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Q8N697 (S15A4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 15 member 4
Alternative name(s):
Peptide transporter 4
Peptide/histidine transporter 1
Short name=hPHT1
Gene names
Name:SLC15A4
Synonyms:PHT1, PTR4
ORF Names:FP12591
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length577 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Proton oligopeptide cotransporter. Transports free histidine and certain di- and tripeptides. Ref.9

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Highly expressed in skeletal muscle. Moderately expressed in kidney, liver, and heart. Weakly expressed in colon and brain. Expressed in low levels throughout the gastrointestinal tract and in Caco-2 cells. Expressed in retinal fragment epithelium (RPE) and neural retina. Expressed in small intestine, stomach, duodenum, jejunum, ileum and colon. Ref.7 Ref.8 Ref.9

Sequence similarities

Belongs to the PTR2/POT transporter (TC 2.A.17) family. [View classification]

Sequence caution

The sequence AAI46804.1 differs from that shown. Reason: Frameshift at position 342.

The sequence AAK95565.1 differs from that shown. Reason: Frameshift at position 572.

The sequence AAQ04807.1 differs from that shown. Reason: Frameshift at position 497.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8N697-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8N697-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-337: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 577577Solute carrier family 15 member 4
PRO_0000338599

Regions

Transmembrane49 – 6921Helical; Potential
Transmembrane76 – 9621Helical; Potential
Transmembrane105 – 12521Helical; Potential
Transmembrane158 – 17821Helical; Potential
Transmembrane194 – 21421Helical; Potential
Transmembrane222 – 24221Helical; Potential
Transmembrane318 – 33821Helical; Potential
Transmembrane363 – 38321Helical; Potential
Transmembrane405 – 42521Helical; Potential
Transmembrane459 – 47921Helical; Potential
Transmembrane490 – 51021Helical; Potential
Transmembrane535 – 55521Helical; Potential
Compositional bias8 – 3932Ala-rich

Amino acid modifications

Modified residue2791Phosphoserine By similarity
Modified residue2981Phosphoserine By similarity

Natural variations

Alternative sequence1 – 337337Missing in isoform 2.
VSP_034052
Natural variant2391V → A.
Corresponds to variant rs33990080 [ dbSNP | Ensembl ].
VAR_051611

Experimental info

Sequence conflict241A → T in AAI46804. Ref.6
Sequence conflict34 – 352RA → LL in AAK95565. Ref.1
Sequence conflict441E → G in AAI46804. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 701111038C752E70

FASTA57762,034
        10         20         30         40         50         60 
MEGSGGGAGE RAPLLGARRA AAAAAAAGAF AGRRAACGAV LLTELLERAA FYGITSNLVL 

        70         80         90        100        110        120 
FLNGAPFCWE GAQASEALLL FMGLTYLGSP FGGWLADARL GRARAILLSL ALYLLGMLAF 

       130        140        150        160        170        180 
PLLAAPATRA ALCGSARLLN CTAPGPDAAA RCCSPATFAG LVLVGLGVAT VKANITPFGA 

       190        200        210        220        230        240 
DQVKDRGPEA TRRFFNWFYW SINLGAILSL GGIAYIQQNV SFVTGYAIPT VCVGLAFVVF 

       250        260        270        280        290        300 
LCGQSVFITK PPDGSAFTDM FKILTYSCCS QKRSGERQSN GEGIGVFQQS SKQSLFDSCK 

       310        320        330        340        350        360 
MSHGGPFTEE KVEDVKALVK IVPVFLALIP YWTVYFQMQT TYVLQSLHLR IPEISNITTT 

       370        380        390        400        410        420 
PHTLPAAWLT MFDAVLILLL IPLKDKLVDP ILRRHGLLPS SLKRIAVGMF FVMCSAFAAG 

       430        440        450        460        470        480 
ILESKRLNLV KEKTINQTIG NVVYHAADLS LWWQVPQYLL IGISEIFASI AGLEFAYSAA 

       490        500        510        520        530        540 
PKSMQSAIMG LFFFFSGVGS FVGSGLLALV SIKAIGWMSS HTDFGNINGC YLNYYFFLLA 

       550        560        570 
AIQGATLLLF LIISVKYDHH RDHQRSRANG VPTSRRA 

« Hide

Isoform 2 [UniParc].

