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Protein

Cleavage and polyadenylation specificity factor subunit 7

Gene

CPSF7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Binds to cleavage and polyadenylation RNA substrates.1 Publication

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000149532-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72187. mRNA 3'-end processing.
R-HSA-77595. Processing of Intronless Pre-mRNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Cleavage and polyadenylation specificity factor subunit 7
Alternative name(s):
Cleavage and polyadenylation specificity factor 59 kDa subunit
Short name:
CFIm59
Short name:
CPSF 59 kDa subunit
Pre-mRNA cleavage factor Im 59 kDa subunit
Gene namesi
Name:CPSF7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:30098. CPSF7.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: UniProtKB
  • mRNA cleavage factor complex Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi79869.
OpenTargetsiENSG00000149532.
PharmGKBiPA165543380.

Polymorphism and mutation databases

BioMutaiCPSF7.
DMDMi74759932.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000815271 – 471Cleavage and polyadenylation specificity factor subunit 7Add BLAST471

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei203PhosphothreonineCombined sources1
Modified residuei205PhosphoserineBy similarity1
Modified residuei413PhosphoserineCombined sources1
Modified residuei423PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8N684.
MaxQBiQ8N684.
PaxDbiQ8N684.
PeptideAtlasiQ8N684.
PRIDEiQ8N684.

PTM databases

iPTMnetiQ8N684.
PhosphoSitePlusiQ8N684.
SwissPalmiQ8N684.

Miscellaneous databases

PMAP-CutDBQ8N684.

Expressioni

Gene expression databases

BgeeiENSG00000149532.
ExpressionAtlasiQ8N684. baseline and differential.
GenevisibleiQ8N684. HS.

Organism-specific databases

HPAiHPA041094.

Interactioni

Subunit structurei

Component of the cleavage factor Im (CFIm) complex, composed of, at least, NUDT21/CPSF5 and CPSF6 or CPSF7. Within the cleavage factor Im complex, the NUDT21/CPSF5 homodimer is at the core of a heterotetramer, and is clasped by two additional subunits (CPSF6 or CPSF7). Interacts with NUDT21/CPSF5.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AESQ081173EBI-746909,EBI-717810
ARMC7Q9H6L43EBI-746909,EBI-742909
ATXN1P542532EBI-746909,EBI-930964
CLK2P497603EBI-746909,EBI-750020
EPM2AIP1Q7L7753EBI-746909,EBI-6255981
NCK2O436394EBI-746909,EBI-713635
NUDT21O438095EBI-746909,EBI-355720
RUNX1T1Q06455-43EBI-746909,EBI-10224192
SEC23BQ154373EBI-746909,EBI-742673
SMAD3P840223EBI-746909,EBI-347161
SNRNP25Q9BV903EBI-746909,EBI-9675976
U2AF2P263682EBI-746909,EBI-742339

Protein-protein interaction databases

BioGridi122957. 79 interactors.
IntActiQ8N684. 52 interactors.
MINTiMINT-1469392.
STRINGi9606.ENSP00000345412.

Structurei

Secondary structure

1471
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi83 – 87Combined sources5
Helixi95 – 104Combined sources10
Beta strandi110 – 117Combined sources8
Turni119 – 121Combined sources3
Beta strandi124 – 133Combined sources10
Helixi135 – 144Combined sources10
Beta strandi156 – 159Combined sources4
Helixi162 – 176Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N9UX-ray1.92C/I50-182[»]
ProteinModelPortaliQ8N684.
SMRiQ8N684.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8N684.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 162RRMPROSITE-ProRule annotationAdd BLAST81

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi51 – 54Poly-Pro4
Compositional biasi218 – 329Pro-richAdd BLAST112
Compositional biasi418 – 469Arg-richAdd BLAST52

