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Q8N5P1

- ZC3H8_HUMAN

UniProt

Q8N5P1 - ZC3H8_HUMAN

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Protein

Zinc finger CCCH domain-containing protein 8

Gene

ZC3H8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (By similarity). Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:23932780). Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.By similarity3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri191 – 21828C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri220 – 24728C3H1-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri248 – 27124C3H1-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. poly(A) RNA binding Source: UniProtKB
  3. sequence-specific DNA binding Source: UniProtKB
  4. sequence-specific DNA binding transcription factor activity Source: UniProtKB

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. negative regulation of T cell differentiation in thymus Source: UniProtKB
  3. negative regulation of transcription, DNA-templated Source: UniProtKB
  4. positive regulation of thymocyte apoptotic process Source: UniProtKB
  5. positive regulation of transcription from RNA polymerase III promoter Source: UniProtKB
  6. response to antibiotic Source: UniProtKB
  7. snRNA transcription from RNA polymerase III promoter Source: UniProtKB
  8. snRNA transcription from RNA polymerase II promoter Source: UniProtKB
  9. T cell homeostasis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger CCCH domain-containing protein 8
Gene namesi
Name:ZC3H8
Synonyms:ZC3HDC8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:30941. ZC3H8.

Subcellular locationi

Nucleus 1 Publication
Note: Colocalizes with coilin in subnuclear cajal and histone locus bodies. Translocates in the LEC complex to cajal and histone locus bodies at snRNA genes in a ICE1-dependent manner. Associates to transcriptionally active chromatin at snRNA genes.

GO - Cellular componenti

  1. Cajal body Source: UniProtKB
  2. histone locus body Source: UniProtKB
  3. nucleus Source: UniProtKB
  4. transcriptionally active chromatin Source: UniProtKB
  5. transcription elongation factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134956342.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Zinc finger CCCH domain-containing protein 8PRO_0000213903Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321Phosphothreonine1 Publication
Modified residuei77 – 771Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8N5P1.
PaxDbiQ8N5P1.
PRIDEiQ8N5P1.

PTM databases

PhosphoSiteiQ8N5P1.

Expressioni

Gene expression databases

BgeeiQ8N5P1.
CleanExiHS_ZC3H8.
ExpressionAtlasiQ8N5P1. baseline and differential.
GenevestigatoriQ8N5P1.

Organism-specific databases

HPAiHPA034803.
HPA034804.

Interactioni

Subunit structurei

Component of the little elongation complex (LEC), at least composed of ELL (ELL, ELL2 or ELL3), ZC3H8, ICE1 and ICE2. Interacts with ICE1 (via C-terminus domain).1 Publication

Protein-protein interaction databases

BioGridi124116. 11 interactions.
IntActiQ8N5P1. 1 interaction.
STRINGi9606.ENSP00000386488.

Structurei

3D structure databases

ProteinModelPortaliQ8N5P1.
SMRiQ8N5P1. Positions 217-283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region and all three C3H1-type zinc fingers are necessary to induce transcriptional repression.1 Publication

Sequence similaritiesi

Contains 3 C3H1-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri191 – 21828C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri220 – 24728C3H1-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri248 – 27124C3H1-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG287629.
GeneTreeiENSGT00530000063288.
HOGENOMiHOG000231732.
HOVERGENiHBG057407.
InParanoidiQ8N5P1.
OMAiYCKFSHA.
OrthoDBiEOG79GT5K.
PhylomeDBiQ8N5P1.
TreeFamiTF318143.

Family and domain databases

Gene3Di4.10.1000.10. 1 hit.
InterProiIPR000571. Znf_CCCH.
[Graphical view]
PfamiPF00642. zf-CCCH. 2 hits.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 3 hits.
[Graphical view]
PROSITEiPS50103. ZF_C3H1. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8N5P1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDFENLFSKP PNPALGKTAT DSDERIDDEI DTEVEETQEE KIKLECEQIP
60 70 80 90 100
KKFRHSAISP KSSLHRKSRS KDYDVYSDND ICSQESEDNF AKELQQYIQA
110 120 130 140 150
REMANAAQPE ESTKKEGVKD TPQAAKQKNK NLKAGHKNGK QKKMKRKWPG
160 170 180 190 200
PGNKGSNALL RNSGSQEEDG KPKEKQQHLS QAFINQHTVE RKGKQICKYF
210 220 230 240 250
LERKCIKGDQ CKFDHDAEIE KKKEMCKFYV QGYCTRGENC LYLHNEYPCK
260 270 280 290
FYHTGTKCYQ GEYCKFSHAP LTPETQELLA KVLDTEKKSC K
Length:291
Mass (Da):33,576
Last modified:May 10, 2004 - v2
Checksum:i5DFDA42280916034
GO

