Reviewed,
UniProtKB/Swiss-Prot Q8N684 (CPSF7_HUMAN)
Last modified
February 9, 2010.
Version 72.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Cleavage and polyadenylation specificity factor subunit 7 Alternative name(s): Cleavage and polyadenylation specificity factor 59 kDa subunit Short name=CPSF 59 kDa subunit Pre-mRNA cleavage factor Im 59 kDa subunit | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Complete proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 471 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates. Ref.5 |
| Subunit structure | Probable component of the cleavage factor Im (CFIm) complex, composed at least of NUDT21/CPSF5 and CPSF6 or CPSF7. |
| Subcellular location | Nucleus Probable. |
| Sequence similarities | Belongs to the RRM CPSF6/7 family. Contains 1 RRM (RNA recognition motif) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | mRNA processing |
| Cellular component | Nucleus |
| Coding sequence diversity | Alternative splicing |
| Ligand | RNA-binding |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | RNA splicing Inferred from Experiment. Source: Reactome mRNA processingInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide bindingInferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q8N684-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q8N684-2) The sequence of this isoform differs from the canonical sequence as follows: 176-184: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 471 | 471 | Cleavage and polyadenylation specificity factor subunit 7 | PRO_0000081527 | |||||
Regions | |||||||||
| Domain | 82 – 162 | 81 | RRM | ||||||
| Compositional bias | 51 – 54 | 4 | Poly-Pro | ||||||
| Compositional bias | 218 – 329 | 112 | Pro-rich | ||||||
| Compositional bias | 418 – 469 | 52 | Arg-rich | ||||||
Amino acid modifications | |||||||||
| Modified residue | 48 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 72 | 1 | Phosphotyrosine Ref.6 | ||||||
| Modified residue | 73 | 1 | Phosphothreonine Ref.6 | ||||||
| Modified residue | 197 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 203 | 1 | Phosphothreonine Ref.12 Ref.10 | ||||||
| Modified residue | 413 | 1 | Phosphoserine Ref.10 Ref.8 | ||||||
| Modified residue | 423 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 429 | 1 | Phosphoserine Ref.7 | ||||||
Natural variations | |||||||||
| Alternative sequence | 176 – 184 | 9 | Missing in isoform 2. | VSP_017194 | |||||
Experimental info | |||||||||
| Sequence conflict | 335 | 1 | A → V in CAD97884. Ref.2 | ||||||
| Sequence conflict | 353 | 1 | S → F in CAD97884. Ref.2 | ||||||
| Sequence conflict | 387 | 1 | E → D Ref.4 | ||||||
Sequences
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References
| [1] | "Cloning of a second SR protein related subunit of human pre-mRNA cleavage factor I." Rueegsegger U., Blank D., Dettwiler S., Keller W. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Cervix carcinoma. |
| [2] | "The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 338-471 (ISOFORMS 1/2). Tissue: Bone marrow and Embryonic brain. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Uterus. |
| [4] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 201-471 (ISOFORMS 1/2). Tissue: Teratocarcinoma. |
| [5] | "Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors." Rueegsegger U., Beyer K., Keller W. J. Biol. Chem. 271:6107-6113(1996) [PubMed: 8626397] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A CLEAVAGE FACTOR IM COMPLEX, RNA-BINDING. |
| [6] | "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed: 15144186] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-72 AND THR-73, MASS SPECTROMETRY. Tissue: T-cell. |
| [7] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423 AND SER-429, MASS SPECTROMETRY. Tissue: Epithelium. |
| [8] | "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-413, MASS SPECTROMETRY. Tissue: Epithelium. |
| [9] | "Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-197, MASS SPECTROMETRY. |
| [10] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-203 AND SER-413, MASS SPECTROMETRY. |
| [11] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [12] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48 AND THR-203, MASS SPECTROMETRY. Tissue: T-cell. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AJ275970 mRNA. Translation: CAC81661.1. BX537888 mRNA. Translation: CAD97884.1. Different initiation. AL512759 mRNA. Translation: CAC21678.1. BC018135 mRNA. Translation: AAH18135.1. AK022591 mRNA. Translation: BAB14118.1. Different initiation. AK096343 mRNA. Translation: BAG53266.1. |
| IPI | IPI00550821. IPI00719106. |
| RefSeq | NP_001129512.1. NP_001136037.1. NP_079087.3. |
| UniGene | Hs.444552 Hs.718984 |
3D structure databases | |
| SMR | Q8N684. Positions 80-164. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q8N684. 8 interactions. |
| STRING | Q8N684. |
PTM databases | |
| PhosphoSite | Q8N684. |
Proteomic databases | |
| PRIDE | Q8N684. |
Genome annotation databases | |
| Ensembl | ENST00000340437; ENSP00000345412; ENSG00000149532; Homo sapiens. [Genome view] ENST00000394888; ENSP00000378352; ENSG00000149532; Homo sapiens. [Genome view] |
| GeneID | 79869. |
| KEGG | hsa:79869. |
| UCSC | uc001nro.1. human. uc001nrp.1. human. |
Organism-specific databases | |
| CTD | 79869. |
| GeneCards | GC11M060926. |
| H-InvDB | HIX0009690. |
| HGNC | HGNC:30098. CPSF7. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG09513. |
| HOGENOM | HBG505348. |
| HOVERGEN | Q8N684. |
| InParanoid | Q8N684. |
| OrthoDB | EOG92RGSS. |
| PhylomeDB | Q8N684. |
Enzyme and pathway databases | |
| Reactome | REACT_125. Processing of Capped Intron-Containing Pre-mRNA. REACT_1788. Transcription. REACT_71. Gene Expression. |
Gene expression databases | |
| ArrayExpress | Q8N684. |
| Bgee | Q8N684. |
| Genevestigator | Q8N684. |
| GermOnline | ENSG00000149532. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR012677. a_b_plait_nuc_bd. IPR000504. RRM_RNP1. [Graphical view] |
| Gene3D | G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit. |
| Pfam | PF00076. RRM_1. 1 hit. [Graphical view] |
| SMART | SM00360. RRM. 1 hit. [Graphical view] |
| PROSITE | PS50102. RRM. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 69628. |
| PMAP-CutDB | Q8N684. |
Entry information
| Entry name | CPSF7_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q8N684 Secondary accession number(s): B3KU04 Q9H9V1 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 11 Human chromosome 11: entries, gene names and cross-references to MIM |
| SIMILARITY comments Index of protein domains and families |

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