Q8N661 (TM86B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysoplasmalogenase
EC=3.3.2.2
EC=3.3.2.5

Alternative name(s):
Transmembrane protein 86B

Gene names

Name:

TMEM86B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Enzyme catalyzing the degradation of lysoplasmalogen. Lysoplasmalogens are formed by the hydrolysis of the abundant membrane glycerophospholipids plasmalogens. May control the respective levels of plasmalogens and lysoplasmalogens in cells and modulate cell membrane properties. Ref.3
Catalytic activity	1-(1-alkenyl)-sn-glycero-3-phosphocholine + H2O = an aldehyde + sn-glycero-3-phosphocholine. Ref.3 1-(1-alkenyl)-sn-glycero-3-phosphoethanolamine + H2O = an aldehyde + sn-glycero-3-phosphoethanolamine. Ref.3
Enzyme regulation	Competitively inhibited by lysophosphatidic acid. Ref.3
Subunit structure	Homodimer Probable. Ref.3
Subcellular location	Membrane; Multi-pass membrane protein Potential. Cytoplasm Ref.3.
Sequence similarities	Belongs to the TMEM86 family.
Biophysicochemical properties	Kinetic parameters: KM=45 µM for lysoplasmenyl-choline Ref.3 KM=53 µM for lysoplasmenyl-ethanolamine pH dependence: Optimum pH is 6.95 and 7.05 with lysoplasmenyl-ethanolamine and lysoplasmenyl-choline as substrates, respectively.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ether lipid metabolic process Inferred from direct assay Ref.3. Source: UniProtKB
Cellular_component	cytoplasmic part Inferred from direct assay Ref.3. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from direct assay Ref.3. Source: UniProtKB
Molecular_function	alkenylglycerophosphocholine hydrolase activity Inferred from direct assay Ref.3. Source: UniProtKB alkenylglycerophosphoethanolamine hydrolase activity Inferred from direct assay Ref.3. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Lysoplasmalogenase		PRO_0000201841
Regions
	Transmembrane	21 – 41	21	Helical; Potential
	Transmembrane	47 – 67	21	Helical; Potential
	Transmembrane	85 – 105	21	Helical; Potential
	Transmembrane	109 – 129	21	Helical; Potential
	Transmembrane	147 – 167	21	Helical; Potential
	Transmembrane	172 – 192	21	Helical; Potential
	Transmembrane	198 – 218	21	Helical; Potential
Natural variations
	Natural variant	176	1	A → T. Corresponds to variant rs35608872 [ dbSNP | Ensembl ].		VAR_052343
	Natural variant	199	1	H → R. Ref.2 Corresponds to variant rs4644955 [ dbSNP | Ensembl ].		VAR_052344

Sequences

	Sequence		Length	Mass (Da)
	Q8N661 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: BD5344268F221275	FASTA	226	24,352
	10 20 30 40 50 60 MDAGKAGQTL KTHCSAQRPD VCRWLSPFIL SCCVYFCLWI PEDQLSWFAA LVKCLPVLCL 70 80 90 100 110 120 AGFLWVMSPS GGYTQLLQGA LVCSAVGDAC LIWPAAFVPG MAAFATAHLL YVWAFGFSPL 130 140 150 160 170 180 QPGLLLLIIL APGPYLSLVL QHLEPDMVLP VAAYGLILMA MLWRGLAQGG SAGWGALLFT 190 200 210 220 LSDGVLAWDT FAQPLPHAHL VIMTTYYAAQ LLITLSALRS PVPKTD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-199. Tissue: Placenta.
[3]	"Purification, identification, and cloning of lysoplasmalogenase, the enzyme that catalyzes hydrolysis of the vinyl ether bond of lysoplasmalogen." Wu L.C., Pfeiffer D.R., Calhoon E.A., Madiai F., Marcucci G., Liu S., Jurkowitz M.S. J. Biol. Chem. 286:24916-24930(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, DIMERIZATION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC010327 Genomic DNA. No translation available. BC023000 mRNA. Translation: AAH23000.1.
IPI	IPI00297119.
RefSeq	NP_776165.3. NM_173804.4.
UniGene	Hs.135215.
3D structure databases
ProteinModelPortal	Q8N661.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N661. 1 interaction.
STRING	9606.ENSP00000321038.
Polymorphism databases
DMDM	296452936.
Proteomic databases
PRIDE	Q8N661.
Protocols and materials databases
DNASU	255043.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000327042; ENSP00000321038; ENSG00000180089.
GeneID	255043.
KEGG	hsa:255043.
UCSC	uc002qjt.3. human.
Organism-specific databases
CTD	255043.
GeneCards	GC19M055738.
H-InvDB	HIX0174302. HIX0202856.
HGNC	HGNC:28448. TMEM86B.
neXtProt	NX_Q8N661.
PharmGKB	PA142670738.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG74425.
HOGENOM	HOG000204433.
HOVERGEN	HBG055092.
InParanoid	Q8N661.
OMA	DLFVFGL.
OrthoDB	EOG4Z36FN.
PhylomeDB	Q8N661.
Gene expression databases
Bgee	Q8N661.
CleanEx	HS_TMEM86B.
Genevestigator	Q8N661.
GermOnline	ENSG00000180089. Homo sapiens.
Family and domain databases
InterPro	IPR012506. YhhN. [Graphical view]
Pfam	PF07947. YhhN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	255043.
NextBio	92454.

Entry information

Entry name

TM86B_HUMAN

Accession

Primary (citable) accession number: Q8N661

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	May 18, 2010
Last modified:	April 3, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.