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Protein

NF-kappa-B-activating protein

Gene

NKAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T-cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter.2 Publications

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • chromatin DNA binding Source: Ensembl
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Notch signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B-activating protein
Gene namesi
Name:NKAP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:29873. NKAP.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162397584.

Polymorphism and mutation databases

BioMutaiNKAP.
DMDMi74728990.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415NF-kappa-B-activating proteinPRO_0000259645Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei7 – 71PhosphoserineCombined sources
Modified residuei9 – 91PhosphoserineCombined sources
Modified residuei50 – 501PhosphoserineCombined sources
Modified residuei64 – 641PhosphoserineCombined sources
Modified residuei112 – 1121N6-acetyllysineCombined sources
Modified residuei149 – 1491PhosphoserineCombined sources
Modified residuei157 – 1571PhosphoserineCombined sources
Modified residuei161 – 1611PhosphothreonineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8N5F7.
MaxQBiQ8N5F7.
PaxDbiQ8N5F7.
PeptideAtlasiQ8N5F7.
PRIDEiQ8N5F7.

PTM databases

iPTMnetiQ8N5F7.
PhosphoSiteiQ8N5F7.

Expressioni

Gene expression databases

BgeeiENSG00000101882.
CleanExiHS_NKAP.
GenevisibleiQ8N5F7. HS.

Organism-specific databases

HPAiHPA000916.

Interactioni

Subunit structurei

Component of the Notch corepressor complex. Interacts with CIR1 and HDAC3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DDX41Q9UJV92EBI-721539,EBI-1046350
DHX8Q145622EBI-721539,EBI-2511477
PRPF3O433952EBI-721539,EBI-744322
PRPF40AO754002EBI-721539,EBI-473291
RBMX2Q9Y3882EBI-721539,EBI-7704044
U2AF1Q010812EBI-721539,EBI-632461

Protein-protein interaction databases

BioGridi122723. 59 interactions.
IntActiQ8N5F7. 25 interactions.
STRINGi9606.ENSP00000360464.

Structurei

3D structure databases

ProteinModelPortaliQ8N5F7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni179 – 27294Necessary for interaction with CIR1Add
BLAST
Regioni273 – 415143Necessary for interaction with HDAC3 and transcriptional repressionAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi5 – 105101Ser-richAdd
BLAST
Compositional biasi182 – 25978Lys-richAdd
BLAST

Sequence similaritiesi

Belongs to the UPF0396 family.Curated

Phylogenomic databases

eggNOGiKOG2812. Eukaryota.
ENOG4111VW4. LUCA.
GeneTreeiENSGT00510000047021.
HOGENOMiHOG000049099.
HOVERGENiHBG068915.
InParanoidiQ8N5F7.
OMAiWPEYYEK.
OrthoDBiEOG091G0NTO.
PhylomeDBiQ8N5F7.
TreeFamiTF315333.

Family and domain databases

InterProiIPR009269. NKAP/UPF0396.
[Graphical view]
PANTHERiPTHR13087. PTHR13087. 1 hit.
PfamiPF06047. SynMuv_product. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8N5F7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPVSGSRSP DREASGSGGR RRSSSKSPKP SKSARSPRGR RSRSHSCSRS
60 70 80 90 100
GDRNGLTHQL GGLSQGSRNQ SYRSRSRSRS RERPSAPRGI PFASASSSVY
110 120 130 140 150
YGSYSRPYGS DKPWPSLLDK EREESLRQKR LSERERIGEL GAPEVWGLSP
160 170 180 190 200
KNPEPDSDEH TPVEDEEPKK STTSASTSEE EKKKKSSRSK ERSKKRRKKK
210 220 230 240 250
SSKRKHKKYS EDSDSDSDSE TDSSDEDNKR RAKKAKKKEK KKKHRSKKYK
260 270 280 290 300
KKRSKKSRKE SSDSSSKESQ EEFLENPWKD RTKAEEPSDL IGPEAPKTLT
310 320 330 340 350
SQDDKPLNYG HALLPGEGAA MAEYVKAGKR IPRRGEIGLT SEEIASFECS
360 370 380 390 400
GYVMSGSRHR RMEAVRLRKE NQIYSADEKR ALASFNQEER RKRENKILAS
410
FREMVYRKTK GKDDK
Length:415
Mass (Da):47,138
Last modified:October 1, 2002 - v1
Checksum:i44BD1DE20F7ED35A
GO

