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Q8N661 (TM86B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysoplasmalogenase

EC=3.3.2.2
EC=3.3.2.5
Alternative name(s):
Transmembrane protein 86B
Gene names
Name:TMEM86B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length226 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Enzyme catalyzing the degradation of lysoplasmalogen. Lysoplasmalogens are formed by the hydrolysis of the abundant membrane glycerophospholipids plasmalogens. May control the respective levels of plasmalogens and lysoplasmalogens in cells and modulate cell membrane properties. Ref.3

Catalytic activity

1-(1-alkenyl)-sn-glycero-3-phosphocholine + H2O = an aldehyde + sn-glycero-3-phosphocholine. Ref.3

1-(1-alkenyl)-sn-glycero-3-phosphoethanolamine + H2O = an aldehyde + sn-glycero-3-phosphoethanolamine. Ref.3

Enzyme regulation

Competitively inhibited by lysophosphatidic acid. Ref.3

Subunit structure

Homodimer Probable. Ref.3

Subcellular location

Membrane; Multi-pass membrane protein Potential. Cytoplasm Ref.3.

Sequence similarities

Belongs to the TMEM86 family.

Biophysicochemical properties

Kinetic parameters:

KM=45 µM for lysoplasmenyl-choline Ref.3

KM=53 µM for lysoplasmenyl-ethanolamine

pH dependence:

Optimum pH is 6.95 and 7.05 with lysoplasmenyl-ethanolamine and lysoplasmenyl-choline as substrates, respectively.

Ontologies

Keywords
   Cellular componentCytoplasm
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processether lipid metabolic process

Inferred from direct assay Ref.3. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from direct assay Ref.3. Source: UniProtKB

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Inferred from direct assay Ref.3. Source: UniProtKB

   Molecular_functionalkenylglycerophosphocholine hydrolase activity

Inferred from direct assay Ref.3. Source: UniProtKB

alkenylglycerophosphoethanolamine hydrolase activity

Inferred from direct assay Ref.3. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 226226Lysoplasmalogenase
PRO_0000201841

Regions

Transmembrane21 – 4121Helical; Potential
Transmembrane47 – 6721Helical; Potential
Transmembrane85 – 10521Helical; Potential
Transmembrane109 – 12921Helical; Potential
Transmembrane147 – 16721Helical; Potential
Transmembrane172 – 19221Helical; Potential
Transmembrane198 – 21821Helical; Potential

Natural variations

Natural variant1761A → T.
Corresponds to variant rs35608872 [ dbSNP | Ensembl ].
VAR_052343
Natural variant1991H → R. Ref.2
Corresponds to variant rs4644955 [ dbSNP | Ensembl ].
VAR_052344

Sequences

Sequence LengthMass (Da)Tools
Q8N661 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: BD5344268F221275

FASTA22624,352
        10         20         30         40         50         60 
MDAGKAGQTL KTHCSAQRPD VCRWLSPFIL SCCVYFCLWI PEDQLSWFAA LVKCLPVLCL 

        70         80         90        100        110        120 
AGFLWVMSPS GGYTQLLQGA LVCSAVGDAC LIWPAAFVPG MAAFATAHLL YVWAFGFSPL 

       130        140        150        160        170        180 
QPGLLLLIIL APGPYLSLVL QHLEPDMVLP VAAYGLILMA MLWRGLAQGG SAGWGALLFT 

       190        200        210        220 
LSDGVLAWDT FAQPLPHAHL VIMTTYYAAQ LLITLSALRS PVPKTD 

« Hide

References

« Hide 'large scale' references
[1]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-199.
Tissue: Placenta.
[3]"Purification, identification, and cloning of lysoplasmalogenase, the enzyme that catalyzes hydrolysis of the vinyl ether bond of lysoplasmalogen."
Wu L.C., Pfeiffer D.R., Calhoon E.A., Madiai F., Marcucci G., Liu S., Jurkowitz M.S.
J. Biol. Chem. 286:24916-24930(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, DIMERIZATION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC010327 Genomic DNA. No translation available.
BC023000 mRNA. Translation: AAH23000.1.
RefSeqNP_776165.3. NM_173804.4.
UniGeneHs.135215.

3D structure databases

ProteinModelPortalQ8N661.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid129071. 1 interaction.
IntActQ8N661. 1 interaction.

Polymorphism databases

DMDM296452936.

Proteomic databases

PRIDEQ8N661.

Protocols and materials databases

DNASU255043.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000327042; ENSP00000321038; ENSG00000180089.
GeneID255043.
KEGGhsa:255043.
UCSCuc002qjt.3. human.

Organism-specific databases

CTD255043.
GeneCardsGC19M055738.
H-InvDBHIX0174302.
HIX0202856.
HGNCHGNC:28448. TMEM86B.
HPAHPA057689.
neXtProtNX_Q8N661.
PharmGKBPA142670738.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG74425.
HOGENOMHOG000204433.
HOVERGENHBG055092.
InParanoidQ8N661.
OMASHLMILA.
OrthoDBEOG7J9VQD.
PhylomeDBQ8N661.
TreeFamTF324663.

Gene expression databases

BgeeQ8N661.
CleanExHS_TMEM86B.
GenevestigatorQ8N661.

Family and domain databases

InterProIPR012506. YhhN.
[Graphical view]
PfamPF07947. YhhN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi255043.
NextBio92454.
PROQ8N661.

Entry information

Entry nameTM86B_HUMAN
AccessionPrimary (citable) accession number: Q8N661
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: May 18, 2010
Last modified: March 19, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM