Q8N5H7 (SH2D3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SH2 domain-containing protein 3C

Alternative name(s):
Novel SH2-containing protein 3
SH2 domain-containing Eph receptor-binding protein 1
Short name=SHEP1

Gene names

Name:	SH2D3C
Synonyms:	NSP3
ORF Names:	UNQ272/PRO309/PRO34088

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	860 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Eph receptor-binding protein which may be a positive regulator of TCR signaling. Binding to BCAR1 is required to induce membrane ruffling and promote EGF-dependent cell migration By similarity.
Subunit structure	Interacts with PTK2B. Interacts with NEDD9/HEF1 By similarity. Interacts (via C-terminus) with BCAR1/CAS.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity. Note: Associated with the membrane when EGF-stimulated. Found at ruffling membranes By similarity.
Tissue specificity	Ubiquitously expressed. Ref.1
Domain	The C-terminal Cdc25-homology/Ras-GEF domain adopts a closed conformation rendering it incapable of carrying out canonical exchange factor function, this closed conformation is required for interaction with BCAR1.
Sequence similarities	Contains 1 Ras-GEF domain. Contains 1 SH2 domain.
Sequence caution	The sequence AAQ88456.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	SH2 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	JNK cascade Traceable author statement Ref.1. Source: ProtInc small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	SH3/SH2 adaptor activity Traceable author statement Ref.1. Source: ProtInc guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N5H7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N5H7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-172: MTEGTKKTSK...RPPRDVHSER → MTAVGRRCPALGSRG

Isoform 3 (identifier: Q8N5H7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-354: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q8N5H7-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-172: MTEGTKKTSK...RPPRDVHSER → MAPALPSPHK...GRCPLLLCGG

Note: No experimental confirmation available.

Isoform 5 (identifier: Q8N5H7-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-185: MTEGTKKTSK...EPEAGSDYVK → MTERCSLWSALSAAACCFYRGSFVQ
     186-186: F → VQF

Note: No experimental confirmation available.

Isoform 6 (identifier: Q8N5H7-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1-185: MTEGTKKTSK...EPEAGSDYVK → MTERCSLWSALSAAACCFYRGSFVQ

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 860

860

SH2 domain-containing protein 3C

PRO_0000228833

Regions

Domain

220 – 319

100

SH2

Domain

586 – 854

269

Ras-GEF

Amino acid modifications

Modified residue

278

1

Phosphotyrosine By similarity

Modified residue

283

1

Phosphotyrosine By similarity

Modified residue

793

1

Phosphotyrosine By similarity

Natural variations

Alternative sequence

1 – 354

354

Missing in isoform 3.

VSP_017706

Alternative sequence

1 – 185

185

MTEGT…SDYVK → MTERCSLWSALSAAACCFYR GSFVQ in isoform 5 and isoform 6.

VSP_044581

Alternative sequence

1 – 172

172

MTEGT…VHSER → MTAVGRRCPALGSRG in isoform 2.

VSP_017707

Alternative sequence

1 – 172

172

MTEGT…VHSER → MAPALPSPHKAVARPELLLD TAAHSGKLKAPDGGEGAECA GHNGGPSWGVGQGQSQEPSR QGIPQAPWLVSWQRRGFPPS SFCPGPLRTEKLRAGRCPLL LCGG in isoform 4.

VSP_017708

Alternative sequence

186

1

F → VQF in isoform 5.

VSP_044582

Natural variant

23

1

L → F.
Corresponds to variant rs10760500 [ dbSNP | Ensembl ].

VAR_051352

Experimental info

Sequence conflict

445

1

R → C in AAD28246. Ref.1

Sequence conflict

445

1

R → C in AAQ89948. Ref.2

Sequence conflict

723

1

K → E in BAF84082. Ref.3

Secondary structure

1

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860

Helix Strand Turn

Details...

