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Protein

DNA damage-regulated autophagy modulator protein 1

Gene

DRAM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy.2 Publications

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • autophagy Source: UniProtKB-KW
  • regulation of autophagy Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Autophagy

Names & Taxonomyi

Protein namesi
Recommended name:
DNA damage-regulated autophagy modulator protein 1
Alternative name(s):
Damage-regulated autophagy modulator
Gene namesi
Name:DRAM1
Synonyms:DRAM
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:25645. DRAM1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2921HelicalSequence analysisAdd
BLAST
Transmembranei53 – 7321HelicalSequence analysisAdd
BLAST
Transmembranei91 – 11121HelicalSequence analysisAdd
BLAST
Transmembranei116 – 13621HelicalSequence analysisAdd
BLAST
Transmembranei161 – 18121HelicalSequence analysisAdd
BLAST
Transmembranei200 – 22021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • lysosomal membrane Source: UniProtKB-SubCell
  • lysosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165512564.

Polymorphism and mutation databases

BioMutaiDRAM1.
DMDMi74729109.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238DNA damage-regulated autophagy modulator protein 1PRO_0000287436Add
BLAST

Proteomic databases

PaxDbiQ8N682.
PRIDEiQ8N682.

PTM databases

PhosphoSiteiQ8N682.

Expressioni

Inductioni

By p53/TP53 and p73/TP73. Directly activated by p53/TP53. Significantly down-regulated in tumor cell lines by methylation-dependent transcriptional silencing.2 Publications

Gene expression databases

BgeeiQ8N682.
ExpressionAtlasiQ8N682. baseline and differential.
GenevisibleiQ8N682. HS.

Organism-specific databases

HPAiCAB034160.
HPA044812.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
C1orf21Q9H2463EBI-10305400,EBI-10305393

Protein-protein interaction databases

BioGridi120613. 3 interactions.
IntActiQ8N682. 1 interaction.
STRINGi9606.ENSP00000258534.

Structurei

3D structure databases

ProteinModelPortaliQ8N682.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DRAM/TMEM150 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4320. Eukaryota.
ENOG4111KNB. LUCA.
GeneTreeiENSGT00730000110891.
HOGENOMiHOG000047053.
InParanoidiQ8N682.
OMAiFLTFIHD.
OrthoDBiEOG7VDXPT.
PhylomeDBiQ8N682.
TreeFamiTF314508.

Family and domain databases

InterProiIPR019402. Frag1/DRAM/Sfk1.
[Graphical view]
PfamiPF10277. Frag1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N682-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLCFLRGMAF VPFLLVTWSS AAFIISYVVA VLSGHVNPFL PYISDTGTTP
60 70 80 90 100
PESGIFGFMI NFSAFLGAAT MYTRYKIVQK QNQTCYFSTP VFNLVSLVLG
110 120 130 140 150
LVGCFGMGIV ANFQELAVPV VHDGGALLAF VCGVVYTLLQ SIISYKSCPQ
160 170 180 190 200
WNSLSTCHIR MVISAVSCAA VIPMIVCASL ISITKLEWNP REKDYVYHVV
210 220 230
SAICEWTVAF GFIFYFLTFI QDFQSVTLRI STEINGDI
Length:238
Mass (Da):26,253
Last modified:October 1, 2002 - v1
Checksum:i511875677737F6C0
GO
Isoform 2 (identifier: Q8N682-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-222: Missing.

Note: No experimental confirmation available.
Show »
Length:128
Mass (Da):13,972
Checksum:i16DAFB2B333BEAB7
GO

Sequence cautioni

The sequence BAA92091.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei113 – 222110Missing in isoform 2. 1 PublicationVSP_056980Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002121 mRNA. Translation: BAA92091.1. Different initiation.
AK297785 mRNA. Translation: BAH12666.1.
AC063950 Genomic DNA. No translation available.
AC079907 Genomic DNA. No translation available.
AC084398 Genomic DNA. No translation available.
BC013773 mRNA. Translation: AAH13773.4.
BC018435 mRNA. Translation: AAH18435.1.
CCDSiCCDS41823.1. [Q8N682-1]
RefSeqiNP_060840.2. NM_018370.2. [Q8N682-1]
UniGeneiHs.525634.

