Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Arrestin domain-containing protein 1

Gene

ARRDC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as an adapter recruiting ubiquitin-protein ligases to their specific substrates (PubMed:23886940, PubMed:27462458). Through an ubiquitination-dependent mechanism plays for instance a role in the incorporation of SLC11A2 into extracellular vesicles (PubMed:27462458). More generally, plays a role in the extracellular transport of proteins between cells through the release in the extracellular space of microvesicles (PubMed:22315426). By participating to the ITCH-mediated ubiquitination and subsequent degradation of NOTCH1, negatively regulates the NOTCH signaling pathway (PubMed:23886940).3 Publications

GO - Molecular functioni

  • arrestin family protein binding Source: UniProtKB
  • identical protein binding Source: UniProtKB
  • protein binding, bridging involved in substrate recognition for ubiquitination Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • extracellular transport Source: UniProtKB
  • extracellular vesicle biogenesis Source: UniProtKB
  • negative regulation of Notch signaling pathway Source: UniProtKB
  • protein transport Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • signal transduction Source: InterPro
  • ubiquitin-dependent protein catabolic process Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Arrestin domain-containing protein 1Curated
Alternative name(s):
Alpha-arrestin 11 Publication
Gene namesi
Name:ARRDC1Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000197070.13.
HGNCiHGNC:28633. ARRDC1.
neXtProtiNX_Q8N5I2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi88F → L: Loss of localization to the cell membrane and extracellular vesicles. Localizes to the cytoplasm. 1 Publication1
Mutagenesisi180G → A: Decreased localization to the cell membrane and extracellular vesicles. 1 Publication1
Mutagenesisi191N → D: Decreased localization to the cell membrane and extracellular vesicles. 1 Publication1
Mutagenesisi310S → A: Decreased interaction with TSG101. No effect on localization to the cell membrane. Loss of localization to extracellular vesicles. 1 Publication1
Mutagenesisi403P → A: Loss of interaction with ITCH; when associated with A-416. 1 Publication1
Mutagenesisi416P → A: Loss of interaction with ITCH; when associated with A-403. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000197070.
PharmGKBiPA134938267.

Polymorphism and mutation databases

BioMutaiARRDC1.
DMDMi74751028.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002443451 – 433Arrestin domain-containing protein 1Add BLAST433

Post-translational modificationi

Ubiquitinated (PubMed:21191027, PubMed:22315426). Ubiquitination by WWP2; promotes localization to extracellular microvesicles (PubMed:22315426). Ubiquitinated by WWP1 (PubMed:21191027).2 Publications

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ8N5I2.
MaxQBiQ8N5I2.
PaxDbiQ8N5I2.
PeptideAtlasiQ8N5I2.
PRIDEiQ8N5I2.

PTM databases

iPTMnetiQ8N5I2.
PhosphoSitePlusiQ8N5I2.

Expressioni

Gene expression databases

BgeeiENSG00000197070.
CleanExiHS_ARRDC1.
ExpressionAtlasiQ8N5I2. baseline and differential.
GenevisibleiQ8N5I2. HS.

Organism-specific databases

HPAiHPA022915.
HPA044345.

Interactioni

Subunit structurei

Interacts (via PPxY motifs) with ITCH (via WW domains); the interaction is direct and participates to the recruitment of the ubiquitin-protein ligase ITCH to the NOTCH1 receptor (PubMed:21191027, PubMed:23886940). Interacts with ARRB1 and ARRB2; the interaction is direct (PubMed:23886940). Interacts with TSG101; may recruit TSG101 to the plasma membrane (PubMed:21191027, PubMed:22315426). Interacts (via PPxY motifs) with WWP2 (via WW domains); ubiquitinates ARRDC1 (PubMed:21191027, PubMed:22315426). Interacts with SLC11A2; controls the incorporation of SLC11A2 into extracellular vesicles through an ubiquitination-dependent mechanism (PubMed:27462458). Interacts with WWP1 (via WW domains) (PubMed:21191027). Interacts with NEDD4 (via WW domains) (PubMed:21191027). Interacts with ALIX (PubMed:21191027).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
WWP2O003083EBI-2339564,EBI-743923

