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Q8N543

- OGFD1_HUMAN

UniProt

Q8N543 - OGFD1_HUMAN

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Protein

Prolyl 3-hydroxylase OGFOD1

Gene

OGFOD1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Prolyl 3-hydroxylase that catalyzes 3-hydroxylation of 'Pro-62' of small ribosomal subunit RPS23, thereby regulating protein translation termination efficiency. Involved in stress granule formation.3 Publications

Catalytic activityi

Peptidyl L-proline + 2-oxoglutarate + O2 = peptidyl trans-3-hydroxy-L-proline + succinate + CO2.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Fe2+PROSITE-ProRule annotationNote: Binds 1 Fe(2+) ion per subunit.PROSITE-ProRule annotation
  • L-ascorbateBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi155 – 1551IronCurated
Metal bindingi157 – 1571IronCurated
Metal bindingi218 – 2181IronPROSITE-ProRule annotation
Binding sitei228 – 22812-oxoglutaratePROSITE-ProRule annotation

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. L-ascorbic acid binding Source: UniProtKB-KW
  3. peptidyl-proline 3-dioxygenase activity Source: UniProtKB
  4. peptidyl-proline dioxygenase activity Source: UniProtKB

GO - Biological processi

  1. cell proliferation Source: UniProtKB
  2. peptidyl-proline hydroxylation Source: GOC
  3. protein hydroxylation Source: UniProtKB
  4. regulation of translational termination Source: UniProtKB
  5. stress granule assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 3-hydroxylase OGFOD1 (EC:1.14.11.-)
Alternative name(s):
2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1
Termination and polyadenylation 1 homolog
Gene namesi
Name:OGFOD1
Synonyms:KIAA1612, TPA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:25585. OGFOD1.

Subcellular locationi

Isoform 1 : Cytoplasm. Nucleus
Note: Mainly nuclear. A portion relocalizes to cytoplasmic stress granules upon stress.

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. cytoplasmic stress granule Source: UniProtKB
  3. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi155 – 1551H → A: Loss of function. 1 Publication
Mutagenesisi157 – 1571D → A: Loss of function. 1 Publication

Organism-specific databases

PharmGKBiPA143485568.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 542542Prolyl 3-hydroxylase OGFOD1PRO_0000288974Add
BLAST

Proteomic databases

MaxQBiQ8N543.
PaxDbiQ8N543.
PRIDEiQ8N543.

PTM databases

PhosphoSiteiQ8N543.

Expressioni

Gene expression databases

BgeeiQ8N543.
CleanExiHS_OGFOD1.
ExpressionAtlasiQ8N543. baseline and differential.
GenevestigatoriQ8N543.

Organism-specific databases

HPAiHPA003215.

Interactioni

Protein-protein interaction databases

BioGridi120532. 34 interactions.
STRINGi9606.ENSP00000337196.

Structurei

3D structure databases

ProteinModelPortaliQ8N543.
SMRiQ8N543. Positions 72-320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini134 – 239106Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi4 – 2017Nuclear localization signalCuratedAdd
BLAST

Sequence similaritiesi

Belongs to the TPA1 family.Curated
Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG3751.
GeneTreeiENSGT00390000002349.
HOGENOMiHOG000007015.
HOVERGENiHBG056995.
InParanoidiQ8N543.
OMAiFSFVYYE.
OrthoDBiEOG7FBRHW.
PhylomeDBiQ8N543.
TreeFamiTF105920.

Family and domain databases

InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR019601. Oxoglutarate/Fe-dep_Oase_C.
IPR006620. Pro_4_hyd_alph.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
PF10637. Ofd1_CTDD. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8N543-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNGKRPAEPG PARVGKKGKK EVMAEFSDAV TEETLKKQVA EAWSRRTPFS
60 70 80 90 100
HEVIVMDMDP FLHCVIPNFI QSQDFLEGLQ KELMNLDFHE KYNDLYKFQQ
110 120 130 140 150
SDDLKKRREP HISTLRKILF EDFRSWLSDI SKIDLESTID MSCAKYEFTD
160 170 180 190 200
ALLCHDDELE GRRIAFILYL VPPWDRSMGG TLDLYSIDEH FQPKQIVKSL
210 220 230 240 250
IPSWNKLVFF EVSPVSFHQV SEVLSEEKSR LSISGWFHGP SLTRPPNYFE
260 270 280 290 300
PPIPRSPHIP QDHEILYDWI NPTYLDMDYQ VQIQEEFEES SEILLKEFLK
310 320 330 340 350
PEKFTKVCEA LEHGHVEWSS RGPPNKRFYE KAEESKLPEI LKECMKLFRS
360 370 380 390 400
EALFLLLSNF TGLKLHFLAP SEEDEMNDKK EAETTDITEE GTSHSPPEPE
410 420 430 440 450
NNQMAISNNS QQSNEQTDPE PEENETKKES SVPMCQGELR HWKTGHYTLI
460 470 480 490 500
HDHSKAEFAL DLILYCGCEG WEPEYGGFTS YIAKGEDEEL LTVNPESNSL
510 520 530 540
ALVYRDRETL KFVKHINHRS LEQKKTFPNR TGFWDFSFIY YE
Length:542
Mass (Da):63,246
Last modified:October 1, 2002 - v1
Checksum:i8834BB200D5D8D85
GO
Isoform 2 (identifier: Q8N543-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-140: Missing.

Note: No experimental confirmation available.

