Q8N5P1 (ZC3H8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger CCCH domain-containing protein 8

Gene names

Name:	ZC3H8
Synonyms:	ZC3HDC8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene By similarity. Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis. Ref.3 Ref.4
Subcellular location	Nucleus By similarity.
Domain	The N-terminal region and all three C3H1-type zinc fingers are necessary to induce transcriptional repression.
Sequence similarities	Contains 3 C3H1-type zinc fingers.
Sequence caution	The sequence AAH32001.1 differs from that shown. Reason: Frameshift at position 291.

Ontologies

Keywords
Biological process	Apoptosis Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell homeostasis Inferred from mutant phenotype Ref.3. Source: UniProtKB apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of T cell differentiation in thymus Inferred from mutant phenotype Ref.3. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.4. Source: UniProtKB positive regulation of thymocyte apoptotic process Inferred from mutant phenotype Ref.3. Source: UniProtKB response to antibiotic Inferred from direct assay Ref.3. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding Inferred from sequence or structural similarity. Source: UniProtKB sequence-specific DNA binding transcription factor activity Inferred from mutant phenotype Ref.3. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Zinc finger CCCH domain-containing protein 8		PRO_0000213903
Regions
	Zinc finger	191 – 218	28	C3H1-type 1
	Zinc finger	220 – 247	28	C3H1-type 2
	Zinc finger	248 – 271	24	C3H1-type 3
Amino acid modifications
	Modified residue	32	1	Phosphothreonine Ref.7
	Modified residue	77	1	Phosphoserine Ref.5 Ref.6
Natural variations
	Natural variant	240	1	C → W. Corresponds to variant rs35782954 [ dbSNP | Ensembl ].		VAR_057489
Experimental info
	Sequence conflict	233 – 235	3	YCT → FCS in AAK13496. Ref.1
	Sequence conflict	265	1	K → Q in AAK13496. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8N5P1 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: 5DFDA42280916034	FASTA	291	33,576
	10 20 30 40 50 60 MDFENLFSKP PNPALGKTAT DSDERIDDEI DTEVEETQEE KIKLECEQIP KKFRHSAISP 70 80 90 100 110 120 KSSLHRKSRS KDYDVYSDND ICSQESEDNF AKELQQYIQA REMANAAQPE ESTKKEGVKD 130 140 150 160 170 180 TPQAAKQKNK NLKAGHKNGK QKKMKRKWPG PGNKGSNALL RNSGSQEEDG KPKEKQQHLS 190 200 210 220 230 240 QAFINQHTVE RKGKQICKYF LERKCIKGDQ CKFDHDAEIE KKKEMCKFYV QGYCTRGENC 250 260 270 280 290 LYLHNEYPCK FYHTGTKCYQ GEYCKFSHAP LTPETQELLA KVLDTEKKSC K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mammalian Ssb proteins related to ovarian development and differentiation." Zhuang D.Z., Gunnarsson D., Toffia O., Lind M., Lundgren P., Selstam G. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[3]	"Regulation of thymocyte homeostasis by Fliz1." Hwang E.S., Ho I.-C. Immunology 106:464-469(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Transcriptional regulation of GATA-3 by an intronic regulatory region and fetal liver zinc finger protein 1." Hwang E.S., Choi A., Ho I.-C. J. Immunol. 169:248-253(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-32, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF334161 mRNA. Translation: AAK13496.1. BC032001 mRNA. Translation: AAH32001.1. Frameshift.
IPI	IPI00307760.
RefSeq	NP_115883.2. NM_032494.2.
UniGene	Hs.418416.
3D structure databases
ProteinModelPortal	Q8N5P1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N5P1. 1 interaction.
STRING	9606.ENSP00000386488.
PTM databases
PhosphoSite	Q8N5P1.
Polymorphism databases
DMDM	47117585.
Proteomic databases
PaxDb	Q8N5P1.
PRIDE	Q8N5P1.
Protocols and materials databases
DNASU	84524.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000272570; ENSP00000272570; ENSG00000144161. ENST00000409573; ENSP00000386488; ENSG00000144161.
GeneID	84524.
KEGG	hsa:84524.
UCSC	uc021vmw.1. human.
Organism-specific databases
CTD	84524.
GeneCards	GC02M112973.
H-InvDB	HIX0002377.
HGNC	HGNC:30941. ZC3H8.
HPA	HPA034804.
neXtProt	NX_Q8N5P1.
PharmGKB	PA134956342.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG287629.
HOGENOM	HOG000231732.
HOVERGEN	HBG057407.
InParanoid	Q8N5P1.
OMA	PKKFRHS.
OrthoDB	EOG454907.
Gene expression databases
ArrayExpress	Q8N5P1.
Bgee	Q8N5P1.
CleanEx	HS_ZC3H8.
Genevestigator	Q8N5P1.
GermOnline	ENSG00000144161. Homo sapiens.
Family and domain databases
Gene3D	4.10.1000.10. 1 hit.
InterPro	IPR000571. Znf_CCCH. [Graphical view]
Pfam	PF00642. zf-CCCH. 2 hits. [Graphical view]
SMART	SM00356. ZnF_C3H1. 3 hits. [Graphical view]
PROSITE	PS50103. ZF_C3H1. 3 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	84524.
NextBio	74347.

Entry information

Entry name

ZC3H8_HUMAN

Accession

Primary (citable) accession number: Q8N5P1
Secondary accession number(s): Q9BZ75

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 10, 2004
Last modified:	May 29, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.