Genome annotation databases

EnsembliENST00000258534; ENSP00000258534; ENSG00000136048. [Q8N682-1]
ENST00000544152; ENSP00000445827; ENSG00000136048. [Q8N682-2]
GeneIDi55332.
KEGGihsa:55332.
UCSCiuc001tix.4. human. [Q8N682-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002121 mRNA. Translation: BAA92091.1. Different initiation.
AK297785 mRNA. Translation: BAH12666.1.
AC063950 Genomic DNA. No translation available.
AC079907 Genomic DNA. No translation available.
AC084398 Genomic DNA. No translation available.
BC013773 mRNA. Translation: AAH13773.4.
BC018435 mRNA. Translation: AAH18435.1.
CCDSiCCDS41823.1. [Q8N682-1]
RefSeqiNP_060840.2. NM_018370.2. [Q8N682-1]
UniGeneiHs.525634.

3D structure databases

ProteinModelPortaliQ8N682.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120613. 3 interactions.
IntActiQ8N682. 1 interaction.
STRINGi9606.ENSP00000258534.

PTM databases

PhosphoSiteiQ8N682.

Polymorphism and mutation databases

BioMutaiDRAM1.
DMDMi74729109.

Proteomic databases

PaxDbiQ8N682.
PRIDEiQ8N682.

Protocols and materials databases

DNASUi55332.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000258534; ENSP00000258534; ENSG00000136048. [Q8N682-1]
ENST00000544152; ENSP00000445827; ENSG00000136048. [Q8N682-2]
GeneIDi55332.
KEGGihsa:55332.
UCSCiuc001tix.4. human. [Q8N682-1]

Organism-specific databases

CTDi55332.
GeneCardsiDRAM1.
HGNCiHGNC:25645. DRAM1.
HPAiCAB034160.
HPA044812.
MIMi610776. gene.
neXtProtiNX_Q8N682.
PharmGKBiPA165512564.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4320. Eukaryota.
ENOG4111KNB. LUCA.
GeneTreeiENSGT00730000110891.
HOGENOMiHOG000047053.
InParanoidiQ8N682.
OMAiFLTFIHD.
OrthoDBiEOG7VDXPT.
PhylomeDBiQ8N682.
TreeFamiTF314508.

Miscellaneous databases

ChiTaRSiDRAM1. human.
GenomeRNAii55332.
NextBioi35479553.
PROiQ8N682.
SOURCEiSearch...

Gene expression databases

BgeeiQ8N682.
ExpressionAtlasiQ8N682. baseline and differential.
GenevisibleiQ8N682. HS.

Family and domain databases

InterProiIPR019402. Frag1/DRAM/Sfk1.
[Graphical view]
PfamiPF10277. Frag1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 78-238 (ISOFORM 1).
    Tissue: Placenta.
  2. "The finished DNA sequence of human chromosome 12."
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Liver and Skin.
  4. "DRAM, a p53-induced modulator of autophagy, is critical for apoptosis."
    Crighton D., Wilkinson S., O'Prey J., Syed N., Smith P., Harrison P.R., Gasco M., Garrone O., Crook T., Ryan K.M.
    Cell 126:121-134(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
  5. "p73 regulates DRAM-independent autophagy that does not contribute to programmed cell death."
    Crighton D., O'prey J., Bell H.S., Ryan K.M.
    Cell Death Differ. 14:1071-1079(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiDRAM1_HUMAN
AccessioniPrimary (citable) accession number: Q8N682
Secondary accession number(s): B7Z4T0, Q7L3E3, Q9NUN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: October 1, 2002
Last modified: April 13, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.