Helix

555 – 557

3

Beta strand

561 – 563

3

Helix

572 – 583

12

Helix

587 – 601

15

Beta strand

604 – 606

3

Helix

609 – 615

7

Beta strand

616 – 618

3

Helix

620 – 623

4

Helix

627 – 629

3

Helix

630 – 652

23

Helix

658 – 677

20

Helix

682 – 692

11

Helix

695 – 698

4

Helix

701 – 710

10

Helix

712 – 720

9

Helix

722 – 730

9

Helix

738 – 740

3

Helix

747 – 752

6

Helix

773 – 787

15

Helix

790 – 800

11

Turn

801 – 803

3

Helix

808 – 813

6

Helix

816 – 824

9

Helix

828 – 830

3

Helix

833 – 851

19

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 62598F2F643147C5	FASTA	860	94,411
	10 20 30 40 50 60 MTEGTKKTSK KFKFFKFKGF GSLSNLPRSF TLRRSSASIS RQSHLEPDTF EATQDDMVTV 70 80 90 100 110 120 PKSPPAYARS SDMYSHMGTM PRPSIKKAQN SQAARQAQEA GPKPNLVPGG VPDPPGLEAA 130 140 150 160 170 180 KEVMVKATGP LEDTPAMEPN PSAVEVDPIR KPEVPTGDVE EERPPRDVHS ERAAGEPEAG 190 200 210 220 230 240 SDYVKFSKEK YILDSSPEKL HKELEEELKL SSTDLRSHAW YHGRIPREVS ETLVQRNGDF 250 260 270 280 290 300 LIRDSLTSLG DYVLTCRWRN QALHFKINKV VVKAGESYTH IQYLFEQESF DHVPALVRYH 310 320 330 340 350 360 VGSRKAVSEQ SGAIIYCPVN RTFPLRYLEA SYGLGQGSSK PASPVSPSGP KGSHMKRRSV 370 380 390 400 410 420 TMTDGLTADK VTRSDGCPTS TSLPRPRDSI RSCALSMDQI PDLHSPMSPI SESPSSPAYS 430 440 450 460 470 480 TVTRVHAAPA APSATALPAS PVARRSSEPQ LCPGSAPKTH GESDKGPHTS PSHTLGKASP 490 500 510 520 530 540 SPSLSSYSDP DSGHYCQLQP PVRGSREWAA TETSSQQARS YGERLKELSE NGAPEGDWGK 550 560 570 580 590 600 TFTVPIVEVT SSFNPATFQS LLIPRDNRPL EVGLLRKVKE LLAEVDARTL ARHVTKVDCL 610 620 630 640 650 660 VARILGVTKE MQTLMGVRWG MELLTLPHGR QLRLDLLERF HTMSIMLAVD ILGCTGSAEE 670 680 690 700 710 720 RAALLHKTIQ LAAELRGTMG NMFSFAAVMG ALDMAQISRL EQTWVTLRQR HTEGAILYEK 730 740 750 760 770 780 KLKPFLKSLN EGKEGPPLSN TTFPHVLPLI TLLECDSAPP EGPEPWGSTE HGVEVVLAHL 790 800 810 820 830 840 EAARTVAHHG GLYHTNAEVK LQGFQARPEL LEVFSTEFQM RLLWGSQGAS SSQARRYEKF 850 860 DKVLTALSHK LEPAVRSSEL
	Isoform 2 [UniParc]. Checksum: 005B0EA32F7CA869	FASTA	703	77,141
	10 20 30 40 50 60 MTAVGRRCPA LGSRGAAGEP EAGSDYVKFS KEKYILDSSP EKLHKELEEE LKLSSTDLRS 70 80 90 100 110 120 HAWYHGRIPR EVSETLVQRN GDFLIRDSLT SLGDYVLTCR WRNQALHFKI NKVVVKAGES 130 140 150 160 170 180 YTHIQYLFEQ ESFDHVPALV RYHVGSRKAV SEQSGAIIYC PVNRTFPLRY LEASYGLGQG 190 200 210 220 230 240 SSKPASPVSP SGPKGSHMKR RSVTMTDGLT ADKVTRSDGC PTSTSLPRPR DSIRSCALSM 250 260 270 280 290 300 DQIPDLHSPM SPISESPSSP AYSTVTRVHA APAAPSATAL PASPVARRSS EPQLCPGSAP 310 320 330 340 350 360 KTHGESDKGP HTSPSHTLGK ASPSPSLSSY SDPDSGHYCQ LQPPVRGSRE WAATETSSQQ 370 380 390 400 410 420 ARSYGERLKE LSENGAPEGD WGKTFTVPIV EVTSSFNPAT FQSLLIPRDN RPLEVGLLRK 430 440 450 460 470 480 VKELLAEVDA RTLARHVTKV DCLVARILGV TKEMQTLMGV RWGMELLTLP HGRQLRLDLL 490 500 510 520 530 540 ERFHTMSIML AVDILGCTGS AEERAALLHK TIQLAAELRG TMGNMFSFAA VMGALDMAQI 550 560 570 580 590 600 SRLEQTWVTL RQRHTEGAIL YEKKLKPFLK SLNEGKEGPP LSNTTFPHVL PLITLLECDS 610 620 630 640 650 660 APPEGPEPWG STEHGVEVVL AHLEAARTVA HHGGLYHTNA EVKLQGFQAR PELLEVFSTE 670 680 690 700 FQMRLLWGSQ GASSSQARRY EKFDKVLTAL SHKLEPAVRS SEL