Sequence similaritiesi

Belongs to the RRM CPSF6/7 family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4849. Eukaryota.
ENOG4111NBM. LUCA.
GeneTreeiENSGT00730000110905.
HOGENOMiHOG000111137.
HOVERGENiHBG056699.
InParanoidiQ8N684.
KOiK14398.
OMAiRRGPFEG.
OrthoDBiEOG091G0CVC.
PhylomeDBiQ8N684.
TreeFamiTF316430.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N684-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEGVDLIDI YADEEFNQDP EFNNTDQIDL YDDVLTATSQ PSDDRSSSTE
60 70 80 90 100
PPPPVRQEPS PKPNNKTPAI LYTYSGLRNR RAAVYVGSFS WWTTDQQLIQ
110 120 130 140 150
VIRSIGVYDV VELKFAENRA NGQSKGYAEV VVASENSVHK LLELLPGKVL
160 170 180 190 200
NGEKVDVRPA TRQNLSQFEA QARKRECVRV PRGGIPPRAH SRDSSDSADG
210 220 230 240 250
RATPSENLVP SSARVDKPPS VLPYFNRPPS ALPLMGLPPP PIPPPPPLSS
260 270 280 290 300
SFGVPPPPPG IHYQHLMPPP PRLPPHLAVP PPGAIPPALH LNPAFFPPPN
310 320 330 340 350
ATVGPPPDTY MKASAPYNHH GSRDSGPPPS TVSEAEFEDI MKRNRAISSS
360 370 380 390 400
AISKAVSGAS AGDYSDAIET LLTAIAVIKQ SRVANDERCR VLISSLKDCL
410 420 430 440 450
HGIEAKSYSV GASGSSSRKR HRSRERSPSR SRESSRRHRD LLHNEDRHDD
460 470
YFQERNREHE RHRDRERDRH H
Length:471
Mass (Da):52,050
Last modified:October 1, 2002 - v1
Checksum:i69529E441D742CF9
GO
Isoform 2 (identifier: Q8N684-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     176-184: Missing.

Show »
Length:462
Mass (Da):51,096
Checksum:iA3E41F7CB8340247
GO
Isoform 3 (identifier: Q8N684-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGRPESAGGGSRGPFEGGGRARRAGGIFLTLSILRTRDLPSGAM

Show »
Length:514
Mass (Da):56,375
Checksum:i37D5BE0605F7A0FB
GO

Sequence cautioni

The sequence AAH18135 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB14118 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAD97884 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti335A → V in CAD97884 (PubMed:17974005).Curated1
Sequence conflicti353S → F in CAD97884 (PubMed:17974005).Curated1
Sequence conflicti387E → D in BAB14118 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0389751M → MGRPESAGGGSRGPFEGGGR ARRAGGIFLTLSILRTRDLP SGAM in isoform 3. Curated1
Alternative sequenceiVSP_017194176 – 184Missing in isoform 2. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ275970 mRNA. Translation: CAC81661.1.
AK022591 mRNA. Translation: BAB14118.1. Different initiation.
AK096343 mRNA. Translation: BAG53266.1.
AL512759 mRNA. Translation: CAC21678.1.
BX537888 mRNA. Translation: CAD97884.1. Different initiation.
AP003108 Genomic DNA. No translation available.
BC018135 mRNA. Translation: AAH18135.1. Different initiation.
CCDSiCCDS44619.1. [Q8N684-1]
CCDS44620.1. [Q8N684-2]
CCDS8006.2. [Q8N684-3]
RefSeqiNP_001129512.1. NM_001136040.2. [Q8N684-1]
NP_001136037.1. NM_001142565.1. [Q8N684-2]
NP_079087.3. NM_024811.3. [Q8N684-3]
XP_005274356.1. XM_005274299.4. [Q8N684-1]
XP_011543560.1. XM_011545258.2. [Q8N684-1]
XP_011543561.1. XM_011545259.2. [Q8N684-2]
UniGeneiHs.718984.

