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Q8N5P1

- ZC3H8_HUMAN

UniProt

Q8N5P1 - ZC3H8_HUMAN

Protein

Zinc finger CCCH domain-containing protein 8

Gene

ZC3H8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (10 May 2004)
      Previous versions | rss
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    Functioni

    Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene By similarity. Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:23932780). Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.By similarity3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri191 – 21828C3H1-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri220 – 24728C3H1-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri248 – 27124C3H1-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. poly(A) RNA binding Source: UniProtKB
    3. sequence-specific DNA binding Source: UniProtKB
    4. sequence-specific DNA binding transcription factor activity Source: UniProtKB

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. negative regulation of T cell differentiation in thymus Source: UniProtKB
    3. negative regulation of transcription, DNA-templated Source: UniProtKB
    4. positive regulation of thymocyte apoptotic process Source: UniProtKB
    5. response to antibiotic Source: UniProtKB
    6. T cell homeostasis Source: UniProtKB
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Apoptosis, Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger CCCH domain-containing protein 8
    Gene namesi
    Name:ZC3H8
    Synonyms:ZC3HDC8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:30941. ZC3H8.

    Subcellular locationi

    Nucleus 1 Publication
    Note: Colocalizes with coilin in subnuclear cajal and histone locus bodies. Translocates in the LEC complex to cajal and histone locus bodies at snRNA genes in a ICE1-dependent manner. Associates to transcriptionally active chromatin at snRNA genes.

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134956342.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 291291Zinc finger CCCH domain-containing protein 8PRO_0000213903Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei32 – 321Phosphothreonine1 Publication
    Modified residuei77 – 771Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8N5P1.
    PaxDbiQ8N5P1.
    PRIDEiQ8N5P1.

    PTM databases

    PhosphoSiteiQ8N5P1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8N5P1.
    BgeeiQ8N5P1.
    CleanExiHS_ZC3H8.
    GenevestigatoriQ8N5P1.

    Organism-specific databases

    HPAiHPA034803.
    HPA034804.

    Interactioni

    Subunit structurei

    Component of the little elongation complex (LEC), at least composed of ELL (ELL, ELL2 or ELL3), ZC3H8, ICE1 and ICE2. Interacts with ICE1 (via C-terminus domain).1 Publication

    Protein-protein interaction databases

    BioGridi124116. 1 interaction.
    IntActiQ8N5P1. 1 interaction.
    STRINGi9606.ENSP00000386488.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8N5P1.
    SMRiQ8N5P1. Positions 194-283.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The N-terminal region and all three C3H1-type zinc fingers are necessary to induce transcriptional repression.1 Publication

    Sequence similaritiesi

    Contains 3 C3H1-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri191 – 21828C3H1-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri220 – 24728C3H1-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri248 – 27124C3H1-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG287629.
    HOGENOMiHOG000231732.
    HOVERGENiHBG057407.
    InParanoidiQ8N5P1.
    OMAiYCKFSHA.
    OrthoDBiEOG79GT5K.
    PhylomeDBiQ8N5P1.
    TreeFamiTF318143.

    Family and domain databases

    Gene3Di4.10.1000.10. 1 hit.
    InterProiIPR000571. Znf_CCCH.
    [Graphical view]
    PfamiPF00642. zf-CCCH. 2 hits.
    [Graphical view]
    SMARTiSM00356. ZnF_C3H1. 3 hits.
    [Graphical view]
    PROSITEiPS50103. ZF_C3H1. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8N5P1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDFENLFSKP PNPALGKTAT DSDERIDDEI DTEVEETQEE KIKLECEQIP    50
    KKFRHSAISP KSSLHRKSRS KDYDVYSDND ICSQESEDNF AKELQQYIQA 100
    REMANAAQPE ESTKKEGVKD TPQAAKQKNK NLKAGHKNGK QKKMKRKWPG 150
    PGNKGSNALL RNSGSQEEDG KPKEKQQHLS QAFINQHTVE RKGKQICKYF 200
    LERKCIKGDQ CKFDHDAEIE KKKEMCKFYV QGYCTRGENC LYLHNEYPCK 250
    FYHTGTKCYQ GEYCKFSHAP LTPETQELLA KVLDTEKKSC K 291
    Length:291
    Mass (Da):33,576
    Last modified:May 10, 2004 - v2
    Checksum:i5DFDA42280916034
    GO

    Sequence cautioni

    The sequence AAH32001.1 differs from that shown. Reason: Frameshift at position 291.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti233 – 2353YCT → FCS in AAK13496. 1 PublicationCurated
    Sequence conflicti265 – 2651K → Q in AAK13496. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti240 – 2401C → W.
    Corresponds to variant rs35782954 [ dbSNP | Ensembl ].
    VAR_057489

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF334161 mRNA. Translation: AAK13496.1.
    BC032001 mRNA. Translation: AAH32001.1. Frameshift.
    CCDSiCCDS46392.1.
    RefSeqiNP_115883.2. NM_032494.2.
    UniGeneiHs.418416.

