.1.
BX640967 mRNA. Translation: CAE45982.1.
BX641073 mRNA. Translation: CAE46036.1.
CH471160 Genomic DNA. Translation: EAW86587.1.
BC030268 mRNA. Translation: AAH30268.1.
AF116646 mRNA. Translation: AAF71068.1. Different initiation.
CCDSiCCDS7201.1. [Q8N6G5-1]
RefSeqiNP_061060.3. NM_018590.4. [Q8N6G5-1]
UniGeneiHs.657569.
Hs.744559.

Genome annotation databases

EnsembliENST00000374466; ENSP00000363590; ENSG00000169826. [Q8N6G5-1]
GeneIDi55454.
KEGGihsa:55454.
UCSCiuc001jam.2. human. [Q8N6G5-2]
uc001jan.4. human. [Q8N6G5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB079252 mRNA. Translation: BAC55935.1.
AB090811 mRNA. Translation: BAC55936.1.
AK074474 mRNA. Translation: BAB85092.1.
AK125300 mRNA. Translation: BAG54179.1.
BX640967 mRNA. Translation: CAE45982.1.
BX641073 mRNA. Translation: CAE46036.1.
CH471160 Genomic DNA. Translation: EAW86587.1.
BC030268 mRNA. Translation: AAH30268.1.
AF116646 mRNA. Translation: AAF71068.1. Different initiation.
CCDSiCCDS7201.1. [Q8N6G5-1]
RefSeqiNP_061060.3. NM_018590.4. [Q8N6G5-1]
UniGeneiHs.657569.
Hs.744559.

3D structure databases

ProteinModelPortaliQ8N6G5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120672. 11 interactions.
IntActiQ8N6G5. 1 interaction.
STRINGi9606.ENSP00000363590.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Polymorphism and mutation databases

DMDMi60391915.

Proteomic databases

MaxQBiQ8N6G5.
PaxDbiQ8N6G5.
PRIDEiQ8N6G5.

Protocols and materials databases

DNASUi55454.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000374466; ENSP00000363590; ENSG00000169826. [Q8N6G5-1]
GeneIDi55454.
KEGGihsa:55454.
UCSCiuc001jam.2. human. [Q8N6G5-2]
uc001jan.4. human. [Q8N6G5-1]

Organism-specific databases

CTDi55454.
GeneCardsiGC10P043633.
HGNCiHGNC:24292. CSGALNACT2.
HPAiHPA031539.
HPA031540.
neXtProtiNX_Q8N6G5.
PharmGKBiPA162382854.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG295515.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000294238.
HOVERGENiHBG050930.
InParanoidiQ8N6G5.
KOiK00746.
OMAiNVGARAW.
OrthoDBiEOG75TMBN.
PhylomeDBiQ8N6G5.
TreeFamiTF318303.

Enzyme and pathway databases

BioCyciMetaCyc:HS10013-MONOMER.
BRENDAi2.4.1.174. 2681.
2.4.1.175. 2681.
ReactomeiREACT_120989. Chondroitin sulfate biosynthesis.

Miscellaneous databases

ChiTaRSiCSGALNACT2. human.
GenomeRNAii55454.
NextBioi59849.
PROiQ8N6G5.

Gene expression databases

BgeeiQ8N6G5.
CleanExiHS_CSGALNACT2.
GenevestigatoriQ8N6G5.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR008428. Chond_GalNAc.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Differential roles of two N-acetylgalactosaminyltransferases, CSGalNAcT-1, and a novel enzyme, CSGalNAcT-2. Initiation and elongation in synthesis of chondroitin sulfate."
    Sato T., Gotoh M., Kiyohara K., Akashima T., Iwasaki H., Kameyama A., Mochizuki H., Yada T., Inaba N., Togayachi A., Kudo T., Asada M., Watanabe H., Imamura T., Kimata K., Narimatsu H.
    J. Biol. Chem. 278:3063-3071(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
  2. "Molecular cloning and expression of a second chondroitin N-acetylgalactosaminyltransferase involved in the initiation and elongation of chondroitin/dermatan sulfate."
    Uyama T., Kitagawa H., Tanaka J., Tamura J., Ogawa T., Sugahara K.
    J. Biol. Chem. 278:3072-3078(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT SER-479.
    Tissue: Endothelial cell and Small intestine.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Pancreas.
  7. "Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs."
    Yu Y., Zhang C., Zhou G., Wu S., Qu X., Wei H., Xing G., Dong C., Zhai Y., Wan J., Ouyang S., Li L., Zhang S., Zhou K., Zhang Y., Wu C., He F.
    Genome Res. 11:1392-1403(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 418-542 (ISOFORM 1).
    Tissue: Fetal liver.

Entry informationi

Entry nameiCGAT2_HUMAN
AccessioniPrimary (citable) accession number: Q8N6G5
Secondary accession number(s): B3KWL7
, Q6MZJ5, Q6MZP6, Q8TCH4, Q9P1I6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: October 1, 2002
Last modified: April 29, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.