Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Actin filament-associated protein 1-like 2

Gene

AFAP1L2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in a signaling cascade by enhancing the kinase activity of SRC. Contributes to SRC-regulated transcription activation.1 Publication

GO - Molecular functioni

  • protein tyrosine kinase activator activity Source: HGNC
  • SH2 domain binding Source: HGNC
  • SH3 domain binding Source: HGNC
  • signaling adaptor activity Source: InterPro

GO - Biological processi

  • inflammatory response Source: HGNC
  • positive regulation of epidermal growth factor receptor signaling pathway Source: HGNC
  • positive regulation of interleukin-8 production Source: HGNC
  • positive regulation of transcription, DNA-templated Source: HGNC
  • regulation of interleukin-6 production Source: HGNC
  • regulation of mitotic cell cycle Source: HGNC
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Actin filament-associated protein 1-like 2
Short name:
AFAP1-like protein 2
Gene namesi
Name:AFAP1L2
Synonyms:KIAA1914, XB130
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:25901. AFAP1L2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HGNC
  • plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi4Y → F: Reduced interaction with SRC. 1 Publication1

Organism-specific databases

DisGeNETi84632.
OpenTargetsiENSG00000169129.
PharmGKBiPA162375773.

Polymorphism and mutation databases

BioMutaiAFAP1L2.
DMDMi68052360.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508051 – 818Actin filament-associated protein 1-like 2Add BLAST818

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei56PhosphotyrosineBy similarity1
Modified residuei408PhosphoserineBy similarity1
Modified residuei413PhosphotyrosineBy similarity1
Modified residuei484PhosphoserineBy similarity1

Post-translational modificationi

Tyrosine phosphorylated (by SRC).2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8N4X5.
PaxDbiQ8N4X5.
PeptideAtlasiQ8N4X5.
PRIDEiQ8N4X5.

PTM databases

iPTMnetiQ8N4X5.
PhosphoSitePlusiQ8N4X5.

Expressioni

Tissue specificityi

Detected in spleen and thyroid, and at lower levels in kidney, brain, lung and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000169129.
CleanExiHS_AFAP1L2.
ExpressionAtlasiQ8N4X5. baseline and differential.
GenevisibleiQ8N4X5. HS.

Organism-specific databases

HPAiHPA037843.

Interactioni

Subunit structurei

Interacts with SRC. Interacts with LCK when tyrosine phosphorylated.2 Publications

GO - Molecular functioni

  • SH2 domain binding Source: HGNC
  • SH3 domain binding Source: HGNC

Protein-protein interaction databases

BioGridi124161. 7 interactors.
IntActiQ8N4X5. 5 interactors.
MINTiMINT-2877327.
STRINGi9606.ENSP00000303042.

Structurei

Secondary structure

1818
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi359 – 364Combined sources6
Beta strandi367 – 375Combined sources9
Beta strandi380 – 383Combined sources4
Beta strandi390 – 396Combined sources7
Turni398 – 400Combined sources3
Beta strandi402 – 404Combined sources3
Beta strandi414 – 419Combined sources6
Beta strandi422 – 428Combined sources7
Helixi432 – 445Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2COFNMR-A354-447[»]
ProteinModelPortaliQ8N4X5.
SMRiQ8N4X5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8N4X5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini175 – 271PH 1PROSITE-ProRule annotationAdd BLAST97
Domaini353 – 447PH 2PROSITE-ProRule annotationAdd BLAST95

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili652 – 749Sequence analysisAdd BLAST98

