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Q8N568 (DCLK2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase DCLK2

EC=2.7.11.1
Alternative name(s):
CaMK-like CREB regulatory kinase 2
Short name=CL2
Short name=CLICK-II
Short name=CLICK2
Doublecortin domain-containing protein 3B
Doublecortin-like and CAM kinase-like 2
Doublecortin-like kinase 2
Gene names
Name:DCLK2
Synonyms:DCAMKL2, DCDC3B, DCK2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length766 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Binds to and stabilizes microtubules By similarity. Interacts with MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK9/JNK2, PPP1R9B/NEURABIN-2 and actin By similarity.

Subcellular location

Cytoplasmcytoskeleton. Note: Colocalizes with microtubules By similarity.

Tissue specificity

Expressed in the brain, heart and eyes. Ref.5

Developmental stage

Expressed in fetal brain, and, to a lower extent, in fetal kidney. Ref.5

Domain

The doublecortin domains are involved in the colocalization with microtubules By similarity.

Post-translational modification

Autophosphorylated By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Contains 2 doublecortin domains.

Contains 1 protein kinase domain.

Sequence caution

The sequence BAD92418.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processintracellular signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

cytoskeleton

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8N568-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8N568-2)

The sequence of this isoform differs from the canonical sequence as follows:
     353-353: Missing.
Note: By homology to mouse isoform 2.
Isoform 3 (identifier: Q8N568-3)

The sequence of this isoform differs from the canonical sequence as follows:
     321-321: V → VKRGGHYSSAYSTAKSPV

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 766766Serine/threonine-protein kinase DCLK2
PRO_0000085922

Regions

Domain72 – 15887Doublecortin 1
Domain197 – 28084Doublecortin 2
Domain394 – 651258Protein kinase
Nucleotide binding400 – 4089ATP By similarity
Compositional bias293 – 36270Ser-rich
Compositional bias713 – 75341Pro-rich

Sites

Active site5151Proton acceptor By similarity
Binding site4231ATP By similarity

Amino acid modifications

Modified residue6471Phosphoserine By similarity
Modified residue6661Phosphothreonine By similarity

Natural variations

Alternative sequence3211V → VKRGGHYSSAYSTAKSPV in isoform 3.
VSP_012793
Alternative sequence3531Missing in isoform 2.
VSP_012794
Natural variant1191G → C. Ref.7
Corresponds to variant rs56327537 [ dbSNP | Ensembl ].
VAR_040441
Natural variant3721R → H. Ref.7
Corresponds to variant rs34386880 [ dbSNP | Ensembl ].
VAR_040442
Natural variant5831I → V. Ref.7
Corresponds to variant rs35745104 [ dbSNP | Ensembl ].
VAR_040443

Experimental info

Sequence conflict6941A → V in CAD39156. Ref.3
Sequence conflict7481P → C in BAD92418. Ref.1
Sequence conflict7481P → C in CAD39156. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 4.
Checksum: 024F3223874AE83C

FASTA76683,606
        10         20         30         40         50         60 
MASTRSIELE HFEERDKRPR PGSRRGAPSS SGGSSSSGPK GNGLIPSPAH SAHCSFYRTR 

        70         80         90        100        110        120 
TLQALSSEKK AKKARFYRNG DRYFKGLVFA ISSDRFRSFD ALLIELTRSL SDNVNLPQGV 

       130        140        150        160        170        180 
RTIYTIDGSR KVTSLDELLE GESYVCASNE PFRKVDYTKN INPNWSVNIK GGTSRALAAA 

       190        200        210        220        230        240 
SSVKSEVKES KDFIKPKLVT VIRSGVKPRK AVRILLNKKT AHSFEQVLTD ITEAIKLDSG 

       250        260        270        280        290        300 
VVKRLCTLDG KQVTCLQDFF GDDDVFIACG PEKFRYAQDD FVLDHSECRV LKSSYSRSSA 

       310        320        330        340        350        360 
VKYSGSKSPG PSRRSKSPAS VNGTPSSQLS TPKSTKSSSS SPTSPGSFRG LKQISAHGRS 

       370        380        390        400        410        420 
SSNVNGGPEL DRCISPEGVN GNRCSESSTL LEKYKIGKVI GDGNFAVVKE CIDRSTGKEF 

       430        440        450        460        470        480 
ALKIIDKAKC CGKEHLIENE VSILRRVKHP NIIMLVEEME TATELFLVME LVKGGDLFDA 

       490        500        510        520        530        540 
ITSSTKYTER DGSAMVYNLA NALRYLHGLS IVHRDIKPEN LLVCEYPDGT KSLKLGDFGL 

