Q8N5G2 (MACOI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Macoilin

Alternative name(s):
Transmembrane protein 57

Gene names

Name:

TMEM57

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	664 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Nucleus membrane; Multi-pass membrane protein By similarity. Cell projection › axon By similarity. Note: Detected in the nucleus membrane of non-neuronal cells and in axonal outgrowths of neuronal cells By similarity.
Sequence similarities	Belongs to the macoilin family.

Ontologies

Keywords
Cellular component	Cell projection Membrane Nucleus
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brain development Inferred from electronic annotation. Source: Compara
Cellular_component	axon Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuron projection terminus Inferred from sequence or structural similarity PubMed 15255972. Source: BHF-UCL nuclear membrane Inferred from sequence or structural similarity PubMed 15255972. Source: BHF-UCL synapse Inferred from sequence or structural similarity PubMed 15255972. Source: BHF-UCL synapse part Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N5G2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N5G2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     27-384: Missing.

Isoform 3 (identifier: Q8N5G2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     158-158: C → W
     159-385: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 664	664	Macoilin		PRO_0000070266
Regions
	Transmembrane	28 – 48	21	Helical; Potential
	Transmembrane	75 – 95	21	Helical; Potential
	Transmembrane	120 – 140	21	Helical; Potential
	Transmembrane	154 – 174	21	Helical; Potential
Amino acid modifications
	Modified residue	332	1	Phosphoserine Ref.6 Ref.8 Ref.9
	Modified residue	631	1	Phosphoserine Ref.6
	Modified residue	634	1	Phosphoserine Ref.8
	Glycosylation	324	1	N-linked (GlcNAc...) Potential
	Glycosylation	340	1	N-linked (GlcNAc...) Potential
	Glycosylation	452	1	N-linked (GlcNAc...) Potential
	Glycosylation	655	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	27 – 384	358	Missing in isoform 2.		VSP_017203
	Alternative sequence	158	1	C → W in isoform 3.		VSP_017204
	Alternative sequence	159 – 385	227	Missing in isoform 3.		VSP_017205
Experimental info
	Sequence conflict	577	1	R → I in BAA91786. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: C0F16B66E13AC281	FASTA	664	76,178
	10 20 30 40 50 60 MKRRNADCSK LRRPLKRNRI TEGIYGSTFL YLKFLVVWAL VLLADFVLEF RFEYLWPFWL 70 80 90 100 110 120 FIRSVYDSFR YQGLAFSVFF VCVAFTSNII CLLFIPIQWL FFAASTYVWV QYVWHTERGV 130 140 150 160 170 180 CLPTVSLWIL FVYIEAAIRF KDLKNFHVDL CRPFAAHCIG YPVVTLGFGF KSYVSYKMRL 190 200 210 220 230 240 RKQKEVQKEN EFYMQLLQQA LPPEQQMLQK QEKEAEEAAK GLPDMDSSIL IHHNGGIPAN 250 260 270 280 290 300 KKLSTTLPEI EYREKGKEKD KDAKKHNLGI NNNNILQPVD SKIQEIEYME NHINSKRLNN 310 320 330 340 350 360 DLVGSTENLL KEDSCTASSK NYKNASGVVN SSPRSHSATN GSIPSSSSKN EKKQKCTSKS 370 380 390 400 410 420 PSTHKDLMEN CIPNNQLSKP DALVRLEQDI KKLKADLQAS RQVEQELRSQ ISSLSSTERG 430 440 450 460 470 480 IRSEMGQLRQ ENELLQNKLH NAVQMKQKDK QNISQLEKKL KAEQEARSFV EKQLMEEKKR 490 500 510 520 530 540 KKLEEATAAR AVAFAAASRG ECTETLRNRI RELEAEGKKL TMDMKVKEDQ IRELELKVQE 550 560 570 580 590 600 LRKYKENEKD TEVLMSALSA MQDKTQHLEN SLSAETRIKL DLFSALGDAK RQLEIAQGQI 610 620 630 640 650 660 LQKDQEIKDL KQKIAEVMAV MPSITYSAAT SPLSPVSPHY SSKFVETSPS GLDPNASVYQ PLKK
	Isoform 2 [UniParc]. Checksum: 7E4942B918C8C88A	FASTA	306	35,026