Sequence similaritiesi

Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IEU6. Eukaryota.
ENOG4111CMN. LUCA.
GeneTreeiENSGT00390000003998.
HOVERGENiHBG106875.
InParanoidiQ8N4X5.
OMAiQWKSRWC.
OrthoDBiEOG091G03NB.
PhylomeDBiQ8N4X5.
TreeFamiTF332622.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR030113. AFAP.
IPR030115. AFAP1L2.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR14338. PTHR14338. 1 hit.
PTHR14338:SF4. PTHR14338:SF4. 1 hit.
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N4X5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERYKALEQL LTELDDFLKI LDQENLSSTA LVKKSCLAEL LRLYTKSSSS
60 70 80 90 100
DEEYIYMNKV TINKQQNAES QGKAPEEQGL LPNGEPSQHS SAPQKSLPDL
110 120 130 140 150
PPPKMIPERK QLAIPKTESP EGYYEEAEPY DTSLNEDGEA VSSSYESYDE
160 170 180 190 200
EDGSKGKSAP YQWPSPEAGI ELMRDARICA FLWRKKWLGQ WAKQLCVIKD
210 220 230 240 250
NRLLCYKSSK DHSPQLDVNL LGSSVIHKEK QVRKKEHKLK ITPMNADVIV
260 270 280 290 300
LGLQSKDQAE QWLRVIQEVS GLPSEGASEG NQYTPDAQRF NCQKPDIAEK
310 320 330 340 350
YLSASEYGSS VDGHPEVPET KDVKKKCSAG LKLSNLMNLG RKKSTSLEPV
360 370 380 390 400
ERSLETSSYL NVLVNSQWKS RWCSVRDNHL HFYQDRNRSK VAQQPLSLVG
410 420 430 440 450
CEVVPDPSPD HLYSFRILHK GEELAKLEAK SSEEMGHWLG LLLSESGSKT
460 470 480 490 500
DPEEFTYDYV DADRVSCIVS AAKNSLLLMQ RKFSEPNTYI DGLPSQDRQE
510 520 530 540 550
ELYDDVDLSE LTAAVEPTEE ATPVADDPNE RESDRVYLDL TPVKSFLHGP
560 570 580 590 600
SSAQAQASSP TLSCLDNATE ALPADSGPGP TPDEPCIKCP ENLGEQQLES
610 620 630 640 650
LEPEDPSLRI TTVKIQTEQQ RISFPPSCPD AVVATPPGAS PPVKDRLRVT
660 670 680 690 700
SAEIKLGKNR TEAEVKRYTE EKERLEKKKE EIRGHLAQLR KEKRELKETL
710 720 730 740 750
LKCTDKEVLA SLEQKLKEID EECRGEESRR VDLELSIMEV KDNLKKAEAG
760 770 780 790 800
PVTLGTTVDT THLENVSPRP KAVTPASAPD CTPVNSATTL KNRPLSVVVT
810
GKGTVLQKAK EWEKKGAS
Length:818
Mass (Da):91,300
Last modified:October 1, 2002 - v1
Checksum:i36040D6D09491DA8
GO
Isoform 2 (identifier: Q8N4X5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     766-769: Missing.

Show »
Length:814
Mass (Da):90,860
Checksum:i9C5F035A6B486D83
GO
Isoform 3 (identifier: Q8N4X5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-478: Missing.
     811-818: EWEKKGAS → VSSHSQPPLGPAEMSLR

Show »
Length:349
Mass (Da):38,300
Checksum:i8E2805F99A1A4DAF
GO
Isoform 4 (identifier: Q8N4X5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     135-135: N → NGHSGGFLPTGVPRWVQVPERVIYATITL
     766-769: Missing.

Show »
Length:842
Mass (Da):93,894
Checksum:i6E74C70CDB401E24
GO

Sequence cautioni

The sequence BAB67807 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13E → K in BAF84401 (PubMed:14702039).Curated1
Sequence conflicti224S → G in BAF84401 (PubMed:14702039).Curated1
Sequence conflicti567N → D in BAB55135 (PubMed:14702039).Curated1
Sequence conflicti596Missing in BAB55135 (PubMed:14702039).Curated1
Sequence conflicti605D → Y in BAB55135 (PubMed:14702039).Curated1
Sequence conflicti608L → Q in AAQ05765 (Ref. 1) Curated1
Sequence conflicti630D → G in BAF84401 (PubMed:14702039).Curated1
Sequence conflicti758V → L in AAQ05765 (Ref. 1) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050505138G → R.Corresponds to variant rs11196689dbSNPEnsembl.1
Natural variantiVAR_050506366S → R.Corresponds to variant rs7075067dbSNPEnsembl.1
Natural variantiVAR_050507522T → S.Corresponds to variant rs2781806dbSNPEnsembl.1
Natural variantiVAR_054214726E → K.Corresponds to variant rs11599051dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0142541 – 478Missing in isoform 3. 1 PublicationAdd BLAST478
Alternative sequenceiVSP_036211135N → NGHSGGFLPTGVPRWVQVPE RVIYATITL in isoform 4. 1 Publication1
Alternative sequenceiVSP_014255766 – 769Missing in isoform 2 and isoform 4. 2 Publications4
Alternative sequenceiVSP_014256811 – 818EWEKKGAS → VSSHSQPPLGPAEMSLR in isoform 3. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF474151 mRNA. Translation: AAQ05765.1.
AB067501 mRNA. Translation: BAB67807.1. Different initiation.
AL355302, AC005383, AL133384 Genomic DNA. Translation: CAI14967.1.
AL355302, AC005383, AL133384 Genomic DNA. Translation: CAI14968.1.
AL133384, AC005383, AL355302 Genomic DNA. Translation: CAI40128.1.
AL133384, AC005383, AL355302 Genomic DNA. Translation: CAI40129.1.
AK027470 mRNA. Translation: BAB55135.1.
AK291712 mRNA. Translation: BAF84401.1.
AK123108 mRNA. Translation: BAG53870.1.
BC024314 mRNA. Translation: AAH24314.1.
BC033212 mRNA. Translation: AAH33212.1.
CCDSiCCDS31286.1. [Q8N4X5-1]
CCDS31287.1. [Q8N4X5-2]
RefSeqiNP_001001936.1. NM_001001936.2. [Q8N4X5-1]
NP_001274753.1. NM_001287824.1.
NP_115939.1. NM_032550.3. [Q8N4X5-2]
UniGeneiHs.501106.