Genome annotation databases

EnsembliENST00000340437; ENSP00000345412; ENSG00000149532. [Q8N684-3]
ENST00000394888; ENSP00000378352; ENSG00000149532. [Q8N684-1]
ENST00000439958; ENSP00000397203; ENSG00000149532. [Q8N684-2]
ENST00000448745; ENSP00000407394; ENSG00000149532. [Q8N684-2]
GeneIDi79869.
KEGGihsa:79869.
UCSCiuc001nrp.4. human. [Q8N684-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ275970 mRNA. Translation: CAC81661.1.
AK022591 mRNA. Translation: BAB14118.1. Different initiation.
AK096343 mRNA. Translation: BAG53266.1.
AL512759 mRNA. Translation: CAC21678.1.
BX537888 mRNA. Translation: CAD97884.1. Different initiation.
AP003108 Genomic DNA. No translation available.
BC018135 mRNA. Translation: AAH18135.1. Different initiation.
CCDSiCCDS44619.1. [Q8N684-1]
CCDS44620.1. [Q8N684-2]
CCDS8006.2. [Q8N684-3]
RefSeqiNP_001129512.1. NM_001136040.2. [Q8N684-1]
NP_001136037.1. NM_001142565.1. [Q8N684-2]
NP_079087.3. NM_024811.3. [Q8N684-3]
XP_005274356.1. XM_005274299.4. [Q8N684-1]
XP_011543560.1. XM_011545258.2. [Q8N684-1]
XP_011543561.1. XM_011545259.2. [Q8N684-2]
UniGeneiHs.718984.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N9UX-ray1.92C/I50-182[»]
ProteinModelPortaliQ8N684.
SMRiQ8N684.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122957. 79 interactors.
IntActiQ8N684. 52 interactors.
MINTiMINT-1469392.
STRINGi9606.ENSP00000345412.

PTM databases

iPTMnetiQ8N684.
PhosphoSitePlusiQ8N684.
SwissPalmiQ8N684.

Polymorphism and mutation databases

BioMutaiCPSF7.
DMDMi74759932.

Proteomic databases

EPDiQ8N684.
MaxQBiQ8N684.
PaxDbiQ8N684.
PeptideAtlasiQ8N684.
PRIDEiQ8N684.

Protocols and materials databases

DNASUi79869.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000340437; ENSP00000345412; ENSG00000149532. [Q8N684-3]
ENST00000394888; ENSP00000378352; ENSG00000149532. [Q8N684-1]
ENST00000439958; ENSP00000397203; ENSG00000149532. [Q8N684-2]
ENST00000448745; ENSP00000407394; ENSG00000149532. [Q8N684-2]
GeneIDi79869.
KEGGihsa:79869.
UCSCiuc001nrp.4. human. [Q8N684-1]

Organism-specific databases

CTDi79869.
DisGeNETi79869.
GeneCardsiCPSF7.
HGNCiHGNC:30098. CPSF7.
HPAiHPA041094.
neXtProtiNX_Q8N684.
OpenTargetsiENSG00000149532.
PharmGKBiPA165543380.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4849. Eukaryota.
ENOG4111NBM. LUCA.
GeneTreeiENSGT00730000110905.
HOGENOMiHOG000111137.
HOVERGENiHBG056699.
InParanoidiQ8N684.
KOiK14398.
OMAiRRGPFEG.
OrthoDBiEOG091G0CVC.
PhylomeDBiQ8N684.
TreeFamiTF316430.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000149532-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72187. mRNA 3'-end processing.
R-HSA-77595. Processing of Intronless Pre-mRNAs.

Miscellaneous databases

ChiTaRSiCPSF7. human.
EvolutionaryTraceiQ8N684.
GeneWikiiFLJ12529.
GenomeRNAii79869.
PMAP-CutDBQ8N684.
PROiQ8N684.

Gene expression databases

BgeeiENSG00000149532.
ExpressionAtlasiQ8N684. baseline and differential.
GenevisibleiQ8N684. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCPSF7_HUMAN
AccessioniPrimary (citable) accession number: Q8N684
Secondary accession number(s): B3KU04
, C9K0Q4, Q7Z3H9, Q9H025, Q9H9V1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.