Q8N543 (OGFD1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1
EC=1.14.11.-

Alternative name(s):
Termination and polyadenylation 1 homolog

Gene names

Name:	OGFOD1
Synonyms:	KIAA1612, TPA1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	542 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Cofactor	Binds 1 Fe2+ ion per subunit By similarity. Ascorbate By similarity.
Subcellular location	Isoform 1: Cytoplasm Ref.5. Nucleus Ref.5. Isoform 2: Cytoplasm Ref.5.
Sequence similarities	Belongs to the TPA1 family. Contains 1 Fe2OG dioxygenase domain.
Sequence caution	The sequence BAB13438.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14880.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N543-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N543-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-140: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 542	542	2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1		PRO_0000288974
Regions
	Domain	134 – 239	106	Fe2OG dioxygenase
Sites
	Metal binding	155	1	Iron By similarity
	Metal binding	157	1	Iron By similarity
	Metal binding	218	1	Iron By similarity
	Binding site	228	1	2-oxoglutarate Potential
Natural variations
	Alternative sequence	1 – 140	140	Missing in isoform 2.		VSP_025852
	Natural variant	173	1	P → S. Ref.2 Corresponds to variant rs34883368 [ dbSNP | Ensembl ].		VAR_032545
Experimental info
	Sequence conflict	50	1	S → G in BAB13967. Ref.2
	Sequence conflict	112	1	I → V in BAB14226. Ref.2
	Sequence conflict	343	1	E → G in BAB14880. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 8834BB200D5D8D85	FASTA	542	63,246
	10 20 30 40 50 60 MNGKRPAEPG PARVGKKGKK EVMAEFSDAV TEETLKKQVA EAWSRRTPFS HEVIVMDMDP 70 80 90 100 110 120 FLHCVIPNFI QSQDFLEGLQ KELMNLDFHE KYNDLYKFQQ SDDLKKRREP HISTLRKILF 130 140 150 160 170 180 EDFRSWLSDI SKIDLESTID MSCAKYEFTD ALLCHDDELE GRRIAFILYL VPPWDRSMGG 190 200 210 220 230 240 TLDLYSIDEH FQPKQIVKSL IPSWNKLVFF EVSPVSFHQV SEVLSEEKSR LSISGWFHGP 250 260 270 280 290 300 SLTRPPNYFE PPIPRSPHIP QDHEILYDWI NPTYLDMDYQ VQIQEEFEES SEILLKEFLK 310 320 330 340 350 360 PEKFTKVCEA LEHGHVEWSS RGPPNKRFYE KAEESKLPEI LKECMKLFRS EALFLLLSNF 370 380 390 400 410 420 TGLKLHFLAP SEEDEMNDKK EAETTDITEE GTSHSPPEPE NNQMAISNNS QQSNEQTDPE 430 440 450 460 470 480 PEENETKKES SVPMCQGELR HWKTGHYTLI HDHSKAEFAL DLILYCGCEG WEPEYGGFTS 490 500 510 520 530 540 YIAKGEDEEL LTVNPESNSL ALVYRDRETL KFVKHINHRS LEQKKTFPNR TGFWDFSFIY YE
	Isoform 2 [UniParc]. Checksum: 8309970C9ED3AEB8	FASTA	402	46,861
	10 20 30 40 50 60 MSCAKYEFTD ALLCHDDELE GRRIAFILYL VPPWDRSMGG TLDLYSIDEH FQPKQIVKSL 70 80 90 100 110 120 IPSWNKLVFF EVSPVSFHQV SEVLSEEKSR LSISGWFHGP SLTRPPNYFE PPIPRSPHIP 130 140 150 160 170 180 QDHEILYDWI NPTYLDMDYQ VQIQEEFEES SEILLKEFLK PEKFTKVCEA LEHGHVEWSS 190 200 210 220 230 240 RGPPNKRFYE KAEESKLPEI LKECMKLFRS EALFLLLSNF TGLKLHFLAP SEEDEMNDKK 250 260 270 280 290 300 EAETTDITEE GTSHSPPEPE NNQMAISNNS QQSNEQTDPE PEENETKKES SVPMCQGELR 310 320 330 340 350 360 HWKTGHYTLI HDHSKAEFAL DLILYCGCEG WEPEYGGFTS YIAKGEDEEL LTVNPESNSL 370 380 390 400 ALVYRDRETL KFVKHINHRS LEQKKTFPNR TGFWDFSFIY YE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O. DNA Res. 7:273-281(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT SER-173. Tissue: Embryo, Ovary and Teratocarcinoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]	"Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization." Ahmad Y., Boisvert F.M., Lundberg E., Uhlen M., Lamond A.I. Mol. Cell. Proteomics 11:M111.013680.01-M111.013680.15(2012) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB046832 mRNA. Translation: BAB13438.1. Different initiation. AK001688 mRNA. Translation: BAA91838.1. AK022130 mRNA. Translation: BAB13967.1. AK022752 mRNA. Translation: BAB14226.1. AK024314 mRNA. Translation: BAB14880.1. Different initiation. BC032919 mRNA. Translation: AAH32919.1.
IPI	IPI00170429. IPI00402205.
RefSeq	NP_060703.3. NM_018233.3.
UniGene	Hs.231883.
3D structure databases
ProteinModelPortal	Q8N543.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000337196.
PTM databases
PhosphoSite	Q8N543.
Polymorphism databases
DMDM	74728942.
Proteomic databases
PaxDb	Q8N543.
PRIDE	Q8N543.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000566157; ENSP00000457258; ENSG00000087263.
GeneID	55239.
KEGG	hsa:55239.
UCSC	uc002ejb.3. human.
Organism-specific databases
CTD	55239.
GeneCards	GC16P056488.
HGNC	HGNC:25585. OGFOD1.
HPA	HPA003215.
neXtProt	NX_Q8N543.
PharmGKB	PA143485568.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG3751.
HOGENOM	HOG000007015.
HOVERGEN	HBG056995.
InParanoid	Q8N543.
OMA	ALLCHDD.
OrthoDB	EOG4B5P53.
Gene expression databases
ArrayExpress	Q8N543.
Bgee	Q8N543.
CleanEx	HS_OGFOD1.
Genevestigator	Q8N543.
Family and domain databases
InterPro	IPR005123. Oxoglu/Fe-dep_dioxygenase. IPR019601. Oxoglutarate/Fe-dep_Oase_C. IPR006620. Pro_4_hyd_alph. [Graphical view]
Pfam	PF13640. 2OG-FeII_Oxy_3. 1 hit. PF10637. Ofd1_CTDD. 1 hit. [Graphical view]
SMART	SM00702. P4Hc. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	OGFOD1. human.
DrugBank	DB00126. Vitamin C.
GenomeRNAi	55239.
NextBio	59266.

Entry information

Entry name

OGFD1_HUMAN

Accession

Primary (citable) accession number: Q8N543
Secondary accession number(s): Q9H7U5 , Q9H9J9, Q9HA87, Q9HCG0, Q9NVB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.