    Genome annotation databases

    EnsembliENST00000272570; ENSP00000272570; ENSG00000144161.
    ENST00000409573; ENSP00000386488; ENSG00000144161.
    GeneIDi84524.
    KEGGihsa:84524.
    UCSCiuc021vmw.1. human.

    Polymorphism databases

    DMDMi47117585.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF334161 mRNA. Translation: AAK13496.1 .
    BC032001 mRNA. Translation: AAH32001.1 . Frameshift.
    CCDSi CCDS46392.1.
    RefSeqi NP_115883.2. NM_032494.2.
    UniGenei Hs.418416.

    3D structure databases

    ProteinModelPortali Q8N5P1.
    SMRi Q8N5P1. Positions 194-283.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 124116. 1 interaction.
    IntActi Q8N5P1. 1 interaction.
    STRINGi 9606.ENSP00000386488.

    PTM databases

    PhosphoSitei Q8N5P1.

    Polymorphism databases

    DMDMi 47117585.

    Proteomic databases

    MaxQBi Q8N5P1.
    PaxDbi Q8N5P1.
    PRIDEi Q8N5P1.

    Protocols and materials databases

    DNASUi 84524.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000272570 ; ENSP00000272570 ; ENSG00000144161 .
    ENST00000409573 ; ENSP00000386488 ; ENSG00000144161 .
    GeneIDi 84524.
    KEGGi hsa:84524.
    UCSCi uc021vmw.1. human.

    Organism-specific databases

    CTDi 84524.
    GeneCardsi GC02M112973.
    H-InvDB HIX0002377.
    HGNCi HGNC:30941. ZC3H8.
    HPAi HPA034803.
    HPA034804.
    neXtProti NX_Q8N5P1.
    PharmGKBi PA134956342.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG287629.
    HOGENOMi HOG000231732.
    HOVERGENi HBG057407.
    InParanoidi Q8N5P1.
    OMAi YCKFSHA.
    OrthoDBi EOG79GT5K.
    PhylomeDBi Q8N5P1.
    TreeFami TF318143.

    Miscellaneous databases

    GenomeRNAii 84524.
    NextBioi 74347.
    PROi Q8N5P1.

    Gene expression databases

    ArrayExpressi Q8N5P1.
    Bgeei Q8N5P1.
    CleanExi HS_ZC3H8.
    Genevestigatori Q8N5P1.

    Family and domain databases

    Gene3Di 4.10.1000.10. 1 hit.
    InterProi IPR000571. Znf_CCCH.
    [Graphical view ]
    Pfami PF00642. zf-CCCH. 2 hits.
    [Graphical view ]
    SMARTi SM00356. ZnF_C3H1. 3 hits.
    [Graphical view ]
    PROSITEi PS50103. ZF_C3H1. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mammalian Ssb proteins related to ovarian development and differentiation."
      Zhuang D.Z., Gunnarsson D., Toffia O., Lind M., Lundgren P., Selstam G.
      Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Skin.
    3. "Regulation of thymocyte homeostasis by Fliz1."
      Hwang E.S., Ho I.-C.
      Immunology 106:464-469(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Transcriptional regulation of GATA-3 by an intronic regulatory region and fetal liver zinc finger protein 1."
      Hwang E.S., Choi A., Ho I.-C.
      J. Immunol. 169:248-253(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic kidney.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "The little elongation complex functions at initiation and elongation phases of snRNA gene transcription."
      Hu D., Smith E.R., Garruss A.S., Mohaghegh N., Varberg J.M., Lin C., Jackson J., Gao X., Saraf A., Florens L., Washburn M.P., Eissenberg J.C., Shilatifard A.
      Mol. Cell 51:493-505(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE LEC COMPLEX, INTERACTION WITH ICE1.

    Entry informationi

    Entry nameiZC3H8_HUMAN
    AccessioniPrimary (citable) accession number: Q8N5P1
    Secondary accession number(s): Q9BZ75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3