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Protein

Zinc finger CCCH domain-containing protein 8

Gene

ZC3H8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (By similarity). Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:23932780). Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.By similarity3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri191 – 21828C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri220 – 24728C3H1-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri248 – 27124C3H1-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • poly(A) RNA binding Source: UniProtKB
  • sequence-specific DNA binding Source: UniProtKB
  • sequence-specific DNA binding transcription factor activity Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • negative regulation of T cell differentiation in thymus Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of thymocyte apoptotic process Source: UniProtKB
  • positive regulation of transcription from RNA polymerase III promoter Source: UniProtKB
  • response to antibiotic Source: UniProtKB
  • snRNA transcription from RNA polymerase III promoter Source: UniProtKB
  • snRNA transcription from RNA polymerase II promoter Source: UniProtKB
  • T cell homeostasis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger CCCH domain-containing protein 8
Gene namesi
Name:ZC3H8
Synonyms:ZC3HDC8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:30941. ZC3H8.

Subcellular locationi

GO - Cellular componenti

  • Cajal body Source: UniProtKB
  • histone locus body Source: UniProtKB
  • nucleus Source: UniProtKB
  • transcriptionally active chromatin Source: UniProtKB
  • transcription elongation factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134956342.

Polymorphism and mutation databases

BioMutaiZC3H8.
DMDMi47117585.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Zinc finger CCCH domain-containing protein 8PRO_0000213903Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321Phosphothreonine1 Publication
Modified residuei77 – 771Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8N5P1.
PaxDbiQ8N5P1.
PRIDEiQ8N5P1.

PTM databases

PhosphoSiteiQ8N5P1.

Expressioni

Gene expression databases

BgeeiQ8N5P1.
CleanExiHS_ZC3H8.
GenevestigatoriQ8N5P1.

Organism-specific databases

HPAiHPA034803.
HPA034804.

Interactioni

Subunit structurei

Component of the little elongation complex (LEC), at least composed of ELL (ELL, ELL2 or ELL3), ZC3H8, ICE1 and ICE2. Interacts with ICE1 (via C-terminus domain).1 Publication

Protein-protein interaction databases

BioGridi124116. 9 interactions.
IntActiQ8N5P1. 1 interaction.
STRINGi9606.ENSP00000386488.

Structurei

3D structure databases

ProteinModelPortaliQ8N5P1.
SMRiQ8N5P1. Positions 217-283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region and all three C3H1-type zinc fingers are necessary to induce transcriptional repression.1 Publication

Sequence similaritiesi

Contains 3 C3H1-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri191 – 21828C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri220 – 24728C3H1-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri248 – 27124C3H1-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG287629.
GeneTreeiENSGT00530000063288.
HOGENOMiHOG000231732.
HOVERGENiHBG057407.
InParanoidiQ8N5P1.
OMAiYCKFSHA.
OrthoDBiEOG79GT5K.
PhylomeDBiQ8N5P1.
TreeFamiTF318143.

Family and domain databases

Gene3Di4.10.1000.10. 1 hit.
InterProiIPR000571. Znf_CCCH.
[Graphical view]
PfamiPF00642. zf-CCCH. 2 hits.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 3 hits.
[Graphical view]
PROSITEiPS50103. ZF_C3H1. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8N5P1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFENLFSKP PNPALGKTAT DSDERIDDEI DTEVEETQEE KIKLECEQIP
60 70 80 90 100
KKFRHSAISP KSSLHRKSRS KDYDVYSDND ICSQESEDNF AKELQQYIQA
110 120 130 140 150
REMANAAQPE ESTKKEGVKD TPQAAKQKNK NLKAGHKNGK QKKMKRKWPG
160 170 180 190 200
PGNKGSNALL RNSGSQEEDG KPKEKQQHLS QAFINQHTVE RKGKQICKYF
210 220 230 240 250
LERKCIKGDQ CKFDHDAEIE KKKEMCKFYV QGYCTRGENC LYLHNEYPCK
260 270 280 290
FYHTGTKCYQ GEYCKFSHAP LTPETQELLA KVLDTEKKSC K
Length:291
Mass (Da):33,576
Last modified:May 10, 2004 - v2
Checksum:i5DFDA42280916034
GO

Sequence cautioni

The sequence AAH32001.1 differs from that shown. Reason: Frameshift at position 291. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti233 – 2353YCT → FCS in AAK13496 (Ref. 1) Curated
Sequence conflicti265 – 2651K → Q in AAK13496 (Ref. 1) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti240 – 2401C → W.
Corresponds to variant rs35782954 [ dbSNP | Ensembl ].
VAR_057489

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334161 mRNA. Translation: AAK13496.1.
BC032001 mRNA. Translation: AAH32001.1. Frameshift.
CCDSiCCDS46392.1.
RefSeqiNP_115883.2. NM_032494.2.
UniGeneiHs.418416.

