Q8N612 (F16A2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
FTS and Hook-interacting protein
Short name=FHIP

Alternative name(s):
Protein FAM160A2

Gene names

Name:	FAM160A2
Synonyms:	C11orf56, KIAA1759

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	972 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Ref.6
Subunit structure	Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. The FHF complex associates with the homotypic vesicular sorting complex (the HOPS complex). Ref.6
Sequence similarities	Belongs to the UPF0518 family.
Sequence caution	The sequence BAB21850.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein transport Transport
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	early endosome to late endosome transport Inferred from mutant phenotype Ref.6. Source: UniProtKB endosome organization Inferred from mutant phenotype Ref.6. Source: UniProtKB endosome to lysosome transport Inferred from mutant phenotype Ref.6. Source: UniProtKB lysosome organization Inferred from mutant phenotype Ref.6. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	FHF complex Inferred from direct assay Ref.6. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N612-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N612-2)

The sequence of this isoform differs from the canonical sequence as follows:
     479-479: G → GGPSRETGRREDITG

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8N612-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-75: Missing.
     757-780: VYVNFLLTGLVAQLACHPQPLLRS → ALSCSTPTWSSSPVSSPCCRCWAL
     781-972: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 972	972	FTS and Hook-interacting protein		PRO_0000253859
Amino acid modifications
	Modified residue	510	1	Phosphoserine By similarity
	Modified residue	516	1	Phosphoserine By similarity
	Modified residue	523	1	Phosphoserine By similarity
	Modified residue	859	1	Phosphoserine By similarity
	Modified residue	897	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	1 – 75	75	Missing in isoform 3.		VSP_021128
	Alternative sequence	479	1	G → GGPSRETGRREDITG in isoform 2.		VSP_021129
	Alternative sequence	757 – 780	24	VYVNF…PLLRS → ALSCSTPTWSSSPVSSPCCR CWAL in isoform 3.		VSP_021130
	Alternative sequence	781 – 972	192	Missing in isoform 3.		VSP_021131
	Natural variant	462	1	R → W. Corresponds to variant rs35932378 [ dbSNP | Ensembl ].		VAR_057805
	Natural variant	491	1	T → M. Ref.1 Ref.3 Corresponds to variant rs3750944 [ dbSNP | Ensembl ].		VAR_028739
	Natural variant	619	1	R → L. Corresponds to variant rs3750943 [ dbSNP | Ensembl ].		VAR_028740
	Natural variant	754	1	Q → H. Corresponds to variant rs11040808 [ dbSNP | Ensembl ].		VAR_028741
Experimental info
	Sequence conflict	95	1	P → S in CAB66657. Ref.4
	Sequence conflict	136	1	M → V in CAB66657. Ref.4
	Sequence conflict	186	1	C → Y in BAB15443. Ref.3
	Sequence conflict	503	1	A → V in CAB66657. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 7, 2009. Version 3. Checksum: F1CC45667E1B6811	FASTA	972	105,568