Sequence cautioni

The sequence AAH32001.1 differs from that shown. Reason: Frameshift at position 291.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti233 – 2353YCT → FCS in AAK13496. 1 PublicationCurated
Sequence conflicti265 – 2651K → Q in AAK13496. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti240 – 2401C → W.
Corresponds to variant rs35782954 [ dbSNP | Ensembl ].
VAR_057489

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF334161 mRNA. Translation: AAK13496.1.
BC032001 mRNA. Translation: AAH32001.1. Frameshift.
CCDSiCCDS46392.1.
RefSeqiNP_115883.2. NM_032494.2.
UniGeneiHs.418416.

Genome annotation databases

EnsembliENST00000272570; ENSP00000272570; ENSG00000144161.
ENST00000409573; ENSP00000386488; ENSG00000144161.
ENST00000615791; ENSP00000483976; ENSG00000144161.
GeneIDi84524.
KEGGihsa:84524.
UCSCiuc021vmw.1. human.

Polymorphism databases

DMDMi47117585.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF334161 mRNA. Translation: AAK13496.1 .
BC032001 mRNA. Translation: AAH32001.1 . Frameshift.
CCDSi CCDS46392.1.
RefSeqi NP_115883.2. NM_032494.2.
UniGenei Hs.418416.

3D structure databases

ProteinModelPortali Q8N5P1.
SMRi Q8N5P1. Positions 217-283.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 124116. 11 interactions.
IntActi Q8N5P1. 1 interaction.
STRINGi 9606.ENSP00000386488.

PTM databases

PhosphoSitei Q8N5P1.

Polymorphism databases

DMDMi 47117585.

Proteomic databases

MaxQBi Q8N5P1.
PaxDbi Q8N5P1.
PRIDEi Q8N5P1.

Protocols and materials databases

DNASUi 84524.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000272570 ; ENSP00000272570 ; ENSG00000144161 .
ENST00000409573 ; ENSP00000386488 ; ENSG00000144161 .
ENST00000615791 ; ENSP00000483976 ; ENSG00000144161 .
GeneIDi 84524.
KEGGi hsa:84524.
UCSCi uc021vmw.1. human.

Organism-specific databases

CTDi 84524.
GeneCardsi GC02M112973.
H-InvDB HIX0002377.
HGNCi HGNC:30941. ZC3H8.
HPAi HPA034803.
HPA034804.
neXtProti NX_Q8N5P1.
PharmGKBi PA134956342.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG287629.
GeneTreei ENSGT00530000063288.
HOGENOMi HOG000231732.
HOVERGENi HBG057407.
InParanoidi Q8N5P1.
OMAi YCKFSHA.
OrthoDBi EOG79GT5K.
PhylomeDBi Q8N5P1.
TreeFami TF318143.

Miscellaneous databases

GenomeRNAii 84524.
NextBioi 74347.
PROi Q8N5P1.

Gene expression databases

Bgeei Q8N5P1.
CleanExi HS_ZC3H8.
ExpressionAtlasi Q8N5P1. baseline and differential.
Genevestigatori Q8N5P1.

Family and domain databases

Gene3Di 4.10.1000.10. 1 hit.
InterProi IPR000571. Znf_CCCH.
[Graphical view ]
Pfami PF00642. zf-CCCH. 2 hits.
[Graphical view ]
SMARTi SM00356. ZnF_C3H1. 3 hits.
[Graphical view ]
PROSITEi PS50103. ZF_C3H1. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mammalian Ssb proteins related to ovarian development and differentiation."
    Zhuang D.Z., Gunnarsson D., Toffia O., Lind M., Lundgren P., Selstam G.
    Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skin.
  3. "Regulation of thymocyte homeostasis by Fliz1."
    Hwang E.S., Ho I.-C.
    Immunology 106:464-469(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Transcriptional regulation of GATA-3 by an intronic regulatory region and fetal liver zinc finger protein 1."
    Hwang E.S., Choi A., Ho I.-C.
    J. Immunol. 169:248-253(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "The little elongation complex functions at initiation and elongation phases of snRNA gene transcription."
    Hu D., Smith E.R., Garruss A.S., Mohaghegh N., Varberg J.M., Lin C., Jackson J., Gao X., Saraf A., Florens L., Washburn M.P., Eissenberg J.C., Shilatifard A.
    Mol. Cell 51:493-505(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE LEC COMPLEX, INTERACTION WITH ICE1.

Entry informationi

Entry nameiZC3H8_HUMAN
AccessioniPrimary (citable) accession number: Q8N5P1
Secondary accession number(s): Q9BZ75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: October 29, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3