Show »
Length:402
Mass (Da):46,861
Checksum:i8309970C9ED3AEB8
GO

Sequence cautioni

The sequence BAB13438.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB14880.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501S → G in BAB13967. (PubMed:14702039)Curated
Sequence conflicti112 – 1121I → V in BAB14226. (PubMed:14702039)Curated
Sequence conflicti343 – 3431E → G in BAB14880. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti173 – 1731P → S.1 Publication
Corresponds to variant rs34883368 [ dbSNP | Ensembl ].
VAR_032545

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 140140Missing in isoform 2. 1 PublicationVSP_025852Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046832 mRNA. Translation: BAB13438.1. Different initiation.
AK001688 mRNA. Translation: BAA91838.1.
AK022130 mRNA. Translation: BAB13967.1.
AK022752 mRNA. Translation: BAB14226.1.
AK024314 mRNA. Translation: BAB14880.1. Different initiation.
AC092140 Genomic DNA. No translation available.
BC032919 mRNA. Translation: AAH32919.1.
CCDSiCCDS10761.2. [Q8N543-1]
RefSeqiNP_060703.3. NM_018233.3.
UniGeneiHs.231883.

Genome annotation databases

EnsembliENST00000566157; ENSP00000457258; ENSG00000087263. [Q8N543-1]
GeneIDi55239.
KEGGihsa:55239.
UCSCiuc002ejb.3. human. [Q8N543-1]

Polymorphism databases

DMDMi74728942.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046832 mRNA. Translation: BAB13438.1 . Different initiation.
AK001688 mRNA. Translation: BAA91838.1 .
AK022130 mRNA. Translation: BAB13967.1 .
AK022752 mRNA. Translation: BAB14226.1 .
AK024314 mRNA. Translation: BAB14880.1 . Different initiation.
AC092140 Genomic DNA. No translation available.
BC032919 mRNA. Translation: AAH32919.1 .
CCDSi CCDS10761.2. [Q8N543-1 ]
RefSeqi NP_060703.3. NM_018233.3.
UniGenei Hs.231883.

3D structure databases

ProteinModelPortali Q8N543.
SMRi Q8N543. Positions 72-320.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120532. 34 interactions.
STRINGi 9606.ENSP00000337196.

Chemistry

DrugBanki DB00126. Vitamin C.

PTM databases

PhosphoSitei Q8N543.

Polymorphism databases

DMDMi 74728942.

Proteomic databases

MaxQBi Q8N543.
PaxDbi Q8N543.
PRIDEi Q8N543.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000566157 ; ENSP00000457258 ; ENSG00000087263 . [Q8N543-1 ]
GeneIDi 55239.
KEGGi hsa:55239.
UCSCi uc002ejb.3. human. [Q8N543-1 ]

Organism-specific databases

CTDi 55239.
GeneCardsi GC16P056488.
HGNCi HGNC:25585. OGFOD1.
HPAi HPA003215.
MIMi 615857. gene.
neXtProti NX_Q8N543.
PharmGKBi PA143485568.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG3751.
GeneTreei ENSGT00390000002349.
HOGENOMi HOG000007015.
HOVERGENi HBG056995.
InParanoidi Q8N543.
OMAi FSFVYYE.
OrthoDBi EOG7FBRHW.
PhylomeDBi Q8N543.
TreeFami TF105920.

Miscellaneous databases

ChiTaRSi OGFOD1. human.
GeneWikii OGFOD1.
GenomeRNAii 55239.
NextBioi 35527560.
PROi Q8N543.
SOURCEi Search...

Gene expression databases

Bgeei Q8N543.
CleanExi HS_OGFOD1.
ExpressionAtlasi Q8N543. baseline and differential.
Genevestigatori Q8N543.

Family and domain databases

InterProi IPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR019601. Oxoglutarate/Fe-dep_Oase_C.
IPR006620. Pro_4_hyd_alph.
[Graphical view ]
Pfami PF13640. 2OG-FeII_Oxy_3. 1 hit.
PF10637. Ofd1_CTDD. 1 hit.
[Graphical view ]
SMARTi SM00702. P4Hc. 1 hit.
[Graphical view ]
PROSITEi PS51471. FE2OG_OXY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O.
    DNA Res. 7:273-281(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT SER-173.
    Tissue: Embryo, Ovary and Teratocarcinoma.
  3. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. "OGFOD1, a member of the 2-oxoglutarate and iron dependent dioxygenase family, functions in ischemic signaling."
    Saito K., Adachi N., Koyama H., Matsushita M.
    FEBS Lett. 584:3340-3347(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF HIS-155.
  6. "OGFOD1, a novel modulator of eukaryotic translation initiation factor 2alpha phosphorylation and the cellular response to stress."
    Wehner K.A., Schutz S., Sarnow P.
    Mol. Cell. Biol. 30:2006-2016(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization."
    Ahmad Y., Boisvert F.M., Lundberg E., Uhlen M., Lamond A.I.
    Mol. Cell. Proteomics 11:M111.013680.01-M111.013680.15(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. Cited for: FUNCTION, CATALYTIC ACTIVITY.
  10. Cited for: FUNCTION, MUTAGENESIS OF ASP-157.

Entry informationi

Entry nameiOGFD1_HUMAN
AccessioniPrimary (citable) accession number: Q8N543
Secondary accession number(s): H3BUQ2
, Q9H7U5, Q9H9J9, Q9HA87, Q9HCG0, Q9NVB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2002
Last modified: November 26, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3