	10 20 30 40 50 60 MERMNWLSRL ASRGPGHRIP QGANLQTPVM ADPETCLMVF KNHWSQVVRI LERQGPRAAP 70 80 90 100 110 120 GGADDLSAVR NHTYQMLTLL AEDRAVPSAP TGPGPLLEFA LHEDLLTRVL TWQLQWDELG 130 140 150 160 170 180 DGVEERRAEQ LKLFEMLVSE ARQPLLRHGP VREALLTLLD ACGRPVPSSP ALDEGLVLLL 190 200 210 220 230 240 SQLCVCVAQE PSLLEFFLQP PPEPGAAPRL LLFSRLVPFV HREGTLGQQA RDALLLLMAL 250 260 270 280 290 300 SAGSPTVGRY IADHSYFCPV LATGLSALYS SLPRKIEVPG DDWHCLRRED WLGVPALALF 310 320 330 340 350 360 MSSLEFCNAV IQVAHPLVQK QLVDYIHNGF LVPVMGPALH KTSVEEMIAS TAYLELFLRS 370 380 390 400 410 420 ISEPALLRTF LRFLLLHRHD THTILDTLVA RIGSNSRLCM VSLSLFRTLL NLSCEDVLLQ 430 440 450 460 470 480 LVLRYLVPCN HVMLSQKPAV RDVDLYGRAA DKFLSLIPRC CRHHAPSPPR PEHASWARGP 490 500 510 520 530 540 GSPSVDSSSV TTVPRPSTPS RLALFLRQQS LGGSESPGPA PCSPGLSASP ASSPGRRPTP 550 560 570 580 590 600 AEEPGELEDN YLEYLREARR GVDRCVRACR TWSAPYDGER PSPEPSPFGS RTKKRSLLPE 610 620 630 640 650 660 EDRNNVGEGE EEELGRRGRA GGAGEGPGHL PPPQLNGVPG SWPEGAKKVR LVPKEGAGEL 670 680 690 700 710 720 LEGISEGMAG LEGFGQELRE LEVALSNGGT GSESPLEPPL PLEEEEAYES FTCPPEPPGP 730 740 750 760 770 780 FLSSPLRTLN QLPSQPFTGP FMAVLFAKLE NMLQNSVYVN FLLTGLVAQL ACHPQPLLRS 790 800 810 820 830 840 FLLNTNMVFQ PSVKSLLQVL GSVKNKIENF AASQEDFPAL LSKAKKYLIA RGKLDWAEGP 850 860 870 880 890 900 AAGPAPRRSD PLVKSRRPSL GELLLRHAHS PTRARQAAQL VLQPGRDGAG LGLSGGSPGA 910 920 930 940 950 960 STPVLLTRGG APERQGEALR VKNAVYCAVI FPEFLKELAA ISQAHAVTSP FLLETSEEGS 970 GPLISGCGPL NP
	Isoform 2 [UniParc]. Checksum: 18C2DACC61A49115	FASTA	986	107,081
	10 20 30 40 50 60 MERMNWLSRL ASRGPGHRIP QGANLQTPVM ADPETCLMVF KNHWSQVVRI LERQGPRAAP 70 80 90 100 110 120 GGADDLSAVR NHTYQMLTLL AEDRAVPSAP TGPGPLLEFA LHEDLLTRVL TWQLQWDELG 130 140 150 160 170 180 DGVEERRAEQ LKLFEMLVSE ARQPLLRHGP VREALLTLLD ACGRPVPSSP ALDEGLVLLL 190 200 210 220 230 240 SQLCVCVAQE PSLLEFFLQP PPEPGAAPRL LLFSRLVPFV HREGTLGQQA RDALLLLMAL 250 260 270 280 290 300 SAGSPTVGRY IADHSYFCPV LATGLSALYS SLPRKIEVPG DDWHCLRRED WLGVPALALF 310 320 330 340 350 360 MSSLEFCNAV IQVAHPLVQK QLVDYIHNGF LVPVMGPALH KTSVEEMIAS TAYLELFLRS 370 380 390 400 410 420 ISEPALLRTF LRFLLLHRHD THTILDTLVA RIGSNSRLCM VSLSLFRTLL NLSCEDVLLQ 430 440 450 460 470 480 LVLRYLVPCN HVMLSQKPAV RDVDLYGRAA DKFLSLIPRC CRHHAPSPPR PEHASWARGG 490 500 510 520 530 540 PSRETGRRED ITGPGSPSVD SSSVTTVPRP STPSRLALFL RQQSLGGSES PGPAPCSPGL 550 560 570 580 590 600 SASPASSPGR RPTPAEEPGE LEDNYLEYLR EARRGVDRCV RACRTWSAPY DGERPSPEPS 610 620 630 640 650 660 PFGSRTKKRS LLPEEDRNNV GEGEEEELGR RGRAGGAGEG PGHLPPPQLN GVPGSWPEGA 670 680 690 700 710 720 KKVRLVPKEG AGELLEGISE GMAGLEGFGQ ELRELEVALS NGGTGSESPL EPPLPLEEEE 730 740 750 760 770 780 AYESFTCPPE PPGPFLSSPL RTLNQLPSQP FTGPFMAVLF AKLENMLQNS VYVNFLLTGL 790 800 810 820 830 840 VAQLACHPQP LLRSFLLNTN MVFQPSVKSL LQVLGSVKNK IENFAASQED FPALLSKAKK 850 860 870 880 890 900 YLIARGKLDW AEGPAAGPAP RRSDPLVKSR RPSLGELLLR HAHSPTRARQ AAQLVLQPGR 910 920 930 940 950 960 DGAGLGLSGG SPGASTPVLL TRGGAPERQG EALRVKNAVY CAVIFPEFLK ELAAISQAHA 970 980 VTSPFLLETS EEGSGPLISG CGPLNP
	Isoform 3 [UniParc]. Checksum: A1F02608EBBE8B11	FASTA	705	76,834