	Isoform 3 [UniParc]. Checksum: 6A525DAC855CF389	FASTA	506	55,191
	10 20 30 40 50 60 MKRRSVTMTD GLTADKVTRS DGCPTSTSLP RPRDSIRSCA LSMDQIPDLH SPMSPISESP 70 80 90 100 110 120 SSPAYSTVTR VHAAPAAPSA TALPASPVAR RSSEPQLCPG SAPKTHGESD KGPHTSPSHT 130 140 150 160 170 180 LGKASPSPSL SSYSDPDSGH YCQLQPPVRG SREWAATETS SQQARSYGER LKELSENGAP 190 200 210 220 230 240 EGDWGKTFTV PIVEVTSSFN PATFQSLLIP RDNRPLEVGL LRKVKELLAE VDARTLARHV 250 260 270 280 290 300 TKVDCLVARI LGVTKEMQTL MGVRWGMELL TLPHGRQLRL DLLERFHTMS IMLAVDILGC 310 320 330 340 350 360 TGSAEERAAL LHKTIQLAAE LRGTMGNMFS FAAVMGALDM AQISRLEQTW VTLRQRHTEG 370 380 390 400 410 420 AILYEKKLKP FLKSLNEGKE GPPLSNTTFP HVLPLITLLE CDSAPPEGPE PWGSTEHGVE 430 440 450 460 470 480 VVLAHLEAAR TVAHHGGLYH TNAEVKLQGF QARPELLEVF STEFQMRLLW GSQGASSSQA 490 500 RRYEKFDKVL TALSHKLEPA VRSSEL
	Isoform 4 [UniParc]. Checksum: 7633DB25062F150C	FASTA	792	86,408
	10 20 30 40 50 60 MAPALPSPHK AVARPELLLD TAAHSGKLKA PDGGEGAECA GHNGGPSWGV GQGQSQEPSR 70 80 90 100 110 120 QGIPQAPWLV SWQRRGFPPS SFCPGPLRTE KLRAGRCPLL LCGGAAGEPE AGSDYVKFSK 130 140 150 160 170 180 EKYILDSSPE KLHKELEEEL KLSSTDLRSH AWYHGRIPRE VSETLVQRNG DFLIRDSLTS 190 200 210 220 230 240 LGDYVLTCRW RNQALHFKIN KVVVKAGESY THIQYLFEQE SFDHVPALVR YHVGSRKAVS 250 260 270 280 290 300 EQSGAIIYCP VNRTFPLRYL EASYGLGQGS SKPASPVSPS GPKGSHMKRR SVTMTDGLTA 310 320 330 340 350 360 DKVTRSDGCP TSTSLPRPRD SIRSCALSMD QIPDLHSPMS PISESPSSPA YSTVTRVHAA 370 380 390 400 410 420 PAAPSATALP ASPVARRSSE PQLCPGSAPK THGESDKGPH TSPSHTLGKA SPSPSLSSYS 430 440 450 460 470 480 DPDSGHYCQL QPPVRGSREW AATETSSQQA RSYGERLKEL SENGAPEGDW GKTFTVPIVE 490 500 510 520 530 540 VTSSFNPATF QSLLIPRDNR PLEVGLLRKV KELLAEVDAR TLARHVTKVD CLVARILGVT 550 560 570 580 590 600 KEMQTLMGVR WGMELLTLPH GRQLRLDLLE RFHTMSIMLA VDILGCTGSA EERAALLHKT 610 620 630 640 650 660 IQLAAELRGT MGNMFSFAAV MGALDMAQIS RLEQTWVTLR QRHTEGAILY EKKLKPFLKS 670 680 690 700 710 720 LNEGKEGPPL SNTTFPHVLP LITLLECDSA PPEGPEPWGS TEHGVEVVLA HLEAARTVAH 730 740 750 760 770 780 HGGLYHTNAE VKLQGFQARP ELLEVFSTEF QMRLLWGSQG ASSSQARRYE KFDKVLTALS 790 HKLEPAVRSS EL
	Isoform 5 [UniParc]. Checksum: F1B0521F92431081	FASTA	702	77,349
	10 20 30 40 50 60 MTERCSLWSA LSAAACCFYR GSFVQVQFSK EKYILDSSPE KLHKELEEEL KLSSTDLRSH 70 80 90 100 110 120 AWYHGRIPRE VSETLVQRNG DFLIRDSLTS LGDYVLTCRW RNQALHFKIN KVVVKAGESY 130 140 150 160 170 180 THIQYLFEQE SFDHVPALVR YHVGSRKAVS EQSGAIIYCP VNRTFPLRYL EASYGLGQGS 190 200 210 220 230 240 SKPASPVSPS GPKGSHMKRR SVTMTDGLTA DKVTRSDGCP TSTSLPRPRD SIRSCALSMD 250 260 270 280 290 300 QIPDLHSPMS PISESPSSPA YSTVTRVHAA PAAPSATALP ASPVARRSSE PQLCPGSAPK 310 320 330 340 350 360 THGESDKGPH TSPSHTLGKA SPSPSLSSYS DPDSGHYCQL QPPVRGSREW AATETSSQQA 370 380 390 400 410 420 RSYGERLKEL SENGAPEGDW GKTFTVPIVE VTSSFNPATF QSLLIPRDNR PLEVGLLRKV 430 440 450 460 470 480 KELLAEVDAR TLARHVTKVD CLVARILGVT KEMQTLMGVR WGMELLTLPH GRQLRLDLLE 490 500 510 520 530 540 RFHTMSIMLA VDILGCTGSA EERAALLHKT IQLAAELRGT MGNMFSFAAV MGALDMAQIS 550 560 570 580 590 600 RLEQTWVTLR QRHTEGAILY EKKLKPFLKS LNEGKEGPPL SNTTFPHVLP LITLLECDSA 610 620 630 640 650 660 PPEGPEPWGS TEHGVEVVLA HLEAARTVAH HGGLYHTNAE VKLQGFQARP ELLEVFSTEF 670 680 690 700 QMRLLWGSQG ASSSQARRYE KFDKVLTALS HKLEPAVRSS EL
