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Protein

Serine/threonine-protein kinase DCLK2

Gene

DCLK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei423ATPPROSITE-ProRule annotation1
Active sitei515Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi400 – 408ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS10121-MONOMER.
SignaLinkiQ8N568.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase DCLK2 (EC:2.7.11.1)
Alternative name(s):
CaMK-like CREB regulatory kinase 2
Short name:
CL2
Short name:
CLICK-II
Short name:
CLICK2
Doublecortin domain-containing protein 3B
Doublecortin-like and CAM kinase-like 2
Doublecortin-like kinase 2
Gene namesi
Name:DCLK2
Synonyms:DCAMKL2, DCDC3B, DCK2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:19002. DCLK2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000170390.
PharmGKBiPA162383366.

Chemistry databases

ChEMBLiCHEMBL5519.
GuidetoPHARMACOLOGYi2006.

Polymorphism and mutation databases

BioMutaiDCLK2.
DMDMi296439470.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859221 – 766Serine/threonine-protein kinase DCLK2Add BLAST766

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61PhosphothreonineCombined sources1
Modified residuei362PhosphoserineCombined sources1
Modified residuei647PhosphoserineBy similarity1
Modified residuei666PhosphothreonineBy similarity1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8N568.
PaxDbiQ8N568.
PeptideAtlasiQ8N568.
PRIDEiQ8N568.

PTM databases

iPTMnetiQ8N568.
PhosphoSitePlusiQ8N568.

Expressioni

Tissue specificityi

Expressed in the brain, heart and eyes.1 Publication

Developmental stagei

Expressed in fetal brain, and, to a lower extent, in fetal kidney.1 Publication

Gene expression databases

BgeeiENSG00000170390.
CleanExiHS_DCLK2.
ExpressionAtlasiQ8N568. baseline and differential.
GenevisibleiQ8N568. HS.

Organism-specific databases

HPAiHPA015770.

Interactioni

Subunit structurei

Binds to and stabilizes microtubules. Interacts with MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK9/JNK2, PPP1R9B/NEURABIN-2 and actin.By similarity

Protein-protein interaction databases

BioGridi127929. 5 interactors.
IntActiQ8N568. 4 interactors.
STRINGi9606.ENSP00000303887.

Chemistry databases

BindingDBiQ8N568.

Structurei

3D structure databases

ProteinModelPortaliQ8N568.
SMRiQ8N568.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 158Doublecortin 1PROSITE-ProRule annotationAdd BLAST87
Domaini197 – 280Doublecortin 2PROSITE-ProRule annotationAdd BLAST84
Domaini394 – 651Protein kinasePROSITE-ProRule annotationAdd BLAST258

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi293 – 362Ser-richAdd BLAST70
Compositional biasi713 – 753Pro-richAdd BLAST41

Domaini

The doublecortin domains are involved in the colocalization with microtubules.By similarity

Sequence similaritiesi

Contains 2 doublecortin domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0615. Eukaryota.
ENOG410YA63. LUCA.
GeneTreeiENSGT00840000129744.
HOGENOMiHOG000230855.
HOVERGENiHBG003790.
InParanoidiQ8N568.
KOiK08805.
OMAiECIDRST.
OrthoDBiEOG091G02RF.
PhylomeDBiQ8N568.
TreeFamiTF318770.

