Q8N5I3 (KCNRG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium channel regulatory protein
Short name=Potassium channel regulator

Alternative name(s):
Protein CLLD4

Gene names

Name:	KCNRG
Synonyms:	CLLD4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits potassium fluxes in cells. May regulate Kv1 family channel proteins by retaining a fraction of channels in endomembranes. Ref.1 Ref.8
Subcellular location	Endoplasmic reticulum Ref.8.
Tissue specificity	Ubiquitous in normal tissues and expressed in some tumor tissues. Ref.1
Sequence similarities	Contains 1 BTB (POZ) domain.
Sequence caution	The sequence CAD38633.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell voltage-gated potassium channel complex Inferred from electronic annotation. Source: InterPro
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct voltage-gated potassium channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-745755,EBI-745755

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N5I3-1)

Also known as: B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N5I3-2)

Also known as: A;

The sequence of this isoform differs from the canonical sequence as follows:
     194-229: YVSIKPDNRKLANGTNVLGLLIDTLLKEGFHLVSTR → LVCNGVISAHHNLRLWGSSDSPASASRVAGITGMFL
     230-272: Missing.

Isoform 3 (identifier: Q8N5I3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     195-213: VSIKPDNRKLANGTNVLGL → FVLCSISLVYQFVMFSLKT
     214-272: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Potassium channel regulatory protein		PRO_0000238945
Regions
	Domain	5 – 106	102	BTB
Amino acid modifications
	Modified residue	208	1	Phosphothreonine Ref.7
Natural variations
	Alternative sequence	194 – 229	36	YVSIK…LVSTR → LVCNGVISAHHNLRLWGSSD SPASASRVAGITGMFL in isoform 2.		VSP_019018
	Alternative sequence	195 – 213	19	VSIKP…NVLGL → FVLCSISLVYQFVMFSLKT in isoform 3.		VSP_019019
	Alternative sequence	214 – 272	59	Missing in isoform 3.		VSP_019020
	Alternative sequence	230 – 272	43	Missing in isoform 2.		VSP_019021

