Reviewed, UniProtKB/Swiss-Prot Q8N682 (DRAM_HUMAN)

Names and origin

Protein names

Recommended name:
    Damage-regulated autophagy modulator

Gene names

Name:

DRAM

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy. Ref.3 Ref.4
Subcellular location	Lysosome membrane; Multi-pass membrane protein. Ref.3
Induction	By p53/TP53 and p73/TP73. Directly activated by p53/TP53. Significantly down-regulated in tumor cell lines by methylation-dependent transcriptional silencing. Ref.3 Ref.4
Sequence similarities	Belongs to the TMEM77/TMEM150 family.

Ontologies

Keywords
Biological process	Apoptosis Autophagy
Cellular component	Lysosome Membrane
Domain	Transmembrane
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW autophagy Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Damage-regulated autophagy modulator		PRO_0000287436
Regions
	Transmembrane	9 – 29	21	Potential
	Transmembrane	53 – 73	21	Potential
	Transmembrane	91 – 111	21	Potential
	Transmembrane	116 – 136	21	Potential
	Transmembrane	161 – 181	21	Potential
	Transmembrane	200 – 220	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8N682-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 511875677737F6C0	FASTA	238	26,253
	10 20 30 40 50 60 MLCFLRGMAF VPFLLVTWSS AAFIISYVVA VLSGHVNPFL PYISDTGTTP PESGIFGFMI 70 80 90 100 110 120 NFSAFLGAAT MYTRYKIVQK QNQTCYFSTP VFNLVSLVLG LVGCFGMGIV ANFQELAVPV 130 140 150 160 170 180 VHDGGALLAF VCGVVYTLLQ SIISYKSCPQ WNSLSTCHIR MVISAVSCAA VIPMIVCASL 190 200 210 220 230 ISITKLEWNP REKDYVYHVV SAICEWTVAF GFIFYFLTFI QDFQSVTLRI STEINGDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver and Skin.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 78-238. Tissue: Placenta.
[3]	"DRAM, a p53-induced modulator of autophagy, is critical for apoptosis." Crighton D., Wilkinson S., O'Prey J., Syed N., Smith P., Harrison P.R., Gasco M., Garrone O., Crook T., Ryan K.M. Cell 126:121-134(2006) [PubMed: 16839881] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
[4]	"p73 regulates DRAM-independent autophagy that does not contribute to programmed cell death." Crighton D., O'prey J., Bell H.S., Ryan K.M. Cell Death Differ. 14:1071-1079(2007) [PubMed: 17304243] [Abstract] Cited for: FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC013773 mRNA. Translation: AAH13773.4. BC018435 mRNA. Translation: AAH18435.1. AK002121 mRNA. Translation: BAA92091.1. Different initiation.
IPI	IPI00300813.
RefSeq	NP_060840.2.
UniGene	Hs.525634
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000136048. Homo sapiens. [Contig view]
GeneID	55332.
KEGG	hsa:55332.
Organism-specific databases
GeneCards	GC12P100795.
MIM	610776. gene.
Phylogenomic databases
HOGENOM	Q8N682.
HOVERGEN	Q8N682.
OMA	Q8N682. FLTFIHD.
Gene expression databases
ArrayExpress	Q8N682.
Bgee	Q8N682.
Family and domain databases
InterPro	IPR019402. Frag1/DRAM/Sfk1. [Graphical view]
Pfam	PF10277. Frag1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	59626.
SOURCE	Search...

Entry information

Entry name

DRAM_HUMAN

Accession

Primary (citable) accession number: Q8N682
Secondary accession number(s): Q7L3E3, Q9NUN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)