Family and domain databases

CDDicd01617. DCX. 2 hits.
Gene3Di3.10.20.230. 2 hits.
InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR003533. Doublecortin_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 2 hits.
PfamiPF03607. DCX. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00537. DCX. 2 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50309. DC. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N568-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTRSIELE HFEERDKRPR PGSRRGAPSS SGGSSSSGPK GNGLIPSPAH
60 70 80 90 100
SAHCSFYRTR TLQALSSEKK AKKARFYRNG DRYFKGLVFA ISSDRFRSFD
110 120 130 140 150
ALLIELTRSL SDNVNLPQGV RTIYTIDGSR KVTSLDELLE GESYVCASNE
160 170 180 190 200
PFRKVDYTKN INPNWSVNIK GGTSRALAAA SSVKSEVKES KDFIKPKLVT
210 220 230 240 250
VIRSGVKPRK AVRILLNKKT AHSFEQVLTD ITEAIKLDSG VVKRLCTLDG
260 270 280 290 300
KQVTCLQDFF GDDDVFIACG PEKFRYAQDD FVLDHSECRV LKSSYSRSSA
310 320 330 340 350
VKYSGSKSPG PSRRSKSPAS VNGTPSSQLS TPKSTKSSSS SPTSPGSFRG
360 370 380 390 400
LKQISAHGRS SSNVNGGPEL DRCISPEGVN GNRCSESSTL LEKYKIGKVI
410 420 430 440 450
GDGNFAVVKE CIDRSTGKEF ALKIIDKAKC CGKEHLIENE VSILRRVKHP
460 470 480 490 500
NIIMLVEEME TATELFLVME LVKGGDLFDA ITSSTKYTER DGSAMVYNLA
510 520 530 540 550
NALRYLHGLS IVHRDIKPEN LLVCEYPDGT KSLKLGDFGL ATVVEGPLYT
560 570 580 590 600
VCGTPTYVAP EIIAETGYGL KVDIWAAGVI TYILLCGFPP FRSENNLQED
610 620 630 640 650
LFDQILAGKL EFPAPYWDNI TDSAKELISQ MLQVNVEARC TAGQILSHPW
660 670 680 690 700
VSDDASQENN MQAEVTGKLK QHFNNALPKQ NSTTTGVSVI MNTALDKEGQ
710 720 730 740 750
IFCSKHCQDS GRPGMEPISP VPPSVEEIPV PGEAVPAPTP PESPTPHPPP
760
AAPGGERAGT WRRHRD
Length:766
Mass (Da):83,606
Last modified:May 18, 2010 - v4
Checksum:i024F3223874AE83C
GO
Isoform 2 (identifier: Q8N568-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     353-353: Missing.

Note: By homology to mouse isoform 2.
Show »
Length:765
Mass (Da):83,478
Checksum:i48EE55037EFF8AD8
GO
Isoform 3 (identifier: Q8N568-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     321-321: V → VKRGGHYSSAYSTAKSPV

Show »
Length:783
Mass (Da):85,384
Checksum:iD69154961F0CFF77
GO

Sequence cautioni

The sequence BAD92418 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti694A → V in CAD39156 (PubMed:17974005).Curated1
Sequence conflicti748P → C in BAD92418 (Ref. 1) Curated1
Sequence conflicti748P → C in CAD39156 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_040441119G → C.1 PublicationCorresponds to variant rs56327537dbSNPEnsembl.1
Natural variantiVAR_073158212V → M Found in a patient with hereditary motor and sensory neuropathy; unknown pathological significance. 1 PublicationCorresponds to variant rs759398144dbSNPEnsembl.1
Natural variantiVAR_040442372R → H.1 PublicationCorresponds to variant rs34386880dbSNPEnsembl.1
Natural variantiVAR_040443583I → V.1 PublicationCorresponds to variant rs35745104dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012793321V → VKRGGHYSSAYSTAKSPV in isoform 3. 1 Publication1
Alternative sequenceiVSP_012794353Missing in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB209181 mRNA. Translation: BAD92418.1. Different initiation.
AC093748 Genomic DNA. No translation available.
AC105343 Genomic DNA. No translation available.
AC108934 Genomic DNA. No translation available.
AC118064 Genomic DNA. No translation available.
AL834498 mRNA. Translation: CAD39156.1.
CCDSiCCDS34076.1. [Q8N568-1]
CCDS47142.2. [Q8N568-3]
RefSeqiNP_001035350.2. NM_001040260.3. [Q8N568-1]
NP_001035351.4. NM_001040261.4. [Q8N568-3]
XP_005262843.1. XM_005262786.2. [Q8N568-2]
UniGeneiHs.591683.

Genome annotation databases

EnsembliENST00000296550; ENSP00000296550; ENSG00000170390. [Q8N568-1]
ENST00000302176; ENSP00000303887; ENSG00000170390. [Q8N568-3]
ENST00000506325; ENSP00000427235; ENSG00000170390. [Q8N568-2]
GeneIDi166614.
KEGGihsa:166614.
UCSCiuc003ilm.5. human. [Q8N568-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB209181 mRNA. Translation: BAD92418.1. Different initiation.
AC093748 Genomic DNA. No translation available.
AC105343 Genomic DNA. No translation available.
AC108934 Genomic DNA. No translation available.
AC118064 Genomic DNA. No translation available.
AL834498 mRNA. Translation: CAD39156.1.
CCDSiCCDS34076.1. [Q8N568-1]
CCDS47142.2. [Q8N568-3]
RefSeqiNP_001035350.2. NM_001040260.3. [Q8N568-1]
NP_001035351.4. NM_001040261.4. [Q8N568-3]
XP_005262843.1. XM_005262786.2. [Q8N568-2]
UniGeneiHs.591683.

3D structure databases

ProteinModelPortaliQ8N568.
SMRiQ8N568.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127929. 5 interactors.
IntActiQ8N568. 4 interactors.
STRINGi9606.ENSP00000303887.

Chemistry databases

BindingDBiQ8N568.
ChEMBLiCHEMBL5519.
GuidetoPHARMACOLOGYi2006.

PTM databases

iPTMnetiQ8N568.
PhosphoSitePlusiQ8N568.

Polymorphism and mutation databases

BioMutaiDCLK2.
DMDMi296439470.

Proteomic databases

MaxQBiQ8N568.
PaxDbiQ8N568.
PeptideAtlasiQ8N568.
PRIDEiQ8N568.

Protocols and materials databases

DNASUi166614.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296550; ENSP00000296550; ENSG00000170390. [Q8N568-1]
ENST00000302176; ENSP00000303887; ENSG00000170390. [Q8N568-3]
ENST00000506325; ENSP00000427235; ENSG00000170390. [Q8N568-2]
GeneIDi166614.
KEGGihsa:166614.
UCSCiuc003ilm.5. human. [Q8N568-1]

Organism-specific databases

CTDi166614.
GeneCardsiDCLK2.
HGNCiHGNC:19002. DCLK2.
HPAiHPA015770.
MIMi613166. gene.
neXtProtiNX_Q8N568.
OpenTargetsiENSG00000170390.
PharmGKBiPA162383366.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0615. Eukaryota.
ENOG410YA63. LUCA.
GeneTreeiENSGT00840000129744.
HOGENOMiHOG000230855.
HOVERGENiHBG003790.
InParanoidiQ8N568.
KOiK08805.
OMAiECIDRST.
OrthoDBiEOG091G02RF.
PhylomeDBiQ8N568.
TreeFamiTF318770.

Enzyme and pathway databases

BioCyciZFISH:HS10121-MONOMER.
SignaLinkiQ8N568.

Miscellaneous databases

GenomeRNAii166614.
PROiQ8N568.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170390.
CleanExiHS_DCLK2.
ExpressionAtlasiQ8N568. baseline and differential.
GenevisibleiQ8N568. HS.

Family and domain databases

CDDicd01617. DCX. 2 hits.
Gene3Di3.10.20.230. 2 hits.
InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR003533. Doublecortin_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 2 hits.
PfamiPF03607. DCX. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00537. DCX. 2 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50309. DC. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCLK2_HUMAN
AccessioniPrimary (citable) accession number: Q8N568
Secondary accession number(s): C9J5Q9, Q59GC8, Q8N399
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: May 18, 2010
Last modified: November 2, 2016
This is version 133 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.