	Isoform 6 [UniParc]. Checksum: 26650B429A943FF8	FASTA	700	77,122
	10 20 30 40 50 60 MTERCSLWSA LSAAACCFYR GSFVQFSKEK YILDSSPEKL HKELEEELKL SSTDLRSHAW 70 80 90 100 110 120 YHGRIPREVS ETLVQRNGDF LIRDSLTSLG DYVLTCRWRN QALHFKINKV VVKAGESYTH 130 140 150 160 170 180 IQYLFEQESF DHVPALVRYH VGSRKAVSEQ SGAIIYCPVN RTFPLRYLEA SYGLGQGSSK 190 200 210 220 230 240 PASPVSPSGP KGSHMKRRSV TMTDGLTADK VTRSDGCPTS TSLPRPRDSI RSCALSMDQI 250 260 270 280 290 300 PDLHSPMSPI SESPSSPAYS TVTRVHAAPA APSATALPAS PVARRSSEPQ LCPGSAPKTH 310 320 330 340 350 360 GESDKGPHTS PSHTLGKASP SPSLSSYSDP DSGHYCQLQP PVRGSREWAA TETSSQQARS 370 380 390 400 410 420 YGERLKELSE NGAPEGDWGK TFTVPIVEVT SSFNPATFQS LLIPRDNRPL EVGLLRKVKE 430 440 450 460 470 480 LLAEVDARTL ARHVTKVDCL VARILGVTKE MQTLMGVRWG MELLTLPHGR QLRLDLLERF 490 500 510 520 530 540 HTMSIMLAVD ILGCTGSAEE RAALLHKTIQ LAAELRGTMG NMFSFAAVMG ALDMAQISRL 550 560 570 580 590 600 EQTWVTLRQR HTEGAILYEK KLKPFLKSLN EGKEGPPLSN TTFPHVLPLI TLLECDSAPP 610 620 630 640 650 660 EGPEPWGSTE HGVEVVLAHL EAARTVAHHG GLYHTNAEVK LQGFQARPEL LEVFSTEFQM 670 680 690 700 RLLWGSQGAS SSQARRYEKF DKVLTALSHK LEPAVRSSEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NSP1 defines a novel family of adaptor proteins linking integrin and tyrosine kinase receptors to the c-Jun N-terminal kinase/stress-activated protein kinase signaling pathway." Lu Y., Brush J., Stewart T.A. J. Biol. Chem. 274:10047-10052(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Placenta.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 5 AND 6). Tissue: Brain.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Cervix.
[5]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Pancreas and Testis.
[7]	"NSP-Cas protein structures reveal a promiscuous interaction module in cell signaling." Mace P.D., Wallez Y., Dobaczewska M.K., Lee J.J., Robinson H., Pasquale E.B., Riedl S.J. Nat. Struct. Mol. Biol. 18:1381-1387(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 539-860 IN COMPLEX WITH BCAR1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF124251 mRNA. Translation: AAD28246.1.
AY358089 Transcribed RNA. Translation: AAQ88456.1. Different initiation.
AY358440 mRNA. Translation: AAQ89948.1.
AK056068 mRNA. Translation: BAG51614.1.
AK291393 mRNA. Translation: BAF84082.1.
BX647905 mRNA. Translation: CAI46101.1.
AL162586, AL162426 Genomic DNA. Translation: CAI39761.1.
AL162586, AL162426 Genomic DNA. Translation: CAI39763.1.
AL162586, AL162426 Genomic DNA. Translation: CAI39766.1.
AL162426, AL162586 Genomic DNA. Translation: CAI41177.1.
AL162426, AL162586 Genomic DNA. Translation: CAI41179.1.
AL162426, AL162586 Genomic DNA. Translation: CAI41182.1.
BC027962 mRNA. Translation: AAH27962.1.
BC032365 mRNA. Translation: AAH32365.1.