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (B) [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 5018F55980F0BBA5	FASTA	272	31,048
	10 20 30 40 50 60 MSSQELVTLN VGGKIFTTRF STIKQFPASR LARMLDGRDQ EFKMVGGQIF VDRDGDLFSF 70 80 90 100 110 120 ILDFLRTHQL LLPTEFSDYL RLQREALFYE LRSLVDLLNP YLLQPRPALV EVHFLSRNTQ 130 140 150 160 170 180 AFFRVFGSCS KTIEMLTGRI TVFTEQPSAP TWNGNFFPPQ MTLLPLPPQR PSYHDLVFQC 190 200 210 220 230 240 GSDSTTDNQT GVRYVSIKPD NRKLANGTNV LGLLIDTLLK EGFHLVSTRT VSSEDKTECY 250 260 270 SFERIKSPEV LITNETPKPE TIIIPEQSQI KK
	Isoform 2 (A) [UniParc]. Checksum: 9065D19F89DEFA58	FASTA	229	25,857
	10 20 30 40 50 60 MSSQELVTLN VGGKIFTTRF STIKQFPASR LARMLDGRDQ EFKMVGGQIF VDRDGDLFSF 70 80 90 100 110 120 ILDFLRTHQL LLPTEFSDYL RLQREALFYE LRSLVDLLNP YLLQPRPALV EVHFLSRNTQ 130 140 150 160 170 180 AFFRVFGSCS KTIEMLTGRI TVFTEQPSAP TWNGNFFPPQ MTLLPLPPQR PSYHDLVFQC 190 200 210 220 GSDSTTDNQT GVRLVCNGVI SAHHNLRLWG SSDSPASASR VAGITGMFL
	Isoform 3 [UniParc]. Checksum: 27150A2D2CFBD9F4	FASTA	213	24,520
	10 20 30 40 50 60 MSSQELVTLN VGGKIFTTRF STIKQFPASR LARMLDGRDQ EFKMVGGQIF VDRDGDLFSF 70 80 90 100 110 120 ILDFLRTHQL LLPTEFSDYL RLQREALFYE LRSLVDLLNP YLLQPRPALV EVHFLSRNTQ 130 140 150 160 170 180 AFFRVFGSCS KTIEMLTGRI TVFTEQPSAP TWNGNFFPPQ MTLLPLPPQR PSYHDLVFQC 190 200 210 GSDSTTDNQT GVRYFVLCSI SLVYQFVMFS LKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new human gene KCNRG encoding potassium channel regulating protein is a cancer suppressor gene candidate located in 13q14.3." Ivanov D.V., Tyazhelova T.V., Lemonnier L., Kononenko N., Pestova A.A., Nikitin E.A., Prevarskaya N., Skryma R., Panchin Y.V., Yankovsky N.K., Baranova A.V. FEBS Lett. 539:156-160(2003) [PubMed: 12650944] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, FUNCTION.
[2]	"Cloning of spliced CLLD4 gene transcripts." Skoldberg F., Alimohammadi M., Hedstrand H., Kampe O. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Lung.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Heart.
[4]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Lung.
[7]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-208, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"Potassium channel regulator KCNRG regulates surface expression of Shaker-type potassium channels." Usman H., Mathew M.K. Biochem. Biophys. Res. Commun. 391:1301-1305(2010) [PubMed: 19968958] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY129653 mRNA. Translation: AAN06090.1. AY129654 mRNA. Translation: AAN06091.1. AY169387 mRNA. Translation: AAO11777.1. AY169388 mRNA. Translation: AAO11778.1. AY190921 mRNA. Translation: AAO27464.1. AY190922 mRNA. Translation: AAO27465.1. AL832695 mRNA. Translation: CAD38633.1. Different initiation. AL137060 Genomic DNA. Translation: CAM17621.1. AL137060 Genomic DNA. Translation: CAM17622.1. CH471075 Genomic DNA. Translation: EAX08849.1. CH471075 Genomic DNA. Translation: EAX08850.1. BC020887 mRNA. Translation: AAH20887.1. BC032343 mRNA. Translation: AAH32343.1.
IPI	IPI00166859. IPI00216656. IPI00759536.
RefSeq	NP_001007279.1. NM_001007278.1. NP_005789.2. NM_005798.3. NP_434698.1. NM_052811.2. NP_775876.1. NM_173605.1. NP_955751.1. NM_199464.1. NP_998755.1. NM_213590.1.
UniGene	Hs.436922. Hs.660161.
3D structure databases
ProteinModelPortal	Q8N5I3.
SMR	Q8N5I3. Positions 4-100.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N5I3. 9 interactions.
MINT	MINT-1460322.
STRING	Q8N5I3.
PTM databases
PhosphoSite	Q8N5I3.
Polymorphism databases
DMDM	74729005.
Proteomic databases
PRIDE	Q8N5I3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000312942; ENSP00000324191; ENSG00000198553.
GeneID	10206. 283518.
KEGG	hsa:10206. hsa:283518.
UCSC	uc001vdt.1. human. uc001vdu.1. human.
Organism-specific databases
CTD	10206. 283518.
GeneCards	GC13P050589.
HGNC	HGNC:18893. KCNRG.
HPA	HPA001741.
MIM	607947. gene.
neXtProt	NX_Q8N5I3.
PharmGKB	PA134924183.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00600000084079.
HOGENOM	HBG278573.
HOVERGEN	HBG079982.
InParanoid	Q8N5I3.
OMA	ECYSFER.
PhylomeDB	Q8N5I3.
Gene expression databases
Bgee	Q8N5I3.
CleanEx	HS_KCNRG.
Genevestigator	Q8N5I3.
GermOnline	ENSG00000198553. Homo sapiens.
Family and domain databases
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR003131. K_chnl_volt-dep_Kv_tetra. [Graphical view]
Gene3D	G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit.
KO	K12003.
Pfam	PF02214. K_tetra. 1 hit. [Graphical view]
SMART	SM00225. BTB. 1 hit. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
PROSITE	PS50097. BTB. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	94005.
SOURCE	Search...

Entry information

Entry name

KCNRG_HUMAN

Accession

Primary (citable) accession number: Q8N5I3
Secondary accession number(s): A2A2X9 , Q0P6D0, Q8IU75, Q8N3Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.