Sequence cautioni

The sequence AAH71686 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571T → A in BAB15428 (PubMed:14702039).Curated
Sequence conflicti280 – 2801D → Y in AAH15354 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti115 – 1151P → H.
Corresponds to variant rs34728541 [ dbSNP | Ensembl ].
VAR_053799

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY388958 mRNA. Translation: AAQ90402.1.
AK026279 mRNA. Translation: BAB15428.1.
BC015354 mRNA. Translation: AAH15354.1.
BC032442 mRNA. Translation: AAH32442.1.
BC071686 mRNA. Translation: AAH71686.1. Sequence problems.
CCDSiCCDS14592.1.
RefSeqiNP_078804.2. NM_024528.3.
UniGeneiHs.522771.

Genome annotation databases

EnsembliENST00000371410; ENSP00000360464; ENSG00000101882.
GeneIDi79576.
KEGGihsa:79576.
UCSCiuc004esh.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY388958 mRNA. Translation: AAQ90402.1.
AK026279 mRNA. Translation: BAB15428.1.
BC015354 mRNA. Translation: AAH15354.1.
BC032442 mRNA. Translation: AAH32442.1.
BC071686 mRNA. Translation: AAH71686.1. Sequence problems.
CCDSiCCDS14592.1.
RefSeqiNP_078804.2. NM_024528.3.
UniGeneiHs.522771.

3D structure databases

ProteinModelPortaliQ8N5F7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122723. 59 interactions.
IntActiQ8N5F7. 25 interactions.
STRINGi9606.ENSP00000360464.

PTM databases

iPTMnetiQ8N5F7.
PhosphoSiteiQ8N5F7.

Polymorphism and mutation databases

BioMutaiNKAP.
DMDMi74728990.

Proteomic databases

EPDiQ8N5F7.
MaxQBiQ8N5F7.
PaxDbiQ8N5F7.
PeptideAtlasiQ8N5F7.
PRIDEiQ8N5F7.

Protocols and materials databases

DNASUi79576.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371410; ENSP00000360464; ENSG00000101882.
GeneIDi79576.
KEGGihsa:79576.
UCSCiuc004esh.4. human.

Organism-specific databases

CTDi79576.
GeneCardsiNKAP.
HGNCiHGNC:29873. NKAP.
HPAiHPA000916.
MIMi300766. gene.
neXtProtiNX_Q8N5F7.
PharmGKBiPA162397584.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2812. Eukaryota.
ENOG4111VW4. LUCA.
GeneTreeiENSGT00510000047021.
HOGENOMiHOG000049099.
HOVERGENiHBG068915.
InParanoidiQ8N5F7.
OMAiWPEYYEK.
OrthoDBiEOG091G0NTO.
PhylomeDBiQ8N5F7.
TreeFamiTF315333.

Miscellaneous databases

GeneWikiiNKAP.
GenomeRNAii79576.
PROiQ8N5F7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101882.
CleanExiHS_NKAP.
GenevisibleiQ8N5F7. HS.

Family and domain databases

InterProiIPR009269. NKAP/UPF0396.
[Graphical view]
PANTHERiPTHR13087. PTHR13087. 1 hit.
PfamiPF06047. SynMuv_product. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNKAP_HUMAN
AccessioniPrimary (citable) accession number: Q8N5F7
Secondary accession number(s): Q6IPW6, Q96BQ2, Q9H638
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families
  6. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.