IPI

IPI00171093.
IPI00640927.
IPI00641965.
IPI00735492.
IPI00916634.

RefSeq

NP_001136004.1. NM_001142532.1.
NP_001136005.1. NM_001142533.1.
NP_001136006.1. NM_001142534.1.
NP_001239263.1. NM_001252334.1.
NP_005480.2. NM_005489.3.
NP_733745.1. NM_170600.2.

UniGene

Hs.306412.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3T6G	X-ray	2.50	A/C	539-860	[»]

ProteinModelPortal

Q8N5H7.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59443N.

IntAct

Q8N5H7. 1 interaction.

MINT

MINT-1427552.

STRING

9606.ENSP00000317817.

PTM databases

PhosphoSite

Q8N5H7.

Polymorphism databases

DMDM

74751027.

Proteomic databases

PaxDb

Q8N5H7.

PeptideAtlas

Q8N5H7.

PRIDE

Q8N5H7.

Protocols and materials databases

DNASU

10044.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000314830; ENSP00000317817; ENSG00000095370.
ENST00000373274; ENSP00000362371; ENSG00000095370.
ENST00000373277; ENSP00000362374; ENSG00000095370.
ENST00000420366; ENSP00000388536; ENSG00000095370.
ENST00000429553; ENSP00000394632; ENSG00000095370.

GeneID

10044.

KEGG

hsa:10044.

UCSC

uc004bry.3. human.
uc004brz.4. human.
uc004bsa.3. human.
uc010mxo.3. human.

Organism-specific databases

CTD

10044.

GeneCards

GC09M130501.

HGNC

HGNC:16884. SH2D3C.

HPA

HPA047586.

MIM

604722. gene.

neXtProt

NX_Q8N5H7.

PharmGKB

PA38191.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG149796.

HOGENOM

HOG000231595.

HOVERGEN

HBG053174.

InParanoid

Q8N5H7.

PhylomeDB

Q8N5H7.

Enzyme and pathway databases

SignaLink

Q8N5H7.

Gene expression databases

ArrayExpress

Q8N5H7.

Bgee

Q8N5H7.

CleanEx

HS_SH2D3C.

Genevestigator

Q8N5H7.

GermOnline

ENSG00000095370. Homo sapiens.

Family and domain databases

Gene3D

1.10.840.10. 1 hit.
3.30.505.10. 1 hit.

InterPro

IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
IPR000980. SH2.
[Graphical view]

Pfam

PF00617. RasGEF. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]

SMART

SM00147. RasGEF. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]

SUPFAM

SSF48366. Ras_GEF. 1 hit.

PROSITE

PS00720. RASGEF. False negative.
PS50009. RASGEF_CAT. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

10044.

NextBio

37937.

SOURCE

Search...

Entry information

Entry name

SH2D3_HUMAN

Accession

Primary (citable) accession number: Q8N5H7
Secondary accession number(s): A8K5S8 , E9PG48, Q5HYE5, Q5JU31, Q6UY42, Q8N6X3, Q9Y2X5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	October 1, 2002
Last modified:	May 29, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.