	10 20 30 40 50 60 MLTLLAEDRA VPSAPTGPGP LLEFALHEDL LTRVLTWQLQ WDELGDGVEE RRAEQLKLFE 70 80 90 100 110 120 MLVSEARQPL LRHGPVREAL LTLLDACGRP VPSSPALDEG LVLLLSQLCV CVAQEPSLLE 130 140 150 160 170 180 FFLQPPPEPG AAPRLLLFSR LVPFVHREGT LGQQARDALL LLMALSAGSP TVGRYIADHS 190 200 210 220 230 240 YFCPVLATGL SALYSSLPRK IEVPGDDWHC LRREDWLGVP ALALFMSSLE FCNAVIQVAH 250 260 270 280 290 300 PLVQKQLVDY IHNGFLVPVM GPALHKTSVE EMIASTAYLE LFLRSISEPA LLRTFLRFLL 310 320 330 340 350 360 LHRHDTHTIL DTLVARIGSN SRLCMVSLSL FRTLLNLSCE DVLLQLVLRY LVPCNHVMLS 370 380 390 400 410 420 QKPAVRDVDL YGRAADKFLS LIPRCCRHHA PSPPRPEHAS WARGPGSPSV DSSSVTTVPR 430 440 450 460 470 480 PSTPSRLALF LRQQSLGGSE SPGPAPCSPG LSASPASSPG RRPTPAEEPG ELEDNYLEYL 490 500 510 520 530 540 REARRGVDRC VRACRTWSAP YDGERPSPEP SPFGSRTKKR SLLPEEDRNN VGEGEEEELG 550 560 570 580 590 600 RRGRAGGAGE GPGHLPPPQL NGVPGSWPEG AKKVRLVPKE GAGELLEGIS EGMAGLEGFG 610 620 630 640 650 660 QELRELEVAL SNGGTGSESP LEPPLPLEEE EAYESFTCPP EPPGPFLSSP LRTLNQLPSQ 670 680 690 700 PFTGPFMAVL FAKLENMLQN SALSCSTPTW SSSPVSSPCC RCWAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Hattori A., Kondo Y., Okumura K., Ohara O. DNA Res. 7:347-355(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT MET-491.
[2]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT MET-491. Tissue: Small intestine.
[4]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Kidney.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[6]	"An FTS/Hook/p107(FHIP) complex interacts with and promotes endosomal clustering by the homotypic vacuolar protein sorting complex." Xu L., Sowa M.E., Chen J., Li X., Gygi S.P., Harper J.W. Mol. Biol. Cell 19:5059-5071(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FHF COMPLEX, FUNCTION, ASSOCIATION WITH THE HOPS COMPLEX.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-897, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB051546 mRNA. Translation: BAB21850.1. Different initiation. AK026318 mRNA. Translation: BAB15443.1. AL136723 mRNA. Translation: CAB66657.1. BC030825 mRNA. Translation: AAH30825.1.
IPI	IPI00149375. IPI00789519. IPI00798104.
RefSeq	NP_001092264.1. NM_001098794.1. NP_115503.2. NM_032127.3.
UniGene	Hs.501793.
3D structure databases
ProteinModelPortal	Q8N612.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N612. 4 interactions.
MINT	MINT-1459470.
STRING	9606.ENSP00000265978.
PTM databases
PhosphoSite	Q8N612.
Polymorphism databases
DMDM	251757473.
Proteomic databases
PaxDb	Q8N612.
PRIDE	Q8N612.
Protocols and materials databases
DNASU	84067.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000265978; ENSP00000265978; ENSG00000051009. ENST00000449352; ENSP00000416918; ENSG00000051009.
GeneID	84067.
KEGG	hsa:84067.
UCSC	uc001mck.4. human. uc001mcl.4. human.
Organism-specific databases
CTD	84067.
GeneCards	GC11M006232.
HGNC	HGNC:25378. FAM160A2.
neXtProt	NX_Q8N612.
PharmGKB	PA162386769.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG318159.
HOGENOM	HOG000112444.
HOVERGEN	HBG107874.
InParanoid	Q8N612.
OMA	KLDWAEG.
Gene expression databases
ArrayExpress	Q8N612.
Bgee	Q8N612.
CleanEx	HS_FAM160A2.
Genevestigator	Q8N612.
GermOnline	ENSG00000051009. Homo sapiens.
Family and domain databases
InterPro	IPR019384. RetinoicA-induced_16-like. [Graphical view]
Pfam	PF10257. RAI16-like. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	FAM160A2. human.
GenomeRNAi	84067.
NextBio	73247.

Entry information

Entry name

F16A2_HUMAN

Accession

Primary (citable) accession number: Q8N612
Secondary accession number(s): Q9C0A4, Q9H0N3, Q9H624

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	July 7, 2009
Last modified:	April 3, 2013
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.