       550        560        570        580        590        600 
ATVVEGPLYT VCGTPTYVAP EIIAETGYGL KVDIWAAGVI TYILLCGFPP FRSENNLQED 

       610        620        630        640        650        660 
LFDQILAGKL EFPAPYWDNI TDSAKELISQ MLQVNVEARC TAGQILSHPW VSDDASQENN 

       670        680        690        700        710        720 
MQAEVTGKLK QHFNNALPKQ NSTTTGVSVI MNTALDKEGQ IFCSKHCQDS GRPGMEPISP 

       730        740        750        760 
VPPSVEEIPV PGEAVPAPTP PESPTPHPPP AAPGGERAGT WRRHRD 

« Hide

Isoform 2 [UniParc].

Checksum: 48EE55037EFF8AD8
Show »

FASTA76583,478
Isoform 3 [UniParc].

Checksum: D69154961F0CFF77
Show »

FASTA78385,384

References

« Hide 'large scale' references
[1]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 68-766 (ISOFORM 3).
Tissue: Amygdala.
[4]"The evolving doublecortin (DCX) superfamily."
Reiner O., Coquelle F.M., Peter B., Levy T., Kaplan A., Sapir T., Orr I., Barkai N., Eichele G., Bergmann S.
BMC Genomics 7:188-188(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[5]"Alternative transcripts of Dclk1 and Dclk2 and their expression in doublecortin knockout mice."
Tuy F.P.D., Saillour Y., Kappeler C., Chelly J., Francis F.
Dev. Neurosci. 30:171-186(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[6]"The doublecortin gene family and disorders of neuronal structure."
Dijkmans T.F., van Hooijdonk L.W.A., Fitzsimons C.P., Vreugdenhil E.
Cent. Nerv. Syst. Agents Med. Chem. 10:32-46(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW, GENE FAMILY.
[7]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] CYS-119; HIS-372 AND VAL-583.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB209181 mRNA. Translation: BAD92418.1. Different initiation.
AC093748 Genomic DNA. No translation available.
AC105343 Genomic DNA. No translation available.
AC108934 Genomic DNA. No translation available.
AC118064 Genomic DNA. No translation available.
AL834498 mRNA. Translation: CAD39156.1.
RefSeqNP_001035350.2. NM_001040260.3.
NP_001035351.4. NM_001040261.4.
XP_005262843.1. XM_005262786.1.
UniGeneHs.591683.

3D structure databases

ProteinModelPortalQ8N568.
SMRQ8N568. Positions 64-169, 352-694.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127929. 2 interactions.
IntActQ8N568. 2 interactions.
STRING9606.ENSP00000296550.

Chemistry

ChEMBLCHEMBL5519.
GuidetoPHARMACOLOGY2006.

PTM databases

PhosphoSiteQ8N568.

Polymorphism databases

DMDM296439470.

Proteomic databases

PaxDbQ8N568.
PRIDEQ8N568.

Protocols and materials databases

DNASU166614.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000296550; ENSP00000296550; ENSG00000170390. [Q8N568-1]
ENST00000302176; ENSP00000303887; ENSG00000170390. [Q8N568-3]
ENST00000506325; ENSP00000427235; ENSG00000170390. [Q8N568-2]
GeneID166614.
KEGGhsa:166614.
UCSCuc003ilm.4. human. [Q8N568-1]
uc003ilo.4. human. [Q8N568-3]

Organism-specific databases

CTD166614.
GeneCardsGC04P150999.
HGNCHGNC:19002. DCLK2.
HPAHPA015770.
MIM613166. gene.
neXtProtNX_Q8N568.
PharmGKBPA162383366.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000230855.
HOVERGENHBG003790.
KOK08805.
OMATWRRHRD.
OrthoDBEOG7M98FQ.
PhylomeDBQ8N568.
TreeFamTF318770.

Enzyme and pathway databases

SignaLinkQ8N568.

Gene expression databases

ArrayExpressQ8N568.
BgeeQ8N568.
CleanExHS_DCLK2.
GenevestigatorQ8N568.

Family and domain databases

Gene3D3.10.20.230. 2 hits.
InterProIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR003533. Doublecortin_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR24347. PTHR24347. 1 hit.
PfamPF03607. DCX. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00537. DCX. 2 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50309. DC. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi166614.
NextBio88597.
PROQ8N568.
SOURCESearch...

Entry information

Entry nameDCLK2_HUMAN
AccessionPrimary (citable) accession number: Q8N568
Secondary accession number(s): C9J5Q9, Q59GC8, Q8N399
Entry history
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: May 18, 2010
Last modified: April 16, 2014
This is version 109 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM