ID   DRAM1_HUMAN             Reviewed;         238 AA.
AC   Q8N682; Q7L3E3; Q9NUN1;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   19-JAN-2010, entry version 50.
DE   RecName: Full=DNA damage-regulated autophagy modulator protein 1;
DE   AltName: Full=Damage-regulated autophagy modulator;
GN   Name=DRAM1; Synonyms=DRAM;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Liver, and Skin;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 78-238.
RC   TISSUE=Placenta;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX   PubMed=16839881; DOI=10.1016/j.cell.2006.05.034;
RA   Crighton D., Wilkinson S., O'Prey J., Syed N., Smith P.,
RA   Harrison P.R., Gasco M., Garrone O., Crook T., Ryan K.M.;
RT   "DRAM, a p53-induced modulator of autophagy, is critical for
RT   apoptosis.";
RL   Cell 126:121-134(2006).
RN   [4]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=17304243; DOI=10.1038/sj.cdd.4402108;
RA   Crighton D., O'prey J., Bell H.S., Ryan K.M.;
RT   "p73 regulates DRAM-independent autophagy that does not contribute to
RT   programmed cell death.";
RL   Cell Death Differ. 14:1071-1079(2007).
CC   -!- FUNCTION: Lysosomal modulator of autophagy that plays a central
CC       role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-
CC       mediated autophagy.
CC   -!- SUBCELLULAR LOCATION: Lysosome membrane; Multi-pass membrane
CC       protein.
CC   -!- INDUCTION: By p53/TP53 and p73/TP73. Directly activated by
CC       p53/TP53. Significantly down-regulated in tumor cell lines by
CC       methylation-dependent transcriptional silencing.
CC   -!- SIMILARITY: Belongs to the DRAM/TMEM150 family.
CC   -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology
CC       and Haematology;
CC       URL="http://atlasgeneticsoncology.org/Genes/DRAMID44093ch12q23.html";
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DR   EMBL; BC013773; AAH13773.4; -; mRNA.
DR   EMBL; BC018435; AAH18435.1; -; mRNA.
DR   EMBL; AK002121; BAA92091.1; ALT_INIT; mRNA.
DR   IPI; IPI00300813; -.
DR   RefSeq; NP_060840.2; -.
DR   UniGene; Hs.525634; -.
DR   STRING; Q8N682; -.
DR   Ensembl; ENST00000258534; ENSP00000258534; ENSG00000136048; Homo sapiens.
DR   GeneID; 55332; -.
DR   KEGG; hsa:55332; -.
DR   UCSC; uc001tix.1; human.
DR   CTD; 55332; -.
DR   GeneCards; GC12P100795; -.
DR   HGNC; HGNC:25645; DRAM1.
DR   MIM; 610776; gene.
DR   eggNOG; prNOG11973; -.
DR   HOVERGEN; Q8N682; -.
DR   InParanoid; Q8N682; -.
DR   OMA; FLTFIHD; -.
DR   OrthoDB; EOG9K6JPM; -.
DR   PhylomeDB; Q8N682; -.
DR   NextBio; 59626; -.
DR   ArrayExpress; Q8N682; -.
DR   Bgee; Q8N682; -.
DR   Genevestigator; Q8N682; -.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005764; C:lysosome; IEA:UniProtKB-KW.
DR   GO; GO:0006915; P:apoptosis; IEA:UniProtKB-KW.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   InterPro; IPR019402; Frag1/DRAM/Sfk1.
DR   Pfam; PF10277; Frag1; 1.
PE   2: Evidence at transcript level;
KW   Apoptosis; Autophagy; Complete proteome; Lysosome; Membrane;
KW   Transmembrane.
FT   CHAIN         1    238       DNA damage-regulated autophagy modulator
FT                                protein 1.
FT                                /FTId=PRO_0000287436.
FT   TRANSMEM      9     29       Potential.
FT   TRANSMEM     53     73       Potential.
FT   TRANSMEM     91    111       Potential.
FT   TRANSMEM    116    136       Potential.
FT   TRANSMEM    161    181       Potential.
FT   TRANSMEM    200    220       Potential.
SQ   SEQUENCE   238 AA;  26253 MW;  511875677737F6C0 CRC64;
     MLCFLRGMAF VPFLLVTWSS AAFIISYVVA VLSGHVNPFL PYISDTGTTP PESGIFGFMI
     NFSAFLGAAT MYTRYKIVQK QNQTCYFSTP VFNLVSLVLG LVGCFGMGIV ANFQELAVPV
     VHDGGALLAF VCGVVYTLLQ SIISYKSCPQ WNSLSTCHIR MVISAVSCAA VIPMIVCASL
     ISITKLEWNP REKDYVYHVV SAICEWTVAF GFIFYFLTFI QDFQSVTLRI STEINGDI
//