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Protein

Lysoplasmalogenase

Gene

TMEM86B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme catalyzing the degradation of lysoplasmalogen. Lysoplasmalogens are formed by the hydrolysis of the abundant membrane glycerophospholipids plasmalogens. May control the respective levels of plasmalogens and lysoplasmalogens in cells and modulate cell membrane properties.1 Publication

Catalytic activityi

1-(1-alkenyl)-sn-glycero-3-phosphocholine + H2O = an aldehyde + sn-glycero-3-phosphocholine.1 Publication
1-(1-alkenyl)-sn-glycero-3-phosphoethanolamine + H2O = an aldehyde + sn-glycero-3-phosphoethanolamine.1 Publication

Enzyme regulationi

Competitively inhibited by lysophosphatidic acid.1 Publication

Kineticsi

  1. KM=45 µM for lysoplasmenyl-choline1 Publication
  2. KM=53 µM for lysoplasmenyl-ethanolamine1 Publication

    pH dependencei

    Optimum pH is 6.95 and 7.05 with lysoplasmenyl-ethanolamine and lysoplasmenyl-choline as substrates, respectively.1 Publication

    GO - Molecular functioni

    • alkenylglycerophosphocholine hydrolase activity Source: UniProtKB
    • alkenylglycerophosphoethanolamine hydrolase activity Source: UniProtKB

    GO - Biological processi

    • ether lipid metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysoplasmalogenase (EC:3.3.2.2, EC:3.3.2.5)
    Alternative name(s):
    Transmembrane protein 86B
    Gene namesi
    Name:TMEM86B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640 Componenti: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:28448. TMEM86B.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei21 – 4121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei47 – 6721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei85 – 10521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei109 – 12921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei147 – 16721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei172 – 19221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei198 – 21821HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    • cytoplasm Source: UniProtKB
    • integral component of membrane Source: UniProtKB-KW
    • membrane Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142670738.

    Polymorphism and mutation databases

    BioMutaiTMEM86B.
    DMDMi296452936.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 226226LysoplasmalogenasePRO_0000201841Add
    BLAST

    Proteomic databases

    PRIDEiQ8N661.

    Expressioni

    Gene expression databases

    BgeeiQ8N661.
    CleanExiHS_TMEM86B.
    GenevisibleiQ8N661. HS.

    Organism-specific databases

    HPAiHPA057689.

    Interactioni

    Subunit structurei

    Homodimer.Curated

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BCL2L13Q9BXK53EBI-2548832,EBI-747430

    Protein-protein interaction databases

    BioGridi129071. 1 interaction.
    IntActiQ8N661. 2 interactions.
    STRINGi9606.ENSP00000321038.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8N661.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TMEM86 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG74425.
    GeneTreeiENSGT00390000007101.
    HOGENOMiHOG000204433.
    HOVERGENiHBG055092.
    InParanoidiQ8N661.
    KOiK18575.
    OMAiGQWAIAS.
    OrthoDBiEOG7J9VQD.
    PhylomeDBiQ8N661.
    TreeFamiTF324663.

    Family and domain databases

    InterProiIPR012506. YhhN.
    [Graphical view]
    PfamiPF07947. YhhN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8N661-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDAGKAGQTL KTHCSAQRPD VCRWLSPFIL SCCVYFCLWI PEDQLSWFAA
    60 70 80 90 100
    LVKCLPVLCL AGFLWVMSPS GGYTQLLQGA LVCSAVGDAC LIWPAAFVPG
    110 120 130 140 150
    MAAFATAHLL YVWAFGFSPL QPGLLLLIIL APGPYLSLVL QHLEPDMVLP
    160 170 180 190 200
    VAAYGLILMA MLWRGLAQGG SAGWGALLFT LSDGVLAWDT FAQPLPHAHL
    210 220
    VIMTTYYAAQ LLITLSALRS PVPKTD
    Length:226
    Mass (Da):24,352
    Last modified:May 18, 2010 - v2
    Checksum:iBD5344268F221275
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti176 – 1761A → T.
    Corresponds to variant rs35608872 [ dbSNP | Ensembl ].
    VAR_052343
    Natural varianti199 – 1991H → R.1 Publication
    Corresponds to variant rs4644955 [ dbSNP | Ensembl ].
    VAR_052344

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC010327 Genomic DNA. No translation available.
    BC023000 mRNA. Translation: AAH23000.1.
    CCDSiCCDS12920.1.
    RefSeqiNP_776165.3. NM_173804.4.
    UniGeneiHs.135215.

    Genome annotation databases

    EnsembliENST00000327042; ENSP00000321038; ENSG00000180089.
    GeneIDi255043.
    KEGGihsa:255043.
    UCSCiuc002qjt.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC010327 Genomic DNA. No translation available.
    BC023000 mRNA. Translation: AAH23000.1.
    CCDSiCCDS12920.1.
    RefSeqiNP_776165.3. NM_173804.4.
    UniGeneiHs.135215.

    3D structure databases

    ProteinModelPortaliQ8N661.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi129071. 1 interaction.
    IntActiQ8N661. 2 interactions.
    STRINGi9606.ENSP00000321038.

    Polymorphism and mutation databases

    BioMutaiTMEM86B.
    DMDMi296452936.

    Proteomic databases

    PRIDEiQ8N661.

    Protocols and materials databases

    DNASUi255043.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000327042; ENSP00000321038; ENSG00000180089.
    GeneIDi255043.
    KEGGihsa:255043.
    UCSCiuc002qjt.3. human.

    Organism-specific databases

    CTDi255043.
    GeneCardsiGC19M055738.
    H-InvDBHIX0174302.
    HIX0202856.
    HGNCiHGNC:28448. TMEM86B.
    HPAiHPA057689.
    neXtProtiNX_Q8N661.
    PharmGKBiPA142670738.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiNOG74425.
    GeneTreeiENSGT00390000007101.
    HOGENOMiHOG000204433.
    HOVERGENiHBG055092.
    InParanoidiQ8N661.
    KOiK18575.
    OMAiGQWAIAS.
    OrthoDBiEOG7J9VQD.
    PhylomeDBiQ8N661.
    TreeFamiTF324663.

    Miscellaneous databases

    GenomeRNAii255043.
    NextBioi92454.
    PROiQ8N661.

    Gene expression databases

    BgeeiQ8N661.
    CleanExiHS_TMEM86B.
    GenevisibleiQ8N661. HS.

    Family and domain databases

    InterProiIPR012506. YhhN.
    [Graphical view]
    PfamiPF07947. YhhN. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-199.
      Tissue: Placenta.
    3. "Purification, identification, and cloning of lysoplasmalogenase, the enzyme that catalyzes hydrolysis of the vinyl ether bond of lysoplasmalogen."
      Wu L.C., Pfeiffer D.R., Calhoon E.A., Madiai F., Marcucci G., Liu S., Jurkowitz M.S.
      J. Biol. Chem. 286:24916-24930(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, DIMERIZATION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiTM86B_HUMAN
    AccessioniPrimary (citable) accession number: Q8N661
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: May 18, 2010
    Last modified: July 22, 2015
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.