Genome annotation databases

EnsembliENST00000304129; ENSP00000303042; ENSG00000169129. [Q8N4X5-1]
ENST00000369271; ENSP00000358276; ENSG00000169129. [Q8N4X5-2]
GeneIDi84632.
KEGGihsa:84632.
UCSCiuc001lbn.5. human. [Q8N4X5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF474151 mRNA. Translation: AAQ05765.1.
AB067501 mRNA. Translation: BAB67807.1. Different initiation.
AL355302, AC005383, AL133384 Genomic DNA. Translation: CAI14967.1.
AL355302, AC005383, AL133384 Genomic DNA. Translation: CAI14968.1.
AL133384, AC005383, AL355302 Genomic DNA. Translation: CAI40128.1.
AL133384, AC005383, AL355302 Genomic DNA. Translation: CAI40129.1.
AK027470 mRNA. Translation: BAB55135.1.
AK291712 mRNA. Translation: BAF84401.1.
AK123108 mRNA. Translation: BAG53870.1.
BC024314 mRNA. Translation: AAH24314.1.
BC033212 mRNA. Translation: AAH33212.1.
CCDSiCCDS31286.1. [Q8N4X5-1]
CCDS31287.1. [Q8N4X5-2]
RefSeqiNP_001001936.1. NM_001001936.2. [Q8N4X5-1]
NP_001274753.1. NM_001287824.1.
NP_115939.1. NM_032550.3. [Q8N4X5-2]
UniGeneiHs.501106.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2COFNMR-A354-447[»]
ProteinModelPortaliQ8N4X5.
SMRiQ8N4X5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124161. 7 interactors.
IntActiQ8N4X5. 5 interactors.
MINTiMINT-2877327.
STRINGi9606.ENSP00000303042.

PTM databases

iPTMnetiQ8N4X5.
PhosphoSitePlusiQ8N4X5.

Polymorphism and mutation databases

BioMutaiAFAP1L2.
DMDMi68052360.

Proteomic databases

MaxQBiQ8N4X5.
PaxDbiQ8N4X5.
PeptideAtlasiQ8N4X5.
PRIDEiQ8N4X5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304129; ENSP00000303042; ENSG00000169129. [Q8N4X5-1]
ENST00000369271; ENSP00000358276; ENSG00000169129. [Q8N4X5-2]
GeneIDi84632.
KEGGihsa:84632.
UCSCiuc001lbn.5. human. [Q8N4X5-1]

Organism-specific databases

CTDi84632.
DisGeNETi84632.
GeneCardsiAFAP1L2.
HGNCiHGNC:25901. AFAP1L2.
HPAiHPA037843.
MIMi612420. gene.
neXtProtiNX_Q8N4X5.
OpenTargetsiENSG00000169129.
PharmGKBiPA162375773.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEU6. Eukaryota.
ENOG4111CMN. LUCA.
GeneTreeiENSGT00390000003998.
HOVERGENiHBG106875.
InParanoidiQ8N4X5.
OMAiQWKSRWC.
OrthoDBiEOG091G03NB.
PhylomeDBiQ8N4X5.
TreeFamiTF332622.

Miscellaneous databases

ChiTaRSiAFAP1L2. human.
EvolutionaryTraceiQ8N4X5.
GenomeRNAii84632.
PROiQ8N4X5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169129.
CleanExiHS_AFAP1L2.
ExpressionAtlasiQ8N4X5. baseline and differential.
GenevisibleiQ8N4X5. HS.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR030113. AFAP.
IPR030115. AFAP1L2.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR14338. PTHR14338. 1 hit.
PTHR14338:SF4. PTHR14338:SF4. 1 hit.
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAF1L2_HUMAN
AccessioniPrimary (citable) accession number: Q8N4X5
Secondary accession number(s): A8K6P7
, B3KVQ8, Q2UZW3, Q8TB54, Q96PX4, Q96SY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.