	10 20 30 40 50 60 MKRRNADCSK LRRPLKRNRI TEGIYGRLEQ DIKKLKADLQ ASRQVEQELR SQISSLSSTE 70 80 90 100 110 120 RGIRSEMGQL RQENELLQNK LHNAVQMKQK DKQNISQLEK KLKAEQEARS FVEKQLMEEK 130 140 150 160 170 180 KRKKLEEATA ARAVAFAAAS RGECTETLRN RIRELEAEGK KLTMDMKVKE DQIRELELKV 190 200 210 220 230 240 QELRKYKENE KDTEVLMSAL SAMQDKTQHL ENSLSAETRI KLDLFSALGD AKRQLEIAQG 250 260 270 280 290 300 QILQKDQEIK DLKQKIAEVM AVMPSITYSA ATSPLSPVSP HYSSKFVETS PSGLDPNASV YQPLKK
	Isoform 3 [UniParc]. Checksum: 91381E4E67C52F25	FASTA	437	50,791
	10 20 30 40 50 60 MKRRNADCSK LRRPLKRNRI TEGIYGSTFL YLKFLVVWAL VLLADFVLEF RFEYLWPFWL 70 80 90 100 110 120 FIRSVYDSFR YQGLAFSVFF VCVAFTSNII CLLFIPIQWL FFAASTYVWV QYVWHTERGV 130 140 150 160 170 180 CLPTVSLWIL FVYIEAAIRF KDLKNFHVDL CRPFAAHWLE QDIKKLKADL QASRQVEQEL 190 200 210 220 230 240 RSQISSLSST ERGIRSEMGQ LRQENELLQN KLHNAVQMKQ KDKQNISQLE KKLKAEQEAR 250 260 270 280 290 300 SFVEKQLMEE KKRKKLEEAT AARAVAFAAA SRGECTETLR NRIRELEAEG KKLTMDMKVK 310 320 330 340 350 360 EDQIRELELK VQELRKYKEN EKDTEVLMSA LSAMQDKTQH LENSLSAETR IKLDLFSALG 370 380 390 400 410 420 DAKRQLEIAQ GQILQKDQEI KDLKQKIAEV MAVMPSITYS AATSPLSPVS PHYSSKFVET 430 SPSGLDPNAS VYQPLKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of macoilin as a novel membrane-associated coiled-coil tetraspanin protein." Huang C.-H., Peng J., Ye T., Chen Y. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332 AND SER-631, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332 AND SER-634, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY845015 mRNA. Translation: AAX11913.1. AY845036 mRNA. Translation: AAX11934.1. AY845035 mRNA. Translation: AAX11933.1. AK001609 mRNA. Translation: BAA91786.1. AL031284, BX572623 Genomic DNA. Translation: CAM12861.1. BX572623, AL031284 Genomic DNA. Translation: CAM12862.1. CH471059 Genomic DNA. Translation: EAX07876.1. BC032427 mRNA. Translation: AAH32427.1.
IPI	IPI00296938. IPI00373988. IPI00719801.
RefSeq	NP_060672.2. NM_018202.4.
UniGene	Hs.189782.
3D structure databases
ProteinModelPortal	Q8N5G2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N5G2. 3 interactions.
MINT	MINT-1185124.
STRING	9606.ENSP00000363463.
PTM databases
PhosphoSite	Q8N5G2.
Polymorphism databases
DMDM	74728992.
Proteomic databases
PaxDb	Q8N5G2.
PRIDE	Q8N5G2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000374343; ENSP00000363463; ENSG00000204178. ENST00000399763; ENSP00000382666; ENSG00000204178. ENST00000399766; ENSP00000382668; ENSG00000204178.
GeneID	55219.
KEGG	hsa:55219.
UCSC	uc001bkk.3. human. uc009vru.3. human. uc009vrv.3. human.
Organism-specific databases
CTD	55219.
GeneCards	GC01P025758.
HGNC	HGNC:25572. TMEM57.
HPA	HPA019680.
MIM	610301. gene.
neXtProt	NX_Q8N5G2.
PharmGKB	PA142670773.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG294495.
HOGENOM	HOG000049147.
HOVERGEN	HBG059955.
InParanoid	Q8N5G2.
OMA	VQELHKY.
OrthoDB	EOG4QRH3R.
Gene expression databases
ArrayExpress	Q8N5G2.
Bgee	Q8N5G2.
CleanEx	HS_TMEM57.
Genevestigator	Q8N5G2.
GermOnline	ENSG00000204178. Homo sapiens.
Family and domain databases
InterPro	IPR019130. Macoilin. [Graphical view]
Pfam	PF09726. Macoilin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TMEM57. human.
GenomeRNAi	55219.
NextBio	59192.
SOURCE	Search...

Entry information

Entry name

MACOI_HUMAN

Accession

Primary (citable) accession number: Q8N5G2
Secondary accession number(s): B1AK00 , Q2TLX5, Q2TLX6, Q9NVG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.