Checksum: 2896131C1AB96FB0
Show »

FASTA24026,606

References

« Hide 'large scale' references
[1]"Cloning of a human novel gene with product of peptide/histidine."
Guo J.H., Yu L.
Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Molecular cloning of a human brain peptide/histidine transporter, PTR4."
Mondon P., Bouayadi K., Fournier J., Saubusse P., Lablie C.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"Large-scale cDNA transfection screening for genes related to cancer development and progression."
Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. expand/collapse author list , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[7]"Human proton/oligopeptide transporter (POT) genes: identification of putative human genes using bioinformatics."
Botka C.W., Wittig T.W., Graul R.C., Nielsen C.U., Higaki K., Amidon G.L., Sadee W.
AAPS PharmSci 2:E16-E16(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[8]"Spatial expression patterns of peptide transporters in the human and rat gastrointestinal tracts, Caco-2 in vitro cell culture model, and multiple human tissues."
Herrera-Ruiz D., Wang Q., Gudmundsson O.S., Cook T.J., Smith R.L., Faria T.N., Knipp G.T.
AAPS PharmSci 3:E9-E9(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[9]"The functional evaluation of human peptide/histidine transporter 1 (hPHT1) in transiently transfected COS-7 cells."
Bhardwaj R.K., Herrera-Ruiz D., Eltoukhy N., Saad M., Knipp G.T.
Eur. J. Pharm. Sci. 27:533-542(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY050629 mRNA. Translation: AAK95565.1. Frameshift.
AY038999 mRNA. Translation: AAK72099.1.
AF461893 mRNA. Translation: AAQ04807.1. Frameshift.
AK095717 mRNA. Translation: BAG53113.1.
CH471054 Genomic DNA. Translation: EAW98494.1.
BC028394 mRNA. Translation: AAH28394.2.
BC146803 mRNA. Translation: AAI46804.1. Frameshift.
CCDSCCDS9264.1. [Q8N697-1]
RefSeqNP_663623.1. NM_145648.3. [Q8N697-1]
UniGeneHs.507260.

3D structure databases

ProteinModelPortalQ8N697.
SMRQ8N697. Positions 32-557.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125714. 3 interactions.
IntActQ8N697. 1 interaction.
STRING9606.ENSP00000266771.

Chemistry

GuidetoPHARMACOLOGY987.

Protein family/group databases

TCDB2.A.17.3.11. the proton-dependent oligopeptide transporter (pot) family.

PTM databases

PhosphoSiteQ8N697.

Polymorphism databases

DMDM74751048.

Proteomic databases

MaxQBQ8N697.
PaxDbQ8N697.
PRIDEQ8N697.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000266771; ENSP00000266771; ENSG00000139370. [Q8N697-1]
ENST00000544112; ENSP00000439946; ENSG00000139370. [Q8N697-2]
GeneID121260.
KEGGhsa:121260.
UCSCuc001uhu.2. human. [Q8N697-1]

Organism-specific databases

CTD121260.
GeneCardsGC12M129277.
HGNCHGNC:23090. SLC15A4.
HPAHPA016713.
neXtProtNX_Q8N697.
PharmGKBPA134928948.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG3104.
HOGENOMHOG000143436.
HOVERGENHBG108431.
KOK14638.
OMAATGFLVM.
OrthoDBEOG7FNC8C.
PhylomeDBQ8N697.
TreeFamTF330897.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.
REACT_19419. Amino acid and oligopeptide SLC transporters.

Gene expression databases

ArrayExpressQ8N697.
BgeeQ8N697.
CleanExHS_SLC15A4.
GenevestigatorQ8N697.

Family and domain databases

InterProIPR016196. MFS_dom_general_subst_transpt.
IPR000109. POT_fam.
IPR018456. PTR2_symporter_CS.
[Graphical view]
PANTHERPTHR11654. PTHR11654. 1 hit.
PfamPF00854. PTR2. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 3 hits.
PROSITEPS01023. PTR2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC15A4. human.
GenomeRNAi121260.
NextBio80688.
PROQ8N697.

Entry information

Entry nameS15A4_HUMAN
AccessionPrimary (citable) accession number: Q8N697
Secondary accession number(s): A6H8Y9 expand/collapse secondary AC list , B3KTK1, Q71M34, Q7Z5F8, Q8TAH0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM