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Q8N568

- DCLK2_HUMAN

UniProt

Q8N568 - DCLK2_HUMAN

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Protein

Serine/threonine-protein kinase DCLK2

Gene
DCLK2, DCAMKL2, DCDC3B, DCK2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei423 – 4231ATP By similarity
Active sitei515 – 5151Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi400 – 4089ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ8N568.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase DCLK2 (EC:2.7.11.1)
Alternative name(s):
CaMK-like CREB regulatory kinase 2
Short name:
CL2
Short name:
CLICK-II
Short name:
CLICK2
Doublecortin domain-containing protein 3B
Doublecortin-like and CAM kinase-like 2
Doublecortin-like kinase 2
Gene namesi
Name:DCLK2
Synonyms:DCAMKL2, DCDC3B, DCK2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:19002. DCLK2.

Subcellular locationi

Cytoplasmcytoskeleton
Note: Colocalizes with microtubules By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. cytoskeleton Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162383366.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 766766Serine/threonine-protein kinase DCLK2PRO_0000085922Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei647 – 6471Phosphoserine By similarity
Modified residuei666 – 6661Phosphothreonine By similarity

Post-translational modificationi

Autophosphorylated By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8N568.
PaxDbiQ8N568.
PRIDEiQ8N568.

PTM databases

PhosphoSiteiQ8N568.

Expressioni

Tissue specificityi

Expressed in the brain, heart and eyes.1 Publication

Developmental stagei

Expressed in fetal brain, and, to a lower extent, in fetal kidney.1 Publication

Gene expression databases

ArrayExpressiQ8N568.
BgeeiQ8N568.
CleanExiHS_DCLK2.
GenevestigatoriQ8N568.

Organism-specific databases

HPAiHPA015770.

Interactioni

Subunit structurei

Binds to and stabilizes microtubules By similarity. Interacts with MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK9/JNK2, PPP1R9B/NEURABIN-2 and actin By similarity.

Protein-protein interaction databases

BioGridi127929. 2 interactions.
IntActiQ8N568. 2 interactions.
STRINGi9606.ENSP00000296550.

Structurei

3D structure databases

ProteinModelPortaliQ8N568.
SMRiQ8N568. Positions 64-169, 352-694.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 15887Doublecortin 1Add
BLAST
Domaini197 – 28084Doublecortin 2Add
BLAST
Domaini394 – 651258Protein kinaseAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi293 – 36270Ser-richAdd
BLAST
Compositional biasi713 – 75341Pro-richAdd
BLAST

Domaini

The doublecortin domains are involved in the colocalization with microtubules By similarity.

Sequence similaritiesi

Contains 2 doublecortin domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000230855.
HOVERGENiHBG003790.
KOiK08805.
OMAiTWRRHRD.
OrthoDBiEOG7M98FQ.
PhylomeDBiQ8N568.
TreeFamiTF318770.

Family and domain databases

Gene3Di3.10.20.230. 2 hits.
InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR003533. Doublecortin_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF03607. DCX. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00537. DCX. 2 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50309. DC. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8N568-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MASTRSIELE HFEERDKRPR PGSRRGAPSS SGGSSSSGPK GNGLIPSPAH    50
SAHCSFYRTR TLQALSSEKK AKKARFYRNG DRYFKGLVFA ISSDRFRSFD 100
ALLIELTRSL SDNVNLPQGV RTIYTIDGSR KVTSLDELLE GESYVCASNE 150
PFRKVDYTKN INPNWSVNIK GGTSRALAAA SSVKSEVKES KDFIKPKLVT 200
VIRSGVKPRK AVRILLNKKT AHSFEQVLTD ITEAIKLDSG VVKRLCTLDG 250
KQVTCLQDFF GDDDVFIACG PEKFRYAQDD FVLDHSECRV LKSSYSRSSA 300
VKYSGSKSPG PSRRSKSPAS VNGTPSSQLS TPKSTKSSSS SPTSPGSFRG 350
LKQISAHGRS SSNVNGGPEL DRCISPEGVN GNRCSESSTL LEKYKIGKVI 400
GDGNFAVVKE CIDRSTGKEF ALKIIDKAKC CGKEHLIENE VSILRRVKHP 450
NIIMLVEEME TATELFLVME LVKGGDLFDA ITSSTKYTER DGSAMVYNLA 500
NALRYLHGLS IVHRDIKPEN LLVCEYPDGT KSLKLGDFGL ATVVEGPLYT 550
VCGTPTYVAP EIIAETGYGL KVDIWAAGVI TYILLCGFPP FRSENNLQED 600
LFDQILAGKL EFPAPYWDNI TDSAKELISQ MLQVNVEARC TAGQILSHPW 650
VSDDASQENN MQAEVTGKLK QHFNNALPKQ NSTTTGVSVI MNTALDKEGQ 700
IFCSKHCQDS GRPGMEPISP VPPSVEEIPV PGEAVPAPTP PESPTPHPPP 750
AAPGGERAGT WRRHRD 766
Length:766
Mass (Da):83,606
Last modified:May 18, 2010 - v4
Checksum:i024F3223874AE83C
GO
Isoform 2 (identifier: Q8N568-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     353-353: Missing.

Note: By homology to mouse isoform 2.

Show »
Length:765
Mass (Da):83,478
Checksum:i48EE55037EFF8AD8
GO
Isoform 3 (identifier: Q8N568-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     321-321: V → VKRGGHYSSAYSTAKSPV

Show »
Length:783
Mass (Da):85,384
Checksum:iD69154961F0CFF77
GO

Sequence cautioni

The sequence BAD92418.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti119 – 1191G → C.1 Publication
Corresponds to variant rs56327537 [ dbSNP | Ensembl ].
VAR_040441
Natural varianti372 – 3721R → H.1 Publication
Corresponds to variant rs34386880 [ dbSNP | Ensembl ].
VAR_040442
Natural varianti583 – 5831I → V.1 Publication
Corresponds to variant rs35745104 [ dbSNP | Ensembl ].
VAR_040443

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei321 – 3211V → VKRGGHYSSAYSTAKSPV in isoform 3. VSP_012793
Alternative sequencei353 – 3531Missing in isoform 2. VSP_012794

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti694 – 6941A → V in CAD39156. 1 Publication
Sequence conflicti748 – 7481P → C in BAD92418. 1 Publication
Sequence conflicti748 – 7481P → C in CAD39156. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB209181 mRNA. Translation: BAD92418.1. Different initiation.
AC093748 Genomic DNA. No translation available.
AC105343 Genomic DNA. No translation available.
AC108934 Genomic DNA. No translation available.
AC118064 Genomic DNA. No translation available.
AL834498 mRNA. Translation: CAD39156.1.
CCDSiCCDS34076.1. [Q8N568-1]
CCDS47142.2. [Q8N568-3]
RefSeqiNP_001035350.2. NM_001040260.3. [Q8N568-1]
NP_001035351.4. NM_001040261.4. [Q8N568-3]
XP_005262843.1. XM_005262786.1. [Q8N568-2]
UniGeneiHs.591683.

Genome annotation databases

EnsembliENST00000296550; ENSP00000296550; ENSG00000170390. [Q8N568-1]
ENST00000302176; ENSP00000303887; ENSG00000170390. [Q8N568-3]
ENST00000506325; ENSP00000427235; ENSG00000170390. [Q8N568-2]
GeneIDi166614.
KEGGihsa:166614.
UCSCiuc003ilm.4. human. [Q8N568-1]
uc003ilo.4. human. [Q8N568-3]

Polymorphism databases

DMDMi296439470.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB209181 mRNA. Translation: BAD92418.1 . Different initiation.
AC093748 Genomic DNA. No translation available.
AC105343 Genomic DNA. No translation available.
AC108934 Genomic DNA. No translation available.
AC118064 Genomic DNA. No translation available.
AL834498 mRNA. Translation: CAD39156.1 .
CCDSi CCDS34076.1. [Q8N568-1 ]
CCDS47142.2. [Q8N568-3 ]
RefSeqi NP_001035350.2. NM_001040260.3. [Q8N568-1 ]
NP_001035351.4. NM_001040261.4. [Q8N568-3 ]
XP_005262843.1. XM_005262786.1. [Q8N568-2 ]
UniGenei Hs.591683.

3D structure databases

ProteinModelPortali Q8N568.
SMRi Q8N568. Positions 64-169, 352-694.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 127929. 2 interactions.
IntActi Q8N568. 2 interactions.
STRINGi 9606.ENSP00000296550.

Chemistry

ChEMBLi CHEMBL5519.
GuidetoPHARMACOLOGYi 2006.

PTM databases

PhosphoSitei Q8N568.

Polymorphism databases

DMDMi 296439470.

Proteomic databases

MaxQBi Q8N568.
PaxDbi Q8N568.
PRIDEi Q8N568.

Protocols and materials databases

DNASUi 166614.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000296550 ; ENSP00000296550 ; ENSG00000170390 . [Q8N568-1 ]
ENST00000302176 ; ENSP00000303887 ; ENSG00000170390 . [Q8N568-3 ]
ENST00000506325 ; ENSP00000427235 ; ENSG00000170390 . [Q8N568-2 ]
GeneIDi 166614.
KEGGi hsa:166614.
UCSCi uc003ilm.4. human. [Q8N568-1 ]
uc003ilo.4. human. [Q8N568-3 ]

Organism-specific databases

CTDi 166614.
GeneCardsi GC04P150999.
HGNCi HGNC:19002. DCLK2.
HPAi HPA015770.
MIMi 613166. gene.
neXtProti NX_Q8N568.
PharmGKBi PA162383366.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000230855.
HOVERGENi HBG003790.
KOi K08805.
OMAi TWRRHRD.
OrthoDBi EOG7M98FQ.
PhylomeDBi Q8N568.
TreeFami TF318770.

Enzyme and pathway databases

SignaLinki Q8N568.

Miscellaneous databases

GenomeRNAii 166614.
NextBioi 88597.
PROi Q8N568.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8N568.
Bgeei Q8N568.
CleanExi HS_DCLK2.
Genevestigatori Q8N568.

Family and domain databases

Gene3Di 3.10.20.230. 2 hits.
InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR003533. Doublecortin_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24347. PTHR24347. 1 hit.
Pfami PF03607. DCX. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00537. DCX. 2 hits.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50309. DC. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 68-766 (ISOFORM 3).
    Tissue: Amygdala.
  4. Cited for: GENE FAMILY.
  5. "Alternative transcripts of Dclk1 and Dclk2 and their expression in doublecortin knockout mice."
    Tuy F.P.D., Saillour Y., Kappeler C., Chelly J., Francis F.
    Dev. Neurosci. 30:171-186(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  6. Cited for: REVIEW, GENE FAMILY.
  7. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] CYS-119; HIS-372 AND VAL-583.

Entry informationi

Entry nameiDCLK2_HUMAN
AccessioniPrimary (citable) accession number: Q8N568
Secondary accession number(s): C9J5Q9, Q59GC8, Q8N399
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 111 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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