Genome annotation databases

EnsembliENST00000272570; ENSP00000272570; ENSG00000144161.
ENST00000409573; ENSP00000386488; ENSG00000144161.
GeneIDi84524.
KEGGihsa:84524.
UCSCiuc021vmw.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334161 mRNA. Translation: AAK13496.1.
BC032001 mRNA. Translation: AAH32001.1. Frameshift.
CCDSiCCDS46392.1.
RefSeqiNP_115883.2. NM_032494.2.
UniGeneiHs.418416.

3D structure databases

ProteinModelPortaliQ8N5P1.
SMRiQ8N5P1. Positions 217-283.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124116. 9 interactions.
IntActiQ8N5P1. 1 interaction.
STRINGi9606.ENSP00000386488.

PTM databases

PhosphoSiteiQ8N5P1.

Polymorphism and mutation databases

BioMutaiZC3H8.
DMDMi47117585.

Proteomic databases

MaxQBiQ8N5P1.
PaxDbiQ8N5P1.
PRIDEiQ8N5P1.

Protocols and materials databases

DNASUi84524.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000272570; ENSP00000272570; ENSG00000144161.
ENST00000409573; ENSP00000386488; ENSG00000144161.
GeneIDi84524.
KEGGihsa:84524.
UCSCiuc021vmw.1. human.

Organism-specific databases

CTDi84524.
GeneCardsiGC02M112973.
H-InvDBHIX0002377.
HGNCiHGNC:30941. ZC3H8.
HPAiHPA034803.
HPA034804.
neXtProtiNX_Q8N5P1.
PharmGKBiPA134956342.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG287629.
GeneTreeiENSGT00530000063288.
HOGENOMiHOG000231732.
HOVERGENiHBG057407.
InParanoidiQ8N5P1.
OMAiYCKFSHA.
OrthoDBiEOG79GT5K.
PhylomeDBiQ8N5P1.
TreeFamiTF318143.

Miscellaneous databases

ChiTaRSiZC3H8. human.
GenomeRNAii84524.
NextBioi74347.
PROiQ8N5P1.

Gene expression databases

BgeeiQ8N5P1.
CleanExiHS_ZC3H8.
GenevestigatoriQ8N5P1.

Family and domain databases

Gene3Di4.10.1000.10. 1 hit.
InterProiIPR000571. Znf_CCCH.
[Graphical view]
PfamiPF00642. zf-CCCH. 2 hits.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 3 hits.
[Graphical view]
PROSITEiPS50103. ZF_C3H1. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mammalian Ssb proteins related to ovarian development and differentiation."
    Zhuang D.Z., Gunnarsson D., Toffia O., Lind M., Lundgren P., Selstam G.
    Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skin.
  3. "Regulation of thymocyte homeostasis by Fliz1."
    Hwang E.S., Ho I.-C.
    Immunology 106:464-469(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Transcriptional regulation of GATA-3 by an intronic regulatory region and fetal liver zinc finger protein 1."
    Hwang E.S., Choi A., Ho I.-C.
    J. Immunol. 169:248-253(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "The little elongation complex functions at initiation and elongation phases of snRNA gene transcription."
    Hu D., Smith E.R., Garruss A.S., Mohaghegh N., Varberg J.M., Lin C., Jackson J., Gao X., Saraf A., Florens L., Washburn M.P., Eissenberg J.C., Shilatifard A.
    Mol. Cell 51:493-505(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE LEC COMPLEX, INTERACTION WITH ICE1.

Entry informationi

Entry nameiZC3H8_HUMAN
AccessioniPrimary (citable) accession number: Q8N5P1
Secondary accession number(s): Q9BZ75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: May 27, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.