Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8N5P1 (ZC3H8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger CCCH domain-containing protein 8
Gene names
Name:ZC3H8
Synonyms:ZC3HDC8
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene By similarity. Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis. Ref.3 Ref.4

Subcellular location

Nucleus By similarity.

Domain

The N-terminal region and all three C3H1-type zinc fingers are necessary to induce transcriptional repression.

Sequence similarities

Contains 3 C3H1-type zinc fingers.

Sequence caution

The sequence AAH32001.1 differs from that shown. Reason: Frameshift at position 291.

Ontologies

Keywords
   Biological processApoptosis
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityPolymorphism
   DomainRepeat
Zinc-finger
   LigandMetal-binding
RNA-binding
Zinc
   Molecular functionRepressor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processT cell homeostasis

Inferred from mutant phenotype Ref.3. Source: UniProtKB

apoptotic process

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of T cell differentiation in thymus

Inferred from mutant phenotype Ref.3. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from direct assay Ref.4. Source: UniProtKB

positive regulation of thymocyte apoptotic process

Inferred from mutant phenotype Ref.3. Source: UniProtKB

response to antibiotic

Inferred from direct assay Ref.3. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

poly(A) RNA binding

Inferred from direct assay PubMed 22658674PubMed 22681889. Source: UniProtKB

sequence-specific DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from mutant phenotype Ref.3. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Zinc finger CCCH domain-containing protein 8
PRO_0000213903

Regions

Zinc finger191 – 21828C3H1-type 1
Zinc finger220 – 24728C3H1-type 2
Zinc finger248 – 27124C3H1-type 3

Amino acid modifications

Modified residue321Phosphothreonine Ref.8
Modified residue771Phosphoserine Ref.5 Ref.6

Natural variations

Natural variant2401C → W.
Corresponds to variant rs35782954 [ dbSNP | Ensembl ].
VAR_057489

Experimental info

Sequence conflict233 – 2353YCT → FCS in AAK13496. Ref.1
Sequence conflict2651K → Q in AAK13496. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8N5P1 [UniParc].

Last modified May 10, 2004. Version 2.
Checksum: 5DFDA42280916034

FASTA29133,576
        10         20         30         40         50         60 
MDFENLFSKP PNPALGKTAT DSDERIDDEI DTEVEETQEE KIKLECEQIP KKFRHSAISP 

        70         80         90        100        110        120 
KSSLHRKSRS KDYDVYSDND ICSQESEDNF AKELQQYIQA REMANAAQPE ESTKKEGVKD 

       130        140        150        160        170        180 
TPQAAKQKNK NLKAGHKNGK QKKMKRKWPG PGNKGSNALL RNSGSQEEDG KPKEKQQHLS 

       190        200        210        220        230        240 
QAFINQHTVE RKGKQICKYF LERKCIKGDQ CKFDHDAEIE KKKEMCKFYV QGYCTRGENC 

       250        260        270        280        290 
LYLHNEYPCK FYHTGTKCYQ GEYCKFSHAP LTPETQELLA KVLDTEKKSC K 

« Hide

References

« Hide 'large scale' references
[1]"Mammalian Ssb proteins related to ovarian development and differentiation."
Zhuang D.Z., Gunnarsson D., Toffia O., Lind M., Lundgren P., Selstam G.
Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skin.
[3]"Regulation of thymocyte homeostasis by Fliz1."
Hwang E.S., Ho I.-C.
Immunology 106:464-469(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Transcriptional regulation of GATA-3 by an intronic regulatory region and fetal liver zinc finger protein 1."
Hwang E.S., Choi A., Ho I.-C.
J. Immunol. 169:248-253(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic kidney.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF334161 mRNA. Translation: AAK13496.1.
BC032001 mRNA. Translation: AAH32001.1. Frameshift.
CCDSCCDS46392.1.
RefSeqNP_115883.2. NM_032494.2.
UniGeneHs.418416.

3D structure databases

ProteinModelPortalQ8N5P1.
SMRQ8N5P1. Positions 194-283.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124116. 1 interaction.
IntActQ8N5P1. 1 interaction.
STRING9606.ENSP00000386488.

PTM databases

PhosphoSiteQ8N5P1.

Polymorphism databases

DMDM47117585.

Proteomic databases

MaxQBQ8N5P1.
PaxDbQ8N5P1.
PRIDEQ8N5P1.

Protocols and materials databases

DNASU84524.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000272570; ENSP00000272570; ENSG00000144161.
ENST00000409573; ENSP00000386488; ENSG00000144161.
GeneID84524.
KEGGhsa:84524.
UCSCuc021vmw.1. human.

Organism-specific databases

CTD84524.
GeneCardsGC02M112973.
H-InvDBHIX0002377.
HGNCHGNC:30941. ZC3H8.
HPAHPA034803.
HPA034804.
neXtProtNX_Q8N5P1.
PharmGKBPA134956342.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG287629.
HOGENOMHOG000231732.
HOVERGENHBG057407.
InParanoidQ8N5P1.
OMAYCKFSHA.
OrthoDBEOG79GT5K.
PhylomeDBQ8N5P1.
TreeFamTF318143.

Gene expression databases

ArrayExpressQ8N5P1.
BgeeQ8N5P1.
CleanExHS_ZC3H8.
GenevestigatorQ8N5P1.

Family and domain databases

Gene3D4.10.1000.10. 1 hit.
InterProIPR000571. Znf_CCCH.
[Graphical view]
PfamPF00642. zf-CCCH. 2 hits.
[Graphical view]
SMARTSM00356. ZnF_C3H1. 3 hits.
[Graphical view]
PROSITEPS50103. ZF_C3H1. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi84524.
NextBio74347.
PROQ8N5P1.

Entry information

Entry nameZC3H8_HUMAN
AccessionPrimary (citable) accession number: Q8N5P1
Secondary accession number(s): Q9BZ75
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: July 9, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM