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Protein

Lysoplasmalogenase

Gene

TMEM86B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme catalyzing the degradation of lysoplasmalogen. Lysoplasmalogens are formed by the hydrolysis of the abundant membrane glycerophospholipids plasmalogens. May control the respective levels of plasmalogens and lysoplasmalogens in cells and modulate cell membrane properties.1 Publication

Catalytic activityi

1-(1-alkenyl)-sn-glycero-3-phosphocholine + H2O = an aldehyde + sn-glycero-3-phosphocholine.1 Publication
1-(1-alkenyl)-sn-glycero-3-phosphoethanolamine + H2O = an aldehyde + sn-glycero-3-phosphoethanolamine.1 Publication

Enzyme regulationi

Competitively inhibited by lysophosphatidic acid.1 Publication

Kineticsi

  1. KM=45 µM for lysoplasmenyl-choline1 Publication
  2. KM=53 µM for lysoplasmenyl-ethanolamine1 Publication

pH dependencei

Optimum pH is 6.95 and 7.05 with lysoplasmenyl-ethanolamine and lysoplasmenyl-choline as substrates, respectively.1 Publication

GO - Molecular functioni

  1. alkenylglycerophosphocholine hydrolase activity Source: UniProtKB
  2. alkenylglycerophosphoethanolamine hydrolase activity Source: UniProtKB

GO - Biological processi

  1. ether lipid metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Lysoplasmalogenase (EC:3.3.2.2, EC:3.3.2.5)
Alternative name(s):
Transmembrane protein 86B
Gene namesi
Name:TMEM86B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:28448. TMEM86B.

Subcellular locationi

Membrane Curated; Multi-pass membrane protein Curated. Cytoplasm 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei21 – 4121HelicalSequence AnalysisAdd
BLAST
Transmembranei47 – 6721HelicalSequence AnalysisAdd
BLAST
Transmembranei85 – 10521HelicalSequence AnalysisAdd
BLAST
Transmembranei109 – 12921HelicalSequence AnalysisAdd
BLAST
Transmembranei147 – 16721HelicalSequence AnalysisAdd
BLAST
Transmembranei172 – 19221HelicalSequence AnalysisAdd
BLAST
Transmembranei198 – 21821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142670738.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226LysoplasmalogenasePRO_0000201841Add
BLAST

Proteomic databases

PRIDEiQ8N661.

Expressioni

Gene expression databases

BgeeiQ8N661.
CleanExiHS_TMEM86B.
GenevestigatoriQ8N661.

Organism-specific databases

HPAiHPA057689.

Interactioni

Subunit structurei

Homodimer.Curated

Protein-protein interaction databases

BioGridi129071. 2 interactions.
IntActiQ8N661. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ8N661.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TMEM86 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG74425.
GeneTreeiENSGT00390000007101.
HOGENOMiHOG000204433.
HOVERGENiHBG055092.
InParanoidiQ8N661.
KOiK18575.
OMAiSHLMILA.
OrthoDBiEOG7J9VQD.
PhylomeDBiQ8N661.
TreeFamiTF324663.

Family and domain databases

InterProiIPR012506. YhhN.
[Graphical view]
PfamiPF07947. YhhN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8N661-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDAGKAGQTL KTHCSAQRPD VCRWLSPFIL SCCVYFCLWI PEDQLSWFAA
60 70 80 90 100
LVKCLPVLCL AGFLWVMSPS GGYTQLLQGA LVCSAVGDAC LIWPAAFVPG
110 120 130 140 150
MAAFATAHLL YVWAFGFSPL QPGLLLLIIL APGPYLSLVL QHLEPDMVLP
160 170 180 190 200
VAAYGLILMA MLWRGLAQGG SAGWGALLFT LSDGVLAWDT FAQPLPHAHL
210 220
VIMTTYYAAQ LLITLSALRS PVPKTD
Length:226
Mass (Da):24,352
Last modified:May 18, 2010 - v2
Checksum:iBD5344268F221275
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti176 – 1761A → T.
Corresponds to variant rs35608872 [ dbSNP | Ensembl ].
VAR_052343
Natural varianti199 – 1991H → R.1 Publication
Corresponds to variant rs4644955 [ dbSNP | Ensembl ].
VAR_052344

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010327 Genomic DNA. No translation available.
BC023000 mRNA. Translation: AAH23000.1.
CCDSiCCDS12920.1.
RefSeqiNP_776165.3. NM_173804.4.
UniGeneiHs.135215.

Genome annotation databases

EnsembliENST00000327042; ENSP00000321038; ENSG00000180089.
GeneIDi255043.
KEGGihsa:255043.
UCSCiuc002qjt.3. human.

Polymorphism databases

DMDMi296452936.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010327 Genomic DNA. No translation available.
BC023000 mRNA. Translation: AAH23000.1.
CCDSiCCDS12920.1.
RefSeqiNP_776165.3. NM_173804.4.
UniGeneiHs.135215.

3D structure databases

ProteinModelPortaliQ8N661.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi129071. 2 interactions.
IntActiQ8N661. 1 interaction.

Polymorphism databases

DMDMi296452936.

Proteomic databases

PRIDEiQ8N661.

Protocols and materials databases

DNASUi255043.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000327042; ENSP00000321038; ENSG00000180089.
GeneIDi255043.
KEGGihsa:255043.
UCSCiuc002qjt.3. human.

Organism-specific databases

CTDi255043.
GeneCardsiGC19M055738.
H-InvDBHIX0174302.
HIX0202856.
HGNCiHGNC:28448. TMEM86B.
HPAiHPA057689.
neXtProtiNX_Q8N661.
PharmGKBiPA142670738.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG74425.
GeneTreeiENSGT00390000007101.
HOGENOMiHOG000204433.
HOVERGENiHBG055092.
InParanoidiQ8N661.
KOiK18575.
OMAiSHLMILA.
OrthoDBiEOG7J9VQD.
PhylomeDBiQ8N661.
TreeFamiTF324663.

Miscellaneous databases

GenomeRNAii255043.
NextBioi92454.
PROiQ8N661.

Gene expression databases

BgeeiQ8N661.
CleanExiHS_TMEM86B.
GenevestigatoriQ8N661.

Family and domain databases

InterProiIPR012506. YhhN.
[Graphical view]
PfamiPF07947. YhhN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-199.
    Tissue: Placenta.
  3. "Purification, identification, and cloning of lysoplasmalogenase, the enzyme that catalyzes hydrolysis of the vinyl ether bond of lysoplasmalogen."
    Wu L.C., Pfeiffer D.R., Calhoon E.A., Madiai F., Marcucci G., Liu S., Jurkowitz M.S.
    J. Biol. Chem. 286:24916-24930(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, DIMERIZATION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTM86B_HUMAN
AccessioniPrimary (citable) accession number: Q8N661
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 18, 2010
Last modified: February 4, 2015
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.