GO - Molecular functioni

  • arrestin family protein binding Source: UniProtKB
  • identical protein binding Source: UniProtKB
  • protein binding, bridging involved in substrate recognition for ubiquitination Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi124970. 36 interactors.
IntActiQ8N5I2. 8 interactors.
STRINGi9606.ENSP00000360475.

Structurei

3D structure databases

ProteinModelPortaliQ8N5I2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi402 – 405PPxY motif 11 Publication4
Motifi415 – 418PPxY motif 21 Publication4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi290 – 416Pro-richAdd BLAST127

Domaini

The PPxY motifs mediate interaction with WW domain-containing ubiquitin-protein ligases.2 Publications

Sequence similaritiesi

Belongs to the arrestin family.Curated

Phylogenomic databases

eggNOGiKOG3780. Eukaryota.
ENOG41102NY. LUCA.
GeneTreeiENSGT00550000074356.
HOGENOMiHOG000231531.
HOVERGENiHBG059908.
InParanoidiQ8N5I2.
OMAiRWIYDVR.
OrthoDBiEOG091G07XG.
PhylomeDBiQ8N5I2.
TreeFamiTF313650.

Family and domain databases

Gene3Di2.60.40.640. 2 hits.
InterProiView protein in InterPro
IPR011021. Arrestin-like_N.
IPR014752. Arrestin_C.
IPR011022. Arrestin_C-like.
IPR014756. Ig_E-set.
PfamiView protein in Pfam
PF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
SMARTiView protein in SMART
SM01017. Arrestin_C. 2 hits.
SUPFAMiSSF81296. SSF81296. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8N5I2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRVQLFEIS LSHGRVVYSP GEPLAGTVRV RLGAPLPFRA IRVTCIGSCG
60 70 80 90 100
VSNKANDTAW VVEEGYFNSS LSLADKGSLP AGEHSFPFQF LLPATAPTSF
110 120 130 140 150
EGPFGKIVHQ VRAAIHTPRF SKDHKCSLVF YILSPLNLNS IPDIEQPNVA
160 170 180 190 200
SATKKFSYKL VKTGSVVLTA STDLRGYVVG QALQLHADVE NQSGKDTSPV
210 220 230 240 250
VASLLQKVSY KAKRWIHDVR TIAEVEGAGV KAWRRAQWHE QILVPALPQS
260 270 280 290 300
ALPGCSLIHI DYYLQVSLKA PEATVTLPVF IGNIAVNHAP VSPRPGLGLP
310 320 330 340 350
PGAPPLVVPS APPQEEAEAE AAAGGPHFLD PVFLSTKSHS QRQPLLATLS
360 370 380 390 400
SVPGAPEPCP QDGSPASHPL HPPLCISTGA TVPYFAEGSG GPVPTTSTLI
410 420 430
LPPEYSSWGY PYEAPPSYEQ SCGGVEPSLT PES
Length:433
Mass (Da):45,981
Last modified:October 1, 2002 - v1
Checksum:i5B52A79EE27B5DDB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048335363G → C. Corresponds to variant dbSNP:rs35018943Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC032346 mRNA. Translation: AAH32346.1.
AL365502 Genomic DNA. No translation available.
CCDSiCCDS7049.1.
RefSeqiNP_689498.1. NM_152285.3.
UniGeneiHs.12999.

Genome annotation databases

EnsembliENST00000371421; ENSP00000360475; ENSG00000197070.
GeneIDi92714.
KEGGihsa:92714.
UCSCiuc004cns.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiARRD1_HUMAN
AccessioniPrimary (citable) accession number: Q8N5I2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome